w****y
发帖数: 53 | 1 You could only have a sense of the serverity of the endogeneity problem.
Try to include additional variables in your model, and see how the results
will change. Another way is to assume some form of endogeneity, and then to
assess the robustness of your results when you vary the endogeneity level.
Per's paper is another useful exercise. |
|
f*******r
发帖数: 257 | 2 That's correct. There is no way to test endogeneity without a valid
instrument, unfortunately. Checking endogeneity based on graphs is at least
not main-stream.
endogenous |
|
i*******e
发帖数: 349 | 3 If I remember correctly, you need more instruments than possible endogenous
variables to test for endogeneity. |
|
f*******r
发帖数: 257 | 4 What I should have said is that you need instruments as least as many as
endogenous variables to do IV regression. With valid instruments, you can
do Hausman-Wu test to check whether IV regression is indeed needed. With
extra instruments, you can do over-identification test to check each
instrument's exogeneity. These in my opinion, it's not really a test of
endogeneity. But we don't have anything better... |
|
b*******n
发帖数: 605 | 5 Hi folks,
I am doing a CO-IP for an endogenous nuclear protein. I have tried several
protocols with acid wash but have not
been successful even once so far. Can anybody kindly share a protocol with
me if it works for you? Or can
anybody give suggestions for trouble shooting? Thank you! |
|
w********r
发帖数: 1431 | 6 如果是很难做的endogenous CO-IP,不妨先用DSP crosslink一下再做，
虽然很多人鄙视这种corsslink后做出来的CO-IP结果，但只要做好各种control，我觉
得其实也还可以。 |
|
h********n
发帖数: 4079 | 7 你老板是对的. 如果你做的是functional study, 一点要有endogenous. |
|
p******y
发帖数: 47 | 8 实验室当年发现了个蛋白，所有试验都是用overexpressioin做的，endogenous的蛋白
原来也做出来过，但是当年那个抗体不行了。老板又定了新的抗体，但是咋测都测不出
来。现在只有先IP再WB，但是即使用到2mg的蛋白做IP，还是测不到.TransctriptsS是
肯定有的，做过immunostaining 也饿凑凑活活能看见。就是WB不行。已经捣鼓了很长
时间了，很郁闷的说，请问大家有什么方法可以解决这个问题吗。谢谢大家。 |
|
H****N
发帖数: 997 | 9 I just replaced the endogenous orf with my orf. |
|
m*********n
发帖数: 158 | 10 endogenous protein pulldown,以前都是harvest cell lysis，然后antibody incubation overnight, next day add
beads for 1-2hours, and pulldown
听说把beads先和antibody cross link一下，可以提高pulldown efficiency;
所以准备把antibody-bead cross link,加到cell lysis,这样得话，大家一般incubate
在cell lysis里面多久呀？
谢谢 |
|
D******9
发帖数: 2665 | 11 请教一下， 我想做一个endogenous IP， 然后跑SDS-PAGE , coomassie blue染色后切
胶， 做MASS。
这个蛋白表达量不高， 需要用很多cells 然后IP， 估计得combine好几个IP后才能看
到带， 我用SDS-loading buffer 煮protein-antibody complex， 但是这样体积就太
大了， 没法load 到一个well， 有啥好办法解决这个问题？ 谢谢。 |
|
j***m
发帖数: 74 | 12 if I cannot find an instrument variable, is there anyway to test endogeneity
? |
|
s*****w
发帖数: 2065 | 13 how about thinking of the intuition
do you mean testing or solving?
endogeneity |
|
a**n
发帖数: 3801 | 14 why don't you just plot x vs. residuals?
endogeneity |
|
a**n
发帖数: 3801 | 15 not so formal..
if you can observe obvious trend, it's quite convincing that
endogeity exists..
however it's not so safe to claim no endogeneity if no trend
is detected by your eyes.. my 2 cents..
this paper maybe relevant
http://ideas.repec.org/p/hhs/umnees/0553.html |
|
h****i
发帖数: 1674 | 16 Is there any method to identify the endogeneity without extra instruments?
If we can easily find the instruments, we partly solve the problem already.
Still confusing |
|
d*****r
发帖数: 39446 | 17 【此篇文章是由自动发信系统所张贴】
endogenous 已经成为本俱乐部的正式成员, 特此通知. |
|
f**d
发帖数: 768 | 18 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
|
b*****d
发帖数: 61690 | 19 【 以下文字转载自 Biology 讨论区 】
发信人: Dua (Dua), 信区: Biology
标 题: 这人比陆俊更牛了，把自己名字PS到Nature paper上去了
发信站: BBS 未名空间站 (Fri Apr 19 00:16:31 2013, 美东)
新乡医学院药学院院长涉嫌多件学术造假
http://news.sciencenet.cn/htmlnews/2013/4/276959.shtm
上图为陈宙峰在《自然》杂志上发表的论文，下图为疑似被杨俊添加了自己名字的论文。
■本报记者 冯丽妃 唐凤
已发表的论文竟然被“偷”了！近期，美国圣路易斯华盛顿大学麻醉系教授陈宙峰居然
碰到这么一件不可思议的事儿。
一开始收到匿名检举人发来的邮件，陈宙峰还觉得是天方夜谭。然而，事实却让他不得
不信。
匿名检举邮件称，河南新乡医学院药学院现任院长杨俊把陈宙峰曾经发表在《自然》杂
志上的一篇文章“PS”上自己的名字后，附在其简历中，牟取利益。且有确切材料为证。
真有如此剽窃之人？震惊之余，出于好奇，陈宙峰在接下来的一个多月中，利用自己的
空暇时间对杨俊的信息作了些搜集。
在此过程... 阅读全帖 |
|
g********0
发帖数: 6201 | 20 【 以下文字转载自 Biology 讨论区 】
发信人: Dua (Dua), 信区: Biology
标 题: 这人比陆俊更牛了，把自己名字PS到Nature paper上去了
发信站: BBS 未名空间站 (Fri Apr 19 00:16:31 2013, 美东)
新乡医学院药学院院长涉嫌多件学术造假
http://news.sciencenet.cn/htmlnews/2013/4/276959.shtm
上图为陈宙峰在《自然》杂志上发表的论文，下图为疑似被杨俊添加了自己名字的论文。
■本报记者 冯丽妃 唐凤
已发表的论文竟然被“偷”了！近期，美国圣路易斯华盛顿大学麻醉系教授陈宙峰居然
碰到这么一件不可思议的事儿。
一开始收到匿名检举人发来的邮件，陈宙峰还觉得是天方夜谭。然而，事实却让他不得
不信。
匿名检举邮件称，河南新乡医学院药学院现任院长杨俊把陈宙峰曾经发表在《自然》杂
志上的一篇文章“PS”上自己的名字后，附在其简历中，牟取利益。且有确切材料为证。
真有如此剽窃之人？震惊之余，出于好奇，陈宙峰在接下来的一个多月中，利用自己的
空暇时间对杨俊的信息作了些搜集。
在此过程... 阅读全帖 |
|
D*a
发帖数: 6830 | 21 新乡医学院药学院院长涉嫌多件学术造假
http://news.sciencenet.cn/htmlnews/2013/4/276959.shtm
上图为陈宙峰在《自然》杂志上发表的论文，下图为疑似被杨俊添加了自己名字的论文。
■本报记者 冯丽妃 唐凤
已发表的论文竟然被“偷”了！近期，美国圣路易斯华盛顿大学麻醉系教授陈宙峰居然
碰到这么一件不可思议的事儿。
一开始收到匿名检举人发来的邮件，陈宙峰还觉得是天方夜谭。然而，事实却让他不得
不信。
匿名检举邮件称，河南新乡医学院药学院现任院长杨俊把陈宙峰曾经发表在《自然》杂
志上的一篇文章“PS”上自己的名字后，附在其简历中，牟取利益。且有确切材料为证。
真有如此剽窃之人？震惊之余，出于好奇，陈宙峰在接下来的一个多月中，利用自己的
空暇时间对杨俊的信息作了些搜集。
在此过程中，他发现，这位“杨博士”所涉嫌的学术不端行为并非仅此一件……
“真是想人之不敢想，
做前人未做之大事！”
“美国圣路易斯华盛顿大学教授陈宙峰有个有趣的发现。”3月4日，北京大学生命科学
院院长饶毅在发给《中国科学报》记者的一封邮件中称。
这封邮件同时记录了此前饶毅和陈宙峰... 阅读全帖 |
|
f*******r
发帖数: 257 | 22 This test is to do a Hausman test. It's not to test the endogeneity of the
instruments. It's not to test the endogeneity of the suspected endogenous
variables either. It's a test that given the exogeneity of instruments, do
you really need IV, or you are just fine to do OLS. There is no test to
test endogeneity of instruments. Overidentification test can kind of do
that, but in your case of exact identification, you cannot do that. |
|
p***y
发帖数: 18037 | 23 ☆─────────────────────────────────────☆
endogenous (源) 于 (Wed Sep 8 18:18:05 2010, 美东) 提到:
刚刚搬到纽约，现在在找房子，真的好烦啊。
Craigslist上骗子好多，世界日报上很少曼哈顿的房源。
因为还不确定呆多久，所以打算先找个sublet的。
现在的tenant要求我的ID copy, one month rent and one month deposit.
这样没有lease的sublet，想请教一下我应该怎么样防止上当受骗，可以问他要ID copy
吗？
听说纽约骗子挺多的，不知道还有没有别的什么注意事项，非常感谢！
☆─────────────────────────────────────☆
HudsonHank (芸芸众生,日久生情) 于 (Wed Sep 8 18:25:14 2010, 美东) 提到:
一防劫财， 二防劫色， 三防夺命。
其它的， 就没什么大不了的了。
☆─────────────────────────────────────☆
... 阅读全帖 |
|
l**********1
发帖数: 5204 | 24 Plus him:
Professor Qian (Q=Ch) received his B.A. in Astrophysics from Peking
University in China in 1982, and his Ph.D. in Biochemistry and Biophysics
from Washington University School of Medicine in St. Louis in 1989.
His current research areas are computaional systems biology at the cellular
level, molecular biophysics, and mathematical biology. These include large-
scale metabolic networks, protein interaction networks and signal
transduction modeling, protein thermodynamics and folding, and... 阅读全帖 |
|
b******e
发帖数: 88 | 25 我觉得你说的这个例子并不能说明recombinant protein不代表endogenous protein的
问题
而是一个类似于scientific misconduct的问题
因为这年头做结构的也要讲一个完整的故事（不然JBC都不收），问题是好不容易解出
来的结构并不一定都有那么多insight into the mechanism of xxx，或者说一时半会
儿看不出来。很多时候这就逼得结构生物学家赶鸭子上架，比如看到一个磷酸化位点就
敢说这个是调控的关键，也不顾（其实是没顾过来）跟biochem的数据是不是吻合。
至于recombinant是不是跟endogenous一样的问题，这当然不能完全证实或证伪。但我
想现在有不少关于表达recombinant multi-subunit complex的文章，这么大的复合体
（十几甚至几十个subunits，几个MegaDa的大小）都能被成功表达，活性或者功能都能
跟endogenous的一样，这已经很能说明recombinant蛋白的有效性了。 |
|
t****g
发帖数: 715 | 26 Simplified notations are introduced to show why you fail:
y_t= b * x_t + u_t (1)
Its lag:
y_{t-1}=b * x_{t-1} + u_{t-1}
Take a difference:
y_t-y_{t-1}=b*(x_t-x_{t-1})+(u_t-u_{t-1}) (2)
Endogeneity takes place in (1), i.e. x_t,u_t is correlated; now you consider
(2) and wonder whether endogeneity is gone, i.e. (x_t-x_{t-1}), and (u_t-u_
{t-1}) is uncorrelated? In general, this is not true.
Unless you make this strict assumption, you still suffer endogeneity. Could
you explain a |
|
s*******t
发帖数: 1743 | 27 thanks.
Wooldrige suggests a two-step procedure to test the endogeneity of a single
explanatory variable:
step1: estimate the reduced form for Y_2 (suspected to be endogenous) by reg
ressing it on ALL exogenous variables,including intrumental variables and th
ose in the structral equation, and save the residuals
step2: add the residuals to your structural equation and test its significan
ce. If it is sig, we conclude that Y_2 is endogenous.
consider
u_
Could |
|
d******e
发帖数: 7844 | 28 In a statistical model, a parameter or variable is said to be endogenous
when there is a correlation between the parameter or variable and the error
term. Endogeneity can arise as a result of measurement error, autoregression
with autocorrelated errors, simultaneity and omitted variables.
endogenity是学经济的人造出来的词？ |
|
d******e
发帖数: 7844 | 29 In a statistical model, a parameter or variable is said to be endogenous
when there is a correlation between the parameter or variable and the error
term. Endogeneity can arise as a result of measurement error, autoregression
with autocorrelated errors, simultaneity and omitted variables.
endogenity是学经济的人造出来的词？ |
|
|
l**********1
发帖数: 5204 | 31 Lastly,
Yes,申请General Medical Sciences which is 最多资助比率的
one sample:
Project Detail
The Role Of Host Endogenous Small Rnas In Innate Immunity
Project Number: 5R01GM093008-02 Source: National Institutes of
Health
Principal Investigator: Hailing Jin Organization: University Of
California Riverside
Project Duration: 07/01/2011 - 06/30/2012 Fiscal Year: 2011
Recovery Act: No Award: $281,250
Application Type: Non-competing Continuation
Funding Institution: ... 阅读全帖 |
|
w*****t
发帖数: 179 | 32 您好，不知道我的背景能否审到这片文章。我曾经审过一些小杂志，希望能够对我争取
这次机会有所帮助。详细的CV和研究背景如下。非常感谢您的考虑。我的工作邮箱：
z****[email protected]
好像CV在这里粘贴很难看，不好意思。前一段时我的CV，后一段我的较详细的研究经历
。如有需要我更详细的背景情况，请跟我发信。
1
CURRICULUM VITAE
Full Name: Zhimin Wang
Address: Allegheny-Singer Research Institute, West Penn Allegheny, Health
System, South Tower, 320 East North Avenue, Pittsburgh, PA 15212-4772
Phone: 412-359-6153 (Office)
412-737-3777 (Cell)
E-mail: z****[email protected] w************[email protected]
EDUCATION
September, 2001- May, 2005 Ph.D., Molecular M... 阅读全帖 |
|
w*****t
发帖数: 179 | 33 hello, thank you for providing the information. Please find my CV below and
my research experience. I have been revewing for over 10 journals for 14
times. My majour of PH.D is microbiology from Wuhan university. I have
published several papers concering virology and bacterial infection. So far
my work also foucse on salivary gland biofilm formation under LL37 and K4s4
treatment. If you need further information, please let me know. My email
address: z****[email protected]
Thank you for your time and c... 阅读全帖 |
|
S****e
发帖数: 10596 | 34 我是最不喜欢浪费粮食的人
看到很多人讨论扔掉鸡蛋黄我实在觉得很不好
我上网随便搜了点文章
一篇中文一篇英文
在大众科学宣传中，一直把胆固醇作为人类健康的敌人。因此，鸡蛋也被认为是一
个罪魁祸首，因为它的蛋黄里含有大量胆固醇。但是，在你扔掉蛋黄之前，有几件
事情应该首先弄清楚。
1、胆固醇对于身体功能是必不可少的
细胞膜的构建，维护和正常运作需要胆固醇。没有胆固醇，你的细胞将难以发
挥作用。胆固醇也帮助生产有利于消化脂肪的胆汁。胆汁有助于代谢脂溶性维生素
A，D，E和K。而所有这些脂溶性维生素和胆固醇一起存在于蛋黄中。
2、身体产生的胆固醇比吃的多
血液中的胆固醇主要来自肝脏和饮食。身体产生的胆固醇数量与摄入的多少有
关系。当吃低胆固醇饮食时，身体就会自己产生更多的胆固醇，这也是为什么仅仅
依靠低胆固醇饮食不能减少血液中整体胆固醇水平的原因所在。
3、高胆固醇本身并不会导致心脏病
高胆固醇有助于抵御感染。实际上，血液中胆固醇高的人和较低的人患心脏病
的概率是一样的。问题的关键在于身体中好胆固醇（高密度脂蛋白）和坏胆固醇（
低密度脂蛋白）之间... 阅读全帖 |
|
a*******s
发帖数: 117 | 35 自然发生的跨远距离物种基因转移举例：
病毒基因转移到哺乳动物，包括人类
PNAS(2012)
Ancestral capture of syncytin-Car1, a fusogenic endogenous retroviral
envelope gene involved in placentation and conserved in Carnivora
ABSTRACT
Syncytins are envelope protein genes of retroviral origin that have been
captured for a function in placentation. Two such genes have already
been identified in simians, two distinct, unrelated genes have been
identified in Muridae, and a fifth gene has been identified in the
rabbit. Here, we searched for s... 阅读全帖 |
|
d********f
发帖数: 43471 | 36 【 以下文字转载自 Biology 讨论区 】
发信人: nyimmune (加油), 信区: Biology
标 题: 我被怀疑造假的经历
发信站: BBS 未名空间站 (Tue Aug 2 21:56:42 2016, 美东)
我自己的经历，被怀疑造假也没什么，不需要什么第三方权威人士验证，把怀疑最厉害
的人说服了就可以的。
前几年离开原来实验室做公司去了，留下一篇基本写好的manuscript，老板让接手的烙
印博后小兄弟做个co first author补点数据准备投个大文章。没想到接手的小兄弟不
好好补数据却去检验我做好的部分，过了些日子老板抱怨我的数据不可重复，因为跟老
板本来关系特别好像朋友一样，如果造假就像坑朋友的饭碗当然是特别严重的。所以特
别安排了一个礼拜的时间回到原来的城市，看看小兄弟究竟有什么实验操作问题，全程
烙印动手我就看看加动嘴皮子。
其中一个实验是endogenous co-IP，烙印说是对照的抗体也有pull-down和阳性的差不
多，而我的数据里面对照是干净的。抗体beads结合完了以后需要清洗，我看烙印把离
心机设定到8000rpm3min，当... 阅读全帖 |
|
m*****n
发帖数: 3644 | 37 没用过。但是你说的高级程序也远不如人bid,因为无法哄抬物价吓制其他人从而拿到更
低报价，而且也可以保证拿到，而sniper只能保证不搞出自己的心理价位，差太远了。
其次，专业点说，sniper的报价使quasi endogenous,而自己的是完全endogenous，自
然可以拿到更低价格。
当然，时间上值不值得是另一回事。
bid |
|
M****e
发帖数: 70 | 38 is there IP assay available? i think it is necessary if you
can fractionate the protein by columns (many biochemcal stuffs
that i am not familiar, which i read from purification of
p300 transcription factor, i think people in this area knows
a lot). then do the assay to see which fraction has the
activity (is this specific reaction?) thus you know the effective
size.
is this endogenous or expressed protein? guess it is
endogenous, given the big size. try IP with the antibody and
do the assay. or |
|
a***e
发帖数: 1010 | 39 not really.
endogenous siRNAs target endogenous genes.
miRNA can also silence virus genes. |
|
C*******e
发帖数: 4348 | 40 我没有说细菌X来源的endogenous plasmid直接测序测不出
我说E.coli的那个测不出
细菌来源的我前天才提的，不多，做PCR可以，测序不够，所以还没有测序
我同意你的说法E.coli的那个plasmid多半有些什么问题
但是我用细菌X的endogenous plasmid做PCR出来的结果是一样的 |
|
S*****s
发帖数: 287 | 41 谢谢楼上各位。我正在看这方面的文章，特别是七楼 jcb 老兄列出来的那篇，越看越
觉得不错。我做的蛋白目前没有 endogenous cell line，而我研究的方向也包括 mRNA
intron splicing。现在我都是用引入了 CMV promoter 的 BAC DNA 做 transfection
。看看这些文章我倒是想买点这个 ZFN 然后把 HEK293T 细胞的基因组改一改，改造出
一个可以 endogenous cell line 来。明天和 sigma 的 rep 继续谈谈。 |
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j********r
发帖数: 156 | 42 最近一直在尝试overexpress a gene in primary epithelial cells, but always
failed to get decent level of expression。望版上诸位帮看一下。
我用的是一个类似与pBabe的retroviral vector, the gene was tagged with GFP or
Flag on the c-terminal. The target cells were infected twice and then
subjected to antibiotic selection (blasticidin in this case). 用anti-
Flag做western, 勉强能看到带，如果用the ab against itself, 可以看到exogenous和
endogeneous的差不多 （the endogenous level is already very low, which is
also the reason that we hope to increase it to ... 阅读全帖 |
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y*********u
发帖数: 183 | 43 We replaced the part of exon1,and exon2, do you think the endogenous pA
would work in this case? And could you post a link regarding the case of
utilizing the endogenous pA ?
it |
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l******n
发帖数: 298 | 44 谢楼上各位。蛋白是血管平滑肌特异性akt.SM22-HA-AKT,加了一个tag的话是90kd,
endogenous是50多，两个能区分。
分离蛋白是匀浆3次，用的是millipore的kit,270块钱能做13毫升的样品。kit里面有
peotease, phosphatase inhibitor mix。
上western, 用cell signalling的抗体，磷酸化和未磷酸化的AKT条带都没有，
endogenous 磷酸化和未磷酸化的条带都有。
因此对他的这个老鼠表示怀疑。不过他给我几个老鼠做genotyping的话还是100%准确，
确实有transgene.今天他们说这个promoter表达很低，我就不知道有多低 |
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h******y
发帖数: 1374 | 45 The Nobel Prize in Physiology or Medicine 2011
shall be divided, with one half jointly to
Bruce A. Beutler and Jules A. Hoffmann
for their discoveries concerning the activation of innate immunity
and the other half to
Ralph M. Steinman
for his discovery of the dendritic cell and its role in adaptive immunity
Summary
This year's Nobel Laureates have revolutionized our understanding of the
immune system by discovering key principles for its activation.
Scientists have long been searching for the g... 阅读全帖 |
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u**********d
发帖数: 573 | 46 如果少量过表达你的TF（比如挑一个克隆，exogenous与endogenous量差不多）对你已
经得到的那些主要结果没影响的话，还是很有用的。可以带两个tag，比如FLAG和HA，
这样的结果就更可靠了。但是变态的reviewer可能还是会要你endogenous的结果，TNND！ |
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a*****2
发帖数: 175 | 47 paraffin- or cryo- 都做过,cryo的信号好一些,最好切完一个星期以内作.
endogenous的biotin有kit可以inhibit,好像是vectorlab.不过,我用的是DIG labell的
probe.
降低背景,还需要作acetylation.这个步骤也可以替换成0.2NHCL处理10min.
最后最后,target gene的表达量是多少,如果太低,背景都会很高.
endogenous |
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l**********t
发帖数: 5754 | 48
Factors "exogenous" to a theory are factors that are taken as given, not
generated/derived within the theory (endogenous factors).
The (changes) of habitats/environments within which natural selection occurs
are exogenous to the evolution theory -- taken as given/assumed (based on
after-the-fact observation), not endogenous (dervied/modeled/predicted by
the theory). |
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w***r
发帖数: 709 | 49 挺牛但是长期效果，尤其是复发情况有待观察（个人不太看好，为啥肿瘤一定要有免疫
原性？？）
谁知道相关文章推荐一下。
Safety and Activity of Anti–PD-L1 Antibody in Patients with Advanced Cancer
(NEJM 2012)
Background: A major implication of the clinical activity of immune
checkpoint blockade is that significant endogenous immune responses to tumor
antigens are generated, and these responses may be harnessed
therapeutically to mediate clinical tumor regression on checkpoint
inhibition. An emerging concept in cancer immunology is that inhibitory
ligands su... 阅读全帖 |
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w***r
发帖数: 709 | 50 "Super p53" mice exhibit enhanced DNA damage response, are tumor resistant
and
age normally.
García-Cao I(1), García-Cao M, Martín-Caballero J, Criado LM, Klatt P,
Flores JM,
Weill JC, Blasco MA, Serrano M.
Author information:
(1)Spanish National Center of Biotechnology, Department of Immunology and
Oncology, Campus de Cantoblanco, Madrid E-28049, Spain.
The tumor suppressor p53 is critical in preventing cancer due to its ability
to
trigger proliferation arrest and cell death upon the occurrence... 阅读全帖 |
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