M****e
发帖数: 178 | 1 Hello,
I have a quick question about the terms used in the field of analytical
chemistry. Any input is really appreciated.
We are trying to determine whether a patient has taken a certain drug (
compliance) by examining the existence of drug metabolite in patient's
plasma via mass spectrometry. To evaluate how accurately this MS procedure
predict patient's compliance, we treat MS as an assay and patient's
compliance as gold standard to calculate sensitivity and specificity. But
reviewers said 'sensitivity' and 'specificity' are not proper terms used in
this field. I just wonder if there are some other quantity/term to measure
the procedure performance in this case.
Thanks! |
s**********8
发帖数: 25265 | 2 snsitivity and specificity more analytical chemistry term. what is the
question?
in
【在 M****e 的大作中提到】
: Hello,
: I have a quick question about the terms used in the field of analytical
: chemistry. Any input is really appreciated.
: We are trying to determine whether a patient has taken a certain drug (
: compliance) by examining the existence of drug metabolite in patient's
: plasma via mass spectrometry. To evaluate how accurately this MS procedure
: predict patient's compliance, we treat MS as an assay and patient's
: compliance as gold standard to calculate sensitivity and specificity. But
: reviewers said 'sensitivity' and 'specificity' are not proper terms used in
: this field. I just wonder if there are some other quantity/term to measure
|
M****e
发帖数: 178 | 3 Thanks!
It's a manuscript "An LC-MS assay for the semi-quantitative screening of
cardiovascular medications in human serum", submitted to Journal of
Chromatography A.
One of the reviewers gave the comments:
If the manuscript should be published in an analytical journal some
modifications have to be done. Sensitivity, specificity and accuracy are not
used as typical in analytical terms. Please rephrase.
I'm clueless about what the reviewer meant to say.
【在 s**********8 的大作中提到】
: snsitivity and specificity more analytical chemistry term. what is the
: question?
:
: in
|
s**********8
发帖数: 25265 | 4 tell him this is wrong. he seems not an expert in the area.
go get several just publish paper in that paper which use these terms.
go find FDA guidance for industry for bioanalytical method validation, use
it as reference
not
【在 M****e 的大作中提到】
: Thanks!
: It's a manuscript "An LC-MS assay for the semi-quantitative screening of
: cardiovascular medications in human serum", submitted to Journal of
: Chromatography A.
: One of the reviewers gave the comments:
: If the manuscript should be published in an analytical journal some
: modifications have to be done. Sensitivity, specificity and accuracy are not
: used as typical in analytical terms. Please rephrase.
: I'm clueless about what the reviewer meant to say.
|
s**********8
发帖数: 25265 | 5 i mean in that journal.
use
【在 s**********8 的大作中提到】
: tell him this is wrong. he seems not an expert in the area.
: go get several just publish paper in that paper which use these terms.
: go find FDA guidance for industry for bioanalytical method validation, use
: it as reference
:
: not
|
M****e
发帖数: 178 | 6 Sounds good. Many thanks!
use
【在 s**********8 的大作中提到】
: tell him this is wrong. he seems not an expert in the area.
: go get several just publish paper in that paper which use these terms.
: go find FDA guidance for industry for bioanalytical method validation, use
: it as reference
:
: not
|
s**********8
发帖数: 25265 | 7 also, complain to the responsive editor for pick the wrong reviewer.
if he/she denies, complain to chief editor.
【在 M****e 的大作中提到】
: Sounds good. Many thanks!
:
: use
|
S***x
发帖数: 2012 | 8 en, 2001 Bioanalytical Guidance
use
【在 s**********8 的大作中提到】
: tell him this is wrong. he seems not an expert in the area.
: go get several just publish paper in that paper which use these terms.
: go find FDA guidance for industry for bioanalytical method validation, use
: it as reference
:
: not
|
|
t******t
发帖数: 3045 | 9 In your case, sensitivity is the ability of your assay to tell "positive"
from "negative" and specificity is the confidence you have on your "positive
" to be a "true positive". Explain it to the reviewers if they do not
understand.
in
【在 M****e 的大作中提到】
: Hello,
: I have a quick question about the terms used in the field of analytical
: chemistry. Any input is really appreciated.
: We are trying to determine whether a patient has taken a certain drug (
: compliance) by examining the existence of drug metabolite in patient's
: plasma via mass spectrometry. To evaluate how accurately this MS procedure
: predict patient's compliance, we treat MS as an assay and patient's
: compliance as gold standard to calculate sensitivity and specificity. But
: reviewers said 'sensitivity' and 'specificity' are not proper terms used in
: this field. I just wonder if there are some other quantity/term to measure
|
M****e
发帖数: 178 | 10 Haha, will consider this resort...
【在 s**********8 的大作中提到】
: also, complain to the responsive editor for pick the wrong reviewer.
: if he/she denies, complain to chief editor.
|
|
|
M****e
发帖数: 178 | 11 Super! Thanks!
【在 S***x 的大作中提到】
: en, 2001 Bioanalytical Guidance
:
: use
|
M****e
发帖数: 178 | 12 Yeah, definitely need add more words to explain 'sensitivity' and '
specificity' themselves. Even the definitions from Wikipedia will do the
work. Thanks for the nice suggestion!
positive
【在 t******t 的大作中提到】
: In your case, sensitivity is the ability of your assay to tell "positive"
: from "negative" and specificity is the confidence you have on your "positive
: " to be a "true positive". Explain it to the reviewers if they do not
: understand.
:
: in
|
m**********2
发帖数: 207 | 13 Maybe this reviewer prefers to use LOL to depict sensitivity, selectivity
replace specificity,SD instead of accuracy?
not sure, but just a guess.
not
【在 M****e 的大作中提到】
: Thanks!
: It's a manuscript "An LC-MS assay for the semi-quantitative screening of
: cardiovascular medications in human serum", submitted to Journal of
: Chromatography A.
: One of the reviewers gave the comments:
: If the manuscript should be published in an analytical journal some
: modifications have to be done. Sensitivity, specificity and accuracy are not
: used as typical in analytical terms. Please rephrase.
: I'm clueless about what the reviewer meant to say.
|
M****e
发帖数: 178 | 14 Thanks. May I ask what LOL stands for?
【在 m**********2 的大作中提到】
: Maybe this reviewer prefers to use LOL to depict sensitivity, selectivity
: replace specificity,SD instead of accuracy?
: not sure, but just a guess.
:
: not
|
y*****s
发帖数: 1047 | 15 LOL=laugh out loud...
LOD=limit of detection |
t**n
发帖数: 4365 | 16 你是不是用了这几个词，但是用错了？
not
【在 M****e 的大作中提到】
: Thanks!
: It's a manuscript "An LC-MS assay for the semi-quantitative screening of
: cardiovascular medications in human serum", submitted to Journal of
: Chromatography A.
: One of the reviewers gave the comments:
: If the manuscript should be published in an analytical journal some
: modifications have to be done. Sensitivity, specificity and accuracy are not
: used as typical in analytical terms. Please rephrase.
: I'm clueless about what the reviewer meant to say.
|
t*******h
发帖数: 51 | 17 this sounds correct. BTW, not LOL, should be LOD, limit of detection.
Sensitivity and specificiy have very different defination in othet fields.
especially, you were talking about patients, should check out the exact
definitation in medical statistics. google ROC curve.
In the field of sensor, transducer and signal processing, sensitivity is
defined as the first derivative of the transfer function (output readout vs
input signal).
【在 m**********2 的大作中提到】
: Maybe this reviewer prefers to use LOL to depict sensitivity, selectivity
: replace specificity,SD instead of accuracy?
: not sure, but just a guess.
:
: not
|
M****e
发帖数: 178 | 18 Right. Just checked Bioanalytical Method Validation Guidance for Industry.
They use sensitivity/selectivity/accuracy to refer to different measurements
...
【在 t**n 的大作中提到】
: 你是不是用了这几个词，但是用错了？
:
: not
|
M****e
发帖数: 178 | 19 I think this is the point. We certainly use these terms as statistical
measures of performance, and we mentioned this in Statistical Analysis part.
I guess I need to somehow highlight the definitions... Thanks!
vs
【在 t*******h 的大作中提到】
: this sounds correct. BTW, not LOL, should be LOD, limit of detection.
: Sensitivity and specificiy have very different defination in othet fields.
: especially, you were talking about patients, should check out the exact
: definitation in medical statistics. google ROC curve.
: In the field of sensor, transducer and signal processing, sensitivity is
: defined as the first derivative of the transfer function (output readout vs
: input signal).
|
p**********g
发帖数: 378 | 20 这两个是method validation里的,你有做这个吗?
不然就LOD/LOQ?
in
【在 M****e 的大作中提到】
: Hello,
: I have a quick question about the terms used in the field of analytical
: chemistry. Any input is really appreciated.
: We are trying to determine whether a patient has taken a certain drug (
: compliance) by examining the existence of drug metabolite in patient's
: plasma via mass spectrometry. To evaluate how accurately this MS procedure
: predict patient's compliance, we treat MS as an assay and patient's
: compliance as gold standard to calculate sensitivity and specificity. But
: reviewers said 'sensitivity' and 'specificity' are not proper terms used in
: this field. I just wonder if there are some other quantity/term to measure
|
e****e
发帖数: 941 | 21 RE，楼主用的的和典型的分析术语有一点区别，偏向于流行病学和生物标志物领域，
reviewer可能是这个意思，觉得会让人误解
positive
【在 t******t 的大作中提到】
: In your case, sensitivity is the ability of your assay to tell "positive"
: from "negative" and specificity is the confidence you have on your "positive
: " to be a "true positive". Explain it to the reviewers if they do not
: understand.
:
: in
|
