h*****G
发帖数: 113 | 1 大家好，小弟最近构件质粒，插入GFP到一个基因的intron，利用endogenous的
promoter来表达GFP。基本的思路是Slice acceptor sequence-2A peptide-GFP, 因为
intron之前的exon没有刚好in frame, 所以需要在SA和2A之间加入几个碱基。现在的问
题不知道具体的SA 序列，在Addgene上看了几个plasmid，都没有把具体的序列位置给
标出来，所以也搞不清楚。比如这段序列是addgene一个plasmid的，表明是SA－2A序列
，也知道［］中的是2A序列。但是前面的话具体那段是SA呢，如果我需要在SA和2A之间
插入连个碱基，应该在哪里插入呢？
gcaagcttctgacctcttctcttcctcccacagggcctcgagagatctggcagcg［
gagagggcagaggaagtcttctaac
atgcggtgacgtggaggagaatcccggccct ］
或者各位大侠有没有常用的SA序列呢？非常感谢帮助指导。 |
|
g********d
发帖数: 4174 | 2 Posted on Advocate.com March 15, 2011
Gay Parents More Accepted Than Single Moms
By Advocate.com Editors
A new survey shows that Americans tend to be more accepting of gay and
lesbian couples raising children than single parents.
One third of Americans say they are comfortable with a variety of family
situations, according to a study by the Pew Research Center. That group is
called "acceptors." Another third, called "skeptics" by researchers, have
mixed views on nontraditional family structures... 阅读全帖 |
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a***n
发帖数: 578 | 3 introduce Grubbs' current fancy chemistry:
polymer template.
basic idea is to have a substrate binding with certain sequence acceptor
sites. Those acceptor sites arrange in a pattern or a line. Then target
oligomers come in and sit on those acceptor sites. Add catalysts in, and
target polymer got sythensis.
hehe. pretty fancy. Interested guy search his papers. Don't know whether he
published this topic.
cyclic polymer.
He can make [ch2-ch2] as cyclic polymer. The reason to make this is because
i |
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M*******a
发帖数: 15 | 4 They are totally different stories.
Some dissimilatory metal-reducers can use As(V) as an electron acceptor and
reduce As(V) to As(III). Lots of papers are available. Some bacteria such as
Shewanella and Geobacter spp. can even reduce U(VI) to U(IV). These are
dissimilatory reduction.
The recent NASA discovery talks about incorporating an alternative element
of one of the so called "essential elements" for life in bacterial biomass.
In their experiments, oxygen was the electron acceptor. |
|
z********u
发帖数: 94 | 5 做的是bulk FRET，因为我想比较两种情况下的区别，然后两个情况的quenching稍有区
别但是efficiency差别很大，所以想能不能correct quenching然后得到更确切的结论
。这篇paper已经读过了，他区别了dynamic quenching和static quenching，但是没有
具体给dynamic quenching的校正。 ratio A就是 FRET acceptor/direct acceptor对
吧，这个要怎么校正呢？
多谢多谢啊。
single
2005 |
|
C*******e
发帖数: 4348 | 6 考虑一个大的蛋白复合物
比如是正方体，边长大于50埃
考虑是实心的
那么顶面的donor有可能传递能量到底面的acceptor么？
donor和acceptor标记都在表面，不在蛋白复合物内部
还有一般而言FRET可以检测到的距离是在哪个范围内？
如果说数据是10%-30%的话 |
|
l********e
发帖数: 415 | 7 google "gene trap"
I'm pretty sure that the insert contains a splicing acceptor site followed
by STOP codons. This would promote upstream exon to be spliced into the
insert, instead of the downstream exons. Thus, the mutant transcript would
contain multiple STOP codons that prevent translation of downstream mRNA
sequences.
Leakage happens when normal splicing occurs at low frequency, even in the
presence of the inserted splicing acceptor site.
Hope it helps. |
|
b**y
发帖数: 86 | 8 你说的没错，氢化一般都是在H2大大过量的情况下发生的。。。。如果是1：1就不知道
了；
不过你后面说的要是用 氢acceptor 代替氢气，反应就变了：不管氢acceptor是THF还
是O2，净反应就不同了。 |
|
a***n
发帖数: 578 | 9 don't understand why people buy these simple tricks. Gave me millions I will
set up far more fancy than this stupid trick.
The technique is too straightfoward.
as a guess:
X ----------------> O | acceptor
----------------> | O
reflective materials.
there are two lights: one is X ray, behind human body, there is an acceptor to
receive X ray images. Then that image was transfer to signal and project
toward that guy by some fluorescent light, |
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l****u
发帖数: 3130 | 10 姓 名： 袁 荃 Yuan _ Quan
出生年月：1982年2月
学历： 博士， 教授
专业方向：分析化学
电 话：
Email：y*********[email protected]
教育与研究经历
2000年9月– 2004年7月：武汉大学化学学士学位
2004年9月– 2009年7月：北京大学无机化学博士学位导师：严纯华院士
2009年9月-2011年12月：佛罗里达大学化学系博士后谭蔚泓教授课题组
2011年12月-现在：武汉大学化学学院教授
代表性成果
1.Quan Yuan, Yun-Fei Zhang, Ruo-Wen Wang, Chao-Lin Du, Emir Yasun, Yan Chen,
and Wei-Hong Tan* “Using Silver Nanowire Antennas to Enhance the
Conversion Efficiency of Photoresponsive DNA Nanomotors” Proc. Natl. Acad.
Sci. U. S. A. 2011, 108, 9331.
2.Quan Yuan, Hao-Hong ... 阅读全帖 |
|
R****a
发帖数: 6858 | 11 人口過剩威脅遠超核武器；人口生滅的因與果；解決垃圾人口成當務之急 PART - 3
分類：Toxic Food 研究所
2012/05/22 23:02
分享 FacebookPlurkYAHOO!
http://tw.myblog.yahoo.com/transfinite-universe/article?mid=736
【POLICY FOLLOW-UP AND COORDINATION】
39. This world wide population strategy involves very complex and
difficult questions. Its implementation will require very careful
coordination and specific application in individual circumstances. Further
work is greatly needed in examining the mix of our assistance strategy and
its most effic... 阅读全帖 |
|
G***G
发帖数: 16778 | 12 why not directly look for sperm acceptor (surrogate mother) instead of
cell donator?
50
的这个年龄段一般都还没有生过小孩吧, 更年期到也就是说绝经了, 也就是说不能生育
了, 那如果这样等于别人为了你生小孩, 自己却以后生不了了, 因为现在大家都是晚结
婚晚生的啊. 那真是造劣了 |
|
m******n
发帖数: 898 | 13 ：）幼吾幼以及人之幼
Re这个站内捐奶的模式。我还颇花了一些精力研究处理多余奶的方法。目前社会上主要
有三种方式：
1. Milk bank donation. 这个我也在另一个回帖中提到了，national milk bank服务
最好，但有伪non-profit之嫌。north America Breast milk association是non
profit, 但需要自己去验血，自己去买干冰寄奶等等各种麻烦。与此类似的local
hospital donation也是如此
2. Online private sharing. 像Eats on feets，人们在网上贴供需信息。但是陌生人
之间share body fluid, 真的很risky. 那个网站建议接受捐助方出资要求捐助方去验
血。
3. Milk sale. 这个最奇怪啦，而且还有很多变态的男人买，不说也罢
对于日产50+的奶牛们来说，多余的奶是负担，孩子总有新鲜的奶喝，冻奶就显得很鸡
肋。除去应付特殊情况的存货以外，剩下的奶扔了可惜留着占地。站内共享的好处在于
donor和acceptor的人品和家庭情况基本可以... 阅读全帖 |
|
h*******u
发帖数: 160 | 14 Title: Control of electron transfer kinetics: Models for medium
reorganization and donor-acceptor coupling
Author(s): Newton, MD
Source: ELECTRON TRANSFER-FROM ISOLATED MOLECULES TO BIOMOLECULES, PT 1
Volume: 106 Pages: 303-375 Published: 1999
Times Cited: 93
Thanks a lot
w********[email protected] |
|
g********e
发帖数: 1638 | 15 208 邬俊波
(MSE) 男 1977/08/20 中国华能集团公司 工程与材料科学 2010年
06月毕业于[美国]斯坦福大学 [美国]SunPower公司 研发主管工程师/R&D Staff
Engineer
名校的威力太大了，这位千青有自己的主页，文章少的很。
http://peumansgroup.stanford.edu/Junbo_Wu
Junbo Wu
Contact Information
Email: wujunbogmailcom
Mobile: (650) 704-4362
Office: CISX 218X
Contact Address: CISX (Paul J. Allen Building), Stanford University, 420 Via
Palou Mall, Stanford, CA 94305
Research Project
Organic Solar Cells. Semiconductor device physics, Material proce... 阅读全帖 |
|
l****u
发帖数: 3130 | 16 【 以下文字转载自 Military 讨论区 】
发信人: laodou (别拿豆包不当干粮), 信区: Military
标 题: 武汉大学80后美女袁荃教授走红
发信站: BBS 未名空间站 (Wed Feb 29 14:21:37 2012, 美东)
姓 名： 袁 荃 Yuan _ Quan
出生年月：1982年2月
学历： 博士， 教授
专业方向：分析化学
电 话：
Email：y*********[email protected]
教育与研究经历
2000年9月– 2004年7月：武汉大学化学学士学位
2004年9月– 2009年7月：北京大学无机化学博士学位导师：严纯华院士
2009年9月-2011年12月：佛罗里达大学化学系博士后谭蔚泓教授课题组
2011年12月-现在：武汉大学化学学院教授
代表性成果
1.Quan Yuan, Yun-Fei Zhang, Ruo-Wen Wang, Chao-Lin Du, Emir Yasun, Yan Chen,
and Wei-Hong Tan* “Using Silver Nanowire Antennas to Enhance th... 阅读全帖 |
|
N******e
发帖数: 102 | 17 小额刷卡这一点我原来没有想到，很有道理。那我就只考虑收现金的了。现在有bill
acceptor 可以买到，就是不知道怎么把它们联网，远程查询收了多少钱。 |
|
p**********u
发帖数: 15479 | 18 cash wise: the total IPO is about 300 million share. Each share is at $35.
That is $10Billion in cash. But, they might have 90% of stocks on hand.
Many mutual funds are located at west coast. silicon guy is the ultimate
plate acceptor, stock or women. |
|
m********h
发帖数: 405 | 19 下次回答老板 minnow is donor, bass is acceptor ! |
|
d*****0
发帖数: 68029 | 20 【 以下文字转载自 Military 讨论区 】
发信人: laodou (别拿豆包不当干粮), 信区: Military
标 题: 武汉大学80后美女袁荃教授走红
发信站: BBS 未名空间站 (Wed Feb 29 14:21:37 2012, 美东)
姓 名： 袁 荃 Yuan _ Quan
出生年月：1982年2月
学历： 博士， 教授
专业方向：分析化学
电 话：
Email：y*********[email protected]
教育与研究经历
2000年9月– 2004年7月：武汉大学化学学士学位
2004年9月– 2009年7月：北京大学无机化学博士学位导师：严纯华院士
2009年9月-2011年12月：佛罗里达大学化学系博士后谭蔚泓教授课题组
2011年12月-现在：武汉大学化学学院教授
代表性成果
1.Quan Yuan, Yun-Fei Zhang, Ruo-Wen Wang, Chao-Lin Du, Emir Yasun, Yan Chen,
and Wei-Hong Tan* “Using Silver Nanowire Antennas to Enhance th... 阅读全帖 |
|
f*****n
发帖数: 970 | 21 though he has a gf, he doesn't have experience. since he is a passive
acceptor in his romance period. |
|
r*****y
发帖数: 507 | 22 也许那是因为SCI会议的组织者也编了个SCI CS paper Acceptor的程序。
输入：pdf (or ps, or word file), 输出： rejected or accepted.
呵呵。 |
|
b***i
发帖数: 3043 | 23 有一个类，TCPServer{
public: TCPServer(asio::io_service& io_service, const tcp::endpoint& listen_
endpoint){...}
private:
asio::io_service& io_service_;
asio::ip::tcp::acceptor acceptor_;
TCPListener listener_;
};
这个类是boost的一个例子。我的代码需要三个变量，放在TCPServerManager里面，为
简单起见，我定义三个static变量。
TCPServerManager{
static TCPServer eth0, eth1, wifi;
static void start(){...}
};
那么，在start里面，我根据boost的例子，用TCPServer s(io_service, listen_
endpoint);定义了一个变量。怎么把这个变量变成我的eth0,eth1, wifi呢？
在定义eth0的那个地方，需要的listen_endpoint还没有好，就... 阅读全帖 |
|
x*****o
发帖数: 441 | 24 你这个实验具体是想得出什么结论呢? 如果要求不高我们一般都不校正. 你是做single
molecule FRET 吗? 做这个的人从来不校正的说.
以前实验室做得bulk FRET的实验,就是简单的测Ratio A来校正,操作很简单.
Z.K. Majumdar, R.P. Hickerson, H.F. Noller and R.M. Clegg, Measurements of
Internal Distance Changes of the 30 S Ribosome Using FRET with Multiple
Donor–Acceptor Pairs: Quantitative Spetroscopic Methods, J. Mol. Biol (2005
) 351, 1123–1145. |
|
l**********1
发帖数: 5204 | 25 你老板没让你事先设计 reporter+ cassette modified vector 就做plasmid then
BAC transgene to Mouse 啦?
then 要证明Your transgene（insert）整合到特定locus
if yes plus next Long-range PCR does not work well.
return to start point then do below steps or similar steps:
Generation of knockin mice
Bacterial artificial chromosome (BAC) clone RP23-135A18
containing mouse genomic DNA encoding exon 1 of Zeb1 was
digested with PacI/SphI and SphI to yield 7.6-kb and 2.6-kb
homology arms, respectively, for targeting constructs (Figur... 阅读全帖 |
|
|
s********9
发帖数: 132 | 27 而且VMD是免费的.pymol现在收费了.
再问下:
1:homology model用哪个好? SPDV可以,不知道哪个好些? 文献上面说的homology的定量(比
如40% 60%是怎么来的? 是根据structural seq alignment 数完全相同的氨基酸数量除以总
数?还是别的?clustalw2是不给量化结果的.)
2:1)关于氢键,做结构的人文章里面到底donor atom 和acceptor atom离多远才算是可信? 我
前面用spdv似乎是定在3.5A.可是看一个文章里面描述的一个结构里面所谓重要的氢键已经4A以上
了.
2)一般说是N(O)-H...N(O),可是又看到有的文章里面说说C-H...N(o),这个氢键难道就是个
任人打扮的小姑娘?
3)如果在结构里面看到某个氨基酸的side chain和别的氨基酸有H bond,而我打算mutate这个
amino acids,一个结构生物学的人会怎么去推测这个mutation whether or to what
extent will affect the overall structure o... 阅读全帖 |
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C******2
发帖数: 97 | 28
vectors
The construction is modified and derived from pRetrosuper which is used for
random mutation in host genome based on gene-trap. Truncated 3’LTR
fragment is promoter activaty lost. The idea is host genome will be mutated
by insertion because the existence of splicing acceptor(SA) fragment thus
generate a fusion RNA containing cherry CDS. Fusion florescence protein can
be detected in some case when insertion occurs as an in frame manner. The
insertion will also make the infected cells be... 阅读全帖 |
|
s******9
发帖数: 283 | 29 NCBI protein database一般都有标注吧。
FEATURES Location/Qualifiers
source 1..503
/organism="Homo sapiens"
/db_xref="taxon:9606"
gene 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
Protein 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
... 阅读全帖 |
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l********e
发帖数: 415 | 30 如果你放splicing acceptor site的话，的确会 ... |
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A******y
发帖数: 2041 | 31 Most people look at GSH depletion because the cells already have a lot of it
. Some cells if you treated them with high glutamate, it will block
cysteine uptakes and deplete its GSH level. Your probe has a Micheal's
acceptor, so I you will have toxicity around 10s microM, but it is probably
okay to do short term live cell imagining.
I wounder if you treated with certain compounds, would you be able to
determine specific GSH pool depletion because there is a cyto- and a mito-
pool of GSH.
Tsie... 阅读全帖 |
|
p******n
发帖数: 55 | 32 http://www.grc.org/amc.aspx
熟悉的名字不少啊
2016 Lecturers
◾Nieng Yan, Membrane Transport Proteins
◾Xiaowei Zhuang, Synaptic Transmission
◾Christopher J. Chang, Metals in Medicine
◾Mikhail Lukin, Quantum Science
2015 Lecturers
◾Karl Deisseroth, Amygdala in Health & Disease
◾David M. Sabatini, Lysosomal Diseases
◾Laura Kiessling, Carbohydrates
◾Gerbrand Ceder, Physical Metallurgy
◾Liang Fu, Superconductivity
2014 Lecturers
◾Kristi S. Anseth, Si... 阅读全帖 |
|
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n********1
发帖数: 165 | 34 卖pp的阉狗真会意淫...You can only function as a dick acceptor. 你最近是不是
舔你老板的合作者舔习惯了，以至于看到之前你不屑的曹都要扑上来跪舔下 lol
disguting
shrink |
|
d********m
发帖数: 3662 | 35 lol
dick acceptor? you just watched your mother accepting too many dicks. poor
little kid. what a miserable life you were living.
by the way, your mother asked me to tell you shut the fuck up because I was
fucking the brain out of her. I must say, she is a very professional whore,
much worthier than little maggot like you. |
|
a******w
发帖数: 898 | 36 热力学不懂，不过你说的那个酮用Wilkinson氢化是可逆的反应。氢气压力大的时候酮
转化成醇，如果用 氢acceptor 代替氢气就可以把醇氧化成酮或醛,也叫做氢转移反应
are |
|
a******w
发帖数: 898 | 37 哦，我这里没说清楚，氢acceptor 是指缺电子双键，或者羰基，这类反应叫做氢转移
反应，用Rh 或者 Ru催化，净反应就是（举例来说）用异丙醇做氢源，最后把异丙醇氧
化成丙酮。这类反应的应用和变化非常广泛，也是非常hot topic。
我仅是指，Rh, Ru, (包括威尔森金催化剂)既可以氢化酮成醇，也可以氧化醇成酮，这是一个可逆反应。 |
|
n*****t
发帖数: 32 | 38 什么是hydride acceptor? Na+?
are |
|
s********n
发帖数: 235 | 39 Title: PROTONIC CONDUCTION IN ACCEPTOR-DOPED KTAO3 CRYSTALS
Author(s): LEE, WK; NOWICK, AS
Source: SOLID STATE IONICS Volume: 18-9 Pages: 989-993 Part: 2
Published: JAN 1986
x********[email protected]
many thanks! |
|
s***m
发帖数: 6197 | 40 只要输入一个compound的chemical structure
就能自动识别那些atoms是hydrogen bond donor或者acceptor
有这样的软件吗？
谢谢！ |
|
|
f******n
发帖数: 137 | 42 “Synthesis of Polymethacrylate Derivatives Containing Conducting
Diphenyldithiophene as Pendant Side Chain”Reichmanis, Elsa; Polymer
Preprints, ACS Fall Conference, 2011
Alternating and Diblock Donor–Acceptor Conjugated Polymers Based on
Diindeno[1,2-b:2′,1′-d]thiophene Structure: Synthesis, Characterization,
and Photovoltaic Applications, Volume 5, Issue 12, pages 2483–2492,
December 3, 2010
http://onlinelibrary.wiley.com/doi/10.1002/asia.201000506/abstr |
|
a*********r
发帖数: 14 | 43 对不起，我还真是给武大化学系留了面子。
莫非你就是其中一件丑事的主角，干嘛一触即发，破口大骂呢？
袁荃做的是还不错，但不意味武大化学系就有多好了。
举例来说，即使袁荃和另外一位新招的楚天特聘教授傅磊相比，也能看出武大化学系在其中有没有猫腻了。
首先补充一点：袁荃的老公叫廖蕾，也是武大物理系新招的楚天特聘教授，可以作为很好的对照体系。
比比三人的publication和经历，就能明显看出水平之比是:
廖蕾>袁荃>傅磊
但从袁荃却没有评上楚天特聘。
廖蕾
http://physics.whu.edu.cn/node/557
1. Y. Z. Long, L. Liao*, X. F. Duan, Z. Y. Fan*, Large-scale Integration of Semiconductor Nanowires for High Performance Flexible Electronics ACS Nano (In press)
2. L. Liao, J. W. Bai, R. Chen, H. L. Zh... 阅读全帖 |
|
a*********r
发帖数: 14 | 44 对不起，我还真是给武大化学系留了面子。
莫非你就是其中一件丑事的主角，干嘛一触即发，破口大骂呢？
袁荃做的是还不错，但不意味武大化学系就有多好了。
举例来说，即使袁荃和另外一位新招的楚天特聘教授傅磊相比，也能看出武大化学系在其中有没有猫腻了。
首先补充一点：袁荃的老公叫廖蕾，也是武大物理系新招的楚天特聘教授，可以作为很好的对照体系。
比比三人的publication和经历，就能明显看出水平之比是:
廖蕾>袁荃>傅磊
但从袁荃却没有评上楚天特聘。
廖蕾
http://physics.whu.edu.cn/node/557
1. Y. Z. Long, L. Liao*, X. F. Duan, Z. Y. Fan*, Large-scale Integration of Semiconductor Nanowires for High Performance Flexible Electronics ACS Nano (In press)
2. L. Liao, J. W. Bai, R. Chen, H. L. Zh... 阅读全帖 |
|
d********y
发帖数: 176 | 45 可能啊，有donor,有acceptor，为什么不可能？
要用红外来证明是不是有氢键就比较hand-waving，估计不能下特别强的结论，因为只
要在水里，就会和水形成氢键，谁知道这个氢键是和水形成的还是和这些N-OH 或者 P-
OH形成的。
图1 在哪里？ |
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b********d
发帖数: 720 | 46 对于hydrogen bond acceptor来说，不是应该孤对电子越多，电负性越大的越好吗？ |
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C*****G
发帖数: 81 | 47 1. title :Optical properties of Si-, Ge- and Sn-doped GaN
By: Shikanai, A; Fukahori, H; Kawakami, Y; et al.
Conference: International Conference on Shallow-Level Centers in
Semiconductors Location: WARSAW, POLAND Date: 2002
PHYSICA STATUS SOLIDI B-BASIC RESEARCH Volume: 235 Issue: 1 Pages: 26-
30 Published: JAN 2003
link:
http://onlinelibrary.wiley.com/doi/10.1002/pssb.200301525/abstr
2.Calculations of acceptor ionization energies in GaN
By: Wang, F; Chen, AB
PHYSICAL REVIEW B Volu... 阅读全帖 |
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f*******o
发帖数: 43 | 48 不客气
1） COD的概念是根据electron transfered --折算成氧气的量
2）在转化成COD单位的时候，
所有的chemical凡是消耗氧气的，都是正的（electron donor)
比如污水的COD的值mg/L，就是耗了多少氧气mg/L.
otherwise, electron acceptor, 都是负的;
所以对于氧气，就是-1
反硝化反应，同理，nitrate-->nitrogen,系数也应该是负的
你可以看一下这本书的p62-63
这是google book 的链接
http://books.google.com/books?id=qsIB-
eOBZ6wC&printsec=frontcover&dq=biological+wastewater+treatment&hl=en&ei=
bjyaTKiFGdP_nQfFyK3aDw&sa=X&oi=book_result&ct=book-
thumbnail&resnum=1&ved=0CDwQ6wEwAA#v=onepage&q&f=false |
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f*******o
发帖数: 43 | 49 差不多就是这个意思了
when the equation was in COD unit,
it's balanced in electron and COD mass
per unit of substrate was consumed, then Y unit of biomass was
synthesized, at the same time (1-Y) O2 was needed as electron acceptor. |
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a***n
发帖数: 578 | 50 get well defined structure. You can choose exact sequence by using different
acceptor sites.
Ideally, it can make man-made DNA or very complex bio-molecule or whatever.
this is big bucks funded. the name is recognization process? don't know.
cooperate with Prof. Stoddard at UCLA.
famous.
he
because
polymer. |
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