x***4
发帖数: 1815 | 1 bf3里面我觉得俄国三人突击小队的那两个关卡设计的很好。节奏非常快，室内光影精
彩，还可以左右穿插flank敌人，可惜都太短了。如果设计得长一点，可以３人co-op就
完美了。
mw系列我觉得印象最深刻的就是mw１的刺杀关卡了。 |
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t******g
发帖数: 17520 | 2 BB和MK， 地图有choke point, 把mob引出，卡住以后事半功倍， 漫图乱跑
容易被人flank， 皮厚被人围没事， 菜鸟皮薄的WD的我被人围住情何以堪啊
人多，normal 没事， 要往后打得话， 2个人还是要注意一下
没有抱怨， 提醒而已 |
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i*******8
发帖数: 7955 | 3 beta上只有拆弹埋弹，不过据说两人组队(fire team)是核心
After a few hours, I had several classes unlocked and was familiar with each
, bringing out the true power of the fire team mechanic. There are tons of
different combinations for different situations – a particularly effective
combo involved the demolitions class, a walking tank with extra body armor
and an automatic shotgun. The demolitions class has a visor that can be
lowered for even greater defense, making for a great mobile shield the other
player can hid... 阅读全帖 |
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c*******i
发帖数: 142 | 4 我开个头，
比如metro这张图，b点还没拿稳就急着往前冲，防线都不用攻就成筛子了。
一帮2b堆在locker room那条走廊，一个雷死一片，一梭子打死一串。就不能分散点么？
防守只防一边，敌人flank一次一个准。
有一帮noob就是爱跟大部队，5，6个人冲上去强奸一个人很爽是吧，没一会几个人就被
一个人奸了。
最傻逼的其实不是以上这些，而是站在flag以外1mm距离晃悠，就是不capture flag。 |
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K***j
发帖数: 316 | 5 全力搞卫星，把前两三个月撑过去就是胜利，有一个两个国家退出council无所谓，只
要保证每个大陆都还有国家代表就可以了。多用arc thrower俘获敌人，然后
interrogate可以得research credit，而且敌人的plasma武器也很好用。个人觉得
laser武器性价比不好，不如从常规武器一步到位plasma。
士兵方面sniper非常逆天，刚开始的时候很烂，但一定要升级squad sight，这样可以
狙击在队友视野里的敌人，加上后来的damn good ground（居高临下射击damage bonus
)和in the zone(射击被flank的敌人不耗行动点）之后就是杀人机器了，经常可以一个
sniper横扫全场（当然需要assault上前侦查，最后穿上ghost armor隐身）。而且
Sniper射击成功概率被设定成和距离成反比，敌人离得越远打得越准。 |
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L**********s
发帖数: 12988 | 7 18. Extended Side Angle Pose: Utthita Parsvakonasana
(oo-TEE-tah parsh-vah-cone-AHS-anna)
utthita = extended
parsva = side, flank
kona = angle
Benefits:
- Strengthens your thighs, hips, knees, and ankles.
- Stretches your groin, back, spine, waist, ankles, lungs (intercostals) and
shoulders.
- Massages and stimulates your abdominal organs.
- Increases endurance and stamina.
Cautions:
- High or low blood pressure
- Knee injuries
- Headache
- Insomia
- Neck Problems – Keep your gaze in the same di... 阅读全帖 |
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s*********t
发帖数: 1663 | 8 理想情况当然是能有兵去flank了
问题是星际2 zealot的速度提升和星际1的速度根本不是一个东西
因此我没有看到一个更好的方案，针对昨天inca那第一盘。
另外神族很难接受分兵，理由我前面帖子里讲了，没有DPS
别说3个stalker从天而降
就是8个也不会对人族造成什么伤害
神族唯一能让你认为出彩的估计是forcefield了，问题对人族效果很差，射程问题，奏
效的必然是人族重大失误。 我对sentry的建议就是打人族根本别出，或者升级幻象(有
没有用我不清楚)，或者出一个放防护罩，forcefield就算了吧。sentry还浪费gas，前
期gas太重要了 |
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s**a
发帖数: 8648 | 9 图书馆里面霍乱的中文版都被劫走了。英文版（edith grossman翻译本）pubis出现两
次，第一次是阿里撒画画写字在他情人身上，结果要了她的命：
In a sudden inspiration, Florentino Ariza opened a can of red paint that was
within reach of the bunk, wet his index finger, and painted the pubis of
the beautiful pigeon fancier with an arrow of blood pointing south, and on
her belly the words: This pussy is mine.That same night, Olimpia Zuleta
undressed in front of her husband, having forgotten what was scrawled there,
and he did not say a word, his breathing did not ... 阅读全帖 |
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r****e
发帖数: 3373 | 10 LeisureTime版 - sasa，请教你一个无耻的问题
iamwhatieat
2011-01-27 15:29:13
来自: 24.159.
1
霍乱时期的爱情我90年读的。分明记得那版紫色书皮，最后阿里萨终于得手时，费尔米
纳已经老朽，＂耻骨上的毛＂都没多少了。前几天网上闲翻，版本显然更新，但译笔粗
糙，更可恶的是，根本就没有关于耻骨的描写。。不禁有一点怀疑，难道自己记错了？
你是马尔克斯专家，所以不揣无耻，把这个关于耻骨的问题写出来，请你解惑。
izel
2011-01-27 15:31:35
来自: 129.59.
2
无耻！下流！
wh
2011-01-27 15:34:22
来自: 67.86.
3
乐子！你让我们版损失2000伪币！星版搞十大星星活动，每个得奖id有200，版面又有
2000伪币。臭乐子！
izel
2011-01-27 15:35:07
来自: 129.59.
4
庸俗！
wh
2011-01-27 15:38:53
来自: 67.86.
5
包子是王道！这是你说的！
lhr
2011-01-27 15:43:04
来自: 137.71.
6
发包... 阅读全帖 |
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b*s
发帖数: 82482 | 11 赞！
多谢详图。这样的炮配制有些古怪，但是也算是能够work吧，估计也是由于不好用，以
后就淘汰了，跟英国的defiant fighter一样，最终被淘汰了：
英国的defiant fighter只有后炮塔对后方开炮，前方没有武器！
不过说起来，只有一边的炮火能够使用也不是什么新鲜事情，古时候的船舷装炮的军舰
都是只有一边对敌的，除非两边受敌，双边齐射，叫做double broadside...
北洋的队形确实是有问题：
Admiral Itoh ordered the Japanese flying squadron to strike at the weak
Chinese flank. On observing the movement, Admiral Ding realized that his
formation prevented the Chinese battleships in the center from firing
because their smaller companions were between them and their opponents a... 阅读全帖 |
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i*********t
发帖数: 5873 | 12 references。。
发信人: wh (wh), 信区: WebRadio
标 题: Re: 来学习波哥的讲话
发信站: BBS 未名空间站 (Wed Oct 7 14:07:37 2009, 美东)
他说的原文是这篇，很长：
发信人: iamwhatieat (我吃故我在), 信区: Reader
标 题: Fascinating Fascism
发信站: BBS 未名空间站 (Thu Oct 1 02:19:18 2009, 美东)
Fascinating Fascism
by Susan Sontag
New York Review of Books
February 6, 1975
republished in:
Under the Sign of Saturn
(New York, 1980), pp. 73-105.
First Exhibit. Here is a book of 126 splendid color photographs by Leni
Riefenstahl, certainly the most ravishing book of phot... 阅读全帖 |
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wh
发帖数: 141625 | 13 昨天刚看了几眼up in the air，今天就看到george clooney的威尼斯婚礼新闻，说53
岁的他是the film world's most eligible bachelor，这场婚礼是one of the most
eagerly anticipated events of the showbusiness year——有这么夸张吗？什么叫
most eligible bachelor？很有钱还是很花……他演技好吗？我咋一直觉得很虚假的样
子……
他的human rights lawyer新娘很潮啊，不输满座——or满船的济济嘉宾。看图吧：
Film star George Clooney marries in Venice
27 September 2014, BBC
Hollywood star George Clooney has married human rights lawyer Amal Alamuddin
in Venice, in one of the most eagerly anticipated events of the
showbusin... 阅读全帖 |
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a*o
发帖数: 25262 | 14 http://www.newyorker.com/magazine/2014/10/13/cooka%C2%80%C2%99s
The Kitchen Network
America’s underground Chinese restaurant workers.
By Lauren Hilgers
Chinatown employment agencies can get immigrants kitchen jobs in a few
hours. Credit Photograph by Annie Ling
In a strip mall on a rural stretch of Maryland’s Indian Head Highway, a
gaudy red façade shaped like a pagoda distinguishes a Chinese
restaurant from a line of bland storefronts: a nail salon, a liquor store,
and a laundromat. On ... 阅读全帖 |
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S******s
发帖数: 179 | 15 http://answers.yahoo.com/question/index?qid=20071121231328AAplpoF
Slowly a tank creeps out of the mist with soldiers in gas masks on either
flank, followed by a convoy of more tanks and soldiers with flame throwers.
In their wake the mist is thinning and soliders are burning one monster
after another while trucks pass by carrying survivors. |
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a*******u
发帖数: 6324 | 16 我觉得还是得从flank开始练，所谓的外练筋骨皮，内练一口气。
那筋骨皮嘛，自然是牛腩这些多筋有嚼头的地方了。
不行，饿了，我回家得去煎一块牛排去。 |
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a*******u
发帖数: 6324 | 17 对啊，就是以腰部为支点，那个拧措劲儿。
一甩，高音就出来了。
所以我还是觉得练好flank才行，腰窝嘛。 |
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l***a
发帖数: 12410 | 18 nonono
牛腱是shank，牛腩是flank |
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s*****e
发帖数: 21415 | 19 ☆─────────────────────────────────────☆
KittyKat (兮若妞儿她娘) 于 (Wed Jun 22 16:56:54 2011, 美东) 提到:
发信人: KittyKat (兮若妞儿她娘), 信区: Food
标 题: 兮若娘之【白萝卜炖排骨】
发信站: BBS 未名空间站 (Tue Jun 21 20:17:54 2011, 美东)
好久没上菜啦~这次上个汤吧~~
排骨飞水加姜片料酒去血沫去腥味 冷水冲洗
锅稍微热下 放入排骨+姜片稍微炒一炒 然后放点花椒八角桂皮 加入足量水
转移至砂锅内 加入陈皮 大火烧开转小火 慢炖3-4小时
白萝卜去皮切滚刀块 开水烫一下去除苦涩味 凉水冲洗备用
待排骨炖好之后 放入白萝卜 大火烧开转小火炖半小时
然后就可以出锅了~盛到碗里 加葱花 盐 鸡精~开喝~~~@——@
☆─────────────────────────────────────☆
apolitical (无期徒刑) 于 (Wed Jun 22 16:57:15 2011, 美东) 提到:
我赌200个回帖。
☆───... 阅读全帖 |
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j******0
发帖数: 1043 | 20 battleship
大烂片
看了N遍
最令人尊敬的就是那些战舰上的老兵
老舰长就两句台词
All engines ahead flank!
Let's drop some lead on those motherfucker! |
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z*********i
发帖数: 1059 | 21 The Battle of Shiting was fought between the contending states of Cao Wei
and Eastern Wu in 228 during the Three Kingdoms period of Chinese history.
The battle concluded with victory of Wu over Wei.
[edit] The battle
Sun Quan ordered Zhou Fang to lay a trap for Cao Xiu, the commander-in-chief
of Wei, by pretending to defect. Cao Xiu was deceived into leading his
troops straight into Wan. Then, Sun Quan appointed Lu Xun as Grand Chief
Commander, commanding him to lead an attack against Cao Xiu. O... 阅读全帖 |
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l****j
发帖数: 114 | 22 在我工作的地方，任何育龄的female进来提到肚子疼，不管是stomach pain还是flank
pain还是pelvic pain，不管号称有没有sexual active在不在吃避孕药，一律先留尿查
怀孕了没有。 |
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B***i
发帖数: 1208 | 23 【 以下文字转载自 Joke 讨论区 】
发信人: bread22 (bread22), 信区: Joke
标 题: 贝总真是个充满诗意的行为艺术家 (转载)
发信站: BBS 未名空间站 (Sat May 18 02:19:14 2013, 美东)
发信人: ARod (Light be with you.), 信区: Soccer
标 题: 贝总真是个充满诗意的行为艺术家
发信站: BBS 未名空间站 (Sat May 18 00:44:24 2013, 美东)
发信人: sealy (天佑中华), 信区: Juventus
标 题: [无关]贝秃性爱派对中曾让女模化妆成。。。
发信站: 水木社区 (Sat May 18 11:03:19 2013), 站内
奥黑和小罗。。。
Dancer says showgirls dressed as Obama, nuns at Berlusconi party
By Sara Rossi
MILAN | Fri May 17, 2013 11:55am EDT
(Reuters) - The nightclub danc... 阅读全帖 |
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f****b
发帖数: 2410 | 24 http://www.theguardian.com/politics/2013/nov/27/blair-murdoch-g
Tony Blair: I only come in peace.
Rupert Murdoch: That's not what I'm hearing, sonny.
Blair: What are you talkin' about?
Murdoch: According to my sources …
Blair: Not phone-hackers, I hope.
Murdoch: Don't get clever with me, sport.
Blair: Now guys ... I mean, Rupert ... Let's just calm down a bit, shall we?
Let me categorically state this: I never made wild and beautiful love to
that woman while the coyotes howled outside under the ... 阅读全帖 |
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b*****2
发帖数: 11103 | 25 【 以下文字转载自 Soccer 讨论区 】
发信人: ARod (Light be with you.), 信区: Soccer
标 题: 贝总真是个充满诗意的行为艺术家
发信站: BBS 未名空间站 (Sat May 18 00:44:24 2013, 美东)
发信人: sealy (天佑中华), 信区: Juventus
标 题: [无关]贝秃性爱派对中曾让女模化妆成。。。
发信站: 水木社区 (Sat May 18 11:03:19 2013), 站内
奥黑和小罗。。。
Dancer says showgirls dressed as Obama, nuns at Berlusconi party
By Sara Rossi
MILAN | Fri May 17, 2013 11:55am EDT
(Reuters) - The nightclub dancer at the center of former Italian Prime
Minister Silvio Berlusconi's "bunga bunga" sex trial told a cou... 阅读全帖 |
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K***a
发帖数: 5176 | 27 记得蛮蛮妈说蛮蛮就爱吃raw，昨天我做土豆炖牛腩，牛肉很好很新鲜，但因为有筋，
很不好切，我就跟牛肉在厨房这么撕扯着。。后来突然发现酥饼不知不觉的已经来了，
把地上掉了肉渣都给吃了，然后守在那儿了！！
我又试着手喂了他一点点，他也很有兴趣的吃了，让我大吃一惊！！这娃怎么回事，这
是在告诉我他要吃raw么？（好的牛肉确实很香啊。。。。）我想也可以慢慢给他一点
点吃，想问下牛肉喂猫猫好么？是不是鸡肉是最好的？我买的是beef flank，是我自己
吃的牛腩，他们吃什么部位最好？哎哟一大堆问题~~~先谢谢啦。我想一周喂一次什么
的吧。。先慢慢来吧。。蛮蛮是每天都吃么？MM还给他加什么supplements么？ |
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c*******u
发帖数: 800 | 28 几岁了？做了吗？上次持续了多久长回来的？痒不痒？两边都掉毛吗？
先排除allergy或者寄生虫，然后怀疑recurrent flank alopecia |
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d**********6
发帖数: 242 | 29
2岁，女太监。持续快一周了。发生在两侧，不痒，没症状。但是皮肤干燥，头皮削增
加。
不是allergy，吃的没变化，环境没变化，而且她allergy一般表现在大便。
recurrent flank alopecia是反复性掉毛？是病吗？感觉挺像的。 |
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e*****n
发帖数: 3129 | 30 http://foxyurl.com/rua
To be fair, Michael Jackson didn't know it was his last day. Jesus did.
Michael Jackson spent the last night of his life performing, in rehearsals
at the Staples Center, running through a slate of songs for his upcoming 50-
concert London, flanked by friends and colleagues.
"He was just glowing, and you could see it, that he was finally seeing
it all come together."
MJ left rehearsals right before midnight and headed off to attend yet more
meetings. He died 12 hours l |
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t****r
发帖数: 592 | 31 yes I agree that she must feel good so she forwarded articles. I has said I
appreciated her works. But flankly to say, just now I felt curious and
searched her articles on SD board, I felt a little dissaponted... I hope she
enjoy more articles here... :-) |
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C********g
发帖数: 9656 | 32 http://my.cnd.org/modules/wfsection/article.php?articleid=27005
·廖 康·
英国19世纪浪漫主义诗人济慈(John Keats，1795－1821)看着一个希腊石雕古瓮上的形
像情不自禁大发思古之幽情：你这秀丽的姑娘，雕在古瓮上。在静默之中保持完美，不
曾受到人世纷争的搅扰和岁月无情的摧残。穿越时空，形体依旧，仿佛在向我讲述你花
样年华的故事，比任何诗歌都更加美丽动听。环绕着你这美丽的姑娘，曾有多少神奇的
传说？在希腊昙邳或阿卡狄的溪谷里，有多少神和人曾经追求你的青睐？他们曾经怎样
疯狂地追求？吹着什么笛子？打着什么鼓？陶醉在什么样的狂喜之中？那是些什么神和
人呀？竟然都不值得你以身相许？
凝视着古瓮上欲吻未吻的一对青年男女，济慈感慨道：听见的乐声再美妙，也比不上没
听见的乐声。雕刻在树下的吹笛人，你尽情吹吧，我虽然听不见，但你无声的乐曲吹入
我的灵魂，永无终止。如同雕刻的树木一样，树叶永不凋零。大胆的恋人也是一样，你
已经接近这位美女，但却永远、永远也吻不上。可是不要悲哀，这样，她将永远美丽，
你将永远热恋如初！
由... 阅读全帖 |
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S*********e
发帖数: 3006 | 33 <题外话>
1) 约翰*济慈生于1795年。幼丧父母，由祖母抚养长大。他的弟弟汤姆1818年死于肺结核。济慈在照顾汤姆的过程中不幸被感染，两年后去世。死时年仅25岁。
2) 我第一次知道济慈是在进大学不久的一次诗歌社团活动。主持活动的师兄刚介绍完济慈的生平，下面立刻响起一个女生的轻呼：(这么年轻就死去)太可惜了。而我的第一反应是：济慈的祖母会非常、非常伤心吧。。。不知为什么，这个镜头一直在我的记忆里清晰地存在，而大学时代其他很多更重要的事情却慢慢淡去。
3) 济慈最著名的作品当然是<<夜莺颂>>。他本人学医。所有诗作几乎都是在去世前两年创作。下面翻译的<<希腊古瓶>>，直译的话是<<对一个希腊瓶子的颂歌>>，是济慈数首颂歌之一。我本人也是不久前才偶尔读到、认真欣赏。这是对一个希腊古瓶的怀古之作。古瓶上应该画着树叶、人物、村庄等等。
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
[希腊古瓶]
原作：约翰*济慈
你这安静的、未被玷污的新娘！
你没有亲生父母 --
养父是永恒之沉默，而养母
是缓慢而坚定流逝的时光。
于是你脸上画的树木，
真实地低声地
把甜蜜委婉充满... 阅读全帖 |
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p****s
发帖数: 32405 | 34 Cruyff’s cardiac problems forced him to give up cigarettes but, sucking furiously on lollipops, he oversaw the emergence of a team that encapsulated his vision. Bar?a won the league in 1990–91 and went on to win it in each of the next three seasons as well. Even more importantly, in 1992 the Dream Team, as it became known, became the first Barcelona side to win the European Cup. Stoichkov happily played wide in that line-up, usually on the left but occasionally on the right; Laudrup by then was ... 阅读全帖 |
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D*****r
发帖数: 6791 | 35 A SPEECH OF Mr. JOHN MILTON
For the Liberty of VNLICENC'D PRINTING,
To the PARLAMENT of ENGLAND.
______________________________________________________________
This is true Liberty when free born men
Having to advise the public may speak free,
Which he who can, and will, deserv's high praise,
Who neither can nor will, may hold his peace;
What can be juster in a State then this?
... 阅读全帖 |
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vi
发帖数: 309 | 36
西游记里面的那个如来佛的原型是哪个佛？
书上说Boddha是已经脱离轮回；而Bodhisattva是虽然可以脱离轮回，
但是主动留下帮助世人，他们还有最后一个轮回没有经历。所以看
起来，至少从字面上这两者是有区别的。
寺庙中菩萨都是站在佛像两侧护卫flank的。地位尊卑很明显。 |
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y**********6
发帖数: 624 | 37 beef flank或beef brisket |
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w********1
发帖数: 3492 | 40 Sun, 15 Jul 2012 16:15:37 PDT
The New York Times has added to the concerto of reports hinting at the
arrival of an iPad with a 7.85" screen this fall. Two weeks ago, Bloomberg
offered a similar report, also citing anonymous sources. iMore reported
something similar back in May.
Mockup of 7.85-inch iPad next to an iPad 2 (courtesy of CiccareseDesign)
The company is developing a new tablet with a 7.85-inch screen that is
likely to sell for significantly less than the latest $499 iPad, with
its... 阅读全帖 |
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s*****t
发帖数: 1994 | 41 Apollo 17: Last on the Moon
Credit: Apollo 17, NASA (Image scanned by Kipp Teague)
Explanation: In December of 1972, Apollo 17 astronauts Eugene Cernan and Harrison
Schmitt spent about 75 hours on the Moon, in the Taurus-Littrow valley, while colleague
Ronald Evans orbited overhead. Near the beginning of their third and final excursion across
the lunar surface, Schmitt took this picture of Cernan flanked by an American flag and their
lunar rover's umbrella-shaped high-gain antenna. T |
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n*******e
发帖数: 27 | 42 use NEBuffer2+BSA, digest enough amount of PCR product O/N. i do not know
if you add some nucleotides for buffer purpose. if it is for mutagenesis,
or expression of certain gene, maybe difficult. however, check with BioLab
for Hind3, even 12bp sequence (6bp as buffer flanking) could not be completely
digested O/N. for Xho, it is better, about 75%. so the 1st thing you should
do is to check your design, order new primers if necessary. they are so
cheap nowadays. actually, since it is only 150bp. |
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y****e
发帖数: 71 | 43 If contamination goes 99.9% in all clone, that means you get a supercoiled
circular DNA as your contaminant. It transforms at a much higher efficiency.
First thing you might do is change plasmid.
I talked it to my boss, he had an interesting theory. If your insert confers
toxicity to E.coli, he said there might be some wierd recombination event that
cut the insert out of the plasmid, along with flanking reagions (which might
contain the MCS, that is why you cannot cut it again with your REs). |
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f**********s
发帖数: 1415 | 44 裸鼠flank接种人源肿瘤细胞，长到150-200 mm左右给药，一个月后发现对照肿瘤也只
长了一倍左右，是不是不太正常? 如果不正常，可能是什么原因呢？ |
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X***n
发帖数: 366 | 45 how about using pKD46 to flank the gene you want to knock out with FRT,then
insert FLP gene downstream the promoter that activate at the specific time
point?
I don't know details :) |
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X***n
发帖数: 366 | 46 I cloned 400-500 bp miniarms cloned into ptdTomato-C1, and then linearized
it with EcoRI, so that the backbone was flanked by miniarms on both ends. I
electroporated the linearized plasmid into the induced Red recombinases in
SW102 carrying my BAC. All the clones turns to be empty. I succeed in the
other two using pKD46 system but failed in this one. And now I also fail
using SW102 system. What's the most possible reason? Thanks in advance. |
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g*********5
发帖数: 2533 | 47 I think mutation is the easiest way to do. just with quikchange kit, about
200 bucks.
because your pcr fragment is smaller than 30 bp, so you could design primer
with this sequence and flanked with vector sequence, 15 bp each, then use
this 60 bp primer pair do insert mutation, just one PCR and transform.
good luck |
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b******r
发帖数: 111 | 48 I need PCR 5-flanking of mouse APOE genomic DNA(9kb upstrean of exon 1),and
human APOE3,4 genomic DNA(4kb). I have tested 10 primer pairs and their
combinations.But till now,can not get the target PCR product.Maximum is non-
specific 2-4 kb Anyone knows any vectors and their vendors contain the
mouse and human APOE full length genomic DNA ? |
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w******e
发帖数: 1187 | 49 I tried to look up some kd info on RGD variant, but so far only
came up w/ this:
Modification of the amino acids
flanking the RGD sequence led to the synthesis
of EMD121974 (Cilengitide) inhibiting
aVb3 integrin binding to vitronectin with
an IC50 of 0.6 nM versus 900 nM for the
aIIbb3 integrin (Smith 2003).
I'm sure I read somewhere w/ more detailed description on peptide kd
(they are few and are b/w thou). I'll let u know when I come across
such info again.
one more thing, based on my even mor |
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C*******h
发帖数: 75 | 50 How do you claim a reduced expression level of your candidate genes in your
mutant? by qPCR or another approach? what's the control?
What is the size between two flanking markers? |
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