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全部话题 - 话题: insoluble
1 (共1页)
s********n
发帖数: 2939
1
来自主题: Military版 - 普鲁士蓝解毒机理
为什么一帮人就喜欢动嘴皮子不喜欢动手狗一下?明明说了是Fe4[Fe(CN)6]3,哪来的
钾?
https://en.wikipedia.org/wiki/Prussian_blue
Prussian blue
From Wikipedia, the free encyclopedia
This article is about the pigment. For the musical duo, see Prussian Blue.
See also: Midnight blue
Prussian blue
IUPAC name[hide]
Iron(II,III) hexacyanoferrate(II,III)
Other names[hide]
Berlin blue
Ferric ferrocyanide
Ferric hexacyanoferrate
Iron(III) ferrocyanide
Iron(III) hexacyanoferrate(II)
Parisian blue
Identifiers
CAS number 14038-43-8
PubChem ... 阅读全帖
f**********r
发帖数: 18251
2
来自主题: WaterWorld版 - Re: 普鲁士蓝解毒机理 (转载)
【 以下文字转载自 Military 讨论区 】
发信人: smartkevin (PE), 信区: Military
标 题: Re: 普鲁士蓝解毒机理
发信站: BBS 未名空间站 (Wed May 1 11:49:11 2013, 美东)
为什么一帮人就喜欢动嘴皮子不喜欢动手狗一下?明明说了是Fe4[Fe(CN)6]3,哪来的
钾?
https://en.wikipedia.org/wiki/Prussian_blue
Prussian blue
From Wikipedia, the free encyclopedia
This article is about the pigment. For the musical duo, see Prussian Blue.
See also: Midnight blue
Prussian blue
IUPAC name[hide]
Iron(II,III) hexacyanoferrate(II,III)
Other names[hide]
Berlin blue
Ferric ferrocyanide
Ferric hexacy... 阅读全帖
i********n
发帖数: 67
3
来自主题: pets版 - 为丁丁祈福
catinfo抄下来的
Soluble fiber traps water in the stool, thereby softening it, but it does
not swell as much as insoluble fiber. It is fermented into compounds that
can be helpful for colon health.
Insoluble fiber (e.g., cellulose from vegetables) also traps water but it
swells - adding bulk to the stool. However, this is not what we want for
cats. Cats are not designed to eat a high plant fiber diet that results in
a high volume (large diameter) stool.
Vegetables contain predominantly insoluble fi... 阅读全帖
c*******2
发帖数: 872
4
说实话, 我觉得是你的美国医生不熟悉中国食谱吧。 中餐本来相对纤维就比美国菜多
很多, 要不为什么在癌症的发病率里面, 美国排在前面的是肠癌。
fiber分两种, soluable fiber, 和insoluble fiber, soluble fiber, 前者帮助
排便和constipation, 后者主要是提供血糖缓冲, 降低胆固醇, insoluble主要是在
粗粮里面多, 有些比较硬和脆的蔬菜里面也有。 而soluble在蔬菜和水果里面都有。
所以combine soluble和insoluble的种类才是王道。 当然这都建立在个人tolerance上
面, 要是你吃了粗粮, 天天胀气, 肚子难受, 那就多吃些蔬菜水果了。
c*******2
发帖数: 872
5
说实话, 我觉得是你的美国医生不熟悉中国食谱吧。 中餐本来相对纤维就比美国菜多
很多, 要不为什么在癌症的发病率里面, 美国排在前面的是肠癌。
fiber分两种, soluable fiber, 和insoluble fiber, soluble fiber, 前者帮助
排便和constipation, 后者主要是提供血糖缓冲, 降低胆固醇, insoluble主要是在
粗粮里面多, 有些比较硬和脆的蔬菜里面也有。 而soluble在蔬菜和水果里面都有。
所以combine soluble和insoluble的种类才是王道。 当然这都建立在个人tolerance上
面, 要是你吃了粗粮, 天天胀气, 肚子难受, 那就多吃些蔬菜水果了。
h******r
发帖数: 279
6
来自主题: loseweight版 - 食品标签的问题
Total Carbohydrate 24g
dietary fiber 13g
soluble fiber 3g
insoluble fiber 10g
Sugars 8g
Other carb 3g
也就是说, 当我计算Net Carb的时候, 这个serving的Net Carb应该只减去10g的
insoluble fiber, 也就是14g. 我计算的对吗?
另外一种食品label写的
Total Carbohydrate 8g
dietary fiber 7g
Sugars 0g
这个的话, Net Carb怎么算呢?
v******s
发帖数: 6949
7
个人觉得insoluble fiber有点over market了。
好多老美喝insoluble fiber supplement吃粗粮,却很少吃蔬菜,太傻了。

v******s
发帖数: 6949
8
个人觉得insoluble fiber有点over market了。
好多老美喝insoluble fiber supplement吃粗粮,却很少吃蔬菜,太傻了。

g****r
发帖数: 306
9
来自主题: gardening版 - Fertilizer upon planting
果树用那种肥比较好。这是网上的一段:
Most people don't know that fruit trees need different fertilizer than other
types of trees. Most trees get a fertilizer with a 4-1-1 ratio of nitrogen,
phosphorus, potash. But giving that much nitrogen to a fruit tree will
encourage it to put out a tremendous flush of vegetative growth with very
few flowers and not much fruit.
A low nitrogen 1-1-1 or 1-2-1 ratio is better. Ideally, the nitrogen
component should be half water insoluble, or slow release, and half water
soluble.... 阅读全帖
w****j
发帖数: 5581
10
这是富兰克林写于1754年的话:
"It would be a strange thing if six nations of ignorant savages should be
capable of forming a scheme for such a union and be able to execute it in
such a manner as that is has subsisted ages and appears insoluble; and yet
that a like union should be impracticable for ten or dozen English colonies."
虽然这里面仍然充满西方人对北美印第安人的偏见,但可以看出来的是富兰克林是研究
过the Iroquois League的政治组织方式后写下的话。另外,国会在1775年由John
Hancock签署的对the Iroquois的讲话是这样说的:"The Six Nations (the Iroquois
League) are a wise people, let... 阅读全帖
m****n
发帖数: 226
11
来自主题: Military版 - 该囤点普鲁士蓝了
是口服胶囊
Radiogardase® (Prussian blue insoluble capsules)
http://www.heyltex.com/radiogardase.php
R******d
发帖数: 5739
12
a side note, not all particles will cause lung cancer, only the ones that
can go into your lung and insoluble by water. the reason asbestos can
deposit in a lung is because the particle size is so fine that it pass your
nose, which is itself a good filter and go all the way to a lung. most of
the particles in air are larger particles that gets filtered before going
into human lung

lung
l****z
发帖数: 29846
13
到底有多少同性恋?一项最新研究估计,1.7%美国成年人认为自己是同性恋,1.8%认为
是双性恋,8.2%自称曾有过同性性行为
SAN FRANCISCO (AP) — How many gay men and lesbians are there in the United
States? Gary Gates has an idea but acknowledges pinpointing a solid figure
remains an elusive task.
Gates is demographer-in-residence at the Williams Institute on Sexual
Orientation Law and Public Policy, a think tank based at the University of
California, Los Angeles. For the institute's 10th anniversary this week, he
took a scholarly stab at answering the qu... 阅读全帖
c**i
发帖数: 6973
14
来自主题: Food版 - Cellulose 纖維素
Sarah Nassauer, Why Wood Pulp Makes Ice Cream Creamier. Wall Street Journal,
May 4, 2011.
http://online.wsj.com/article/SB1000142405
2748703834804576300991196803916.html
Note:
(a) colloid
http://en.wikipedia.org/wiki/Colloid
(A hydrocolloid is defined as a colloid system wherein the colloid particles
are dispersed in water)
Chemically, a typical hydrocolloid is emulsion, where an individual particle
with an oil core is separated nd stabilized--so that the oil droplets will
not rise to the top--b... 阅读全帖
t*******n
发帖数: 4445
15
* Don't eat ONLY these 4, but a variety of foods. If your only veg is
seaweed it's probably low in vitamin C and insoluble fiber, for example.
* Eat everything in moderation, esp. the eggs.
D*****0
发帖数: 35
16
来自主题: NextGeneration版 - 孕妇维生素/钙片/牛奶一起吃没问题吧

刚好我最近也做了一些research,铁片要空腹吃,和钙片,牛奶一起会妨碍吸收,最好
橙汁(维他命C)送服。
http://en.wikipedia.org/wiki/Iron_supplements
“Iron forms an insoluble complex with several other drugs, resulting in
decreased absorption of both iron and the other drug. Examples include
tetracycline, penicillamine, methyldopa, levodopa, bisphosphonates and
quinolones.
The same can occur with elements in food, such as calcium. Absorption of
iron is better at a low pH (an acid environment), and resorption is
decreased if there is a simul... 阅读全帖
c*********o
发帖数: 1734
17
I will recommend the first three responds with the right background to the
editor.
There is no guarantee you will be picked by the editor.
Send email to c*********[email protected] with the following information:
You official name
Your degree
Your business email address
You business title
And brief introduction of your background.
Most important: register with EJPS as a reviewer with your business email
address before you send me email
I will not recommend you if you miss any of above.
"Solid self - e... 阅读全帖
m****n
发帖数: 75
18
感兴趣的朋友麻烦您回复站内邮箱,包括您的姓名,简要背景和email
谢谢!
c******a
发帖数: 2215
19
Sent.
Thanks.
g*********r
发帖数: 23
20
来自主题: Immigration版 - 借人气求ebook
借人气求ebook:
1.
Methods in Molecular Biology
Volume 824 2012
Recombinant Gene Expression
Editors: Argelia Lorence
ISBN: 978-1-61779-432-2 (Print) 978-1-61779-433-9 (Online)
http://link.springer.com/book/10.1007/978-1-61779-433-9
2.
Methods in Molecular Biology
Volume 1258 2015
Insoluble Proteins
Methods and Protocols
Editors: Elena García-Fruitós
ISBN: 978-1-4939-2204-8 (Print) 978-1-4939-2205-5 (Online)
http://link.springer.com/book/10.1007/978-1-4939-2205-5
[email protected]/* */
谢谢!
i*v
发帖数: 47
21
来自主题: Japan版 - 日本千老中的稳拿
JACS和Angew. Chem. Int. Ed. 一作8篇。。。。
回去的早可以评院士了吧。
Structural Elucidation and Regioselective Functionalization of An Unexplored
Carbide Cluster Metallofullerene Sc2C2@CS(6)-C82
X. Lu, K. Nakajima, Y. Iiduka, H. Nikawa, N. Mizorogi, Z. Slanina, T.
Tsuchiya, S. Nagase, T. Akasaka
J. Am. Chem. Soc. in press.
Crystallographic X-Ray Analyses of Yb@C2v(3) -C80 Reveal a Feasible Rule
That Governs the Location of a Rare Earth Metal inside a Medium-Sized
Fullerene
X. Lu, Y. Lian, C. Beavers, N. Mizorogi, Z... 阅读全帖
s****n
发帖数: 211
22
为了逃避审查, 不良厂商曾把苏丹红改过几十个名字.
Atul Orange R
Benzene-1-azo-2-naphthol
Brasilazina oil Orange
Brilliant oil Orange R
Calcogas M
Calcogas Orange NC
Calco oil Orange 7078
Campbelline oil Orange
Carminaph
Ceres Orange R
CerotinOrange G
CI Solvent Yellow 14
C.I. Solvent Yellow 14
Dispersol Yellow PP
Dunkelgelb
Enial Orange I
Fast oil Orange
Fast oil Orange I
Fast Orange
Fat Orange I
Fett Orange 4A
Grasal Orange
Grasan Orange R
Hidaco oil Orange
Lacquer Orange VG
MotiOrange R
Oil Orange
Oleal Orange R
Oran... 阅读全帖
A***u
发帖数: 3714
23
来自主题: Missouri版 - 我吃了海鲜
corticosteroid 阿,
吓我一跳。。。
***********************************************************************
Ultravate® contains halobetasol propionate, a synthetic corticosteroid
for topical dermatological use. The corticosteroids constitute a class of
primarily synthetic steroids used topically as an anti-inflammatory and
antipruritic agent.
Chemically halobetasol propionate is 21-chloro-6α, 9-difluoro-11β, 17-
dihydroxy-16βmethylpregna-1, 4-diene-3-20-dione, 17-propionate,
C25H31ClF2O5. It has the ... 阅读全帖
s********2
发帖数: 43
24
this sample had a red tint, which is not consistent with NS-2CVT oil, The
additive package does not match up with NS-2 CVT fluid either, Insolubles
read only a trace. The TBN read 1.4 and the TAN read 1.2. Both are normal
reading. Wear metals are average.
以上是尼桑给出的鉴定书,手写了一个不保修。找了他们经理,说报告不清楚,经理说
再和dealer shop交涉一下,这是不是在耍赖啊?
g*2
发帖数: 658
25
的确是,谢谢指出. Natural soy milk contains little digestible calcium as it
is bound to the bean's pulp, which is insoluble in humans. To counter this,
many manufacturers enrich their products with calcium carbonate available
to human digestion.
t*********l
发帖数: 844
26
今天收到NRA的信,原来禁铅子弹是别有用心,鸟根本不是铅子弹毒死的:
The real truth is that lead ammunition is an unlikely cause of the alleged
poisoning, because the metallic lead used in bullets and shot is relatively
insoluble in the digestive tract of birds of prey and scavengers. Scientific
studies have confirmed that it is very difficult to poison some birds with
lead, even by repeatedly feeding them lead shot mixed with food over time.
On the other hand, lead compounds found in legacy paint, gasoline,
insecticides and mic... 阅读全帖
n**m
发帖数: 7872
27
"Fats consist of a wide group of compounds that are generally soluble in
organic solvents and largely insoluble in water. Chemically, fats are
generally triesters of glycerol and fatty acids. "
上面的from wiki
fatty acid如果不能转化成TG就不能储存在体内。
我生化专业的。。。
m*******u
发帖数: 23
28
没办法,最简易可行的。 就高这么一个月,只要没永久性伤害。
soluble fiber 可帮助处理LDL,据说是这样,同时也吃 insoluble fiber,帮助清理
肠胃
山寨阿特金快3个星期了,少了2,3磅就停止了,维度也没任何变化,不上阿特进1期没
效果。 就不到一个月了,我急死了
g*****y
发帖数: 6325
29
来自主题: loseweight版 - 从周一开始atkins(Oct17)!
恩,还有就是 啃2个celery stroke, 便便不止需要soluble fiber, 也需要insoluble
fiber帮助肠胃蠕动,celery里的粗纤维很多的。
g*****y
发帖数: 6325
30
来自主题: loseweight版 - 从周一开始atkins(Oct17)!
恩,还有就是 啃2个celery stroke, 便便不止需要soluble fiber, 也需要insoluble
fiber帮助肠胃蠕动,celery里的粗纤维很多的。
m******i
发帖数: 9479
31
损失insoluble fiber。然后物料损失也不小,因为juicer出来的渣其实还有不少果汁
在里面
p*********l
发帖数: 26270
32
来自主题: loseweight版 - 刚才熬了一锅steel cut oat的粥
这个GI值低,比米饭对血糖的影响小,比米饭耐饿
具体热量,要看粥里面干燕麦放了多少
不管米饭还是燕麦或者窝窝头都主要是carbs,都要控制量
A 1/4-cup serving of Bob's Red Mill Steel Cut Oats is equal to 44 g, has 170
calories, 3 g of total fat, 29 g of total carbohydrates, 5 g of dietary
fiber, no sugars and 7 g of protein.
McCann's Steel Cut Oatmeal, has 150 calories per 1/4-cup, or 40 g serving,
with 2.5 g of total fat, 27 g of total carbohydrates with 3 g of dietary
fiber, 1 g of soluble and 2 g of insoluble fiber, no sugars and 4 g of
protein.
c*******2
发帖数: 872
33
精细碳水确实要少吃, 我从06年开始吃杂粮米饭, 1/2的白米, 其余都是黑米, 红
米, 糙米, 高粱, 苡仁, 大麦, 燕麦的mix, 加白米主要是为了使这个米饭不那
么难嚼, 感觉很好, 皮肤变好,大便通畅。在我怀老二的后期, 得了轻度的妊娠糖
尿病, 我上馆子一吃白米饭, 餐后血糖就呼呼的200多以上。 吃我自己做的这个杂粮
米饭,餐后血糖就很好, 一点问题没有。现在我家人在我带动下也自己mix这种养生米
吃了。 唯一缺点就是煮的时间长, 要多放水, 不然insoluble fiber太多, 嚼不动。
c*******2
发帖数: 872
34
精细碳水确实要少吃, 我从06年开始吃杂粮米饭, 1/2的白米, 其余都是黑米, 红
米, 糙米, 高粱, 苡仁, 大麦, 燕麦的mix, 加白米主要是为了使这个米饭不那
么难嚼, 感觉很好, 皮肤变好,大便通畅。在我怀老二的后期, 得了轻度的妊娠糖
尿病, 我上馆子一吃白米饭, 餐后血糖就呼呼的200多以上。 吃我自己做的这个杂粮
米饭,餐后血糖就很好, 一点问题没有。现在我家人在我带动下也自己mix这种养生米
吃了。 唯一缺点就是煮的时间长, 要多放水, 不然insoluble fiber太多, 嚼不动。
v******s
发帖数: 6949
35
一只手打字不给力呀
http://en.wikipedia.org/wiki/Phytic_acid
Phytic acid has a strong binding affinity to important minerals, such as
calcium, iron, and zinc, although the binding of calcium with phytic acid is
pH-dependent.[18] The binding of phytic acid with iron is more complex,
although there certainly is a strong binding affinity, molecules like
phenols and tannins also influence the binding.[19] When iron and zinc bind
to phytic acid they form insoluble precipitate and are far less absorbable
in the... 阅读全帖
l*****t
发帖数: 117
36
来自主题: loseweight版 - 脂肪从哪里开始烧 ?

是BBC专门拍的一部说减肥的纪录片。叫做: 10 Things You Need To Know About
Losing Weight.
youku地址:http://v.youku.com/v_show/id_XNTYyODM5MjE2.html
还是中文字幕。里面有各种减肥实验还有个案分析。
我贴一个总结:**但是还是建议看一遍这个片子, 挺好看的
1. Don’t skip meals
If you skip meals, your brain craves higher-calorie foods; and sub-
consciously you’re drawn more to the more fattening foods. They showed this
through MRI or CT scans of people who were hungry or not hungry reacting to
different foods. If you were hungry there was more activity in your brain
whe... 阅读全帖
D******l
发帖数: 359
37
to kill grubs in spring & fall:
http://www.homedepot.com/h_d1/N-5yc1v/R-202019111/h_d2/ProductD
(once it grows big, nothing can kill it. some people suggested overwatering
may drive grubs out.)
to prevent for next season (by killing new hatchs between june - august):
http://www.homedepot.com/h_d1/N-5yc1v/R-100619063/h_d2/ProductD
apply between april - june for best results (active ingredient is almost water insoluble, take time to
move into dirt)
Spectracide Triazicide Insect Killer will not ki... 阅读全帖
m****6
发帖数: 8689
38
太好了,明白了。
不过,网上居然显示我们local店里没有。
我的去亲自去看看,有没有小包装的,那么大袋,啥时用完啊。

overwatering
water insoluble, take time to
z*****e
发帖数: 14786
39
来自主题: gardening版 - 跪求指点!
精华区看了一遍,还是云里雾里 T_T
成分是这样的:
Total Nitrogen 6%
0.63% Nitrate Nitrogen
2.38% Ammoniacal Nitrogen
0.59% Other/ Water Soluble
0.08% Urea Nitrogen
2.32% Water Insoluble Nitrogen
Available Phosphate (P205) 6%
Soluble Potassium as (K20) 6%
Chlorine, not more than 2%
这样能看出门道吗?
F***I
发帖数: 4170
40
来自主题: gardening版 - 含有大量味精的天然食品
Monosodium glutamate
From Wikipedia, the free encyclopedia
Jump to: navigation, search
This article is about the chemical compound. For its use in food, see
glutamic acid (flavor). For glutamic acid in general, see glutamic acid.
Monosodium glutamate
Chemical composition of monosodium glutamate
Structure of monosodium glutamate
Crystalline monosodium glutamate
IUPAC name[hide]
Sodium 2-Aminopentanedioate
Identifiers
CAS number 142-47-2 Yes
PubChem 85314
ChemSpider 76943 Yes
UNII ... 阅读全帖
H********g
发帖数: 43926
41
来自主题: Joke版 - CO2
前几天在netflix上看了个TED,里面说今日地球上的铁矿,就是因为远古蓝细菌的作用
,把原本缺氧的水-大气圈搞成了氧化性的,结果把海里的二价铁氧化成三价铁沉淀了。
wikipedia上这么说:
Banded iron formation
Some of the oldest known rock formations, formed over 3,700 million years
ago, include banded iron layers.[2] Banded layers rich in iron were a common
feature in sediments for much of the Earth's early history but are now rare
.[citation needed] Phanerozoic ironstones generally have a different genesis.
The conventional concept is that the banded iron layers were formed in sea... 阅读全帖
H********g
发帖数: 43926
42
来自主题: Joke版 - 术版说说,蟹黄能吃吗
实际上真正爱吃虾蟹肝脏(听说抗癌明星虾蟹先生已经和肝毒斗争很久了)是西人,他
们用龙虾的肝脏做酱。
这个卫生官员可能是只懂西餐,不大懂中餐,所以张冠李戴,认为中文里说的蟹膏和
crab fat/lobster paste/Tomalley 是一回事。
=========================
http://en.wikipedia.org/wiki/Tomalley
The hepatopancreas of a crab is also called tomalley, or crab "fat"; in
crabs the tomalley is yellow or yellow-green in colour.[2][3][4] In Maryland
and on the Delmarva Peninsula, the hepatopancreas of the blue crab is
called the "muster" or "mustard", probably because of the yellow colour,
which is not the b... 阅读全帖
i********n
发帖数: 67
43
来自主题: pets版 - 为丁丁祈福
我没记错的话南瓜好像也可以起到反作用 猫便秘insoluble fiber弊大于利
T*****n
发帖数: 2456
44
来自主题: Belief版 - 第五节 宗教大法官
THE BROTHERS KARAMAZOV
By Fyodor Mikailovich Dostoevsky
Translated by Constance Garnett
Chapter 5
The Grand Inquisitor
https://www.mtholyoke.edu/acad/intrel/pol116/grand.htm
"EVEN this must have a preface- that is, a literary preface," laughed Ivan,
"and I am a poor hand at making one. You see, my action takes place in the
sixteenth century, and at that time, as you probably learnt at school, it
was customary in poetry to bring down heavenly powers on earth. Not to speak
of Dante, in France, cle... 阅读全帖
p***l
发帖数: 134
45
大主教在达尔文自传上面闹的是大乌龙,或者是故意的断章取义。现在已知有无数的文
献证据证明达尔文晚年是极为坚定的无神论者。如果任何人竟然宣称达尔文其实是信
徒的话,像这位主教一样,他一定是自取其辱。造谣的霍普修女和大量的基督教传教小
册子就是先例。
事实上达尔文自传首先描述了自己早年对基督教的信仰——达尔文成长于基督教家庭,
接受过完整的基督教教育,甚至是以传教士身份登上了贝格尔号——如果达尔文早年的
思想没有一点基督教的印记,那倒是怪事了!然后话锋一转,立刻就说到“I for one
must be content to remain an Agnostic”。这种“将欲夺之,必先予之”的笔法是
达尔文常用的修辞手法。比如他对眼睛进化过程的描述:他首先说眼睛的复杂程度使得
进化论几乎无法解释其成因,然后话锋一转,紧接着再把眼睛进化的过程娓娓道来。可
惜达尔文的这种修辞手法为宗教家提供了断章取义的空隙。比如这位Pell主教,既然连
引用页数都记得清清楚楚,难道他会不知道紧接下来的一段就是对他的直接驳斥吗?对
于这种早有预谋的资料搜集,难怪Dawkins也只能叫唤几句“That's not... 阅读全帖
r****n
发帖数: 4
46
Good point!
I am sure that the precipition was caused by potassium ions which complex with
SDS and become insoluble. You should remove potassium ions from your SDS
extraction solution by using sodium salt.
I do think your protein concentration is too high because I even used much
higher protein concentration for my protein 2d electrophoresis. But for
SDS-PAGE, you can not load too much protein even with a concentrated sample.
You still have to dilute your sample. I usually load 20-30 ug for my
C******n
发帖数: 941
47
the more harsh your solution, the more components will disolve, of course the
more you will see on the gel.
If you want to disolve all the pellet, try the most "harsh" solution you can
imagine, like 5%-Triton-2%SDS-8M urea.....
C******n
发帖数: 941
48

interfere
~~~~~~~~~~~~~~~~~~~~~~~~ this is not harsh to
disolve
all proteins, like some membrane proteins, they are really hard to disolve.
High concentration of TritonX-100 will affect SDS-PAGE, you can try 8M urea,
or you can even add 8M urea when casting the SDS-Gel, search methods with key
words like" disolve membrane proteins", "membrane protein SDS-PAGE", you'll
get more informative stuff.
Good luck

can
buffer
s********n
发帖数: 2939
49
从current protocol上改的:
1. check OD600;
2. harvest X ul induced or uninduced culture;
3. add Y ul 1x SDS-PAGE loading buffer (Y=OD600*X/10);
4. Vortex for 30s;
5. 100C for 5 min;
6. 15,000x g for 5 min;
7. Load 5-20 ul supernatant to SDS-PAGE gel.
你也可以向楼上所说,把soluble和insoluble的fractions分离后再SDS-PAGE.
p********i
发帖数: 116
50
来自主题: Biology版 - chaperone表达位置
最近发现一个蛋白A,可以另外一个蛋白B binding并且影响B的soluble和insoluble的
分布,但是B的总量
不变,感觉A是不是chaperone, 但是做染色和ER的marker没有在一起定位,这就能说明
这个不是个
chaperone了吗?
1 (共1页)