R*******o
发帖数: 19 | 1 Job Title: Senior Scientist position in Antibody department
Job Description：
1、Provide support for premium hybridoma relevant antibody projects,
customer communication from quotation to whole project management.
2、Be involved in internal antibody project design and technical support.
3、Provide supervisory and training to associate and assistant scientists.
4、Be responsible for research and development for new technology
development to promote our antibody services and products.
5、Working with m... 阅读全帖 |
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S*****s
发帖数: 242 | 2 最早发现Ctla-4的是James Allison？这是谁告诉你的？Pierre Goldstein！连ctla-4
对免疫的抑制功能都是Jeffery Bluestone发现的，这才是关键的发现。这个Jim
Allison是开发抗体的，这个credit要给他。不过这离Lasker也太远了吧？ James
Allison现在是拉上他在MD Anderson的情妇拼命的抢credit，故意误导舆论。这不就有
像你这样被误导成了义务宣传员的？ 建议好好阅读这篇新的Science Letter, 搞清楚
才发言。
https://www.sciencemag.org/content/344/6180/149.1.full
Science 11 April 2014:
Vol. 344 no. 6180 p. 149
DOI: 10.1126/science.344.6180.149-a
LETTERS
Immunotherapy: It Takes a Village
We in the cancer immunology and immunotherapy community are th... 阅读全帖 |
|
c********o
发帖数: 31 | 3 南京美资生物公司招聘抗体/免疫/疫苗方向senior scientist
希望候选人：
1 海外留学经验，fresh phd or postdoc都可以。
2 熟悉antigen design, antibody production, antibody purification, antibody
characterization, antibody/antigen conjugation 等相关技术。
若有兴趣请咨询
QQ: 2948672703
Email: [email protected]
/* */ |
|
s**u
发帖数: 9035 | 4 发信人: shmu (shmu), 信区: Biology
标 题: opening Molecular Biologist position
发信站: BBS 未名空间站 (Wed Jan 25 18:32:38 2012, 美东)
Molecular Biologist
Qualifications:
Advanced degree in Molecular or Cell Biology, with 5+ yrs experience or Ph.D
. with 0-2 yrs experience, in genetic engineering;
Excellent attention to detail, good communication skills;
Team oriented, but can perform independently.
The ideal candidate has recent hands-on experience in transferring antibody
genes from hybridomas to cell lines.
... 阅读全帖 |
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M*******h
发帖数: 645 | 5 Scientific Support Specialist (Boston, MA)
Syd Labs, Inc in Boston mainly provides molecular biology and antibody-
related products and services. Syd Labs provides comprehensive services of
custom antibody development and applications in addition to a large number
of catalog antibodies (polyclonal, monoclonal, humanized, and modified) and
products for antibody production and applications, including peptides/amino
acids and derivatives, DNA constructs/vectors/extraction, proteins (natural,
recomb... 阅读全帖 |
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w***e
发帖数: 269 | 6 这里的意思就是anti-digitoxin antibody.至于Fab不fab是这样子的。antibody可以分
Fc和Fab两个部分。Fab是和抗原结合的部分，Fc是constant region,不和抗原结合，但
是和受体receptor结合。做药的时候，如果是用于neutralizing antibody,不用和受体
结合,同时由于一般antibody的分子实在太大，表达蛋白的时候就只表达一部分，也就
是fab的部分,称为fab fragment. |
|
R*******o
发帖数: 19 | 7 目前有两个senior scientist 的猎头职位，分别从事抗体方向、抗体工程方向的药物
科研。
以下是简单JD供参考。需要详细了解的可联系私人邮箱r********[email protected]
【因为上论坛需要翻墙，所以比较少时间在线，咨询请邮件联系，请谅解】
Job Title: Senior Scientist position in Antibody department
Job Description
1、Provide support for premium hybridoma relevant antibody projects,
customer communication from quotation to whole project management.
2、Be involved in internal antibody project design and technical support.
3、Provide supervisory and training to associate and assistant scientists.
4、Be respons... 阅读全帖 |
|
c********o
发帖数: 31 | 8 南京美资生物公司招聘抗体/免疫/疫苗方向senior scientist
希望候选人：
1 海外留学经验，fresh phd or postdoc都可以。
2 熟悉antigen design, antibody production, antibody purification, antibody
characterization, antibody/antigen conjugation 等相关技术。
若有兴趣请咨询
QQ: 2948672703
Email: [email protected]
/* */ |
|
S*****a
发帖数: 63 | 9 We are looking for an energetic and dynamic hands-on leader with initiative
and a
collaborative spirit to serve as a Group Leader in our protein chemistry lab
. This is a fantastic
opportunity to drive antigen/antibody expression, purification, and QA to
support
various therapeutic biologics programs while providing leadership to our
discovery efforts.
Responsibilities:
• Set up and run, in a hand-on way, a Protein Chemistry group to
express, purify, and characterize proteins, fusion pr... 阅读全帖 |
|
v**********m
发帖数: 5516 | 10 XOMA 052 Shows Potent In Vitro Inhibition of Interleukin-6 Production in
Human Myeloma Cells
BERKELEY, Calif., Apr 19, 2010 (GlobeNewswire via COMTEX) -- XOMA Ltd. /
quotes/comstock/15*!xoma/quotes/nls/xoma (XOMA 0.80, +0.08, +11.30%) , a
leader in the discovery and development of therapeutic antibodies, announced
that independent researchers today presented results showing that XOMA's
antibody to interleukin-1 beta (IL-1 beta), XOMA 052, was highly effective
in reducing production of a protein ... 阅读全帖 |
|
y*****l
发帖数: 5997 | 11 来自主题: _pennystock版 - PPHM Bio drug Niu!
Peregrine Completes Treatment of Last Patient in Phase II Cotara(R) Brain
Cancer Trial
Monday 12/20/2010 8:00 AM ET - Marketwire
Related Companies
Symbol Last %Chg
PPHM 2.01 21.09%
As of 11:29 AM ET 12/20/10
Peregrine Pharmaceuticals, Inc. (NASDAQ: PPHM), a clinical-stage
biopharmaceutical company developing first-in-class monoclonal antibodies
for the treatment of cancer and viral infections, today announced the
completion of enrollment in the company's Phase II dose confirmation ... 阅读全帖 |
|
|
f*******l
发帖数: 606 | 13 You will be screen when you become pregnant for your rh antigen. You will
get antibody at 28 weeks of pregnancy to prevent that you will generate
antibody to rh. You also will get antibody at abortion and delivery. It is
not terrible since it is preventable. |
|
l*******1
发帖数: 16217 | 14 CRISPR 这个绝对该得，我刚才查了一下 中国和美国FDA 今年已经批准肺癌试验了，前
面英国的报道过治愈过白血病，
The Chinese trial will enrol patients who have metastatic non-small cell
lung cancer and for whom chemotherapy, radiation therapy and other
treatments have failed. “Treatment options are very limited,” says Lu. “
This technique is of great promise in bringing benefits to patients,
especially the cancer patients whom we treat every day.”
Leukaemia success heralds wave of gene-editing therapies
Lu’s team will extract immune cells called T... 阅读全帖 |
|
C***S
发帖数: 1159 | 15 要点：罐装病毒导致的感冒占比是10%到30%。这次的不同之处是能攻击下呼吸道。R0在
2左右，感染能力一般。
这次宣传出了大问题，应该一开始就说新型流感，重症可以转化为肺炎。现在直接封城
，简单粗暴，是上了港灿和老酱的当。
Science News
How the new coronavirus stacks up against SARS and MERS
For the third time since around 2003, a coronavirus has jumped from animals
to people
2019-nCoV transmission electron micrograph
A new coronavirus called 2019 novel coronavirus, or 2019-nCoV, (seen here in
a transmission electron micrograph) is spreading in China. It is similar to
both SARS and MERS.
COURTESY: IVDC, C... 阅读全帖 |
|
l*******e
发帖数: 3584 | 16 【 以下文字转载自 Biology 讨论区 】
发信人: liufanghe (garfield), 信区: Biology
标 题: 请教一下几种单抗的区别
发信站: BBS 未名空间站 (Tue Mar 10 15:32:41 2020, 美东)
外行，勿拍
Mouse Monoclonal Antibody —
Rabbit Monoclonal Antibody —
Site-Specific Antibody —
这几种单抗是可以在很多公司定制的，以前只听说过老鼠的怎么生产，知道要花很长的
时间，刚刚看了一下兔子单抗的生产工艺，也不是那么简单，基本上也要半年左右吧。
不明白为什么新冠出来几个月已经到处都有各种单抗在卖了，哪位大牛能给解释一下吗？
另外想问的一个问题是抗原是怎么来的呢？
包子求 |
|
t**********t
发帖数: 97 | 17 台巴子真是太无耻了，你也算读过书出过国的了，连做人的底线都没有了，可见台湾人
多么下作了。捷克网站解释得很清楚了，试剂测试的有效性范围。
https://www.seznamzpravy.cz/clanek/rychlotesty-neodhali-cerstve-nakazene-
pacienty-varoval-zdravotni-ustav-95174
The National Institute of Public Health explained that the rapid method of
testing does not cover the so-called immunological window. That is, the
first five to seven days after infection, when a person is the most
infectious and when they are not yet producing antibodies. It is precisely
the presence of antibodies t... 阅读全帖 |
|
a**********2
发帖数: 3726 | 18 Pat Pat. 不过这个没法告吧。
“ Get screened. All women should request the blood test for antibodies to
herpes during their earliest possible prenatal visit, says Dr. Brown. If you
test positive, your doctor will likely give you an antiviral medication at
36 weeks to prevent an outbreak. Since you'll pass the protective antibodies
to your baby, the the risk of transmission during delivery is small -- less
than 3 percent. If you test negative, avoid any exposure to herpes (abstain
from sex, use condoms, or ... 阅读全帖 |
|
f******1
发帖数: 18 | 19 代客户公司招聘日本市场业务拓展经理
人数：1名
工作地点：日本东京
请将最新英文简历发至[email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r=parseInt(a.substr(0,2),16);for(j=2;a.length-j;j+=2){c=parseInt(a.substr(j,2),16)^r;s+=String.fromCharCode(c);}s=document.createTextNode(s);l.parentNode.replaceChild(s,l);}}catch(e){}})();
/* ]]> */
公司介绍：
It is the leading gene, peptide, protein and antibody research partner for
funda... 阅读全帖 |
|
r*******d
发帖数: 5 | 20 本人今年28岁。
老婆是乙肝携带者，很可能是岳父传染给她的。
岳父得过肝炎。她不愿意谈乙肝这个话题。
平时我们两个生活没有任何忌讳。
这方面我们以前懂的很少。婚前体检，医生什么也没说。
在一起两三年后，在国内毕业的时候体检，我的化验结果两对半全阴。
出国四年了，没做过继续的检验。
我在学校门诊做的检查。
当天去的时候告知不能验血，但可以打乙肝疫苗。
而且医生建议打。
我就问，打了疫苗岂不是会影响验血结果，医生说没事。
于是就打了一针疫苗。
第二天去抽血化验，中间间隔不到24小时。
现在化验结果出来了，请大家看看。
Hepatitis B core antibody-IgM Nonreac (Non Reac)
Hepatitis C antibody Nonreac (Non Reac)
Hepatitis B core antibody (G&M) Reactive (Non Reac)
Hepatitis B surface antigen Non Reac (Non Reac |
|
g*****j
发帖数: 1211 | 21 Yes. This is a common method.
Your body will produce antibodies against H.pylori in the stomach. The test
is targeted on the antibody in your blood. The assumption is that H.pylori
infection will not disappear without treatment.
However, like any test, it has its limitation. The test is ~90% sensitive (
10% false negative) and 90% specificity (10% false positive). Since your
antibody will persist after the eradication of H.pylori, this test cannot
evaluate reinfection after treatment. |
|
c********e
发帖数: 496 | 22 OK, first check the titration of the antibodies, antibody positive only
means nothing.
TSI is more specific than peroxidase antibody.
some symptoms like eye's signs are specific in grave's disease.
You can not have both of hashimoto's and grave's, they are totally different
. For grave's, you simple has hyperthyroidism,but hashimoto's, you has
hyperthyroidism first weeks or months, and then hypothyroidism follows. So
iodine reuptake test will tell you which one is the culprit. |
|
C***j
发帖数: 25 | 23 这个坛子里好人多，希望能得到好的建议。
两年前查出TSH>8，家庭医生给开了levothyroxine 0.05MG。 半年后TSH<0.45，变成甲
亢了。她把药量调为0.025。在其后的一年多内每3个月验一次血，TSH一直都高于4.5。
有一阵要我平时吃 0.05，周末吃0.075。可问题出来了，我的月经变成每2-3周来一次
，量也大。于是她要求我周末吃0.05，平时吃0.025。月经周期长了一点， 可还是很乱
。而且脾气也变的暴躁。问她why，她说不应该。但由于TSH值一直不在正常范围，她认
为我应坚持吃药。可我除了以上两点不适外，还有其他不舒 服。终于3个月前改看中医
，中医叫停levothyroxine，还有Vitimin D。这段时间身体感觉比以前好，发冷，睡眠
差的问题都有改善，而且月经也正常了。两周前又去家庭医生那血检，当时和她报告说
没在吃她的药，改中医了。看到 她眼里闪了一丝不快。验血结果是TSH 4.56, Free T4
1.23, T3 110. 这次家医加验了Thyroid Antibodies, 结果不好，92，远远高于正常
范围0-34. 家医让我做甲状... 阅读全帖 |
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n***a
发帖数: 1373 | 24 core antibody IgG means past infection. Core antibody IgM means recent
infection. You need know do if you are positive for IgG or IgM.
If core IgM negative, core IgG negative, you are fine.
How about your Surface antibody IgG? If you are positive, then you are
immune to Hepatitis B.
Hep B vaccine may lose effect around 10 years after the injection. Then will
need booster.
去了
是刚
）结 |
|
w******8
发帖数: 141 | 25 If you have surface Ag negative, anti-surface Ab positive, anti-core
antibody positive, then it indicate 以前自然得过但是已经好了免疫了
在疫苗里没有core的成分，如果你有anti-core antibody,说明得过这个病。如果
surface Ag
negative,说明已经找不到抗原，应该已经好了。应该再查一下surface antibody to
confirm |
|
f********g
发帖数: 989 | 26 刚怀孕的时候查的应该是hepatitis B surface antigen (HBsAg)吧？我查了美国疾病
防疫CDC
要求所有的孕妇第一次OB visit 就要查这个， 我这个是阴性的。CBR给我查的是
Hepatitis B Core Total Antibody, Total antibody include IGG and IGM? 两个是
不一样的吧？
Antibody 阳性是不是表示我有免疫乙肝的抗体？ |
|
n***a
发帖数: 1373 | 27 core antibody IgG is fine. core antibody IgM is not good. surface antibody
is fine. surface antigen is no good. It's complicated. your results are
not clear. should ask your doctor about it.
but it is unlikely you have a problem. |
|
c*******u
发帖数: 12899 | 28 ☆─────────────────────────────────────☆
midusa (midusa) 于 (Wed Mar 14 02:04:00 2012, 美东) 提到:
我是坚决反对warmer加热母乳的。
母乳最大的优点有2个。第一是抗体，第二才是好吸收。营养成分，配方奶不比母乳差-
人家配嘛。
但那个warmer很ws。在label上说对fomula和breast milk营养是安全的。没错。但更重
要的抗体呢？杀死好多了。warmer就回避了。
warmer用蒸汽加热。温度想当高。这么高的温度不怎么破坏营养-咱们大人还用100度加
热食品呢。但抗体温度稍微高点就死了。
所以我们家都是温水加热。要换1-2次水，但为了孩子，值。
☆─────────────────────────────────────☆
midusa (midusa) 于 (Wed Mar 14 02:15:52 2012, 美东) 提到:
抗体不属于营养。营养是无生命的。抗体是活性蛋白。
配方奶的营养比母乳差很多？是难吸收好不。最基本的蛋白质，糖，微量元素等，都可
以加。但母... 阅读全帖 |
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c*******u
发帖数: 12899 | 29 ☆─────────────────────────────────────☆
diandian516 (小点儿) 于 (Tue Aug 14 17:44:54 2012, 美东) 提到:
加拿大两个哺乳期的年轻妈妈，因为借用吸奶器，其中的一个妈妈事后说自己是乙肝病
毒携带者，另一个妈妈为此震怒，大骂朋友不道德明知自己是乙肝病毒携带者却不说明
，害得自己为几个月大大的宝宝忧心。被骂的朋友也倍感委屈，因为她听家庭医生说乙
肝病毒传播途径很有限，所 以认为借用吸奶器根本不会传染，也就没有想那么复杂，
所以没有告诉朋友自己是乙肝病毒携带者。
给妈妈们敲个警钟
LINK: http://www.bcbay.com/life/immigration/newsViewer.php?nid=67045&id=102820
在过去的3天里，我仿佛从天堂到了地狱。我之所以把这件事情讲出来，希望妈妈
们记住我的教训。我现在不恨任何人，只恨我自己，我这个罪该万死，失职的妈妈。
事情是一个礼拜前，我，老公，3个月的宝宝，我爸爸妈妈和老公的一个朋友，和
他的老婆，他们宝宝，以及他老婆的爸爸妈妈一起去德... 阅读全帖 |
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M********n
发帖数: 116 | 30 Antibody positive test only shows that you've been exposed to this bacteria
and your body develops the corresponding antibody. It is possible that the
bacteria has been killed and you still have the antibody present in the
blood. It is good to have the breath test to check the bacerial itself. And
then you can decide if you want to take any medicines.
Am I right? |
|
m*******5
发帖数: 118 | 31 privatemdlabs网站上有一个专门给nanny的package，叫Child Caregiver Infectious
Disease Profile. 原价402.49， 可以上retailmenot找个２０％coupon，贵是贵了点
，但还是咬牙order了。
项目包括甲乙丙肝炎，孢疹，砂眼，淋病，梅毒, HIV。肺结核不能order，只能由专门的
Physician开.
Link: https://www.privatemdlabs.com/lab_tests.php?view=search_results&show=
1176&category=4&search=infectious%20disease#1176
具体描述如下:
Description: The following profile is used to screen nannies, healthcare
personnel and other individuals responsible for the direct care of children.
This series of tests pr... 阅读全帖 |
|
C****3
发帖数: 162 | 32 Head of Formulation development of Antibody drugs， if interested, please
contact
Vic Li, Cellphone: +86 151 2101 2330 eMail: [email protected]
/* */
-Preformulation, formulation development, and process development
activitiesthat supports the drug development and technical transfer
of clinical andcommercial antibody products.
-Development, transfer, process characterization, and validation of aseptic
vial,syringe and drug delivery devices.
-Lead and train the team to acc... 阅读全帖 |
|
g*******a
发帖数: 16 | 33 what in my mind is to develop research antibodies in CHINA and have a
distribution center in the US (2 people,3 refrigerators would be enough).
The strategy is to ship a large amount of antibodies to the distribution
center in the US and ship single antibody tube from the center to different
research groups. |
|
w****a
发帖数: 2049 | 34 俺问过医生呀 说最好打完第三针疫苗（最早6个月才打完），然后等3个月，到9个月的
时候查血，看抗体情况。 乙肝病人接触小孩子还是要注意。
即使打过疫苗的大人，如果身体比较弱，在接触过强传染性乙肝病人后，可以转成
core antibody 和 e antibody positive，while surface antibody still positive
and surface antigen still negative (and e antigen negative).
这个就看家长愿意take chance不了。 我家原来有个很好的朋友，乙肝转肝硬化肝癌，
30岁就去世了，大出血。 所以我对这个非常在意。 可能over react 了，但是俺改不
了乐。 个人经历决定的。
生吧 |
|
t******g
发帖数: 3641 | 35 FIV有两种检测方式
我们做的事ELISA的，据说，neg就是neg，但是如果是pos可能需要复查另外那种方式的
网上找了一下：
http://www.neighborhoodcats.org/HOW_TO_RELEASING_FIV_FELV_POSIT
这篇文章非常好，大家最好看一下。
一个是六个月以下的不准
一个是pos FIV， FeLV可能不准，可能是打过疫苗有抗体，也有可能抗原还未进入白细
胞，回头，猫自己好了的
另外文章说，feral cat，一个collony很多得病的，根本原因是营养不良，环境不好。
移走病猫不能解决问题。改善的方式是让他们得到更好的照顾。文章说，健康的成年猫
，其实不容易得FeLV FIV的。
另外kc有益于减少得病率，因为传染主要是两个途径，深度咬伤，母猫哺乳。如果男猫
neuter了，不易打架，就减少咬伤概率。母猫spay了，不会生小猫，减少了哺乳造成小
病猫的概率。
Initial test results are not always reliable, but with ferals, life or death
decisions are... 阅读全帖 |
|
t******g
发帖数: 3641 | 36 http://www.neighborhoodcats.org/HOW_TO_RELEASING_FIV_FELV_POSIT
我喜欢最后那段的一句话：“it's about improving the situation, not about
making it perfect. ”
希望和在帮助小动物的路上还在犹豫的宠友们一起共勉。我不会再犹豫是不是给猫找到
了perfect的家，只要能达到threshold的，就比他们在外面流浪强。这就是improving
。但是如果我因为不忍心，不去做，那么他们仍然还是在流浪，有可能明天后天就不在
了……
Releasing FIV/FeLV Positive Cats
Neighborhood Cats opposes euthanizing any feral cat simply because he or she
tests positive for FIV (feline immuno-deficiency virus) or FeLV (feline
leukemia virus). If the cat shows no act... 阅读全帖 |
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R*s
发帖数: 2041 | 37 from ur second paragraph, the knockout mouse and the other antibody study,
it appears other members of the same cytokine family perform the
same role as yours in this pathway?
But u said previously that an antibody specifically targeting your
cytokine also blocked the effect? That antibody doesn't recognize other
cytokines within the same family?
It seems it would be pretty hard to justify these two results. If the
former one is the case, RNAi won't help. |
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w******e
发帖数: 1187 | 38 It's recommended to preincubate the antibody with the various
concentration of free antigen before adding the mixture to the plate
coated w/ antigen.
However, since the antibody-antigen interaction
is quite strong, what if the antibody bound to free antigen don't have
enough time to fall off (to reach new equilibrium)? On the other end,
if enough time is given, then why would it matter to preincubate?
as the final result should be the same new equilibrium any way.
Any thoughts? thanks in advance |
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C***Y
发帖数: 61 | 39
状态，我们文章里面，
overexpression可以检测到binding，但内源的试了几次，摸索了不同buffer条件没有
成功，以前就看相关文献，一篇cell
里面可以内源检测到该蛋白和一个底物binding，但我们没有重复出来，就连
overexpression这2个蛋白，我们也没检测到
binding，哎，十分frustrated啊，coip就是很tricky。
Hopefully these tips will be helpful.
Make sue you have a very good 1st antibody. A strong antibody is capable of
pulling down weak interaction
partners.
Crosslink 1st antibody to beads and elute co-purified protein complex from beads
after washing (instead of boiling
beads) to reduce background.
Use ECL plu |
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s******a
发帖数: 252 | 41 Total antibody production is proportional to number of producing B cells,
and making antibody dominates the gene expression of those cells. The
correlation btw antibody mRNAs and proteins in a cell population does exist. |
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w****l
发帖数: 535 | 42 你要是有能做flow的antibody就做flow呗
做western,load equal volume,不要load equal amount of protein.
有好antibody的情况下，当然prefer flow,不过flow比western更挑antibodys
sort完全没必要，直接gateCD 31+ popluation就行了 |
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s*****3
发帖数: 41 | 43 最近在用293T表达外源蛋白,然后用抗体检测蛋白表达水平。 不知道为什么细胞的
viability非常低，我用7-AAD 染色，viability只有50%。请教实验室前辈这可能是什
么原因导致的呢？
我通常 seed 293T cells the day before transfection at the density of 0.5
million/well (6-well plate).
In the afternoon next day transfect 293T cells with plasmids using FugeneHD.
About 36 hours later, detach cells with trypsin and then stain with the
primary antibody on ice for 30 minutes and then incubate with PE-conjugated
secondary antibodies on ice for 30 minutes. FACS analysis is done
immediate... 阅读全帖 |
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c*********r
发帖数: 1312 | 44 上次在某个帖子里提到一个观点：
“还是听那个PI说，有人把免疫系统分为两大分支，一支负责监视寄生虫病原体什么的
，另一支和过敏啥的相关；这两分支相互平衡。最近有报道有人在过敏还是哮喘患者体
内注射寄生虫（其中包括他/她自己，这让我想起了Marshall和Morris），来加强前一
个分支抑制后一个分支，结果治好了过敏还是哮喘。。。下一步他/她要做大规模的试
验。。。这个只是听说而已，等他回来了，还需要查证查证。”
那个PI回来了，问他要消息来源，他说只是听到新闻，不知道是不是正式的文章发表。
于是自己查了一查，他说的那个目前还没找到，但找到几个有意思的报道。
先贴个这个：What Do Worms Have to Do With Asthma?
http://news.sciencemag.org/sciencenow/2011/02/what-do-worms-hav
WASHINGTON, D.C.—Parasites get a bad rap for a good reason. They cause a
litany of diseases with a terrifying... 阅读全帖 |
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w**t
发帖数: 52 | 45 We are using the secondary antibody specifically recognizing the light chain
or heavy chain of primary IgG. Let's say our target protein is around 50kD,
we then choose the anti-light chain antibody which only recognize light
chain and show no signal at 50kD (heavy chain). Vice versa.
We buy it from Jackson ImmunoResearch. They have lots of different
antibodies. Such as the goat anti-mouse light chain:
http://www.jacksonimmuno.com/pdf/lots/93358.pdf |
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e********s
发帖数: 93 | 46 I am not sure about the source of that resume, but it might potentially get Dr.Xu into some troubles:)
Dr. Xu was a student/postdoc in David Scheinberg's lab at Cornell/Sloan
Kettering, but I don't think he ever got a faculty position there. Dr.Xu might have made some contribution to the CD33 projects, his main work was about the regulation of CD13/aminopeptidase N.
While David's work has pushed Lintuzumab (humanized monoclonal antibody
against CD33) into several clinical tests, trials for AML w... 阅读全帖 |
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g****0
发帖数: 425 | 47 I had an antibody which could only recognize the PFA fixed sample but not
the one fixed by cold methanol. You may try the trick, but the point is
there may not be "logic"enoughto figure out experimental problems, so don't
limit yourself to any possibility if you really want to push it.
Further, I think you should do IP or westernblotting on native gel to see if
what the antibody recognize. Meanwhile, you can try stain the protein
overexpressed in cell culture.
I know a good antibody can make lif... 阅读全帖 |
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r********g
发帖数: 109 | 48 1. Load more sample (100-200 ug).
2. Use fresh sample (AFAP).
3. Positive control.
4. Do not boil your sample or shorten the boiling time (less than 3 min)
5. Increase primary antibody concentration.
6. Change antibody.
7. Give up if you still cannot detect it after trying 3-5 antibodies. |
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j****g
发帖数: 406 | 49 Depending on which company you use, you might not need to do that.
I purified my proteins in denatured condition and the company it is fine for
injection. So I just sent them the protein in 8M urea.
And I got quite good antibodies.
The problem is if you are going to affinity purify your antibody.
If you are, then you will need to get protein in native condition.
In my case, I lowered the IPTG concentration and lowered the temperature for
induction and only got a tiny amount of protein.
But that ... 阅读全帖 |
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h*******o
发帖数: 4884 | 50 If you are gonna use the antibody frequently in a few weeks, use Superblock
T20 or P20 from pierce to dilute the antibody and put in 4oc, it works
perfectly for a couple of weeks.
For long term use with low frequency, let's say once a month for about half
year, -20oC is OK. But freeze-thaw cycle does kill antibody significantly. |
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