a******u
发帖数: 211 | 1 Expert Rev Vaccines. 2007 Oct;6(5):785-96.
Cationic liposomes as vaccine adjuvants.
Christensen D, Korsholm KS, Rosenkrands I, Lindenstrøm T, Andersen P,
Agger EM.
please send the paper to e*********[email protected]
Thanks so much! |
|
|
n***3
发帖数: 663 | 3 J Control Release. 2008 Aug 25;130(1):22-8. Epub 2008 May 15.
Reactive oxygen species play a central role in the activity of cationic
liposome based cancer vaccine.
Yan W, Chen W, Huang L.
Please send the paper to e*********[email protected]
Thanks a lot!!!!! |
|
n***3
发帖数: 663 | 4 1.J Control Release. 1999 Aug 27;61(1-2):233-40.
Positively charged liposome functions as an efficient immunoadjuvant in
inducing cell-mediated immune response to soluble proteins.
Nakanishi T, Kunisawa J, Hayashi A, Tsutsumi Y, Kubo K, Nakagawa S,
Nakanishi M, Tanaka K, Mayumi T.
2.J Control Release. 2008 Aug 25;130(1):22-8. Epub 2008 May 15.
Reactive oxygen species play a central role in the activity of cationic
liposome based cancer vaccine.
Yan W, Chen W, Huang L.
Please send the paper to em... 阅读全帖 |
|
n***3
发帖数: 663 | 5 Int J Pharm. 2009 Feb 23;368(1-2):56-62. Epub 2008 Oct 15.
The effects of salt on the physicochemical properties and immunogenicity of
protein based vaccine formulated in cationic liposome.
Yan W, Huang L.
Please send the paper to e*********[email protected] |
|
n***3
发帖数: 663 | 6 1. J Physiol. 2010 Apr 15;588(Pt 8):1227-40. Epub 2010 Mar 1.
The calcium-permeable non-selective cation channel TRPM2 is modulated by
cellular acidification.
Starkus JG, Fleig A, Penner R.
2. Prog Biophys Mol Biol. 2010 Sep;103(1):18-27. Epub 2010 May 27.
Chemical physiology of oxidative stress-activated TRPM2 and TRPC5 channels.
Yamamoto S, Takahashi N, Mori Y.
Please send the papers to e*********[email protected]
Thanks! |
|
C*******e
发帖数: 4348 | 7 谢谢!用的就是这个guide
选出来三个
Capto S
SP
CM
然后Capto S比后两个贵一倍
不想用FPLC是本来就是粗提
条件摸好了以后gravity column也比较快
然后后面再上亲和层析(有tag)
还有一个是我们没有空柱子
然后这个县Cation exchange再Affinity的想法我没有试过
不知道好不好使
所以不想一下子再买好多东西
就想gravity column先试试
http://www.gelifesciences.com/aptrix/upp00919.nsf/Content/86545
257628001CC7BA/$file/18112731AH.pdf |
|
C*******e
发帖数: 4348 | 8 谢谢！
不过这里我还是不太想用PBS buffer
因为要跟在cation exchange chromatography后面做
所以最理想的是用HEPES之类 |
|
D*a
发帖数: 6830 | 9 http://www.nature.com/news/2011/110427/full/472391a.html?utm_so
Peer review of scientific papers in top journals is bogged down by
unnecessary demands for extra lab work, argues Hidde Ploegh.
Hidde Ploegh
Submit a biomedical-research paper to Nature or other high- profile journals
, and a common recommendation often comes back from referees: perform
additional experiments. Although such extra work can provide important
support for the results being presented, all too frequently it represents
ins... 阅读全帖 |
|
p*****m
发帖数: 7030 | 10 neuroscience板上谁贴了Ed Boyden写的关于channelrhodopsin的回忆 看着很有趣。
他提到一点，当时他们那片paper一开始投了science悲剧，理由是“we hadn’t made
any scientific discoveries beyond finding that channelrhodopsin-2 functioned
well in neurons”，这话单独来看道理上无比正确。在这片Paper之前，大家已经知道
Chr2能感光也能响应光开放cation channel了，所以这篇文章确实只说明Chr2能在神经
元里表达并工作而已，什么新机制都没提出。后来发了Nat. Neurosci.
但是呢？把Chr2和neuron两个概念联系在一起，不需要任何别的机制，就已经是革命性
的突破了，因为可以从此用光很有效的（这个定语也重要）控制神经元的活动了。这个
东东弄个Nobel不成问题，如果Chr2自己发展的好，Ed和Karl分享这个nobel也不是完全
没有可能，尽管不大。可惜reviewers看不到，人家就觉得没有机制，没有新发现，完全
不... 阅读全帖 |
|
v******l
发帖数: 235 | 11 求Paper, 请发到 j********[email protected]. 包子答谢!
PMID: 19546862
[PubMed - indexed for MEDLINE]
Nat Rev Nephrol. 2009 Aug;5(8):441-9. Epub 2009 Jun 23.
The role of transient receptor potential channels in kidney disease.
Woudenberg-Vrenken TE, Bindels RJ, Hoenderop JG.
Source
Department of Physiology, Radboud University Nijmegen Medical Centre,
Nijmegen, The Netherlands.
Abstract
The transient receptor potential (TRP) superfamily consists, in mammals, of
six protein subfamilies, TRPC, TRPM, TRPV, T... 阅读全帖 |
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l**********1
发帖数: 5204 | 12 >Inorganic Additives
There are several different types of inorganic additives in blood collection
tubes. These include those that are completely inorganic in composition, (
eg, silica and sodium fluoride), and those that are alkaline metal salts
of organic acids, [(eg, disodium ethylenediaminetetraacetic acid (Na2EDTA),
K3 or K2EDTA, trisodium citrate, and potassium oxalate].
Most dry additives tend not to be a limiting factor in determining the shelf
life of the evacuated tube.
For hematology a... 阅读全帖 |
|
s*******7
发帖数: 160 | 13 下周有一个重要面试，是一个研究神经退行性疾病和衰老的机构，他们要我设计一下以
后自己可能开展的研究课题或工作。我实在是不知道该怎末下手，我的背景是以前做过
神经元甘氨酸受体通道的电生理研究，后来做过GABAA受体还有protocadherin蛋白的研
究，但我对老年痴呆之类的一点概念都没有，现在上网查也只能了解各大概，也没找到
老年痴呆和我熟悉的GABAA受体或是protocadherin之间有关系的相关文献，有没有高人
指点一下该怎末入手设计这个面试课题啊，万分感谢。
补充一下，先前通email他们好像对我的电生理研究背景比较感兴趣，另外我熟悉的实
验技术包括细胞培养，免疫荧光，western blot，基因克隆等。还有我查了一下，他们
研究所有人做β-Site APP Cleaving Protein 1 (BACE1)和CutA Divalent Cation
Tolerance Homolog (E. coli) (CUTA)的研究，还有人做AD小鼠模型。
现在急死了，多谢大家指点啊，要有好的idea我虽然不富， 但诚心愿意送上包子感谢
。 |
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z*******a
发帖数: 175 | 14 pictures from confocal microscopy on a small sized tissue
have to do quantification using imageJ
got ~2 fold difference between 2 groups
questions:
are intensities of immunostaining are stoichiometric?
variation is so big; seems not believable and not reproducible even by
myself; so is the quantification of immunofluorescence
meaningful at all? especially in tissue instead of cultured cells |
|
b******s
发帖数: 5365 | 15 超过10个transmembrane domains? 我PhD做的就是个cationic amino acid
transporter, 14 transmembrane domains, 但和肿瘤没关系。 |
|
b******y
发帖数: 627 | 16 For LZ,
sunnyday's desalting method is the best to try. I will be surprised if it
doesn't work. I guess you have to use a syringe because nobody likes to
inject 32P into their FPLC. In that case, you should be careful of the
fraction size. Don't mix two well separated peaks during fractionation.
kaka1000's vivapure spin column离子交换柱 is a cation/anion exchange column,
to which unlimited volume can be loaded. In the end, elute with B buffer.
The peak is sharp because the salt concentration is high ... 阅读全帖 |
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s********n
发帖数: 2939 | 17 离子交换是根据pI来的，pH>pI，anion exchange; pH
用size exclusion会比较方便 |
|
s********n
发帖数: 2939 | 18 离子交换是根据pI来的，pH>pI，anion exchange; pH
用size exclusion会比较方便 |
|
w********m
发帖数: 1137 | 19 Lu, J., Langer, R. and Chen, J., A novel mechanism is involved in cationic
lipid-mediated functional siRNA delivery, Molecular Pharmaceutics, 6: 763-
771, 2009.
删掉那些文章，大概简历上的都是真的 |
|
c****r
发帖数: 576 | 20 这个是那个真正在多伦多大学拿博士学位的台湾人，James J.Lu，看文章原始链接：
http://pubs.acs.org/doi/abs/10.1021/mp900023v
A Novel Mechanism Is Involved in Cationic Lipid-Mediated Functional siRNA
Delivery
James J. Lu†‡, Robert Langer†§ and Jianzhu Chen*†&#
8225;
Koch Institute for Integrative Cancer Research and Departments of Biology,
Chemical Engineering, and Biological Engineering, Massachusetts Institute of
Technology, Cambridge, Massachusetts 02139
Mol. Pharmaceutics, 2009, 6 (3), pp 763–771
DOI: 10... 阅读全帖 |
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t*******3
发帖数: 2005 | 21 我不认为那个多伦多的博士学位是假的,理由如下：
1。有id在生物版里说陆骏申请千人计划的时候提供了MIT的Robert Langer的推荐信:
发信人: zyh (CH2O), 信区: Biology
标 题: Re: 一件奇怪的事
发信站: BBS 未名空间站 (Fri Jul 27 07:45:16 2012, 美东)
陆骏 claimed that he was a postdoc from Robert Langer and was working at
Merck before his application. He applied for 1000 young talents through two
universities at the same time. In his application package, he provided the
recommendation letter from Langer.
==============================================
2。有人查到Langer和lu Jun（非耶鲁卢军）合作的文章：... 阅读全帖 |
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q*******8
发帖数: 768 | 22 迄今发现英语里最TM搞的是阴阳离子和阴阳电极，anion么是阴离子，到anode了就变阳
极了；反之cation是阳离子，到cathode就变阴极了。。。反正我到现在都搞不清，不
知道欧洲人一边用加减号一边说着正反英语是怎么分清楚的。。。 |
|
d******n
发帖数: 29 | 23 1. Multiproduct High-Resolution Monoclonal Antibody Charge Variant
Separations by pH Gradient Ion-Exchange Chromatography
Anal. Chem., 2009, 81 (21), pp 8846–8857
2.Protein separations with induced pH gradients using cation-exchange
chromatographic columns containing weak acid groups.
J Chromatogr A. 2008 Feb 15;1181(1-2):83-94. Epub 2007 Dec 26.
My Email: d******[email protected]
Thank you so much for your help!!!! |
|
E******g
发帖数: 170 | 24 Cao E, Liao M, Cheng Y, Julius D (2013) TRPV1 structures in distinct
conformations reveal activation mechanisms. Nature 504:113-118.
Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D (1997)
The capsaicin receptor: a heat-activated ion channel in the pain pathway.
Nature 389:816-24.
Caterina MJ, Julius D (2001) The vanilloid receptor: a molecular gateway to
the pain pathway. Annu Rev Neurosci 24:487-517
Cosens DJ, Manning A (1969) Abnormal electroretinogram from a Drosophila
m... 阅读全帖 |
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k*****1
发帖数: 454 | 25 我列一下几篇开创性的文章吧
Hegemann是毫无疑问可以拿诺奖的，
Braun, F.-J. und Hegemann, P (1999). Two light activated conductances in the
eye of the green alga Volvox carteri. Biophys. J, 76,1668-1678.
Nagel, G., Ollig, D., Fuhrmann, M., Kateriya, S., Musti, A.-M., Bamberg, E.,
Hegemann, P. (2002) Channelrhodopsin-1, a light-gated proton channel in
green algae. Science 296, 2395 - 2398
他的薄厚Nagel除了在上面那篇Science的开创性文章里面做出了了ChR1之外，又在03年
的另外一篇PNAS文章里面做出了ChR2。这里的突破就是在动物细胞里面做出了ChR2，而
ChR1只是在藻类细胞里面。这是第二个飞跃。不知道ChR2的功劳是算到Nagel还是
B... 阅读全帖 |
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n***3
发帖数: 663 | 26 Nanomedicine. 2013 Dec 19. pii: S1549-9634(13)00714-4. doi: 10.1016/j.nano.
2013.12.003. [Epub ahead of print]
Cationic lipid nanocarriers activate Toll-like receptor 2 and NLRP3
Inflammasome pathways.
Please send the paper to e*********[email protected]
Thanks! |
|
m*********D
发帖数: 1727 | 27 (ZT)
Salt concentration
The phosphate groups are moderately strong acids, and thus they are always
ionized at physiological pHs. The two oxygens are equivalent, so they share
the single negative charge. These negative charges tend to blow the double
helix structure apart, which is perhaps why I haven't included them in the
forces that hold the helix together. Why is the helix stable?
Inside the cell, the monovalent salt concentration is about 0.15 M. The
major cation in the cell is K, while i... 阅读全帖 |
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C*********h
发帖数: 83 | 28 大致读了文章的摘要，应该是cell death领域的同行，做的方向主要还是apoptosis的
下游，呼吸链如何影响apoptosis的，以及cancer specific apoptosis以及ER-
mitochondria contact。
从Grimm的文章来看，他即便不是杰出的科学家，也是一个decent scientist。希望他
Rest in Peace.
如这篇review，citation 139.
The permeability transition pore in cell death.
Apoptosis. 2007 May;12(5):841-55. Review
这篇2011的EMBO J的citation是 81
Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform
for apoptosis induction
Apoptosis重要的东西基本都做的差不多了，确实没什么可以做的。大lab都在做
physiology,做各种小鼠模型。Grimm凭一己之力做... 阅读全帖 |
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发帖数: 1 | 29 Try Ca for DNA experiments.
Try the same experiments shown on S figure 2 by using DNA instead of RNA as
a substrate.
Han Lab's Mg reagent may be contaminated with Ca or other metal cations.
Good luck to all |
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z**c
发帖数: 7595 | 30 老革命遇到新问题。
要把固态物分散成以水为分散介质的Emulsion或Suspension。要求粒径在2-20 microns
之间，anionic或cationic particles，常温稳定，Shelf Life一天足够，当然越长越
好。 浓度没要求，别太稀太稠，能够泵输。
基质和水的密度接近，不溶于水，惰性。 我觉得做成稳定的Suspension应该比较困难
，正好它溶于丙二醇及芳香溶剂，把它先液化然后再做成Emulsion比较好。
做Painting和Coating的当然是专家了，但都是核心机密，不会告诉我的，虽然我根本
不涉及他们的领域。 网上也少有资料。
我搞出一个配方，但觉得应该有足够的空间可以提高，哪位高手能有建议没有？
包括：乳化剂及用量，乳化器及乳化条件，等等。先谢过。 |
|
e****n
发帖数: 165 | 31 有人用过TSK silica gel column 分离过cationic polymer么？Is low pH and high
salt going to work? 向高手请教，谢谢！！ |
|
M****Q
发帖数: 26 | 32 ACN 比例加大？
flow rate 加大？
C8, C4, 或者cation exchange, anion exchange column试试？ |
|
c****r
发帖数: 36 | 33 偶很土，从开始英语就很烂，发音不行
最简单的。。。问题
cation,阳离子这个， 用的金山词霸念“开审”. Lingoes念"开太恩"
这个还好说，偶一般念后边那个。
但是crystallization呢，念"crystal-来贼审" 还是 "crystal-太嗯"？
基本上，遇到lization等偶都习惯性的拼成"来贼审"...
但是在词霸和lingoes上的发音大多是"太嗯".
身边没人指导，求助大家怎么念比较合理，地道
多谢了 |
|
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d*******g
发帖数: 3972 | 35 还好吧，tion结尾的发音都差不多，似乎也就cation这种比较特殊？ |
|
a******z
发帖数: 1464 | 36 cation念开太恩
crystallization念crystal乐贼深吧：） |
|
x****g
发帖数: 72 | 37 Cation = cat + ion
anion = an + ion
then you can pronounce it... |
|
m*****e
发帖数: 1506 | 38 Karen Wooley组最近有工作，去查关于cationic shell-crosslinked nanoparticle的
文章吧。要从PAA-PS转换过去。 |
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c********r
发帖数: 2227 | 39 Cationic quaternary polyelectrolytes. Literature review.
Hoover, Merwin F. Calgon Corp., Pittsburgh, PA, USA.
Journal of Macromolecular Science, Chemistry (1970), 4(6), 1327-417.
比较老的文献
麻烦email到m************[email protected]
或者任何其他方式。
包子答谢～ |
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n*****g
发帖数: 4117 | 40 ☆─────────────────────────────────────☆
ylide (叶立德) 于 (Sat May 2 23:59:34 2009) 提到:
复习一下高中内容，温故而知新
把一个电学元件（化学电源、蓄电池、电解电容、二极管等等）看作一个整体，则电流
（正电荷）流入的一端称为阳极anode，电子（负电荷）流入的一端称为阴极cathode，
这是普遍适用的原始定义。
衍生词汇有：阳极化anodize，阴极射线cathode ray
与此有关的概念是正极“+”和负极“-”。正极指电势较高的一端，负极指电势较低的
一端。在大多数情况下，阳极和正极、阴极和负极是重合的，因为电子会自发从电势较
高的一端流出。但是对于化学电源和原电池而言是个例外，因为化学势的存在使得电子
能够克服电势差反向流动，使化学能转化为电势能，所以此时阳极是负极，阴极是正极。
另外容易混淆的是阴离子anion和阳离子cation。这两个术语原来分别指在电解质溶液
中趋向anode和cathode的离子，曾被翻译为“阳向离子”和“阴向离子”，现在泛指带
负电荷和正电荷的离子。
☆───── |
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s********n
发帖数: 235 | 42 Title: CATION EFFECTS ON P-31 MAS NMR CHEMICAL-SHIFTS OF METAPHOSPHATE
GLASSES
Author(s): BROW, RK; PHIFER, CC; TURNER, GL, et al.
Source: JOURNAL OF THE AMERICAN CERAMIC SOCIETY Volume: 74 Issue: 6
Pages: 1287-1290 Published: JUN 1991
Thanks a lot!
My email: x********[email protected] |
|
s********n
发帖数: 235 | 43 Cation Effects on 31P MAS NMR Chemical Shifts of Metaphosphate Glasses
Journal of the American Ceramic Society
Volume 74, Issue 6, Date: June 1991, Pages: 1287-1290
Thanks a lot! |
|
h******g
发帖数: 600 | 44 【作者】:
Coe, Benjamin J.; Curati, Naomi R. M.; Fitzgerald, Emma C.
【文题】:
Unusually facile syntheses of diquat (6,7-dihydrodipyrido[1,2-a:2\',1\'-c]
pyrazinediium) and related cations.
【期刊名，年份，卷，起止页码】:
Synthesis (2006), (1), 146-150.
【链接】:
【求助者email】:
f**********[email protected]
【求助者是否发包子】: |
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s**q
发帖数: 177 | 45 HPLC 高手帮忙看看SCX column和我们使用的方法。
有人用过这个柱子吗？为什么，我们的每次总是用了几次后，用同样的标准样（std）
去测，peak area 值就会变大呢（相对标准偏差还是很小，也就是系统的rsd还是好的
，就是同样的东西，测出的peak area 变大了）？看起来这是一个cation-exchange
column，有什么要注意的呢？有什么方法能恢复呢？
另外，通常HPLC的柱子使用寿命是多久呢？我们的这个新柱子刚用了一个月，就变成这
样了。我们对于UNKNOW的值得测定是建立在unknown和std的peak area 的比较上建立的
，而不是建立标准曲线。所以直接导致unknown里的含量结果偏低。所以，我也有点怀
疑这个方法的有效性。
当然，我们每天要测20个样品左右。。这个会用影响吗？我都不好意思跟老板讲这个柱
子又有问题了：（
谢谢 |
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c******x
发帖数: 438 | 48 I am looking for a paper, see the information below
http://www.emeraldinsight.com/journals.htm?issn=0369-9420&volume=37&issue=1
Synthesis and characterisation of 9,9-bis(4-hydroxyphenyl)-fluorene
catalysed by cation exchanger
W. Liu, J. Wang, Q.H. Qiu, L. Ji, C.Y. Wang, M.L. Zhang (pp. 9 - 15)
Pigment & Resin Technology vol. 37, issue 1, 2008
Thanks in advance. |
|
y*******4
发帖数: 72 | 49 这是一个类似cryptand的一个柱子，只是cation exchanger是其他化合物，流动相是
CH3SO3H，浓度是10mM,流速0.5ml/min,柱子长35mm,用ICS3000时 三个离子的保留时间
都在1.3min左右，用DX600，Na 和K保留时间0.8min，Ca大概2min，用CS12A试时，相同
条件下，钠的保留时间大概8分钟左右，钙大概28分钟，ICS3000因为别人也在用，
injection loop是2ml,这可不可能是原因呢 |
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