g*********3
发帖数: 177 | 1 1. Do we know any other signal pathways mediating amino acid response?
发现大部分关于amino acid的研究是关于mTOR的和mTOR相关的。
2. 上次版上有人提到了amino acid receptor是个悬而未决的问题，不知道是哪位牛提
到的。能展开说说吗？自己查了一下，确实研究的不是很多。 |
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C**********s
发帖数: 1282 | 3 haha......cracks me up :)
发信人: becomingjane (单腿着地的梦想家), 信区: LosAngeles
标 题: Re: aminos are terrible
发信站: BBS 未名空间站 (Sun Jun 27 15:58:43 2010, 美东)
你应该对他说你的partner不喜欢玫瑰 |
|
r**a
发帖数: 121 | 4 GCN2 patheay also mediates amino acid response...free tRNA responsing signal |
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t*****m
发帖数: 73 | 5 需要有确切amino acid sequence的Monoclonal antibody，作为mass spec的标准物质
。无所谓biological specificity。
哪里能买到？ |
|
c*****t
发帖数: 198 | 6 【 以下文字转载自 Biology 讨论区 】
发信人: compact (support), 信区: Biology
标 题: how to measure the spatial distribution of amino acids, like lysine
发信站: BBS 未名空间站 (Tue Jul 1 13:42:59 2008)
other than C14 isotope measurement. Thank you. |
|
发帖数: 1 | 7 求电子版书Salts of Amino Acids: Crystallization, Structure and Properties |
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c*****t
发帖数: 198 | 8 【 以下文字转载自 Biology 讨论区 】
发信人: compact (support), 信区: Biology
标 题: how to measure the spatial distribution of amino acids, like lysine
发信站: BBS 未名空间站 (Tue Jul 1 13:42:59 2008)
other than C14 isotope measurement. Thank you. |
|
c*****t
发帖数: 198 | 9 【 以下文字转载自 Biology 讨论区 】
发信人: compact (support), 信区: Biology
标 题: how to measure the spatial distribution of amino acids, like lysine
发信站: BBS 未名空间站 (Tue Jul 1 13:42:59 2008)
other than C14 isotope measurement. Thank you. |
|
|
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w*******y
发帖数: 60932 | 12 Optimum Nutrition Essential Amino Energy for sale on Amazon for $13.49.
Free 2-day Shipping w/ Amazon Prime.
The best deal I have gotten previously is $12.69 from Amazon w/ S&S.
Unfortunately, S&S no longer available for this item. For $0.80 more, this
is still a good deal!
Link:
http://www.amazon.com/gp/product/B003X5LM1U/ref=ox_sc_act_title
For people who have never used this before:
The directions say anywhere from 2-6 scoops, however, I use barely 1 scoop
and it does the job for me. Don'... 阅读全帖 |
|
w*******y
发帖数: 60932 | 13 I know that this isn't the lowest price in recent history. This was $13 and
change through Subscribe and Save a month or so ago. However, with a $3 off
coupon that just popped up today, $16.75 shipped (if you have Prime) is a
pretty good price for this stuff if you need it now (like I do - suddenly my
wife doesn't want to work out without the stuff as she LOVES working out
with it). It's $35 at her gym and it seems to be around $20 shipped - at
best - everywhere else (the one exception I've foun... 阅读全帖 |
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f**d
发帖数: 768 | 14 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
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c****l
发帖数: 1086 | 15 Lots of membrane amino acid transporters has been identified!! You can
easily find a review paper on this like the following one.
Amino Acid Transport Across Mammalian Intestinal and Renal Epithelia
http://physrev.physiology.org/content/88/1/249.short
The transport of amino acids in kidney and intestine is critical for the
supply of amino acids to all tissues and the homeostasis of plasma amino
acid levels. This is illustrated by a number of inherited disorders
affecting amino acid transport in ... 阅读全帖 |
|
c****l
发帖数: 1086 | 16 Lots of membrane amino acid transporters has been identified!! You can
easily find a review paper on this like the following one.
Amino Acid Transport Across Mammalian Intestinal and Renal Epithelia
http://physrev.physiology.org/content/88/1/249.short
The transport of amino acids in kidney and intestine is critical for the
supply of amino acids to all tissues and the homeostasis of plasma amino
acid levels. This is illustrated by a number of inherited disorders
affecting amino acid transport in ... 阅读全帖 |
|
C*I
发帖数: 4736 | 17 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 18 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 19 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
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G****e
发帖数: 1912 | 20 https://www.theepochtimes.com/scientific-puzzles-surrounding-the-wuhan-novel
-coronavirus_3225405.html
Scientific Puzzles Surrounding the Wuhan Novel Coronavirus
BY YUHONG DONG February 3, 2020 Updated: February 6, 2020FONT BFONT SText
size Print
Analysis
The sudden outbreak of the Wuhan Novel Coronavirus (2019-nCoV) has resulted
in all of China’s Hubei Province and three major cities in Zhejiang
Province being subjected to quarantine. Other nations are anxiously trying
to get their people out o... 阅读全帖 |
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c**i
发帖数: 6973 | 21 About--and continuing from--the previous posting attached below).
Correction:
(a) "(2) Then there are essential amino acids." The "amino" should be "fatty
."
(b) Bacteria do not make an essential fatty acid. Fungus does.
Note:
(a) The carbons in the chain (as opposed to occasional branches) of fatty
acids are counted in the same manner as those of amino acids: alpha, beta,
gamma and so on. But fatty acids can be long-chain, more than 20 carbons. To
refer to the other end (opposite from the alpha... 阅读全帖 |
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b*******t
发帖数: 33714 | 22 ppt直接导出来的，格式有点乱，不过有耐心的话看看还挺有意思的。
==================================
Part 3: Arts and Methods of Kitchen Arts
TOPIC: MEAT
Cooking meat
Cooking meat is all about producing the right texture and flavours.
Texture concerns the structure of meat.
Flavours are all about chemistry –the Maillard reaction.
Components of muscle
Water: 75%
Protein: 18%
Fat: ~4-10%, varies among different types of meat
Carbohydrate: just over 1%
Vitamins, minerals, various organic compounds…
Sliding-filament model of muscle... 阅读全帖 |
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c**i
发帖数: 6973 | 23 You wrote, "Soy is the only plant that offer complet protein."
It is incorrect.
(1) First and foremost, introduction to amino acids.
There are twenty amino acids that are encoded by genetic codes. There are
additional, minor ones that result from biochemical modification AFTER
assembly of amino acids.
http://en.wikipedia.org/wiki/Genetic_code
(codon)
(a) "In an alpha amino acid, the amino group is attached to the carbon atom
immediately adjacent to the carboxylate group (the α–carbon)."
http://e... 阅读全帖 |
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R******d
发帖数: 5739 | 24 Let me rephrase my words.
All the biological process have to abide the law of chemistry.
It does not matter what the origin of a biological process or how it began,
you can say RNA, protein, sugar, or whatever. You get to explain how these
molecules formed in nature in the first place.
Please do not just Wiki some topic, and then post it here because a lot of
them have erroneous messages. The belowing so call "well-known" Miller
experiment did not reproduce the samwe amino acids in our body.
--... 阅读全帖 |
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C*I
发帖数: 4736 | 25 相比较而言，石正丽的《Bat origin of human coronaviruses/蝙蝠是人类冠状病毒的
来源》是扎扎实实的抓蝙蝠，解剖蝙蝠，分离病毒，对比DNA,RNA后得出得结论。
请lobbock读一读，说一说，走两步！
http://virologyj.biomedcentral.com/articles/10.1186/s12985-015-0422-1
Review
Open Access
Published: 22 December 2015
Bat origin of human coronaviruses
Ben Hu, Xingyi Ge, Lin-Fa Wang & Zhengli Shi
Virology Journal volume 12, Article number: 221 (2015) Cite this article
Abstract
Bats have been recognized as the natural reservoirs of a large variety of
viruses. Special attention has ... 阅读全帖 |
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w******e
发帖数: 953 | 26 How safe does transgenic food need to be?
Laura DeFrancesco1
Journal name:
Nature Biotechnology
Volume:
31,
Pages:
794–802
Year published:
(2013)
DOI:
doi:10.1038/nbt.2686
Published online 10 September 2013 Corrected online 26 September 2013
Article tools
PDF
How safe does transgenic food need to be?
Download as PDF (812 KB) View interactive PDF in ReadCube
Citation
Reprints
Rights & permissions
Article metrics
Disputes over how to assess a foodstuff's safety continue to play into
public fears a... 阅读全帖 |
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a**********e
发帖数: 418 | 27 Please do some research and find out what amino acids were made in the
experiment and comment again.
---are you considering these amino acids are the same as what are in our
body? Otherwise we would have a (L,S) body instead of L body
well I can assure you the lab symmetric and assymetric synthesis of amino
acid is well known. One of them is strecker synthesis I happened to work on
one of the project. I can purify the amino acid to any degree of purity
you want by different means. So please b... 阅读全帖 |
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R******d
发帖数: 5739 | 28 Please do some research and find out what amino acids were made in the
experiment and comment again.
---are you considering these amino acids are the same as what are in our
body? Otherwise we would have a (L,S) body instead of L body
--------------------------------------
please be specific on what you are refering to. if you are refering to the
stereogenicity, then you are correct. chirality is one of the unsolved
mystery. However, it is only unsolved at this time. It has nothing to do
wit... 阅读全帖 |
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u*********s
发帖数: 2077 | 29 http://www.active.com/nutrition/Articles/Get_enough_protein_aft
After exercise, it starts. The body begins the process of recovery, adapting
and preparing for the next challenge.
There are two basic types of recovery. The first is the restoration of fuel
supplies--the carbohydrates and fats that supply energy to the working
muscle.
The second is adaptation, in which the structure and metabolic processes of
the muscles are rebuilt and reinforced to be stronger and more efficient.
Different types ... 阅读全帖 |
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w*******y
发帖数: 60932 | 30 I haven't used this stuff yet myself but just ordered 5 based upon
recommendations and the sale price on the 35 servings tub. Also, the 9.2
rating on BB.com seems pretty reassuring.
Keep in mind the website gives away a selection of free gifts for orders
over $75, $100, and $150 price points. You will see this clearly as you
order, better gifts the more you spend obviously.
Shipping seems to be between 7-10$ for me depending on what I had in my cart
, but the shipping went up very little with ... 阅读全帖 |
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x****6
发帖数: 4339 | 31
The occurrence of concentrated pneumonia cases in Wuhan city, Hubei province
of China was first reported on December 30, 2019 by the Wuhan Municipal
Health Commission (WHO, 2020). The pneumonia cases were found to be linked
to a large seafood and animal market in Wuhan, and measures for sanitation
and disinfection were taken swiftly by the local government agency. The
Centers for Disease Control and Prevention (CDC) and Chinese health
authorities later determined and announced that a novel coro... 阅读全帖 |
|
k****o
发帖数: 728 | 32 一个Proteomics/Genomics 方面的open access journal，我正在handle一个稿子，需
要寻找几个reviewers。文章title/abstract如下。杂志也不是多好，但也能派上用场
。感兴趣的可站内联系，我需要尽快发出邀请，已经拖了几天了。
请给出name, institute, position, email, expertise, and representative
publications related to this manuscript.
Article Title : Classification of non-animals and invertebrates based on
amino acid composition of complete mitochondrial genomes
Abstract :
Amino acid compositions were predicted from data sets of 47 non-animal and
58 invertebrate animal complete mit... 阅读全帖 |
|
n***s
发帖数: 10056 | 33 From:
http://answers.google.com/answers/threadview/id/724604.html
Note: excessively, dramatic increase....
It is possible that a diet excessively high in protein can
cause elevated liver enzymes.
?High-protein diets are generally well tolerated by healthy adults.
But a dramatic increase in protein-rich foods may be
dangerous for people with liver or kidney disease. This is because they lack
the ability to get rid of the waste products left from protein metabolis... 阅读全帖 |
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N****O
发帖数: 4202 | 34 补充一些：
How Your Brain Makes Serotonin
Serotonin is considered a monoamine neurotransmitter, which means it is made
by one amino acid. Amino acids are the building blocks of protein. There
are two other monoamine neurotransmitters: dopamine and norepinepherine.
In order to produce serotonin, your body needs the amino acid tryptophan,
which is an essential amino acid. Essential amino acids are not made by your
body, so you must get them from your food. To produce adequate amounts of
serotonin it is |
|
m**e
发帖数: 857 | 35 can you believe of it? Just 3 amino acid.
Regulatory evolution through divergence of a phosphoswitch in the
transcription factor CEBPB
Lynch VJ, May G, Wagner GP.
Nature. 2011 Nov 13;480(7377):383-6. doi: 10.1038/nature10595.
Abstract
There is an emerging consensus that gene regulation evolves through changes
in cis-regulatory elements and transcription factors. Although it is clear
how nucleotide substitutions in cis-regulatory elements affect gene
expression, it is not clear how amino-acid sub... 阅读全帖 |
|
k****o
发帖数: 728 | 36 一个Proteomics/Genomics 方面的open access journal，我正在handle一个稿子，需
要寻找几个reviewers。文章title/abstract如下。杂志也不是多好，但也能派上用场
。感兴趣的可站内联系，我需要尽快发出邀请，已经拖了几天了。
请给出name, institute, position, email, expertise, and representative
publications related to this manuscript.
Article Title : Classification of non-animals and invertebrates based on
amino acid composition of complete mitochondrial genomes
Abstract :
Amino acid compositions were predicted from data sets of 47 non-animal and
58 invertebrate animal complete mit... 阅读全帖 |
|
|
j*****g
发帖数: 7513 | 38 学过大学的生化课，就知道胶原蛋白和一般蛋白质的结构是很不一样的。下面的内容摘
自wikipedia.之所以要坚持，是因为皮肤的新陈代谢差不多120天才能换一层新的。
Collagen is composed of a triple helix, which generally consists of two
identical chains (α1) and an additional chain that differs slightly in its
chemical composition (α2).[25] The amino acid composition of collagen is
atypical for proteins, particularly with respect to its high hydroxyproline
content. The most common motifs in the amino acid sequence of collagen are
glycine-proline-X and glycine-X-hydroxyproline... 阅读全帖 |
|
B******1
发帖数: 9094 | 39 Wrong impression. Let's put it this way: we all know that there are only 20
amino acids in nature. Long time ago the amino acid sequence of
chymotrypsinogen-B was determined to have 245 amino acid residues. If one
wants to build all the possible variations of chymotrypsinogen-B using the
number of amino acid building blocks (20) available, the numberof possible
sequence combinations (20exp(245) or 5.65 x 10exp(318)) could easily be
deduced.
It turned out that the estimated quantity of matter i... 阅读全帖 |
|
c******i
发帖数: 2427 | 40 1. Personal experience has nothing to do with science.
2. All the proteins are broken down by the special enzymes (protease )in
stomach and intestine into single amino acids. These amino acids then are
transported in the blood into the tissue like muscle. Muscle cells take up
the amino acids through special transporters on muscle cells' membrane. Then
amino acids move into the cell. Then they are incorporated into protein to
build stronger muscle cells after you lift heavy weight. This is the ba |
|
F***I
发帖数: 4170 | 41 Monosodium glutamate
From Wikipedia, the free encyclopedia
Jump to: navigation, search
This article is about the chemical compound. For its use in food, see
glutamic acid (flavor). For glutamic acid in general, see glutamic acid.
Monosodium glutamate
Chemical composition of monosodium glutamate
Structure of monosodium glutamate
Crystalline monosodium glutamate
IUPAC name[hide]
Sodium 2-Aminopentanedioate
Identifiers
CAS number 142-47-2 Yes
PubChem 85314
ChemSpider 76943 Yes
UNII ... 阅读全帖 |
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D*****t
发帖数: 1303 | 42 好像木看到跟蛋有关的成分啊？blue buffalo的就是这个wilderness的好，其他的都不太好
http://bluebuffalo.com/products/cats/wilderness-cat-dry.shtml
Ingredients
Deboned Chicken, Chicken Meal, Turkey Meal, Potato Starch, Fish Meal(natural source of Omega 3 Fatty Acids), Chicken Fat (naturally preserved with Mixed Tocopherols), Peas, Whole Potatoes, Flaxseed (natural source of Omega 3 and 6 Fatty Acids), Natural Chicken Flavor, Alfalfa Meal, Whole Carrots, Whole Sweet Potatoes, Cranberries, Blueberries, Barley Grass, Dried Parsley,... 阅读全帖 |
|
c*******t
发帖数: 168 | 43 我们家吃的是这个spa select，把成分找出来了，这款果然是有鸡蛋的。
为什么说只有wildness的好其他都不好阿，我看配料都差不多似得阿。
http://www.petco.com/product/102979/Spa-Select-Adult-Chicken-and-Brown-Rice-Recipe-Cat-
Food.aspx?CoreCat=CatFFB_BlueBuffalo
不太好
natural source of Omega 3 Fatty
Acids), Chicken Fat (naturally preserved with Mixed Tocopherols), Peas,
Whole Potatoes, Flaxseed (natural
source of Omega 3 and 6 Fatty Acids), Natural Chicken Flavor, Alfalfa Meal,
Whole Carrots, Whole Sweet
Potatoes, Cranberries, Blueberries, Barley Grass, Dried... 阅读全帖 |
|
p********f
发帖数: 5148 | 44 有人买过这个么？ingredients 非常好的样子。
Deboned Chicken, Deboned Turkey, Chicken Meal, Turkey Meal, Menhaden Fish
Meal (natural source of Omega 3 Fatty Acids), Whole Ground Brown Rice, Whole
Ground Barley, Oatmeal, Peas, Chicken Fat (naturally preserved with Mixed
Tocopherols), Dried Egg, Natural Chicken Flavor, Whole Potatoes, Fish Oil (
natural source of Omega 3 Fatty Acids), Flaxseed (natural source of Omega 3
and 6 Fatty Acids), Whole Carrots, Whole Sweet Potatoes, Cranberries,
Blueberries, Barley Grass... 阅读全帖 |
|
p********f
发帖数: 5148 | 45 Have you tried Merrick turducken?
Crude Protein (Min.) 10.00%
Crude Fat (Min.) 3.00%
Crude Fiber (Max.) 1.5%
Moisture (Max.) 78.00%
Ash (Max.) 1.80%
Magnesium (Max.) .025%
Taurine (Min.) .08%
Ingredients:
Turkey, Chicken, Chicken Broth, Duck, Fresh Sweet Potatoes, Fresh Carrots,
Fresh Snow Peas, Fresh Golden Delicious Apples, Potato Starch-Modified,
Dried Cranberry, Olive Oil, Flax Seed Oil (For Omega -3), Cassia Gum,
Carra... 阅读全帖 |
|
c**c
发帖数: 2593 | 46 嗯，好像作者实际上实现起来比四个字母要复杂，用的是氨基酸序列。作者特地做了如
下说明：
To keep to 'the spirit of the genome', I did the conversion, not from the
bases (adenine, cytosine, thymine, guanine), but from the 'codons'--the sets
of three bases that code for an amino acid. There are 64 (4X4X4) possible
codons and only 20 amino acids. Amino acids then, are represented by a
variable number of codons--from six down to one. I associated those amino
acids with the highest number of codon representations with the most common
notes of t... 阅读全帖 |
|
a*******u
发帖数: 19 | 47 N-terminal fragment C-terminal fragment
——————————————— ————————————————
方案1：
引物设计如下
PrimerN_For: A$$$$$$$$$$$$$$
PrimerN_Rev: B**********
PrimerC_For: B&&&&&&&&&&&
PrimerC_Rev: C@@@@@@@@
A,B,C分别表示 不同的内切酶。
用分别将两端PCR下来依次插入到载体中，即N端以空载体为载体插入，然后将C端以N端
插入完毕的质粒做载体插入。这样的问题是多了一个B的内切酶序列，多2个aminos。
deletion mutants允许多2个外源的aminos吗？
方案2：
引物设计如下：
PrimerN_For: A$$$$$$$$$$$$$$
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发帖数: 1481 | 48 Amino Acids, Peptides and Proteins in Organic Chemistry: Modified Amino
Acids, Organocatalysis and Enzyme, Volume 2
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Amino Acids, Peptides and Proteins in Organic Chemistry: Building Blocks,
Catalysis and Coupling Chemistry, Volume 3
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Amino Acids, Peptides and Proteins in Organic Chemistry: Protection
Reactions, Medicinal Chemistry, Combinatorial Synthesis, Volume 4
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发帖数: 3799 | 49 http://www.beginnertriathlete.com/cms/article-detail.asp?articl
Confused about how much protein you need, when you need it, and the best
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