t******k
发帖数: 599 | 1 想分离astrocyte出来直接做wb之类的生化实验，然后查来查去基本都是要培养来纯化
astrocyte，不过我觉得这样可能会改变最原始的蛋白状态，而且我们实验室从来没做过
astrocyte培养。看到macs他家的glast microbead sorting，想问问有没有其他简单一
些的分离方法？ |
|
f***4
发帖数: 886 | 2 GFAP不是，只是大家都这么用。
GFAP表达的细胞不一定是astrocyte，astrocyte不一定表达GFAP |
|
h*******o
发帖数: 4884 | 3 For primary neurons, we use neurobasal media +B 27. It is selective towards
neurons and glia usually cannot survive for long term in NBM+B27. With that
said, there is no pure neuronal culture. Anyway, cultured neurons do exhibit
some glial properties, such as glucose transporter expression, metabolic
activity, etc.
For embryonic glial culture, we use DMEM/F12 (1:1) +10% FBS. Because glial
cells are mitotic whereas neurons are post-mitotic, neurons will be easily
outnumbered by proliferative glia... 阅读全帖 |
|
f***4
发帖数: 886 | 4 astrocyte发育起源好像是不太清楚吧；主要是没有一个好的特异的标记分子。近年来
关于这个问题只是David Anderson发过篇cell。不过文章估计也是因为大牛实验室出来
的；本身没有解决什么问题，我认为。
这么重要的细胞，现在好像也搞得不清不楚地。
大家对这个方向怎么看啊？ |
|
p*****m
发帖数: 7030 | 5 GFAP不行么？我觉得看你的问题是astrocyte的起源 还是分类了 |
|
p*****m
发帖数: 7030 | 6 恩 我是外行随便问问。我有点不理解的是 搞neuron发育的比较直接 因为neuron总
是有分类的（不同的layer 不同的projection map，不同的neurotransmitter等等
） 这样也许可以用transcription code分类然后看他们分别怎么起源和分化的。但
是astrocytes（或者说glia）好像分类不像neuron那么清楚把 这样除了能study这
些细胞整体是哪来的还有啥别的能搞得没 |
|
a******g
发帖数: 129 | 7 astrocytes and glial cell are non-parenchymal cells in brain. They are the
other side of biology compared to various famous parenchymal cell types such
as neurons,beta cell, hepatocytes and so on. These non-parenchymal cells
provide unique niche to maintain the normal function of specific tissues and
organs. |
|
p*****m
发帖数: 7030 | 8 就是说起源都不清楚？我只有个很简单的印象就是glia也从neural progenitor ce
ll来 中间有七七八八的fate determination factor参与。呵呵 我基本对发育问题
的细节知道的是零。。而且好像oligodendrocyte比astrocyte的发育搞得清楚些？ |
|
h*****o
发帖数: 342 | 9 好像还有个蛋白标记glast也是用来标记 astrocyte |
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f***4
发帖数: 886 | 10 radial glia 也表达glast；一部分oligodendrocyte也表达。
现在glia的标记物也就oligodendrocyte比较清楚一些，现在还没有一个astrocyte特异
的标记物。看文章的时候总是用一个蛋白染色来说明，其实不一定的；没人深究 |
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n********k
发帖数: 2818 | 11 watch out Rowitch DH, last times he came here and gave a very very nice talk
. I think he is gearing up to
dissect some of those with genetic and lineage tracing tools on astrocytes,
which were very nice and
impressive... |
|
f***4
发帖数: 886 | 12 Rowitch是什么都作，哪个实验室都合作，什么结果都算自己的。一个业内绝顶之人的
样子。最近他关于astrocyte发育的文章已经连续被几家杂志据了，他说 |
|
e*******r
发帖数: 275 | 13 解剖打散之后出来的都是各种细胞的混合物吧, 怎么分离和处理才能拿到纯的神经元或
者纯的胶质细胞呢? 加arac是抑制astrocyte分裂的,不能杀死它们吧. 加不加BSA会不
会有不一样? |
|
b******9
发帖数: 425 | 14 做了个construct，转primary newborn astrocytes，用hygromycin来筛选stable
transfection，可是一直都没有筛出来，细胞全都死光光了。转染效率还算可以，是电
转，有30%左右。hygromycin浓度也不算高，50ug/ml。
hygromycin是用TK promoter表达的，不知道是不是因为promoter不行。试了别人的一
个puromycin construct，是pGK promoter，也基本上死光了。想问一问版上牛人有没
有经验可以传授。多谢多谢！
另外我是用CMV promoter来表达我要的基因，先转细胞，然后筛选stable line打到老
鼠上。听说CMV promoter在体内可能会被shut down，是不是也应该换掉呢？谢谢！ |
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h*******o
发帖数: 4884 | 15 既然有transgenic mouse strain了， 那就把colony 建立起来，然后原代培养
astrocytes和microglia就行了啊
这个不是adult neuron培养，容易多了
如果你不要adult的， embryonic的neuron也好养 |
|
z****2
发帖数: 2 | 16 One or Two Postdoctoral positions are available (starting from August of
2010) in the laboratory of Dr. Yongjie Yang, to study the broad neuron to
astrocyte interaction, in particular, the roles of neuron to astrocyte
signaling in astrocyte development, the mechanisms/pathways of astrocyte
dysfunction in neurodegenerative diseases, such as motor neuron disease,
epilepsy, and Alzheimer’s diseases. This represents a very exciting and
important but less studied area in neurobiology field. The lab e |
|
c****t
发帖数: 19049 | 17 ScienceDaily (July 9, 2011) — For the past decade, researchers have tried
to reprogram the identity of all kinds of cell types. Heart cells are one of
the most sought-after cells in regenerative medicine because researchers
anticipate that they may help to repair injured hearts by replacing lost
tissue. Now, researchers at the Perelman School of Medicine at the
University of Pennsylvania are the first to demonstrate the direct
conversion of a non-heart cell type into a heart cell by RNA transfer... 阅读全帖 |
|
z****2
发帖数: 2 | 18 One or Two Postdoctoral positions are available (starting from August of
2010) in the laboratory of Dr. Yongjie Yang, to study the broad neuron to
astrocyte interaction, in particular, the roles of neuron to astrocyte
signaling in astrocyte development, the mechanisms/pathways of astrocyte
dysfunction in neurodegenerative diseases, such as motor neuron disease,
epilepsy, and Alzheimer’s diseases. This represents a very exciting and
important but less studied area in neurobiology field. The lab e |
|
d*****r
发帖数: 2583 | 19 希望生物里面多一些说No的文章，不要搞得什么东西都跟什么东西相关。
Science 5 March 2010:
Vol. 327. no. 5970, pp. 1250 - 1254
Hippocampal Short- and Long-Term Plasticity Are Not Modulated by Astrocyte
Ca2+ Signaling
Cendra Agulhon,1,* Todd A. Fiacco,2 Ken D. McCarthy1
The concept that astrocytes release neuroactive molecules (gliotransmitters)
to affect synaptic transmission has been a paradigm shift in neuroscience
research over the past decade. This concept suggests that astrocytes,
together with pre- and postsynaptic neuronal |
|
h*******o
发帖数: 4884 | 20 门外汉一个，最近在做不同药物对NSC differentiation的影响
一个药很奇怪， 加进去以后， 然后换differentiation media （过程中保持药
物presence），最后fix染色， 用map2和GFAP染色看neuron／astrocyte的比例
其他组型态都正常，
这个药导致map2染色增强，但是我看型态又不象是neuron的型态，更接近于
astrocytes的型态。 GFAP染色和型态都正常。
所以问问看这个一般会是什么情况导致map2表达增高，但是细胞长的像astrocytes？ |
|
f**d
发帖数: 768 | 21 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
|
f*******3
发帖数: 122 | 22 RE: Applicant’s Name
To Whom It May Concern:
It gives me great pleasure to write this letter in support of Dr. XXX’s
research contributions to the field of ____________________.
I am Chair of Molecular Biology at the School of Biological Sciences at the
University of ____________________________. I have spent _______ years in
academic research, publishing approximately _________scientific publications
in peer-reviewed, internationally distributed journals, which have been
cited ________ tim... 阅读全帖 |
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B*******e
发帖数: 3882 | 23 astrocytosis:
" An abnormal increase in the number of astrocytes due to the des
truction of nearby neurons, typically because of hypoglycaemia or oxygen dep
rivation."
"Medicine. a proliferation of astrocytes owing to the destruction of nearby
neurons during an episode of hypoxia or hypoglycemia. " |
|
M****e
发帖数: 70 | 24 this Nature paper (2002 417:39) addressed the mechanisms that
control fate of neural stem cells. astrocytes have been
thought merely as supportive cells in the central nervous
system, however, this work has demonstrated that astrocytes
isolated from adult hippocampus, but not spinal chord,
promoted neurogenesis (i.e., neuron fate) of the adult neural
stem cells in cell culture. this study has highlighted the
effects of the environment on the specification of stem cells.
for stem cell research, m |
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C********4
发帖数: 308 | 25 假如下面的文章都是同一个人发表的。
请问在这个领域算是上等，还是中等，还是比较差的？
＝＝＝＝＝＝＝＝
1.
Metallic gold slows disease progression, reduces cell death and induces
astrogliosis while simultaneously increasing stem cell responses in an EAE
rat model of multiple sclerosis.
Pedersen DS, Fredericia PM, Pedersen MO, Stoltenberg M, Penkowa M, Danscher
G, Rungby J, Larsen A.
Histochem Cell Biol. 2012 Jul 22. [Epub ahead of print]
2.
Metallic silver fragments cause massive tissue loss in the mouse brain.
Locht LJ, Pedersen MØ, Markhol... 阅读全帖 |
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G*********y
发帖数: 100 | 26 glioblastoma是astrocytoma的一种。
astrocytoma就是从astrocyte来源的肿瘤，GFAP阳性。
成人的Glioblastoma multiforme (grade IV astrocytoma)
儿童的Pilocytic (low-grade) astrocytoma
是FA上需要掌握的两种astrocytoma，成人的恶性程度很高。
astrocyte就是一种Glial cells，所以你看GBM的名字就可以理解了。 |
|
e***o
发帖数: 344 | 27 首先感谢文献帮助!!!
是否可以这么说，大多数 glial cell 主要功能是supporting ，保护，修复neurons。
但是glial cell太多了，分类不清。就如interneuron一样，没有好的marker， cre-
line就无从下手一样。
但是越来越多的功能正在被挖掘，譬如分泌axon guidance 信号, 回路布线，
tripartite synapse，neurotransmitter releasing, clearance。。。。。
也有些文章开始提Glial cell plasticity，虽然有炒作概念之嫌，但也并非孔穴来风
。最近也读过几篇Glial cell和记忆的文章，好像就是通过营养等通路来影响的。
去年science一片很有意思的paper，分享一下，
呼吸-----co2 浓度----ph 改变--- astrocytes ca2+ dependent ATP release---
activate chemoreceptor neuron.
应该说neuron还是执行者，但是glial cell的作用应该是被underestim... 阅读全帖 |
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n***w
发帖数: 2405 | 28 This is a very interesting area to explore.
The contributions of glial cells (astrocytes, oligodendrocytes and microglia
) are more than what they originally were thought.
I have a particular interest in neuron-glia interactions and their outcomes
(neuroprotection in my case).
Also, gliovascular relationship should not be neglected neither since
astrocytes can form end-feet structures on endothelial cells which are
important for BBB integrity and nutrient supply. |
|
A*****a
发帖数: 52743 | 29 可以这么说。干细胞本应该分化成astrocyte或者glial cell的，但没有完成便出现癌
变。 |
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l******n
发帖数: 66 | 30 Barclay Morrison III, Biomedical Engineering is looking to fill a post-doc
opening in the biomedical engineering department It is funded for 3 years.
The main focus of the funded research project will be to test the
therapeutic efficacy of a novel combination of compounds with the ability to
modulate astrocyte-mediated glutamate homeostasis in the setting of
traumatic brain injury using our in vitro model (hippocampal slices).
Therapeutic efficacy will be quantified with a combination of biochem |
|
O**********5
发帖数: 3 | 31 Two positions for Postdoctoral Research Scientists
We are seeking highly qualified and motivated candidates who share a passion
for scientific discovery to join an interactive team.
Post #1: The molecular and cellular mechanisms controlling the balance of
immune response in the mouse brain.
Chronic neuroinflammation is a common feature of the ageing brain and a
variety of neurodegenerative disorders. However, the molecular and cellular
mechanisms underlying the regulation of innate immunity in t... 阅读全帖 |
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R********n
发帖数: 5904 | 32
那是人裴校长的聘礼吧，这之前混得很灭。
发信人: zhongguo2 (ee), 信区: ChinaNews
标 题: 谢欣 and 裴钢
发信站: BBS 未名空间站 (Tue Jun 21 15:37:17 2011, 美东)
中国学术界的不良之风还有一种比较诚实的没有被大家关注过，可是很行之有效。那就
是合作。
这里就是一个学术造诣颇深的院士和一个混迹学术界想要出头的高干千金的两厢情愿的
合作。
我们先来看一下一个手里控制着上亿元科研经费的中国最年轻的973首席科学家，973胚
胎发育干细胞项目首席科学家的基本情况：
谢欣研究员，现任中国财政部长谢旭人之女
现任 中国科学院上海药物所 研究员、 国家新药筛选中心模型建立 II 部主管、 课题
组长 。
下面是她所有的有名字的文章，还算诚实，没用同名同姓的凑数。
可是大家已经要问这样的条件够中科院研究员、百人计划择优资助吗？那些录用的评委
和百人计划的专家组这么一反以严格著称的常态了呢？
1.Chen D, Liao J, Li N, Zhou C, Liu Q, Wang G, Zhang R, Zhang S, Lin
L,... 阅读全帖 |
|
c**********c
发帖数: 81 | 33 来美国好多年了，一作发了三篇paper, 最早的10年发的，到现在也没攒几个citation
，只有靠review来加分了。发了很多邮件给editor，都没有回复的。跪求前辈们能给我
一些审稿机会，十分感谢。（之前就review过两次，都是别人给的机会）
研究方向：neurodegenerative disease
1. HIV 引起的神经性疾病 病理研究（Molecular mechanism of HIV-associated
neurodegeneratiove disease)
2. 神经干细胞发育和分化 （neuron stem cell development and differentiation）
3. 信号转导在神经分化中的作用，例如：Notch, Stat3, Wnt, mTOR, MAPK 等等（
Signaling transduction and its role in neuron stem cell development and
differentication)
4. 大脑衰老 （brain aging）
5. 星型细胞瘤和星型细胞... 阅读全帖 |
|
t******k
发帖数: 553 | 34 我不发表对二人是否结婚事情的任何意见，也不想知道，这个世界为博出位出名，手段多了去了。
裴院士，以前听过报告，有所认识。对于谢欣，这位最年轻的973首席科学家，海龟博士。开红色Audi TT的973兽吸，我还是第一次听说，无聊间，google了一下。
以下是转帖
引用:
这里就是一个学术造诣颇深的院士和一个混迹学术界想要出头的高干千金的两厢情愿的合作。
我们先来看一下一个手里控制着上亿元科研经费的中国最年轻的973首席科学家，973胚
胎发育干细胞项目首席科学家的基本情况：
谢欣研究员， 现任中国财政部长谢旭人之女
现任 中国科学院上海药物所 研究员、 国家新药筛选中心模型建立 II 部主管、 课题
组长 。
下面是她所有的有名字的文章，还算诚实，没用同名同姓的凑数。
可是大家已经要问这样的条件，没有一篇CNS系列的文章，1st-authored paper只有3篇
，够中科院研究员、百人计划择优资助吗？
那些录用的评委和百人计划的专家组，都糊涂了吗？这么一反以“严格”著称之常态了
呢？
1.Chen D, Liao J, Li N, Zhou C, Liu Q, Wang G, Zh... 阅读全帖 |
|
T******e
发帖数: 18290 | 35 一半脑细胞来自人类的小鼠，比同类更聪明
环球科技观光团 发表于 2014-12-04 08:00
（NoahTsang/译）有一半大脑细胞都来自人类的小鼠已经被创造出来了，这些小家伙比
它们的兄弟姐妹都要聪明。对此，精灵鼠小弟（Stuart Little）会怎么看呢？
这个想法可不是在模仿小说里的情节，通过对小鼠的整个脑部而非培养皿中的细胞进行
研究，我们得以更好地了解人类的脑部疾病。
被改造过的小鼠仍然拥有小鼠的神经元——这些负责“思考”的细胞占据了它们脑细胞
中半数左右。但它们脑中用于支撑神经元的神经胶质细胞几乎全都属于人类细胞。
“这本质上还是一个鼠脑，而非人脑。”纽约罗彻斯特大学医学中心（University of
Rochester Medical Center）的史蒂夫·古德曼（Steve Goldman）解释说，“但脑中
所有非神经元的细胞都属于人类。”
喧宾夺主
古德曼的研究团队从捐赠的人类胎儿当中提取了未分化的神经胶质细胞。他们把这些细
胞注射到小鼠幼仔体内，在那里它们将长成星形细胞（astrocytes），一种星形的神经
胶质细胞。
此后一年之内，鼠类的神经胶质细... 阅读全帖 |
|
a*****t
发帖数: 81 | 36
200 mv ? is it possible ?
Normally, rest membrane potential of neurons is about minus 50 to 80 mv,
as the outside membrane be the reference potential. For some astrocytes,
the value is about minus 20 to 30 mv. I can not recall the value for muscle
cells, but it is also minus.
You are right that you can assume the potential is distributed on the
membrane(double layer). Because compared with the resistance and capacitance
of the membrane, intra- and extracellular contributions c |
|
a******g
发帖数: 129 | 37 GFAP is actually not a reliable marker for these star-shape cells in vivo. |
|
a******g
发帖数: 129 | 38 it's an underestimated field.However, currently it lacks good in vivo and in
vitro system which prevent further clear characterization of these mystery
cells. |
|
p*****m
发帖数: 7030 | 39 阿 这个是另外一个问题 搞清楚glia什么功能当然有趣。我是说从发育角度 搞gli
al development还有什么特别有意思的问题没有
such
and |
|
a******g
发帖数: 129 | 40 actually the developmental biology of glial cells is very elusive.
No one exactly knows how they differentiate and where they develop from.
Maybe you can be the one:)) |
|
f***4
发帖数: 886 | 41 发育/起源应该与后来的功能相对应。oligodendrocyte相对好些，但是在成年时候的
oligodendrocyte祖细胞类的细胞，肯定不是行使表面上的功能。究竟做什么，还没搞
清楚；这个与发育过程也应该是相关的。 |
|
s*******g
发帖数: 3332 | 42 I thought his main interest was brain tumor initiating cell now.
talk
, |
|
a****d
发帖数: 1919 | 43 The current opinion is that NSCs give birth to neurons and glia cells. The
neural to glia switch during cortical development has been studied in recent
years. Check Freda Miller's work and others for more details. |
|
f***4
发帖数: 886 | 44 Freda Miller 不算这个领域的吧。glia中包括的很多，发育模式道理上应该是不一样
的。
看来，这个方向确实很多东西未知阿
recent |
|
d******r
发帖数: 124 | 45 最近在培养neuron，今天是第四天，发现很多细胞聚集在一起，中间的看起来分化得也
不好，neurite很短，如图所示。请高人鉴定一下，这些是不是neuron？可能是什么原
因造成的？我是从E18 mouse embryo分离的neuron，用波片倒扣在astrocyte
monolayer上培养。medium用的是neurobasal+B27。谢谢！ |
|
d********a
发帖数: 1947 | 46 大家觉得mitomycin c 还是 arabinose c better for stopping cell growth in
astrocytes? (for a migration assay) |
|
d******r
发帖数: 124 | 47 没有的
波片和astrocyte layer之间有空隙，这样神经元并不和胶质细胞有直接接触。 |
|
F******p
发帖数: 2099 | 48 波片和astrocyte layer之间有medium, virus加到medium里面，virus自然会find its
way. |
|
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