S**********e
发帖数: 1789 | 1 给你看看我们系刚进来的助理教授
Vanderbilt University School of Medicine
Fingleton, Barbara Mary , Ph.D. Assistant Professor of Cancer Biology
Publications
Barrett, CW, Fingleton, B, Williams, A, Ning, W, Fischer, MA, Washington, MK
, Chaturvedi, R, Wilson, KT, Hiebert, SW, Williams, CS. MTGR1 is required
for tumorigenesis in the murine AOM/DSS colitis-associated carcinoma model.
Cancer Res, 71(4), 1302-12, 2011.
Koon, HB, Fingleton, B, Lee, JY, Geyer, JT, Cesarman, E, Parise, RA, Egorin,
MJ, Dezube, BJ... 阅读全帖 |
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R*******o
发帖数: 19 | 2 Job Title: Senior Scientist position in Antibody department
Job Description：
1、Provide support for premium hybridoma relevant antibody projects,
customer communication from quotation to whole project management.
2、Be involved in internal antibody project design and technical support.
3、Provide supervisory and training to associate and assistant scientists.
4、Be responsible for research and development for new technology
development to promote our antibody services and products.
5、Working with m... 阅读全帖 |
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g*********5
发帖数: 2533 | 4 how to purify the antibody from Hybridoma cells? |
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d****i
发帖数: 2346 | 5 这个我想细胞不管用什么养，抗体一直是分泌的吧？
理论上似乎是细胞数目够了以后可以直接换，抗体浓度会高一点。
hybridoma |
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r******g
发帖数: 600 | 6 ebola大爆发的情况下，没有时间来进行hybridoma和screening～ 最直接的办法就是加
大产量，直接做serum iv治疗～
只要有效果，根本不用管他是什么药
ebola有几个subtype，下一次再大爆发的时候，搞不好这种serum就没有效果了，所以
做单抗也没有什么 manufacturing的意义～
就直接serum therapy挺好的
Good job！ Zapp Biopharm |
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a****y
发帖数: 47 | 7 A start-up Biotech located around LA is looking to hire a molecular
biologist with strong mAb sequencing and humanization experience. Please see
below for the details:
Position: research scientist for Molecule Biology
Major Responsibilities:
Responsible for determining mAb sequences from either hybridoma or from B
cells.
Responsible for humanization of mAb derived from mouse or rabbit.
Generate recombinant protein constructs for therapeutic proteins, or for
lab research.
Generate reporter syste... 阅读全帖 |
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b*******e
发帖数: 567 | 8 手头已有小鼠单克隆抗体的hybridoma cell line. 现在需要纯化出至少200mg的单抗用
来打小鼠模型做therapy研究。大家有啥廉价、高效的large-scale提纯单抗的方法吗？
多谢赐教！ |
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T*G
发帖数: 600 | 9 一般没事的，除非你的肿瘤用了lenti virus表达的又是oncogene，hybridoma 不清楚 |
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l*********2
发帖数: 247 | 10 怪就在怪在一些细胞复苏后看上去很好，但过一两天就死掉。我观察到的是悬浮细胞都
是死细胞，或者细胞碎片。 |
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h****6
发帖数: 229 | 11 主要看细胞在光镜下的型态。太多颗粒就不行了。
：怪就在怪在一些细胞复苏后看上去很好，但过一两天就死掉。我观察到的是悬浮细胞
都是死细胞，或者细胞碎片。 |
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l*********2
发帖数: 247 | 12 谢谢答复的两位。我前天看着细胞又要全军覆没，把细胞吹起来，洗后用预热的新培养
液重悬。现在细胞目前在长了，虽然很慢。 |
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l*********2
发帖数: 247 | 13 我也看过这个protocol，主要是复苏一开始要尽量高的细胞密度。 |
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t*x
发帖数: 1065 | 14 用feeder cell
3T3就行
10000rpm
了。 |
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r**a
发帖数: 121 | 15 各位，我以前实验室用的是hybridoma bank，9E10
挺好用的，但是用的dilution是1:5的样子，但是我们实验室自己有细胞系，所以无所
谓。
现在到了新实验室，要用这个抗体，大家推荐一下哪个公司的9E10 myc tag抗体好用啊
？Western和IF上应用！
谢谢啦！ |
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j****n
发帖数: 3370 | 16 单抗是不是主要由hybridoma或者mouse生产的？
人体免疫会不会一个问题？
虽然做antibody humanization的很多 这个问题解决到什么程度了？还是基本都解决了？ |
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v**********t
发帖数: 9 | 17 先附上猎头联系方式：qq3386366586，微信：futing2870
职位一：生物大分子发现总监（Phage Display）
岗位职责：
1. 负责构建人抗体的噬菌体展示库，利用展示技术进行新单克隆抗体序列的筛选、
鼠源抗体人源化、亲和力成熟以及抗体成药性的优化；
2. 负责除单克隆抗体外的其他新型结构抗体包括双特异抗体、单域抗体的噬菌体展
示筛选、优化；
3. 在创新生物大分子药物的研发过程中，协同其他专业团队，保证项目研发快速高
质量推进；
4. 协助调研临床疾病治疗需求，进行创新项目的选题立项；
5. 负责噬菌体展示实验室仪器设备、试剂耗材等的综合管理；理；
6. 负责相应团队的培训和建设、指挥与管理。
任职要求：
1. 学历要求：博士学历；
2. 专业要求：分子生物学、生物化学、免疫学等相关专业；
3. 工作经验：
1) 具有8年以上噬菌体展示技术开发生物大分子药物工作经验者；
2) 或具有5年以上国际大药企的噬菌体展示筛选部门成功... 阅读全帖 |
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z*******o
发帖数: 1794 | 18 一般还是hybridomas，但那个慢，要是must win的project两个会一起上。我本人不做
这个，这是我面试的时候一个group leader说的，还望业内人士指点。
[在 jasonhandang () 的大作中提到：]
:多谢指点，不知道这方面在药厂用的多不多？ |
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f***x
发帖数: 1524 | 19 hybridomas筛的是🐭源抗体，需要人源化。前期需要免疫动物。immune
response 是个问题。优点是有in Vivo quality control,一般不再需要affinity
Maturation and solubility/stability optimization.
相比较，display 可以从人工库筛，库可以很大(10e7-10e11)，用时短，不用考虑免疫
问题。但是往往需要affinity maturation.
Phage display 筛fragment.
yeast display可以 筛full length antibody.
方法上，phage display 简单。主要是Elisa and affinity binding/washing.
yeast display 用到FACS,可以对筛选有很好的控制，同时筛Antibody expression/
stability 和antibody-antigen binding. |
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z*t
发帖数: 863 | 20 受教了！您怎么看alpaca里搞得nanobody?
：hybridomas筛的是🐭源抗体，需要人源化。前期需要免疫动物。immune
：response 是个问题。优点是有in Vivo quality control,一般不再需要affinity
：Maturation and solubility/stability optimization.
：相比较，display 可以从人工库筛，库可以很大(10e7-10e11)，用时短，不用考虑免
疫问题。但是往往需要affinity maturation.
：Phage display 筛fragment.
：yeast display可以 筛full length antibody.
：方法上，phage display 简单。主要是Elisa and affinity binding/washing.
：yeast display 用到FACS,可以对筛选有很好的控制，同时筛Antibody expression/
：stability 和antibody-antigen binding. |
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M*******h
发帖数: 645 | 21 Scientific Support Specialist (Boston, MA)
Syd Labs, Inc in Boston mainly provides molecular biology and antibody-
related products and services. Syd Labs provides comprehensive services of
custom antibody development and applications in addition to a large number
of catalog antibodies (polyclonal, monoclonal, humanized, and modified) and
products for antibody production and applications, including peptides/amino
acids and derivatives, DNA constructs/vectors/extraction, proteins (natural,
recomb... 阅读全帖 |
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L******r
发帖数: 522 | 22 以前各个国家的情况其实不太一样，尤其是欧洲，但是现在基本统一了。就是如果你做
，不去卖（如果卖的地方有专利保护），这是属于R&D行为，基本没问题。在美国，
Waxman-Hatch还专门有个safe harbor section（35 U.S.C. §271. (e)(1)）来保护这
种行为，否则generic没法弄：
(1) It shall not be an act of infringement to make, use, offer to sell, or
sell within the United States or import into the United States a patented
invention (other than a new animal drug or veterinary biological product (as
those terms are used in the Federal Food, Drug, and Cosmetic Act and the
Act of March 4, 1913) which is primarily... 阅读全帖 |
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R*******o
发帖数: 19 | 23 目前有两个senior scientist 的猎头职位，分别从事抗体方向、抗体工程方向的药物
科研。
以下是简单JD供参考。需要详细了解的可联系私人邮箱r********[email protected]
【因为上论坛需要翻墙，所以比较少时间在线，咨询请邮件联系，请谅解】
Job Title: Senior Scientist position in Antibody department
Job Description
1、Provide support for premium hybridoma relevant antibody projects,
customer communication from quotation to whole project management.
2、Be involved in internal antibody project design and technical support.
3、Provide supervisory and training to associate and assistant scientists.
4、Be respons... 阅读全帖 |
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a****y
发帖数: 47 | 24 公司刚成立，国内投资人是资本背景，目前处于招兵买马阶段。有意者站内联系，具体
信息如下：
Chief Technology officer/VP of antibody engineering
Moreland therapeutics, a newly formed antibody therapeutics company in west
Los-Angeles area, is seeking a biotech industry veteran to serve as chief
technology officer of the company. Moreland therapeutics is funded by a
public traded company in China with the aim to deliver low cost biological
drugs to emerging market. The chief Technology Officer& vice president is
responsible for building ... 阅读全帖 |
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S****u
发帖数: 42 | 25 来自主题: Pharmaceutical版 - 无标题 Please apply from the Pfizer Website:
https://pfizer.wd1.myworkdayjobs.com/PfizerCareers/job/United-States---
California---Rinat/Scientist--non-PhD---Protein-Engineering_4692599
Pfizer -CID
Pfizer Cancer Immunology Discovery (CID) is a South San Francisco
biotechnology unit within the Oncology division of Pfizer Worldwide Research
& Development, dedicated to doing its part to fulfill Pfizer's mission of
working together for a healthier world. CID is dedicated to developing new
protein and cell-b... 阅读全帖 |
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h********0
发帖数: 944 | 26 Job Details
Requisition Number 13-0099
Post Date 12/6/2013
Title Antibody Development Scientist
City Gaithersburg
State MD
Description
POSITION SUMMARY:
The Antibody Development Scientist is responsible for development and
characterization of antibodies. Independently researching state of the art
methodologies and approaches for making antibodies, strategic thinking,
designing and executing experiments, analyzing resulting data and presenting
findings to the management will be among the... 阅读全帖 |
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h********0
发帖数: 944 | 27 Job Details
Requisition Number 13-0099
Post Date 12/6/2013
Title Antibody Development Scientist
City Gaithersburg
State MD
Description
POSITION SUMMARY:
The Antibody Development Scientist is responsible for development and
characterization of antibodies. Independently researching state of the art
methodologies and approaches for making antibodies, strategic thinking,
designing and executing experiments, analyzing resulting data and presenting
findings to the management will be among the... 阅读全帖 |
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