J**a
发帖数: 215 | 1 Movie:
http://www.nature.com/nmeth/video/moy2008/index.html
Review articles:
http://www.nature.com/nmeth/focus/moy2008/index.html
Nature Methods' Method of the Year 2008 goes to super-resolution
fluorescence microscopy. This series of articles—and the related movie—
showcase how these novel imaging methods came into their own in 2008 and the
incredible impact they promise to have in biological research. The Methods
to Watch feature provides a glimpse of future Methods of the Year and the
Reader' |
r******l
发帖数: 121 | 2 对第一个方法还是非常佩服的，毕竟从光学角度讲，暗斑的尺寸的确可以不受衍射极限
的限制，这一点和亮斑是很不同的。
对另一种基于统计的方法就不是很理解了，有哪位高人指点一下？ |
m****w
发帖数: 1304 | 3 你的这个说法是和巴比涅原理矛盾的。
【在 r******l 的大作中提到】
: 对第一个方法还是非常佩服的，毕竟从光学角度讲，暗斑的尺寸的确可以不受衍射极限
: 的限制，这一点和亮斑是很不同的。
: 对另一种基于统计的方法就不是很理解了，有哪位高人指点一下？
|
x*******n
发帖数: 185 | 4 the below is from wiki which explains how STED works
Stimulated Emission Depletion microscopy, or STED microscopy, is a technique
that uses the non-linear de-excitation of fluorescent dyes to overcome the
resolution limit imposed by diffraction with standard confocal laser
scanning microscopes and conventional far-field optical microscopes [1].
A confocal laser scanning microscope uses a focused laser beam to illuminate
a small part of the sample being observed. The laser is tuned to a
frequency
【在 r******l 的大作中提到】
: 对第一个方法还是非常佩服的，毕竟从光学角度讲，暗斑的尺寸的确可以不受衍射极限
: 的限制，这一点和亮斑是很不同的。
: 对另一种基于统计的方法就不是很理解了，有哪位高人指点一下？
|
x*******n
发帖数: 185 | 5 PALM, STORM
What fitting a PSF is to localization, photo-activated localization
microscopy (PALM) is to "resolution"—this term is here used loosely to mean
measuring the distance between objects, not true optical resolution. Eric
Betzig and colleagues developed PALM;[12] Xiaowei Zhuang at Harvard used a
similar techniques and calls it STORM: stochastic optical reconstruction
microscopy.[13] The basic premise of both techniques is to fill the imaging
area with many dark fluorophores that can be p |
