h*******r
发帖数: 847 | 1 http://www.ncbi.nlm.nih.gov/m/pubmed/23284890/
CDA-2, a urinary preparation, inhibits lung cancer development through the
suppression of NF-kappaB activation in myeloid cell.
Wang X, et al. PLoS One. 2012.
Show full citation
Abstract
CDA-2 (cell differentiation agent 2), a urinary preparation, has potent anti
- proliferative and pro-apoptotic properties in cancer cells. However, the
mechanisms of tumor inhibitory action of CDA-2 are far from clear, and
especially there was no report on lung cancer. Here we demonstrate that CDA-
2 and its main component phenylacetylglutamine (PG) reduce the metastatic
lung tumor growth, and increases survival time after inoculation with Lewis
lung carcinoma (LLC) cells in a dose-dependent manner in C57BL6 mice.
Proliferative program analysis in cancer cells revealed a fundamental impact
of CDA-2 and PG on proliferation and apoptosis, including Bcl-2, Bcl-XL,
cIAP1, Survivin, PCNA, Ki-67 proteins and TUNEL assays. CDA-2 and PG
significantly reduced NF-κB DNA-binding activity in lung cancer cells and
in alveolar macrophages of tumor bearing mice and especially decreased the
release of inflammatory factors including TNFα, IL-6, and KC. Furthermore,
CDA-2 and PG decrease the expressions of TLR2, TLR6, and CD14, but not TLR1,
TLR3, TLR4, and TLR9 in bone-marrow-derived macrophages (BMDM) of mice
stimulated by LLC-conditioned medium (LLC-CM). Over-expressing TLR2 in BMDM
prevented CDA-2 and PG from inhibiting NF-κB activation, as well as
induction of TNFα and IL-6. TLR2:TLR6 complexes mediate the effect of NF-κ
B inactivation by CDA-2. In conclusion, CDA-2 potently inhibits lung tumor
development by reduction of the inflammation in lung through suppression of
NF-κB activation in myeloid cells, associating with modulation of TLR2
signaling. | c*********e
发帖数: 16335 | 2 kinky!
anti
CDA-
【在 h*******r 的大作中提到】
: http://www.ncbi.nlm.nih.gov/m/pubmed/23284890/
: CDA-2, a urinary preparation, inhibits lung cancer development through the
: suppression of NF-kappaB activation in myeloid cell.
: Wang X, et al. PLoS One. 2012.
: Show full citation
: Abstract
: CDA-2 (cell differentiation agent 2), a urinary preparation, has potent anti
: - proliferative and pro-apoptotic properties in cancer cells. However, the
: mechanisms of tumor inhibitory action of CDA-2 are far from clear, and
: especially there was no report on lung cancer. Here we demonstrate that CDA-
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