m***c
发帖数: 14 | 1 你们有没有看明白我说的意思啊？dendritic是懂生物的吧。据我所知，疫苗可以是低活
或是灭活的病原菌。把疫苗注射到体内能刺激免疫系统对这种病原的免疫力。那么这篇文
章可以被看作一种疫苗，它的目的是使中国人清醒意识到病原的危害性，产生强有力的机
制对付它。我并没否定应该自强呀。
写
考
条
国
而
合 |
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A***l
发帖数: 302 | 2 4. Multiple antigen peptide (MAP) approach
In order to avoid possible undesirable side effects of adjuvants/carriers,
another approach, MAP has been applied to the development of synthetic
vaccines. The goal of it is to design chemically unambiguous, multivalent
vaccines containing the relevant B- and T-cell epitopes but requiring no
adjuvants/carriers. This approach uses a small peptidyl core matrix bearing
radially branching synthetic peptides as dendritic arms. The simplest
design is to util |
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i*****r
发帖数: 65 | 3 RESEARCH ARTICLES
Local and Long-Range Reciprocal Regulation of cAMP and cGMP in
Axon/Dendrite Formation
Maya Shelly,1,* Byung Kook Lim,1,* Laura Cancedda,1,2 Sarah C.
Heilshorn,1, Hongfeng Gao,1 Mu-ming Poo1,
Cytosolic cyclic adenosine monophosphate (cAMP) and cyclic guanosine
monophosphate (cGMP) often mediate antagonistic cellular actions of
extracellular factors, from the regulation of ion channels to cell
volume control and axon guidance. We found that localized cAMP and cGMP
activities in |
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b******d
发帖数: 1 | 4 Post for a friend. Please contact the PI directly. Thanks.
1.position one:
Recruitment of a co-Principal Investigator at Prof. Yongjun Liu’s
Laboratory in Institut Pasteur of Shanghai, CAS
Prof. Yongjun Liu’s laboratory in IPS, CAS focus on 1) Dendritic Cell
Biology in anti-viral immunity; 2) Characterization of DNA/RNA sensors
within the innate immune system; 3) interaction between hepatitis C virus
and human immune system. For more information, please visit:
http://www.shanghaipasteur.cas.cn/k |
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c*********t
发帖数: 340 | 5 Dendreon
They isolate dendritic cells from the patient and stimulate with PAP (
prostate associated phosphate or something, a tumor associated antigen), and
infuse back
the median survival time could be prolonged by four months
then tumor cells expressing low levels of PAP would come up
75K for one round of treatment
really expensive
but it works |
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w***t
发帖数: 303 | 6 感觉类似的工作很多的样子，这个基因那个蛋白的，要么就是整个疾病模型
N-cadherin-dependent neuron–neuron interaction is required for the
maintenance of activity-induced dendrite growth
Zhu-Jun Tana,b,1, Yun Penga,b,1, He-Ling Songa, Jing-Jing Zhenga,b, and
Xiang Yua,2
+ Author Affiliations
aInstitute of Neuroscience and State Key Laboratory of Neuroscience,
Shanghai Institutes for Biological Sciences, and
bGraduate School of the Chinese Academy of Sciences, Chinese Academy of
Sciences, Shanghai 200031, China
Edited* by Charles F. |
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d******r
发帖数: 124 | 7 顶一下。
之前考虑过Tau-1，但后来看到paper说，在culture里，tau1也会染dendrite 和 cell
body
是不是没有特别特异性的axon marker？ |
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o**i
发帖数: 1165 | 11 纯化蛋白一般都加DTT吧
防止非特异cys bonding? |
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o*****n
发帖数: 445 | 12 如果都加dtt,需要形成双硫键的蛋白就不能正常fold了？ |
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n********k
发帖数: 2818 | 13 Joke of the day or what?
there is not a most shameless but more shameless!
Results: 33
1.
Viable fertile mice generated from fully pluripotent iPS cells derived from
adult somatic cells.
Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Wang X, Wang L, Zeng F,
Zhou Q.
Stem Cell Rev. 2010 Sep;6(3):390-7.PMID: 20549390 [PubMed - in process]
Related citations
2.
Production of mice using iPS cells and tetraploid complementation.
Zhao XY, Lv Z, Li W, Zeng F, Zhou Q.
Nat Protoc. 2010;5(5):963-71. Epu... 阅读全帖 |
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l***d
发帖数: 1828 | 14 http://www.livescience.com/culture/brain-3d-map-copy-cat-101115.html
Chinese team's brain imaging device has come under question from developers
of a U.S. device who say it's a near duplicate of theirs, LiveScience has
learned. An article on the Chinese device was published in the prestigious
journal Science, and the U.S. researchers are preparing a formal letter to
the journal in response.
According to the report appearing in the Nov. 4 online edition of Science,
the Chinese imaging device used... 阅读全帖 |
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l*****m
发帖数: 122 | 15 最近需要在老鼠里杀掉DC看看 但是又不能用DTR来做 想看看有没有直接用antibody
deplete DC的应用 我查到的基本都是in vitro条件的 想问问大家有没有谁做过in
vivo的呀？或者有推荐文献也行 谢谢啦！ |
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s****9
发帖数: 932 | 16 It is unlikely to use antibody to deplete DC in vivo. |
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s****9
发帖数: 932 | 19 Another alternative to consider is Flt3-ligand blockade. It remarkably
reduces DCs numbers, but you need to check more reference to see whether it
also influence some other myeloid compartments. |
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p****l
发帖数: 291 | 20 in vivo不都是用
Steffen Jung或者Guenter hammerling的老鼠吗
你不能用DTR为啥？ |
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p****l
发帖数: 291 | 21 Flt3L不能deplete掉DCs，当然可以显著减少DCs数量
it |
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l**********n
发帖数: 240 | 22 Josef Martin Penninger
Most significant scientific contributions:
Our basic approach is to genetically manipulate and change genes in mice and
to
determine the effects of these mutations in development of the whole
organism and in
diseases. From these mutations we are trying to establish basic principles
of
development and basic mechanisms of disease pathogenesis. My laboratory
focuses on
heart diseases, autoimmune diseases and cancers, and bone diseases. On all
the listed
contributions below I ... 阅读全帖 |
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U******u
发帖数: 5829 | 23 撤稿造假第一名是印度，中国位居第二
http://www.dxy.cn/cms/upload/2010/retraction/index.html
有人的地方就有江湖，有论文的地方就有Retraction。在这个撤稿稀疏平常的年代，你
再淡定也好，雷点再高也罢，生活中总还是有那么一些人，那么一些事牵动着我们自以
为不再敏感的神经。Retraction Watch最近就爆出这么一条新撤稿消息，称“几乎所有
能被企图欺诈用来篡改论文的都被这个来自中国的造假团队做到了”此条消息发布数小
时便受到上万人的关注，有些人再也淡定不起来。
事 件 回 放
被撤稿之曾经荣耀皆乌云
2010.11.25丁香园网友在站内爆料，Retraction Watch最近爆出新撤稿消息：In July,
the editors of Cancer Biology & Therapy published a retraction remarkable
for its scope. Apparently, nearly everything dishonest authors can do to
doctor a ma... 阅读全帖 |
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n***3
发帖数: 663 | 24 Adv Exp Med Biol. 2007;601:155-62.
Immunological role of dendritic cells in cervical cancer.
Manickam A, Sivanandham M, Tourkova IL
please send the paper to e*********[email protected]
Thanks a lot! |
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b********i
发帖数: 73 | 26 Communicable ulcerative colitis induced by T-bet deficiency in the innate
immune system.
Garrett WS, Lord GM, Punit S, Lugo-Villarino G, Mazmanian SK, Ito S,
Glickman JN, Glimcher LH.
blabla..... T-bet deficiency in the innate immune system results in
spontaneous and communicable ulcerative colitis in the absence of adaptive
immunity and increased susceptibility to colitis in immunologically intact
hosts. T-bet controls the response of the mucosal immune system to
commensal bacteria by regulati... 阅读全帖 |
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m**i
发帖数: 355 | 27 Immunotherapy. 2011 Apr;3(4 Suppl):19-20.
Regulatory B cells control dendritic cell functions.
Lo-Man R.
多谢了！ 我的email地址是：x*******[email protected] |
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w********h
发帖数: 12367 | 28 The 2011 Nobel Prize in Physiology or Medicine was divided, one half jointly
to Bruce A. Beutler and Jules A. Hoffmann "for their discoveries concerning
the activation of innate immunity" and the other half to Ralph M. Steinman
"for his discovery of the dendritic cell and its role in adaptive immunity". |
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w********h
发帖数: 12367 | 29 The 2011 Nobel Prize in Physiology or Medicine was divided, one half jointly
to Bruce A. Beutler and Jules A. Hoffmann "for their discoveries concerning
the activation of innate immunity" and the other half to Ralph M. Steinman
"for his discovery of the dendritic cell and its role in adaptive immunity". |
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l****t
发帖数: 102 | 30 The 2011 Nobel Prize in Physiology or Medicine was divided, one half jointly
to Bruce A. Beutler and Jules A. Hoffmann "for their discoveries concerning
the activation of innate immunity" and the other half to Ralph M. Steinman
"for his discovery of the dendritic cell and its role in adaptive immunity". |
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i***R
发帖数: 663 | 31 UT Southwestern Scientist Shares 2011 Nobel Prize in Physiology or Medicine
Dr. Bruce A. Beutler, the new Director of the Center for the Genetics of
Host Defense at UT Southwestern Medical Center, today shared the Nobel Prize
in Physiology or Medicine with two other scientists for their discoveries
in how the immune system works.
Dr. Beutler and Dr. Jules A. Hoffmann of Strasbourg University’s Institut
de Biologie Moléculaire et Cellulaire in France shared half the prize for
their discovery of r... 阅读全帖 |
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h*******n
发帖数: 2052 | 32 Prof Steinman was being treated with a dendritic-cell based therapy which he
designed himself. |
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R***t
发帖数: 14 | 33 代友发帖
～～～
Postdoctoral Research Fellow in Cancer Immunology, Medical University of
South Carolina, Charleston, SC, United States
Postdoctoral position is available in an exciting new laboratory to study
cancer immunology.
My lab is interested in cancer vaccine and innate immunity. My prior work
has been in the area of defining the roles of heat shock protein gp96 in
chaperoning TLRs (Liu et al. Nature Commun, 2010), understanding the roles
of gp96 in regulating hematopoiesis (Liu and Li, Blood 20... 阅读全帖 |
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w******n
发帖数: 371 | 34 标 题: 第一批CLS博士后基金入选者名单公示（清华博士后水准高）
发信站: 水木社区 (Sun Nov 27 11:44:48 2011), 站内
八位申请人拟入选CLS清华博士后基金，现将名单公示如下：
特等博士后基金：
1.龚蓉：2011年7月，在中国医学科学院北京协和医学院获得理学博士学位
2005年7月， 在武汉大学获得理学学士学位
代表性一作论文：
1.Rong Gong*, Ji Hu* Cheng Ding* et al. (2011). Role for the membrane
receptor guanylyl cyclase-C in attention deficiency and hyperactive behavior
. Science 333:1642-1646. (*equal authorship.)
2、梅子青：2009年12月，在清华大学获得理学博士学位
2002年7月， 在吉林大学获得理学硕士学位
1999年7月， 在吉林大学获得理学学士学位
... 阅读全帖 |
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D*******8
发帖数: 4 | 35 Please don’t dig me out. I am writing this out of my conscience as a
scientist. We are using, largely, the tax payers’ money to explore
mechanisms underlying diseases that we don’t have a cure, yet. We are doing
this in the hope that little bit knowledge can accumulate till one day we
can find that magic bullet. However, there are people out there, their
purpose is to use these money to achieve their personal fames, sometimes
international ones, totally disregarding our original noble cause. I... 阅读全帖 |
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P****o
发帖数: 172 | 36 I know very little about other fileds. But in immunology field, Liu is a
well-known top scientist, who did great contribution in the B cell field at
his earlier career stage.Of course, his most famous work is the discovery of
plasmacytoid dendritic cells, which play a central role in anti-viral
immunity. It was said that his H-index is 78.
We have a bunch of good immunologists in U.S.A, but Liu should be the one
with most prominent reputation.
http://www.inboxgroup.net/baylorri/e_article00208784... 阅读全帖 |
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l**********1
发帖数: 5204 | 38 //webdoc.nyumc.org/nyumc/files/sun-lab/attachments/CPCB.ch02.Isolate%20cells
.pdf |
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c********n
发帖数: 67 | 41 Simultaneous imaging of morphological plasticity and calcium dynamics in
dendrites
Thanks a lot for your kindly help
h***[email protected] |
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x*****a
发帖数: 972 | 42 CD31在macrophage也有大量表达。如果看Murine macrophage，F4/80是个比较接受的
marker，但是F4/80在有些dendritic cell或者monocyte上也有表达，所以F4/80是个不
完全纯粹的marker。
CD34是个stem cell/progenitor cell的marker，如果是非常成熟的endothelial cells
，是不太表达这个的，你染出来的可能是较为后期的endothelial progenitor cell。
其实vWF就是最好的了，既然染出来了，就可以了啊。VE那个当然也行。 |
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m*****l
发帖数: 28 | 43 Publication 惨不忍睹， Pubmed: Gray NW
3篇first－author文章。2005年的文章Figure 5还是错的，2003年的文章Figure 3 ps
的不行。
不是这个领域的，看不出来造假没有。
谁要是找出来我给谁发包子。
奇怪了，这样的人怎么能当上Nature的editor去把握这个领域的最前沿。
A dynamin-3 spliced variant modulates the actin/cortactin-dependent
morphogenesis of dendritic spines.
Gray NW, Kruchten AE, Chen J, McNiven MA.
J Cell Sci. 2005 Mar 15;118(Pt 6):1279-90. Epub 2005 Mar 1.
Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5
and Homer.
Gray NW, Fourgeaud L, Huang B, Chen J, C... 阅读全帖 |
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p**t
发帖数: 160 | 44 Please read this paper, you are misleading others.
-----------J Innate Immun. 2012;4(5-6):424-36. doi: 10.1159/000337007. Epub
2012 Apr 4.
Lymph node macrophages.
Gray EE, Cyster JG.
Source
Howard Hughes Medical Institute and Department of Microbiology and
Immunology, University of California San Francisco, San Francisco, CA 94143-
0414, USA.
Abstract
Lymph node (LN) macrophages have long been known for their efficient uptake
of lymph-borne antigens. A convergence of studies on innate and adapti... 阅读全帖 |
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l**********1
发帖数: 5204 | 45 苏州独墅湖 CSH lab Asia meetings
别说去开会 就说回国看父母
那里可以见到几乎你跑美国面试> 十个 labs 的PIs
COLD SPRING HARBOR ASIA CONFERENCES
Development, Function and Disease of Neural Circuits
Suzhou Dushu Lake Conference Center
October 21-25, 2013
Abstract Due Date: August 9, 2013
Organized by:
Barry Dickson, Research Institute of Molecular Pathology, Austria
Zhigang He, Harvard Medical School/Children's Hospital, Boston, USA
Hitoshi Okamoto, RIKEN Brain Science Institute, Japan
Yimin Zou, University of California at San Di... 阅读全帖 |
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h***a
发帖数: 145 | 47 Expert Rev Vaccines. 2012 Dec;11(12):1441-57. doi: 10.1586/erv.12.117.
The role of dendritic cells in innate and adaptive immunity to respiratory
syncytial virus, and implications for vaccine development.
Garg R, Shrivastava P, van Drunen Littel-van den Hurk S.
Source
VIDO-Intervac, University of Saskatchewan, Saskatoon, SK, S7N 5E3, Canada.
http://www.ncbi.nlm.nih.gov/pubmed/23252388
Thanks a lot in advance. |
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C********4
发帖数: 308 | 49 一个条件性细胞剔除模式小鼠的诞生 (2013-02-22 18:10:22)转载▼
标签： talen 创业 大鼠基因敲除 基因敲除 杂谈 分类： 个人随笔
对于基因敲除（gene knockout）小鼠大家并不陌生，比如我们想要研究一个基因
在体内的功能，我们就可以把这个基因从小鼠体内敲除掉。而有的时候我们除了关心一
个基因的功能外，还想知道表达这个基因的细胞在体内是干嘛的。比如，我们想知道B
细胞是干什么的，胰岛细胞是干什么的？我们可以从小鼠体内把这些细胞清除掉（也叫
细胞剔除，cell depletion），一看不能产生新抗体了，或是胰岛素没了，血糖升高器
官衰竭了，我们就可以恍然大悟说：“你看，胰岛细胞是产生胰岛素的，B细胞是产生
抗体的”。然后就可以发文章到CNS（Cell, Nature, Science）了。当然这些都被别人
发现过了，是我们没有赶上好时代呀。
那怎样才能在体内把一群细胞清除掉呢？方法可能很多，但也真不多。这里我讲一
个非常简单的方法，就是白喉毒素介导的细胞剔除。白喉毒素（Diphtheria toxin, 简
称DT）大... 阅读全帖 |
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p*****c
发帖数: 20445 | 50 Michael Rape, Ph.D.
University of California, Berkeley
Michael Rape’s love of science began in the basement of his parent’s house
, where he used to conduct rudimentary biochemistry experiments. Today, he
uses far more sophisticated methods to understand a complex process critical
to nearly all organisms: ubiquitylation. The term describes the attachment
of a regulatory protein called ubiquitin—named for its ubiquity—onto other
proteins. Ubiquitin tags communicate a wealth of information to the ... 阅读全帖 |
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