r******i
发帖数: 1445 | 1 https://www.phe.gov/s3/dualuse/Documents/gof-qanda.pdf
US government official definition
“Gain of function” refers to any modification of a biological agent that
confers new or
enhanced activity. Typically, researchers mutate or alter genes and examine
the impact of
these modifications on a particular property or trait of the organism. For
example, some
investigators can modify influenza viruses in ways that enhance
pathogenicity and/or
transmissibility in order to better understand the origins and nature of
these traits at the
molecular level, as well as their pathogenesis in susceptible hosts. Since
influenza viruses
constantly evolve in nature, these gain-of-function studies may help predict
whether these
viruses could evolve naturally over time to acquire these new or enhanced
traits, and if so,
how the viruses might affect hosts and the kinds of medical countermeasures
that might be
most effective. Some gain-of-function studies may entail biosafety and
biosecurity risks that
require unique risk assessment and mitigation measures. | c*******e
发帖数: 5818 | 2 生物里一般来说，“gain-of-function”大多数是指 over-expression，就是替换
native promoter。
直接 modify gene sequence 过去比较少见，尤其针对 有virulence agent/pathogen
。， | r******i
发帖数: 1445 | 3 Your definition is very broad. In some sense, any deliberate modification of
DNA is "gain-of-function".
I think the ones of concern is "gain-of-function" on pathogens, that may
change the infectivity and may risk public health.
I believe Peter Daszak's research described here qualify "gain-of-function"
on pathogens.
https://youtu.be/IdYDL_RK--w?t=1782
pathogen
【在 c*******e 的大作中提到】
: 生物里一般来说，“gain-of-function”大多数是指 over-expression，就是替换
: native promoter。
: 直接 modify gene sequence 过去比较少见，尤其针对 有virulence agent/pathogen
: 。，
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