p**********u
发帖数: 15479 | 1 昨天一房间白人培训，中间就我一个是老中。然后是quick thinking.好比有人问你，
pussy.你要1秒内加上一个形容词。于是白人呢，要么是wet,tight,pink,轮到我，变成
了hairy pussy.
结果被人笑话了。说是典型的culture difference.她们白人都刮阴毛。
女的不刮阴毛。男的不割包皮。老中就是脏，sub human。 |
|
|
O**n
发帖数: 649 | 3 在上班路上发现15 East 40th Street的垃圾箱里堆满的十几箱的崭新的中文教材。 手
贱就看了一下，发现这些教材都是国务院侨务办公室无偿赠送给Asian Cultural
Council (ACC)用来给美国的中文学校的。教材印刷的非常精美，彩色插图，还有单独
的练习册，简体，繁体的版本都有，就被这帮人一下全部丢到垃圾堆了。国内的小孩有
的连书都买不起，这边的烂人，唉！ |
|
f******p
发帖数: 34 | 4 There will be many fun and entertaining activities including dragon & lion
dancing, Shaolin kung fu demonstrations, Chinese cultural dancing, opera,
puppet show, and many Chinese instrumental music presentations. Food vendors
will also be on hand for hungry folks. See event link for complete schedule
along with a $2 discount coupon.
http://www.portlandchinesetimes.us/cny08/eindex.htm |
|
h****e
发帖数: 296 | 5 我有8張免費TICKETS FOR CHINESE NEW YEAR CULTURAL FAIR
IT'S ON FEB-05-2011 10:00AM-6:00PM
OREGON CONVENTION CENTER HALL C
777 NE MARTIN LUTHER KING JR BLVD
PORTLAND OR 97232.
I BEEN THERE LAST YEAR. IT'S OK. KIDS UNDER 6 YEAR OLD ITS FREE.
THERE LOTS FOOD AND FREE STUFF TO GET. |
|
b*******y
发帖数: 98 | 6 想在92130那块开个chinese language and culture 的周末班，大概1小时左右，免费
参加
就是每周给小朋友讲个故事（目前考虑每周讲一回西游记，或者讲讲山海经之类的故事
。。。），学个唐诗 （诗经也可以，看小朋友年纪），还有就是念《三字经》
大家知道有什么地方可以办么？pacific highlands ranch的recreation center 里有
房间可以使用么。。。
还是一定得去和周边图书馆商量 |
|
C*****C
发帖数: 66 | 7 Learn Chinese & practical conversation with Native Chinese speakers. Travel
to China to experience real China.
Our Chinese language and culture program is free to public, will start on
fall semester, space are limited !!!
Our China trip will begin in Shanghai on September!!!
Please come & sign up for your spot, test your level to join appropriate
class at the Orientation. (You are welcome to bring friends. No Chinese
language skill is required.)
When: Tuesday, May 17(Get a chance to win prize)
T |
|
m**i
发帖数: 394 | 8 This social network is across culture and mission is to help the community.
Everyone can share experiences there sincerely.
http://tigerparents.ning.com/ |
|
b****y
发帖数: 480 | 9 上礼拜烙印大老板开会，手下的managers 基本都是烙印，要求招人一定要
culture fit。manager 门个个心领神会。其中一个只招烙印，interview 时有一老美
极力反对招这个阿三，因为啥也不懂 ，阿三经理根本不当回事，给了offer，非常好的
package 和 level 都很高。我知道有几老中的 candidates，烙印连电话都
不打就reject 了。这就是目前和未来的硅谷老中就业和未来 |
|
g********s
发帖数: 3652 | 10 什么culture fit？ 印度文化吧？要求说印度话吗？
中国人和印度人区别是，中国人太静太蔫。印度人凶猛 |
|
o**********e
发帖数: 18403 | 11 感谢LZ。 别逼LZ。 他知道贴大字报。
不过大字报确实可以指名道姓的。
很多老中默默到被炒，连大字报
都不敢贴呢。 这是进步！
心动不如行动，否则很快就没有
机会了。 加入行动的ITRelief
G group吧：
https://groups.google.com/forum/#!forum/itrelief
加入灌水的ITRelief的俱乐部吧。
http://www.mitbbs.com/club_bbsdoc/ITRelief.html
不要觉得烙印好可怕啊。。。 啊啊啊！
teaparty, occupy wall street可以爆发。
西雅图anti-WTO protest 也可以爆发。
老美老中都不可小觑。
他越猖狂，得罪的人越多，漏洞越多。
墙倒众人推， 我们退无可退，
只有奋起。
即使是偷偷奋起写大字报，
也是奋起。 逆来顺受
只有向老华工一样被
赶出去的结局。
你留点证据什么“culture fit"，
和其他歧视性说法吧。 他们一向
是撒谎不眨眼的，栽赃陷害
也干的出来。 |
|
J*******g
发帖数: 381 | 12 【 以下文字转载自 Apple 讨论区 】
发信人: JiayiWang (noname), 信区: Apple
标 题: Apple's Corporate Culture: 10 Lessons for Staying in Steve Jobs' Good Graces
发信站: BBS 未名空间站 (Tue Aug 10 23:56:17 2010, 美东)
When it was announced that Apple senior vice president Mark Papermaster left
Apple, it sent shockwaves
through the tech industry. Papermaster came to Apple from IBM after the
companies waged a short-lived
battle over exactly when he could start working at the hardware company.
After that, he took over the iPhone |
|
x**e
发帖数: 1794 | 13 "A Glimpse of China - Seattle Chinese Art and Culture Festival 2011" at Seattle Center this Saturday, 05-21-2011.
http://chinaartandculture.org/
好像还没人贴这个吧 |
|
x*****4
发帖数: 204 | 14 看到有Polynesian Cultural Center的coupon是buy one admission ticket get one
free up to 45.00。
我们有两个人去的话应该买几张呢？
多谢啦。
xiayu |
|
l******d
发帖数: 2186 | 15 我们打算去那个Culture Center看那个表演，但是不想吃那里的Buffet，可是我看到哪
个Day Experience 和表演之间差了2个小时，有没有和我们一样没有吃Buffet的同学？
你们都是怎么打发时间的？不吃Buffet，附近有没有什么别的地方可以吃饭？有什么推
荐么？
谢谢啦~~~ |
|
r*****g
发帖数: 123 | 16 转载一篇 Charles Rosen 发在十一月份的 New York Review of Books 上的文章。题目
很大， Rosen 和以前一样，除了几声叹气以外，并没能提供一个稍微系统一点的描述或
解释。他的精英主义怀旧情绪也让我觉得很别扭。不过他的文章总有很多轶事，读起来挺
消遣。这篇文章里讲的 Gould 和 Stuttgart 的 Siegfried 就很有意思。
Culture on the Market
By Charles Rosen
1.
Some thirty years ago, the then head of CBS Records, Clive Davis, sent out a
directive that no recording was to be undertaken if the recovery of its costs
could not be projected within one year's sale in the United States.
Accordingly the plan to record Mozart's Sere |
|
k****z
发帖数: 550 | 17 我是南方人，小时候几乎从来没吃过饺子。过年年夜饭十分丰盛，就更不可能吃饺子了
。大概也是大学时候到北京过一次年才看到大家都吃饺子，都看春节晚会。确实是
culture shock。 |
|
M********t
发帖数: 5032 | 18 人均2万元只吃汉堡 难怪
特朗普要打脸安倍(组图)
头条ABC
大实话
12小时32分钟前
近年日本的观光立国政策是搞得有声有色，优质的服务，实惠的价格，吸引了越来越多
的人来日本观光旅游，但大部分游客来日本就知道扎堆在电器店里买马桶盖，说起吃就
只能想到寿司或者拉面。
深度游的推广做得极其不到位，这可急坏了安倍。于是他一拍脑门，邀请一个流量
过亿的网红做个深度示范游吧，这个人就是川普。
于是就有了川普夫妇从11月5日到7日为期三天的正（shen）式（du）访（guan）问
（guang），名为加强日美同盟，实际上你懂的。
但安倍心有余而力不足，国库财政紧张，不能给川普最高礼遇——“国宾”，而是
作为“公式实务访问宾客”邀请来的。这其中有什么区别呢，请看下表。
另外，日本方会负担受邀方10人以内的出行费用，这一点不论是国宾还是访问宾客
都是一样的。这样看来其实两种都差不多呢，在日本，同一家餐厅午餐会比晚餐便宜，
想必皇宫内也是这样在算账的呢，真是勤俭持家。
当然这是开玩笑，真实理由大概是因为川普黑料太多了，日本国内很多人讨厌他。
在这几日的行程中就有不少民众反对游行，考虑到这一情况，安... 阅读全帖 |
|
|
|
b********n
发帖数: 38600 | 21 https://www.amazon.com/Hillbilly-Elegy-Memoir-Family-Culture/dp/0062300547
Christians are more likely to drink to excess, be unfaithfult to a spouse,
divorce and physically abuse a spouse or child than the non-religious or
less religious. Evangelical Christians are more likely than moderate
Christians to engage in those behaviors. Religiosity directly corresponds to
the above mentioned behaviors. But don't let facts get in the way of your
preaching. |
|
h*******e
发帖数: 1968 | 22 E. Perry Link, Professor of Chinese Language and Literature
of
Princeton University, gave a talk entitled "Ten Good Things
About
Chinese Culture" on this Friday, October 20.
He said:
1. food
2. Tang poems
3. calligraphy
4. rhythms
5. humor
6. wholism
7. morality performing
8. loyalty
9. Quan4 (advising, persuading)
10. accessibility |
|
i*****l
发帖数: 209 | 23 P.5
A year and a half had elapsed since the close of the Franco-German War. Never
had the waves of Greman selfesteem run so high. The exultation of victory had
passed into a tumultuous self-glorification. The universal view was that
German culture had vanquished French. Then this voice made itself heard,
saying ---
Admitting that this was really a conflict between two civilisations, there
would still be no reason for crowning the victorious one; we should first have
to know what the vanquished o |
|
h*******x
发帖数: 12808 | 24 Dear Alumni,Please find attached an upcoming program at China Institute on J
anuary 15th: Rethinking Memory, History and Culture from One Corner of Beiji
ng University. It will be an evening with Vera Schwarcz, author of "Place an
d Memory in Singing Crane Garden" (Ming He Yuan, Beida). Prof. Schwarcz is D
irector of the Freeman Center for East Asian Studies at Wesleyan University
and a well-known historian, poet and writer of numerous books. She visited B
eida as a member of the first group of |
|
d**********u
发帖数: 4124 | 25 ☆─────────────────────────────────────☆
handsomex (潇潇细雨) 于 (Tue Dec 23 23:39:28 2008) 提到:
Dear Alumni,Please find attached an upcoming program at China Institute on J
anuary 15th: Rethinking Memory, History and Culture from One Corner of Beiji
ng University. It will be an evening with Vera Schwarcz, author of "Place an
d Memory in Singing Crane Garden" (Ming He Yuan, Beida). Prof. Schwarcz is D
irector of the Freeman Center for East Asian Studies at Wesleyan University
and a well-known hist... 阅读全帖 |
|
h**f
发帖数: 165 | 26 Culture clash in China
An online row highlights the need for Chinese universities to fix their
hiring policies. In October, an online war broke out between Yi Rao, a
neuroscientist
and Peking University’s dean of life sciences, and Keming Cui, a plant
biologist and professor emeritus at the university who has a string of
positions on academic and awards committees and editorial posts on Chinese
journals to his name.
Cui retired from Peking University four years ago but kept his laboratory
there. |
|
c***i
发帖数: 5 | 27 【 以下文字转载自 ChinaNews 讨论区 】
【 原文由 Chili 所发表 】
esp. for daigaku
read about a post on Chinese words in the traditional form just in here,
and dont bother to find it, the point i try to make is self bolstered
back in 3 years time when i flewfrom az to wa, a japanese old man(70)
talked with me across the aisle we were sitting aside,
this man is as kind as any japanese, and tried to make the conversation
he praised chinese traditional culture and esp its language. i still
clearly remember his use of th |
|
t*x
发帖数: 1065 | 28 I do mid-brain cultures from mouse embryos. So far, my cells are not
consistent. Sometimes they spread out individually (great), but sometimes
they clump up as clusters. It looks like a coating problem. I coated with
poly-D-lysine at 0.1mg/ml overnight and washed with water twice and then
10ug/ml laminin coating overnight. After that, wash by water twice and dried.
Anyone has suggestion about a coating technique that is consistent with cell
spreading (not clumping)?
Thanks a lot! |
|
a****d
发帖数: 1919 | 29 for Ca2+ P method, make sure the pH~ 7.04; after adding the mixed plamids,
buffer, wait only half hour, then wash out the transfection medium as clean
as possible(you should be able to see only the black aggregates on the cell
surface, but not other places), then add fresh warm culture medium.
You can also try Fugene HD from Roche, which is better and less toxic
compared to lipofectamin and similar products.
you can also grow cortical neurons on top of glia cells to preserve activity
and help ce |
|
n********k
发帖数: 2818 | 30 Hi, folks:
Any of you do primary mouse neuron culture? I like to know which stage
one can easily derive primary
neurons, the later the better for me... We have protocols for embryonic
neurons but I'll like something later
than that but aslo need to be healthy and can tolerate with some
manipulation later on such as Cre-viral
infection.... |
|
h******u
发帖数: 602 | 31 Try to treat cells with a chemical. This chemical is easy to dissolve in
DMSO. However, if adding to culture meidum, it precipiates.
How to solve this problem? Lower the concentration?
Thanks~~~~~~~~ |
|
L****S
发帖数: 76 | 32 Dear all,
I need to culture some mammalian cells in Collagen I in hope of getting 3D,
and then I need to get the cells out of collagen I to get either mRNA or
protein.
I got some information from Sigma, but there is no way for us to know how to
use the collagenase to get rid of collagen I.
Is there anyone knows from which company I can get the right Collagen I and
Collagenase,please?
Thank you so much.
Lei |
|
h********n
发帖数: 4079 | 33 Can you buy collagenase (I am not sure about subtype) and use as trypsin.
a few years ago I used collagen coated dish to culture cells, when I need to
harvest cell, I used a mixture of collagenase and trypsin.
you might need to optimize the system. |
|
|
h***3
发帖数: 26 | 35 i know this sounds extremely stupid...
I am new in biology
I did the E coli (SURE2) transformation in the cell culture hood... as i
remember I did not drop the bacteria cells out, just some LB media...
I spray the hood with 70% ethanol for many times, and turn the UV light for
10min,
is it possible for the bacteria cells to contaminate the laminar flow hood???
What should I do to de-contaminate the hood?
help me! I do not want to get this trouble...
Thanks a lot!! |
|
m*****n
发帖数: 760 | 36 1. do not wash the plate.
2. add 4% PFA into the well immediately after you withdraw the medium
(one well each time, never let the well dry)
3. or add same volume of 8% PFA directly into the culture
without the withdrawl of the medium.
4. 10 minutes fixation, then wash with PBS. |
|
|
m**********d
发帖数: 137 | 38 我们实验室也发生过类似的情况，最后证明是yeast contamination，用以下的
protocol很快就解决问题了
13ml complete culture media + 100-500 microlitter amphotericinB(depend on
your cell line) + 1ml pen/strep antibiotic
treat之后你在显微镜下能看到黑点死了漂起来，大概treat3-4d, trypsinize,
replate之后就很干净了，不放心的话再做一下hoechst staining。 |
|
m******g
发帖数: 69 | 39 我们买了一种小的LNA,用了沉默一种miRNA,可是用什么样的条件才能transfect LNA到
cultured cell里面，来达到沉默miRNA. 谁做个相关的实验，有paper和protocol吗，
我是本科生，老板让我做这个，没什么头绪。 |
|
s*****3
发帖数: 41 | 40 最近打算用free-style 293F 细胞来表达一些蛋白。 293F cells 是invitrogen公司
从293 细胞adapted的悬浮细胞。 我从invitrogen公司买了这个细胞和其相应的培养基
后， 在普通的incubator （没有shaker）里面培养，第一次复苏完全没有成功。后来
又向 invitrogen要了一管， 复苏时在 293F culture media里面加了2%的 FBS， 复苏
后很好， 但可能是因为加入 FBS的原因，大部分细胞贴壁了。 随后的培养中去掉了
FBS， 活着的细胞还是继续贴壁，游离的细胞都是死细胞 或者 不是很健康。 而且可
能是因为没有 在orbital shaker里面培养的原因， 有很多细胞团。
问一下论坛里面的前辈， 你们培养293F细胞时有什么需要注意的事项么？ 非常感谢你
们的建议：） |
|
j*****d
发帖数: 787 | 41 http://www.nature.com/nature/supplements/collections/npgpublica
Page 4-5
Three reasons for success
Fuchu He believes that there are several reasons
why AMMS is able to deliver on its promises
with such high efficiency. The first reason
is quality. He says that in any country, ‘military
grade’ is always the highest grade because the
product must be able to operate under extreme
conditions. “Coartem and our independently
developed antiviral drugs reflect our quality and
capability,” He says.
The s... 阅读全帖 |
|
