Postdoc版 - Postdoc positions at the Hormel Institute, University of Minnesota |
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l*******j
发帖数: 37 | 1 We are looking for multiple enthusiastic, highly self-motivated Ph.D.
candidates (recently graduated) with strong laboratory skills in molecular
biology, biochemistry, and protein expression/purification. Experience in
electron cryo-microscopy (grid preparation and data processing) or X-ray
crystallography (data processing, phase determination, model-building and
refinement) would be a plus, but not required.
My laboratory focuses on studying how transcription happens and how it is
regulated by various transcription factors. Transcription is the first and
central step of gene expression, in which the DNA template is copied into
RNA by the RNA polymerase (RNAP), and defects in transcription and its
regulation can lead to a variety of severe human diseases. The long-term
goal is to provide a structural basis for understanding the mechanisms of
transcription initiation, elongation and termination by the bacterial RNAP.
My research lab will study the transition of transcription from initiation
to termination phases, the regulation of different transcription stages by
various factors and the mechanisms by which RNAP translocates with
nucleotide addition. These objectives will be achieved by structural
determination of the RNAP in complex with functionally associated factors
and appropriate DNA or RNA substrates captured at various steps in the
processes, using cryo-electron microscopy (cryo-EM) and X-ray
crystallography, as well as by appropriate biochemical experiments.
Our earlier efforts have been published in the peer-reviewed journals such
as: Science 2017 (PMID: 29146813); Nucleic acids research 2017 (PMC5314768)
and 2021 (PMC8501970); PNAS 2015 (PMC4343176) and 2016 (PMC 4839411); EMBO J
2020 (PMC7360974); PLoS Biology 2020 (PMC7360974); Nature Communications
2021 (PMC8113463). In addition, we also have strong interests in determining
the structures of critical macromolecular complexes in SARS-CoV-2. The
relevant recent publications include Science 2021 (PMID: 34315827) and
Nature Communications 2022 (PMID: 35241675).
Interested applicants should send CV and contact information of three
references along with a research statement summarizing her/his research
interests and experience to Dr. Liu ([email protected]). Lab website: https:/
/www.hi.umn.edu/research/faculty/bin-liu-phd/ |
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