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全部话题 - 话题: depleted
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W*****B
发帖数: 4796
1
Here's how much the $1.537 billion Mega Millions winner will get to save
and spend
The $1.537 billion Mega Millions jackpot win marks the largest in the game&#
39;s history and the second-largest lottery prize ever.
The lump-sum option is $877.8 million. Even after federal taxation of 37
percent and South Carolina's 7 percent tax, the total take would be
roughly $491.7 million.
Applying the so-called 4 percent rule — considered a safe yearly withdrawal
rate — would mean about $19.6 million could... 阅读全帖

发帖数: 1
2
July: Japanese assault
The Japanese plan was for a two-pronged assault. The first attack would be
made by three regiments plus part of a fourth: the 71st and the 72nd
Infantry Regiment (23rd Division), a battalion of the 64th Infantry Regiment
and the 26th Infantry Regiment under Colonel Shinichiro Sumi (7th Infantry
Division). This force would advance across the Khalkin Gol, destroy Soviet
forces on Baintsagan Hill on the west bank, then make a left turn and
advance south to the Kawatama Bridge... 阅读全帖

发帖数: 1
d*********o
发帖数: 6388
4
http://www.washingtonpost.com/national/coronavirus-protective-gear-stockpile-depleted/2020/04/01/44d6592a-741f-11ea-ae50-7148009252e3_story.html
Protective gear in national stockpile is nearly depleted, DHS officials say
As coronavirus outbreak intensifies, the government’s emergency stockpile
of masks, gloves and other medical supplies is nearly exhausted.
washingtonpost.com
b*******r
发帖数: 6655
5
来自主题: USANews版 - 为什么没有人再提保护臭氧
2001年以前,depletion was accelerating.我也是信徒之一。不知您老当时在考什么
古。
2003,depletion may be slowing down,CFC production had been stopped in most
developed countries, but most developing countries, such as China, didn't
stop until 2007. Meanwhile existing stock continued to release to atmosphere
. ODS level continued to rise.
2007....not many people paid attention to this issue any longer. The rest of
them acknowledged 臭氧层是一个很复杂的问题。so we don't want to discuss it.
There are easier topics to fool around.

发帖数: 1
6
来自主题: USANews版 - 满嘴跑火车的川普
川普是个满嘴跑火车的家伙,讲话不经大脑思考。就如那个瑞典事件,前一晚看的电视
里提到了瑞典,第二天到他嘴里就变成了瑞典昨晚粗大事,结果闹了个国际大笑话。另
外一个类似事例是 NYT 说他从 11/14 以来就没跟习近平说过话,他立刻上贴说 &
ldquo;Fake news, failing NYT %x@#*#!~~ ...... 人家我昨晚才跟习大大进行了亲切
的对话”,可是他根本没闹清楚,NYT 那篇文章是发于几天之前,是在川习亲切
谈话之前!
反正这家伙是个混人,不能信他说的话。看看他在 CPAC 大会上又跑了多少列火车吧,
真的是惊呆宝宝:
Fact Check: Trump Blasts ‘Fake News’ and Repeats Inaccurate
Claims at CPAC
By LINDA QIU FEB. 24, 2017
http://static01.nyt.com/images/2017/02/25/us/25factcheck3/25factcheck3-
master768.jpg
President Trum... 阅读全帖
T**********e
发帖数: 29576
7
Trump thinks that exercising too much uses up the body’s ‘finite’ energy
President Trump reportedly eschews exercise because he believes it drains
the body’s “finite” energy resources, but experts say this argument is
flawed because the human body actually becomes stronger with exercise.
Trump’s views on exercise were mentioned in a New Yorker article this month
and in “Trump Revealed,” The Washington Post’s 2016 biography of the
president, which noted that Trump mostly gave up athletics after c... 阅读全帖
W*****B
发帖数: 4796
8
【 以下文字转载自 Military 讨论区 】
发信人: WCNMLGB (CCC), 信区: Military
标 题: 床铺对于身体健康和锻炼的“电池理论”
发信站: BBS 未名空间站 (Mon May 15 15:33:21 2017, 美东)
核心就是下面这段,人体能量如同电池,是有限度的,过早过度运动,到老了就耗尽不
够用了
After college, after Trump mostly gave up his personal athletic interests,
he came to view time spent playing sports as time wasted. Trump believed the
human body was like a battery, with a finite amount of energy, which
exercise only depleted. So he didn't work out. When he learned that John
O'Donnell, one of his top casino e... 阅读全帖
d*2
发帖数: 2053
9
来自主题: USANews版 - 欧洲没有人权!
https://www.nytimes.com/2017/07/03/world/europe/uk-trump-pope-francis-
charlie-gard.html
人家自己掏钱送美国治病也不行么?
LONDON — Charlie Gard, who turns 11 months old on Tuesday, was born with an
extremely rare genetic disease. He is blind and deaf, and he cannot breathe
or move on his own. He suffers from persistent epileptic seizures.
Now he is at the center of a global debate, drawing in the Vatican and
President Trump, over what medical treatment, if any, he is entitled to
receive, and who decides — his f... 阅读全帖
F***Q
发帖数: 6599
10
来自主题: Automobile版 - Common EV acronyms
for those of you who want to read more about electric cars, here are some
acronyms you may see often:
BEV:
battery electric vehicle - often known as a full electric car,
a car runs exclusively on a rechargeable battery. examples include
Nissan LEAF and Tesla Model S.
PHEV:
plug-in hybrid electric vehicle - a electric-gas hybrid car,
typically with a large capacity rechargeable battery. most of them
can run under a "Serial hybrid" mode, i.e. run on battery first,
when ... 阅读全帖
m***8
发帖数: 1786
11
来自主题: Automobile版 - Nissan Leaf真是极品啊
http://www.mychevroletvolt.com/chevy-volt-energy-usage-charging
此文作者实测差13%。leaf的75%对85%,也是这个数。
, I’ve been able to see that at home on a 240 volt circuit, it takes
roughly 11.9 KwH to Fully charge my Volt’s depleted battery. (the weather
here has been pretty consistent at around 50-60 F, so chances are – no
extra energy was used to heat or cool the battery)Compare this to my 110v
charging experience inthe public, and you’ll see that the total power usage
was 13.5 Kwh. Granted, I only have one ... 阅读全帖
F***Q
发帖数: 6599
12
来自主题: Automobile版 - Chevy Volt Gen2 specs
more technical details on Volt 2 (in comparison to Gen 1)
http://insideevs.com/2016-chevrolet-volt-q-volt-engineers/
-Electric heater for cabin will be more powerful but still resistive.
-3.6kW charging now instead of 3.3kW
-Price similar to current Volt
-Brake lights come on after a set rate of deceleration now
-ERDTT will still exist with configurable levels (temperature options
unknown at this time)
-EPA rated 50 mile all electric range
-EPA rated 41 mpg in charge sustaining mode
-5 seats (a... 阅读全帖
g***n
发帖数: 14250
13
来自主题: Automobile版 - 全电驱动的汽车将来能否流行
2016 Volt 汽油机也驱动轮子,其实 2015 以前的在速度高时也驱动轮子
http://www.greencarreports.com/news/1096942_2016-chevrolet-volt-powertrain-how-it-works-in-electric-hybrid-modes
But while GM hasn't explicitly said so, it's no longer as much of a range-
extended electric car (or "series hybrid").
The new 2016 Volt should be viewed as a more conventional plug-in hybrid,
with engine torque now being sent to the wheels through a mechanical
connection whenever the engine is on.
The 2011-2015 Volt has one motor that powers t... 阅读全帖
g***n
发帖数: 14250
14
来自主题: Automobile版 - 现在插电车有这么设计的吗?
那偶再贴一次。叫你们温习功课也不听
发信人: goFan (够范), 信区: Automobile
标 题: Re: 全电驱动的汽车将来能否流行
发信站: BBS 未名空间站 (Sat Oct 15 14:25:36 2016, 美东)
2016 Volt 汽油机也驱动轮子,其实 2015 以前的在速度高时也驱动轮子
http://www.greencarreports.com/news/1096942_2016-chevrolet-volt-powertrain-how-it-works-in-electric-hybrid-modes
But while GM hasn't explicitly said so, it's no longer as much of a range-
extended electric car (or "series hybrid").
The new 2016 Volt should be viewed as a more conventional plug-in hybrid,
with engine torque now being sent... 阅读全帖
w**********x
发帖数: 46
15
来自主题: BackHome版 - 转让 United $2000 travel certificate
Transfer United electronic travel certificate with face value of $2000.
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been reached. Please see below for the terms &
conditions. Asking for $1800. I can either email you th... 阅读全帖
o*****6
发帖数: 22
16
来自主题: BackHome版 - UA Electronic Travel Certificate $700
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
o*****6
发帖数: 22
17
来自主题: BackHome版 - UA Electronic Travel Certificate $700
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
o*****6
发帖数: 22
18
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
w**********x
发帖数: 46
19
来自主题: FleaMarket版 - 转让 United $2000 travel certificate
Transfer United electronic travel certificate with face value of $2000.
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been reached. Please see below for the terms &
conditions. Asking for $1800. I can either email you th... 阅读全帖
o*****6
发帖数: 22
20
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
o*****6
发帖数: 22
21
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
o*****6
发帖数: 22
22
来自主题: FleaMarket版 - UA Electronic Travel Certificate $700
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
o*****6
发帖数: 22
23
来自主题: FleaMarket版 - UA Electronic Travel Certificate $700
Transfer United electronic travel certificate with face value of $700.I am
asking for $600. I can either email you the pin code or book the ticket for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been re... 阅读全帖
q****g
发帖数: 1190
24
来自主题: FleaMarket版 - UA Electronic Travel Certificate 2X $150
Transfer United electronic travel certificate with face value of $150.两张
asking for $100 一张. I can either email you the pin code or book the ticket
for
you.
有需要的朋友,可以站内联系。
This certificate can be used on any United airlines flights. If the face
amount of this certificate exceeds the cost of the ticket for which it is
surrendered, any residual amount will be applied to the same PIN for use
toward another ticket until either the original issued amount is depleted or
the expiration date has been r... 阅读全帖
c******n
发帖数: 4965
25
来自主题: JobHunting版 - Inside Uber’s Engineering Struggles
刚爆出的料, uber 真是糙快猛的代表啊
https://www.theinformation.com/inside-ubers-engineering-struggles?unlock=
22c087&token=4fa8aaabbd314173f46a69e9c2b4db43226d3169
The Information
Research Topics
The Team
Our Subscribers
Welcome, John
Semil Shah shared this article from The Information with you. For access to
more exclusive news, interviews, analysis and subscriber-only events,
subscribe to The Information.
Subscribe Now
EXCLUSIVE
Inside Uber’s Engineering Struggles
Inside Uber’s Engineering Struggles
Uber CT... 阅读全帖
m***y
发帖数: 14763
26
来自主题: Living版 - Skylight漏水怎么办
Hoho, most leaks are self-sealing : )
We engineers have a theory about this. If there is a hole to a pool, no
matter how large the pool could be or how small this hole could be, it will
deplete the pool eventually ... (drum rolls)... unless this hole is designed
to deplete the pool. In that case, the hole always got clogged : )
p***a
发帖数: 78
27
要装的, 房子大客厅高其实也没多少空气, 一会儿就被抽完了。你看看下面这个文章:
http://ccbinnovations.com/content/8-about-make-up-air-negative-air-pressure-and-fresh-air-infiltration
A high CFM kitchen ventilation system can deplete the air supply inside your
home rapidly (fig.1) while exhausting your cooking surface. For example, a
600 CFM (cubic feet per minute) vent hood can deplete the air in a kitchen
with a 10ft ceiling that is 10ft wide and 10ft deep (length x width x depth
= cubic feet) in less than 2 minutes.
A residential s... 阅读全帖
f****o
发帖数: 2770
28
Patients with Glanzmann thrombasthenia who are bleeding require platelet
transfusion.
Because patients are likely to require multiple transfusions during their
lifetime, take care to avoid platelet alloimmunization.
Prevention is best accomplished by using leukocyte-depleted blood products.
Leukocyte depletion can be accomplished with mechanical filtration. Only
filtered blood products should be given.
Use of platelets matched via human leukocyte antigen (HLA) is a further
attempt to prevent pla... 阅读全帖
f****o
发帖数: 2770
29
Patients with Glanzmann thrombasthenia who are bleeding require platelet
transfusion.
Because patients are likely to require multiple transfusions during their
lifetime, take care to avoid platelet alloimmunization.
Prevention is best accomplished by using leukocyte-depleted blood products.
Leukocyte depletion can be accomplished with mechanical filtration. Only
filtered blood products should be given.
Use of platelets matched via human leukocyte antigen (HLA) is a further
attempt to prevent pla... 阅读全帖
d****z
发帖数: 9503
30
Overspending deplete wealth. Even if nation don't need accumulate wealth, it
cannot deplete the wealth forever.
f*******m
发帖数: 2
31
可惜了我交了这么多的SS/medicare 税。。。
b*******k
发帖数: 16989
32
medicare养了太多没用的懒人,没有才好。
social security也差不多
s*****c
发帖数: 753
33
预留?
If the available visa number for this year is depleted, then it is depleted.
And some people before "big flood" will stuck for another year.
Or there are more visa number available that a group of people after "big
flood" could get their green card 4-10 month after they submit 485.
g********x
发帖数: 4671
34
来自主题: Fitness版 - Cardio完了做力量练习合适吗?

这个是正解。
When you do Cardio, the body has 2 sources of energy to choose from.
Glycogen/glucose and/or FAT. When you do resistance training, the body
basically has 1 sources of energy to draw from... Glycogen/glucose.
If you do your cardio (not the warm up...but the extensive) before you do
resistance training and deplete the glucose before you even start
lifting...what happens? The body has to get more glucose...where? It can
make it from muscle!!!
If you do resistance training and then cardi... 阅读全帖
r********r
发帖数: 2912
35
反了吧。。。load四天得吃成大胖子了
一般的话如果周日比赛,那就前一个周日跑一个medium long消耗一下,接下来周一到
周三deplete,周四到周六loading就挺合适的
boston是周一比赛,所以我当时就多deplete了一天,从周五才开始load的
r********r
发帖数: 2912
36
来自主题: Running版 - Harrisburg Marathon Report
先报成绩,2:48:18,没PR,但是过程很满意。
上周纽约马拉松不幸在最后时刻夭折,其实我也并不是非常的沮丧,本来跑步也不是为
了比赛的。不过今年的计划是就春天和秋天各跑两个相隔不太远的马拉松,就差最后一
个完成不了总会觉得不甘心,所以上周六火速报了离我住的地方开车一个多小时的
Harrisburg马拉松。Harrisburg马拉松在大比赛密集的秋天举行,城市不大,又在PA中
部,原本规模很小,吸引不来多少参赛者。去年比赛overall第四名的成绩只有2:49,
可见水平之低。今年在纽马取消前大概只有1000人左右报了名,但是上周六周日过后,
报名人数迅速膨胀到接近2000,显然这其中绝大多数都是纽约马拉松的"cancelee"。赛
事组织者倒也是很热情地积极应对,临时多赶印出1000件tshirt,准备了更多的食物和
水,在起点和赛道上又放置了好多新的porta johns,从今天实际的比赛运行效果来看
组织者做的真是很不错。当然今年肯定跑249是不可能进前四的了,报名的时候我就预
料到了。反正对于我来说Harrisburg马拉松是我们州几个比较大的城市的马拉松里我唯
一一个来没去... 阅读全帖
b***i
发帖数: 10018
37
来自主题: Running版 - Carbo Loading 的疑问
最后三天增加carb intake的比重就可以了。我们都试过carb depletion,效果不错。
不过有人说不用carb depletion也行,更安全一些,我准备明年波马试一下。
m**t
发帖数: 1956
38
来自主题: Running版 - 读书笔记-Hansons训练法 2
long run
很有争议的就是不跑20miler,16miler就够了。
首先,作者说,1)jack daniels说了,单个long run不能超过周里程的25%-30%;2)
传奇体育生理学家Tim Noakes说了,75%-85%VO2Max 跑2个小时以上,会导致greatest
glycogen depletion。别的研究人员也有同样的结论。过量的glycogen depletion导致
需要很长时间,72小时以上,的恢复。
如果按照25-30%算,周里程55的,最长16.5mile,70周历程的,21mile。所以对50-
60mpw的训练计划,Hansons认为16mile就够了。另外按照8分的pace跑16mile,要2小时
8分钟,从时间上也够了。
其次,Hansons的训练计划,也使得完成20miler比较困难。50多mpw(beginner)的计划
,在后期常有这样的训练:
星期天 16mile
星期1 5mile easy
2 3x2mi at mp-10s pace, 800m recovery, 1-2mi warmup&cooldown
3 off
4... 阅读全帖
j***y
发帖数: 5098
39
☆─────────────────────────────────────☆
ironman2015 (1/2 ironman) 于 (Fri Sep 30 16:08:08 2011, 美东) 提到:
早饭是没时间吃了(不然吃完还要等一两小时才能跑)
☆─────────────────────────────────────☆
pager (阿土) 于 (Fri Sep 30 16:18:26 2011, 美东) 提到:
training mode: no

☆─────────────────────────────────────☆
qcc (我吃草故我牛) 于 (Fri Sep 30 16:20:01 2011, 美东) 提到:
我一般吃几颗巧克力糖出门,去年halloween的糖终于快吃完,不过马上又可以补充了

☆─────────────────────────────────────☆
pager (阿土) 于 (Fri Sep 30 16:21:06 2011, 美东) 提到:
BSO 年轻,可以要到糖。
☆───────... 阅读全帖
d******0
发帖数: 22800
40
看到了这一段,看来还是要多跑。
I’m getting muscle cramps. What should I do?
There are two main theories on muscle cramping. The first is that they are
due to neuromuscular fatigue and the second is that they are due to
electrolyte depletion and dehydration. The best thing to do for muscle
cramping is to try to prevent them in the first place. Nothing substitutes
for training.
Specifically trained muscles will be adapted to the tough conditions at WS.
Sometimes, however, cramps are unavoidable. So, if cramping ... 阅读全帖
C*****H
发帖数: 7927
41
☆─────────────────────────────────────☆
days (count+days) 于 (Sun Aug 18 23:10:02 2013, 美东) 提到:
Hi, All:
大家平时跑步时喝什么饮料 ?白水还是gatorade ? 还是白水加点盐 ?
如果跑半马, 白水是否不能够补充盐呢 ?
(今天跑步时,小腿有点抽筋, 所以有这个问题)。
Thank you
☆─────────────────────────────────────☆
xincheng (新城) 于 (Sun Aug 18 23:25:18 2013, 美东) 提到:
我一般跑得长喝运动饮料。短的话就随便喝点水就可以了。

☆─────────────────────────────────────☆
dash2000 (DASH) 于 (Mon Aug 19 00:11:01 2013, 美东) 提到:
我的答案一直是椰子水!呵呵。
☆─────────────────────────────────────☆
babymagic (我心依旧)... 阅读全帖
b*****d
发帖数: 61690
42
【 以下文字转载自 USANews 讨论区 】
发信人: TravisBickle (Travis), 信区: USANews
标 题: 床铺:锻炼太多损耗身体的元气
发信站: BBS 未名空间站 (Sun May 14 23:29:04 2017, 美东)
Trump thinks that exercising too much uses up the body’s ‘finite’ energy
President Trump reportedly eschews exercise because he believes it drains
the body’s “finite” energy resources, but experts say this argument is
flawed because the human body actually becomes stronger with exercise.
Trump’s views on exercise were mentioned in a New Yorker article this month
and ... 阅读全帖
l*****9
发帖数: 9501
43
来自主题: Piebridge版 - 女人的福音:60岁生娃不是梦了
http://www.businessweek.com/news/2012-02-27/ovarian-stem-cells-
Ovarian Stem Cells Make Human Eggs in Possible Aid to Fertility
By Ryan Flinn
Feb. 27 (Bloomberg) -- Stem cells taken from human ovaries were used to
produce early-stage eggs by scientists in Boston who may have created a new
method to help infertile women.
Females have a fixed number of eggs from birth that are depleted by the time
of menopause. The finding, published today in the journal Nature Medicine,
challenges the belief that... 阅读全帖
z*****i
发帖数: 941
44
来自主题: Jiangsu版 - Re: 美国很牛么
1. Demand for greater consumption is what drives supply. With the increasing
demand and supply for consumption, the economy can only move forward.
2. The problem with the depleting and depleted natural resources can be
compensated by the advancement of technology -- for recycling as well as a
better and more efficient use of existing resources.
If these two propositions hold, as Americans believe, the world will be o.k.,
and in fact better, even when all countries reach the level of consumption
z**r
发帖数: 17771
45
来自主题: EmergingNetworking版 - 1/31是一个值得纪念的日子
Today, IANA (Internet Assigned Numbers Authority) have officially handed out
the last remaining unallocated blocks of IPv4 addresses.
This is an important date and event in the history of the Internet. It also
means pace toward IPv6 migration will quicken as IPv4 address depletion at
the global level have taken place. Regional level depletion is projected to
be within next 6~9months depending on individual allocation rates and
policies.
s***e
发帖数: 911
46
Crowding的驱动力是depletion force. Depletion force本质上和Osmotic pressure的
起源一样, 都不是具体的物理相互作用力, 而是熵力. 换句话说靠局部的熵减换取全
局的熵增.
对前面说的DNA 例子而言, DNA紧密贴在一起(熵减), 可以省出一些水去dilute
crowding molecules (熵增). 对protein而言, 道理也是一样的. 不过我没有见过
crowding对蛋白folding影响的研究.
l**********1
发帖数: 5204
47
he/she can try stimulated-emission depletion (STED)
paper:
htp://www.ncbi.nlm.nih.gov/pubmed/16896340
>We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy
with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is
demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell
Brand:
Super-Resolution Microscope Leica TCS STED
htp://www.leica-microsystems.com/products/confoca... 阅读全帖
n********k
发帖数: 2818
48
来自主题: Biology版 - 从total RNA里面提取mRNA-求建议
do u want mRNA only or you want rRNA depletion, for rRNA depletion, rRNA-
zero from epicentre is the way to go...with one around, I got virtually no
rRNA as determined by agilent Chip...based on the kit, the starting RNA is about 5ug and ends up with 150-
400ng RNA. So about 3-8%...that's what I got for all my 8 samples...

total
l**********1
发帖数: 5204
49
sure
add one recent paper
Levy S at al. (2011)
The competitive advantage of a dual-transporter system.
Science. 334: 1408-12.
Abstract
Cells use transporters of different affinities to regulate nutrient influx.
When nutrients are depleted, low-affinity transporters are replaced by high-
affinity ones. High-affinity transporters are helpful when concentrations of
nutrients are low, but the advantage of reducing their abundance when
nutrients are abundant is less clear. When we eliminated such red... 阅读全帖
l**********1
发帖数: 5204
50
Please refer
Swedish Umeå Universitet. [2007] one PhD Dissertation
title:
Studies of phosphatidylinositol 3 kinase (PI3K) signaling pathway in
mammalian ovarian follicle activation and development
University dissertation from Umeå : Medicinsk kemi och biofysik
AUTHOR: Singareddy Rajareddy
KEYWORDS: oocyte; follicular development; P13K pathway; Medical Biochemistry
; medicinsk biokemi;
ABSTRACT: ignored and (iii) the overactivated follicles in p27-/- ovaries
are depleted in early adul... 阅读全帖
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