a***n
发帖数: 71 | 1 以前没搞过hypoxia cell culture, 不知道哪家公司产品比较好，求有经验的同学推荐
下，包子答谢！ |
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k***g
发帖数: 4904 | 2 另外，实验室也有保鲜膜，那东西也可以用来密封culture dish吗？与parafilm有啥功
能上的区别么？比如透气性啥的？ |
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J*R
发帖数: 187 | 3 请教一下mouse embryo tissue culture需要用什么样的装置，大概要多少钱呢？买老
鼠的话平均1只大概要多少钱呢？谢谢～～ |
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t******k
发帖数: 599 | 4 那么问题来了，
如果做的是primary cell culture，n＝3是不是就应该杀三批老鼠？ |
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n***w
发帖数: 2405 | 5 this is a really good article.
the take-home msg here is to replicate at least times, at different times,
regardless of cell line or primary cultures. |
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t******k
发帖数: 599 | 6 那么问题来了，
如果做的是primary cell culture，n＝3是不是就应该杀三批老鼠？ |
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n***w
发帖数: 2405 | 7 this is a really good article.
the take-home msg here is to replicate at least times, at different times,
regardless of cell line or primary cultures. |
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w*e
发帖数: 740 | 8 各位养细胞的高手们，谁能推荐一种8 well的culture well plate？
我想在well里面染色，然后可以把well去掉，然后在上面放上cover slip的那种，如果
是poly lysine处理过的底面就最好了
谢谢 |
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s*****n
发帖数: 64 | 10 We made an extract from human tissue products with DMEM/10%FBS and added it
to human fibroblast. Initially, the cells attached to the plate though they
looked crappy. overnight, they all rounded up and died.
Luminex mulplex analysis showed the extract contained ~300 pg/mL IL-1a,6, 8,
600 pg/mL MCP-1, 800 pg/mL TGF-beta 1, 2 and 2 ng/mL PDGF-AA,BB.
Has anyone heard of cytokine storm in cell culture? |
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A******y
发帖数: 2041 | 11 Where do you get the frozen culture? OPM2 grow like weed in my lab. |
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r******k
发帖数: 446 | 12 最近小弟要culture K562. 以前没杨过悬浮的细胞。大神们给点意见？
1. 复苏以后怎么又贴壁细胞？？？
2. 怎么传代呀。。。离心下来数细胞数？ 那传到25cm dish 和T75的dish都需要seed
多少细胞？ 多久算养好？
谢谢 |
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A******y
发帖数: 2041 | 13 1. It will not attach to the plate. If you want the cells to attach to the
plate for immunostaining, you have to using fibronectin or matrigel etc.
2. The split is cells/mL. K562 should be sub-cultured at 200k/mL and no
more than 1 million/mL. However, it is very hard to kill K562 cells, so
even 1.5 to 2 million cells are possible. |
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a**i
发帖数: 289 | 14 【 以下文字转载自 Salon 讨论区,原文如下 】
发信人: akai (阿开), 信区: Salon
标 题: Why there was Great Culture Revolution?
发信站: Unknown Space - 未名空间 (Thu Jan 15 08:20:01 2004) WWW-POST
In fifties, sixties, and early seventies, China was in
an isolated and alone state. In international lever,
China was not in American, nor in Soviet ring, which
means China would not get support from either side.
But why did China do so? Why did Chinese leaders at
that time (Mao, Zhou and etc.) not just follow Soviet's
lead?
Because Chin |
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t****u
发帖数: 8 | 15 I am interviewing these days and begin to feel that there is a serious
cultural problem for Chinese students (i.e. attended undergraduate in China)
to win in the interviews. An interviewer chose a American Korean girl over me
simply because she chatted about sports in the interview and made the
interviewer laugh. I was so pissed off coz I have no idea about sports and am
not interested at all. Same thing with most things that Americans like, music,
movie stars or whatever. Do you guys think that |
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t****u
发帖数: 8 | 16 U-turn, thanks a lot for the comments. I realize the importance of this whole
culture thing but find it very hard to fix. Sometimes I feel that I am simply
not interested. I know that this is a bad atitude, since we all want to be
lawyers and everything, maybe it's just me. In law schhol, sometimes I will
sit next to a person and don't talk at all, not because I don't want to, but
just because I don't know about to talk about except for stuff of school. I
used to think that law school is a good |
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a***a
发帖数: 49 | 17 You know, the whole cultural thing might have played a role in your interview,
but that could not have been the whole story. There will be other opportunities.
Do not worry! You prepared for all these before applying for law school, did
you? |
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g****n
发帖数: 124 | 18 yeah..
when I was taking metro trains to an informational interview
I was thinking about
the cultural things at the same time
my conclusion is kinda like
I don't need to really know a lot about sports I don't like
what's important is being in a good mood
and always being able to bring up good topics
or keep asking questions
and it worked!
thanks for what you said too |
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T*******O
发帖数: 179 | 19 个人意见：
1， 上一些ESL，或者是关于american culture的课程，理论上了解中美文化差异
2， 需要多与美国人接触，观察他们的言谈举止，这可能要日积月累。 |
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k**7
发帖数: 20 | 20 最低目标: 至少共建和谐.
来美中国人多数在那些高科技公司工作.我们这里做护士的不占多数.(非法移民除外).
我个人感觉那些公司的人多数教育背景比较高,不少很国际化.所以面对这种barrier比
起在医院工作的我们容易对付些.
东西两岸少数民族众多,其实对我们来说比较有利.可是很多州医院护士还是以白人为主
.记不清哪本书上统计:全美护士caucasian占90%+.
我个人:上学全班只我一亚洲人.其它全白.和同学除课堂外没什么有效的交流.大家兴趣
实在不同,没感觉.
工作:我所在科室除我外,全白.我的工作接近casual,又是实习生,和同事相处也是面熟,
聊起天没共同语言,很难溶进去.
所以,如果正式工作了,同事都是清一色白人的话,如果还这样,对前途还是有影响的.因
为人际关系哪都存在.如果我们形单影之,很容易被忽略.(工作在ethnic diversity的地
方应该好很多)
美国人的文化是非常aggressive的.我们中国长大的人很难也做到和他们一样的
aggrssive.
language barrier:英语再好,俚语我们是不明白的.口音也影响人和人的距离.
Culture: |
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t*****e
发帖数: 2228 | 22 听过一个ms的presentation,里面有好多ms自己人创作的关于finance得搞笑图，
非常impressive,ms 的墙上还贴了很多剪报，都是关于ms历史的，觉得很有culture,
相反的gs什么也没感觉，里面的人都非常aggressive,字典里没有nice这个词//escape |
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l*****n
发帖数: 58 | 23 For bathroom sink vanity top, which material is better, cultured marble or
actual marble?
I need a 45'' and 53'' vanity tops, they are heavy for me to install without
help if the material is actual marble.
I already install a standard 31'' vanity top |
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t***r
发帖数: 294 | 24 Seems cultured marble doesn't need seal. but their size are limited, i
believe there is more choices for actual marble. for 45'' and 53'' vanity
tops, its not that heavy, you just need one more person to lift it up a few
times to install it. I have done a 48" counter-top.
Ask the granite retailer to cut it to your spec and grind it for you, which
should not cost much. Then you can just install it.
without |
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l*****n
发帖数: 58 | 25 Then cultured marble is a btter choice for people, who don't like/forget to
do seal annually. |
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W******e
发帖数: 745 | 26 我现在想买一个package来做IVF，虽然贵一点，但是不成功的话，可以随时退出，他们
退70%的钱。
这个package cover以下两个项目中的一个：Assisted Hatching or Blastocyst
culture。如果两个都做的话，自己要交钱cover其中的一个。
大家看看，哪个更重要，哪个更贵？谢谢！ |
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a*******l
发帖数: 116 | 27 我的理解是，assisted hatching一般是岁数大一些的建议做，这里很多jms移植的是
day 5的blastocyst。但是这2个都是要听医生建议的，尤其blastocyst culture,我觉
得不是自己选择的，是医生根据当时embryo的情况决定做到什么stage.这就是医生一般
在ER以后并不能马上告诉你是第三天还是第五天ET,因为要看embryo的发展情况。
价钱方面不了解。 |
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h*******d
发帖数: 1191 | 28 如果是我,我选blastocyst culture.因为如果你的受精卵能到blastocyst的话就说明卵
质量很好成功的希望就很大,我上次尽管做了AH还是失败了,因为卵的质量不好. |
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g******y
发帖数: 2517 | 32 14k Yellow Gold 6-6.5mm A-Grade Akoya Cultured Pearl Necklace, 18", 74% Off
http://amzn.to/zAczFR |
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a**i
发帖数: 289 | 33 【 以下文字转载自 Salon 讨论区,原文如下 】
发信人: akai (阿开), 信区: Salon
标 题: Why there was Great Culture Revolution?
发信站: Unknown Space - 未名空间 (Thu Jan 15 08:20:01 2004) WWW-POST
In fifties, sixties, and early seventies, China was in
an isolated and alone state. In international lever,
China was not in American, nor in Soviet ring, which
means China would not get support from either side.
But why did China do so? Why did Chinese leaders at
that time (Mao, Zhou and etc.) not just follow Soviet's
lead?
Because Chin |
|
v******a
发帖数: 45075 | 34 A CULTURAL STUDY OF THE AMERICAN EAST ASIAN POLICY
ZHU Shida
I
East Asia, driven by the Chinese huge market with a 1.2 billion
population
and the economic dynamism with a doubledigit growth, is becoming the growth
c
e
nter of the world economy. The growth rates of the East Asian countries
averaged
8% during the past twenty years, while the average growth rate of the
developed
nations registered 3%. The average growth rate of South Korea, Taiwan, Hong
Kon
g and Singapore — the “Four Dragons |
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g****y
发帖数: 323 | 35 今天在slashdot.org上面读到一篇关于不丹(Bhutan)的文章。
不丹最后一个将电视合法化的佛教国家。紧接着而来的是巨大的变化。
http://www.guardian.co.uk/weekend/story/0,3605,975769,00.html
上面这篇报道引发了在 slashdot.org 上的讨论. 讨论的中心就是 cluture.
其中有些评论很有意思.
by SerpentMage (13390) on Thursday August 08,
@05:25AM (#4031476) http://www.devspace.com/
I think the problem with American culture is that it tends suck you in and
forget about the others.
Let me give you an example. In the US there is a wine company that sells
itself a |
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s*****n
发帖数: 430 | 36 A&E
Biography TV
这个礼拜猪蹄：New Culture Icons，每天晚上8pm EST
昨天是Jerry Seinfeld，今天是Madonna
Jerry的女儿比他的床要好看多了，
更不用说他那个三千五百万的难看的房子。
不过下次去the City该去看Jerry的stand up。
Madonna当然没有出来被interview。
http://www.biography.com/icons/ |
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D*********n
发帖数: 279 | 37 http://en.wikipedia.org/wiki/Native_Americans_in_the_United_Sta
Native Americans in the United States
From Wikipedia, the free encyclopedia
Jump to: navigation, search
"American Indian" redirects here. For other indigenous peoples, see
Indigenous peoples of the Americas and other geographic regions. For
Americans from South Asia, see Indian American.
This article may be too long to read and navigate comfortably. Please
consider splitting content into sub-articles and/or condensing it. (January
2... 阅读全帖 |
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d*******d
发帖数: 3382 | 38 The 'Tiger Mom' Superiority Complex
A new book from Amy Chua and Jed Rubenfeld seeks to explain why some groups
succeed in America, and some fail. But when does cultural pride cross over
into racism?
By Suketu Mehta Monday, Feb. 03, 2014
From time to time, every Indian American finds an email in his or her inbox,
wearing a font of many colors, like the one my grandfather once sent me: "
Take a Pride--Being an Indian. 38% of Doctors in U.S.A. are Indians. 36% of
NASA employees are Indians. 34% of... 阅读全帖 |
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c***z
发帖数: 6348 | 39 节选，关于culture
Workplace cultures
I mentioned rank and tough cultures above, so let me get into more detail of
what those are. In general, an organization is going to evaluate its
individuals based on three core traits:
subordinacy: does this person put the goals of the organization (or, at
least, his immediate team and supervisor) above her own?
dedication: will she do unpleasant work, or large amounts of work, in order
to succeed?
strategy: does she know what is worth working on, and direct her ... 阅读全帖 |
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c***z
发帖数: 6348 | 40 节选，关于culture
Workplace cultures
I mentioned rank and tough cultures above, so let me get into more detail of
what those are. In general, an organization is going to evaluate its
individuals based on three core traits:
subordinacy: does this person put the goals of the organization (or, at
least, his immediate team and supervisor) above her own?
dedication: will she do unpleasant work, or large amounts of work, in order
to succeed?
strategy: does she know what is worth working on, and direct her ... 阅读全帖 |
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z****z
发帖数: 2054 | 41 对了解alt-right应该有帮助.
An Establishment Conservative’s Guide To The Alt-Right
by Allum Bokhari & Milo Yiannopoulos29 Mar 20164584
A specter is haunting the dinner parties, fundraisers and think-tanks of the
Establishment: the specter of the “alternative right.” Young, creative
and eager to commit secular heresies, they have become public enemy number
one to beltway conservatives — more hated, even, than Democrats or loopy
progressives.
The alternative right, more commonly known as the alt-right, is ... 阅读全帖 |
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p********1
发帖数: 2785 | 42 这是Amazon上Lin的评论。存在这里做个纪念：
As a Chinese, I lived through that period of time in China. I have similar
family and educational background as hers and suffered during Culture
Revolution as a child. I think her experiences in China mostly, if not all,
are fabricated, imagined, overly exaggerated or deliberately miss leading.
If one just read the media reports about her book, he/she may think that
media might just pick the sensational parts of the book and exaggerated a
little bit to help her to sell ... 阅读全帖 |
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J*****w
发帖数: 180 | 43 我朋友正在帮国内厂家物色有领导能力的，有创业精神的专家，在国内药厂组建蛋白质
药物开发部。
A leading China Pharma Company has immediate openings for the following four
leadership positions in its Yantai, China and Singapore sites. Please send
your resume to [email protected] if interested.
Title: Head, Upstream Process Development
Functional Description:
Lead, build and develop a high performance Upstream Process Development
group engaged in mammalian cell culture development for commercial
therapeutic proteins, including subclone/clone sel... 阅读全帖 |
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r**i
发帖数: 2328 | 45 今天特意休假一天，为一年一度的班级Chinese New Year Party做Volunteer。上来给
大家汇报一下。
先去做CLASSROOM DECORATION，挂起两个大红灯笼。我全副唐装上阵，班上中国小孩全
部唐装。人员三个妈妈，一个爸爸（摄影师）。
主要活动是包蔬菜馅儿的馄饨。妈妈们事先准备好材料，每桌发好馄饨皮，装馅儿的色
拉盆，摊馄饨皮的盘子，清水，湿纸巾，勺子等等。一妈妈给演示如何包馄饨，然后孩
子动手包馄饨。一年级大的孩子动手能力很强，有几桌20分钟内包完50个馄饨。很多孩
子非常CREATIVE，创造自己的FUSHION，什么BURITTO WONTON，SANDWITCH WONTON，包
子馄饨之类。我们几个家长狠狠夸奖了有创意的包法。
包完了，两妈妈煮馄饨，我负责介绍中国新年的习俗。大概讲了有20分钟。我准备了三
个问题，八页图片，和两个玩具。三个问题是什么是CULTURE，为什么学习不同的
CULTURE是重要的，通过学习中国CULTURE你你能得到什么。八页图片介绍了中国的”福
“字，生肖，年画，中国孩子怎样过新年，舞龙舞狮游行，烟花爆竹，大红灯笼，... 阅读全帖 |
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r**i
发帖数: 2328 | 46 【 以下文字转载自 Parenting 讨论区 】
发信人: raki (Sela\'ma ashal\'anore!), 信区: Parenting
标 题: 新年活动归来汇报（附材料）
发信站: BBS 未名空间站 (Fri Jan 31 15:17:15 2014, 美东)
今天特意休假一天，为一年一度的班级Chinese New Year Party做Volunteer。上来给
大家汇报一下。
先去做CLASSROOM DECORATION，挂起两个大红灯笼。我全副唐装上阵，班上中国小孩全
部唐装。人员三个妈妈，一个爸爸（摄影师）。
主要活动是包蔬菜馅儿的馄饨。妈妈们事先准备好材料，每桌发好馄饨皮，装馅儿的色
拉盆，摊馄饨皮的盘子，清水，湿纸巾，勺子等等。一妈妈给演示如何包馄饨，然后孩
子动手包馄饨。一年级大的孩子动手能力很强，有几桌20分钟内包完50个馄饨。很多孩
子非常CREATIVE，创造自己的FUSHION，什么BURITTO WONTON，SANDWITCH WONTON，包
子馄饨之类。我们几个家长狠狠夸奖了有创意的包法。
包完了，两妈妈煮馄饨，我负责介绍中国新年... 阅读全帖 |
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H****g
发帖数: 14447 | 47 1. 证实了毛泽东1975年写给周恩来这首诗。
《江山靠谁守·诉衷情》
当年忠贞为国筹，
何曾怕断头？
如今天下红遍，
江山靠谁守？
业未竟，
身躯倦，
鬓已秋。
你我之辈，
忍将夙愿，
付与东流？
2. 证实了1976年之前根本没有所谓饿死三千万或者大饥荒之说。
3. 毛泽东1975年的担心，或者说1964年的担心，都变成了现实。
4. 毛泽东唯一的遗产就是留给人民的造反精神，终将成燎原之火。
Mao Tse-Tung: Father of Chinese Revolution
By Fox Butterfield
Special to The New York Times
HONG KONG, Sept. 9--Mao Tse-tung, who began as an obscure peasant, died one
of history's great revolutionary figures.
Born at a time when China was wracked by civil strife, beset with terrible
poverty and enc... 阅读全帖 |
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m*******e
发帖数: 1598 | 48 BY TINA AMIRTHA8 MINUTE READ
This story contains interviews with Christian Kästner, assistant
professor at Carnegie Mellon University; Amy Hirotaka, state policy and
advocacy manager at Code.org; and Janet Siegmund, researcher at the
University of Passau.
In order to expose more students to computer science, 17 states have passed
legislation to create basic math and science requirements in the curriculum,
rather than count them as electives. But lawmakers in Texas have made
computer program... 阅读全帖 |
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