s*w
发帖数: 329 | 1 Oh. I think you can just ignore it and continue your experiment. Should be
OK. | p**t
发帖数: 36 | 2 Are those small colonies close to each other?
And the big ones are isolated?
Anyway it's not a big deal, as long as there's no contaminations. | t*****e
发帖数: 3 | 3
If amp resistant plant, the small one is the contaminant one without
the antibiotic selective plasmid.
You can see the small colonies grow up after more than 14-16 hours
incubation in the 37C.
such small colonies usually cicle the big colony.
If you see the cluster of small colonies and well dispersed big colonies,
It may be the result that you have plate out them equally. Insufficient
supplement, the clustered colonies cannot grow as well as they should be. |
|
