i just think about a reactino:
luciferase
luciferin + ATP -------------> &*(^*(%^*(& + florescence
so maybe u can add luciferin and luciferase into the PFK+ATP
and design a set of time point to measure the velocity of
the decrease of ATP.
m***e 发帖数: 482
2
I thought it may need to react to the product so that the detection can be
continuous...
Aldolase
fructose-1,6-bisphosphate------>glyceraldehyde-3-phosphate
+dihydroxyacetone phosphate
那些显色反应都忘光了。。。//cry
【在 a*****y 的大作中提到】 : i just think about a reactino: : luciferase : luciferin + ATP -------------> &*(^*(%^*(& + florescence : so maybe u can add luciferin and luciferase into the PFK+ATP : and design a set of time point to measure the velocity of : the decrease of ATP.
