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发帖数: 156 | 1 Postdoctoral positions available
A postdoctoral position is available in the Laboratory of Cellular and
Molecular Biology at the National Cancer Institute. A focus of current
studies is understanding the contribution of integrin adhesion complexes,
including focal adhesions and invadopodia, to the control of cancer cell
proliferation and survival. A class of proteins called the Arf GTPase-
activating proteins (Arf GAPs), which regulate the Ras-superfamily proteins
Arfs and integrin adhesions, have recently been found to control signaling
from the adhesions. The postdoctoral researcher will study the role of two
Arf GAPs with unique effects on signaling from integrin adhesion complexes
through the Ras pathways, including the PI-3-kinase and the MAPK pathways.
Some techniques that will be used include molecular biology, biochemistry
and microscope imaging such as TIRF, confocal and super resolution methods.
A postdoctoral position is available in the Laboratory of Cellular and
Molecular Biology at the National Cancer Institute to study the molecular
basis of the regulation of Arf GTPase-activating protein catalysis. Arf
GTPase-activating proteins, including ASAP1, catalyze the hydrolysis of GTP
bound to the Ras superfamily member Arf, converting Arf-GTP to Arf-GDP. The
Arf GAPs are efficient, with enzymatic power of greater than 108 M-1s-1 and
highly regulated, with 10,000 to 100,000-fold differences in the inactive
and active states. The expression of ASAP1 has also been correlated with
poor prognosis in cancers and invasive and metastatic behavior of cancer
cells. Thus, understanding the molecular basis of catalysis and regulation
of this class of enzymes is of interest to fundamental principles of control
of enzyme activity and to assessing the value of this class of proteins as
a therapeutic target. The postdoctoral researcher will study the structural
basis for catalysis by ASAP1 and its the regulation with the goal of
determining the mechanism at atomic resolution. The project is a
collaboration with crystallography, NMR and mass spectrometry labs. The
candidate will be responsible for kinetic analysis, mutational analysis and
spectroscopic experiments including fluorescence and circular dichroism and
will work closely with the collaborators.
A postdoctoral position is available in the Laboratory of Cellular and
Molecular Biology at the National Cancer Institute. The control of the
actin cytoskeleton and cell adhesions is critical for cell proliferation,
survival, proliferation and migration. Loss of appropriate regulation leads
to cancer and invasion and metastasis. The Arf GAPs are emerging as
critical of the actin cytoskeleton. Recently, Arf GAPs have been found to
directly bind to and regulate molecular motors, established regulators of
the cytoskeleton (Chen PW, Jian X, Heissler SM, Le K, Luo R, Jenkins LM,
Nagy A, Moss J, Sellers JR, Randazzo PA. J Biol Chem. 2016 Apr 1;291(14):
7517-26. doi: 10.1074/jbc.M115.701292. PMID: 26893376. Luo R, Chen PW,
Wagenbach M, Jian X, Jenkins L, Wordeman L, Randazzo PA. J Biol Chem. 2016
Aug 16. pii: jbc.M116.732479. PMID: 27531749). The postdoctoral researcher
will focus on the examination of ASAP family proteins’ interaction with
actin filaments and nonmuscle myosin2 and AGAP family proteins’ interaction
with kinesins. Using biochemical, structural and cell biological
approaches, the fellow will determine the basis for regulation of the
interaction, regulation of the motors and the consequences to cell behavior.
The three positions are located at NCI on the NIH Bethesda campus.
Information about fellowships at NCI can be found on the NCI website (
https://ccr.cancer.gov/training/postdoc).
To apply, send a letter of motivation and CV and three letters of
recommendation by email to:
Paul Randazzo, National Cancer Institute, Laboratory of Cellular and
Molecular Biology, Bldg 37 Room 2042, Bethesda, MD 20892: [email protected]/* */
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