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全部话题 - 话题: isotope
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e****p
发帖数: 354
1
不想接触ISOTOPE,但听说其他chemiluminescence 背景太强,灵敏度不高。。。
有什么可以推荐的盒子,谢谢!
x*********i
发帖数: 123
2
来自主题: Chemistry版 - 有没有做Stable Isotope的
Cambridge Isotope Lab CIL?
y*****s
发帖数: 1047
3
来自主题: Chemistry版 - 有没有做Stable Isotope的
what is "做Stable Isotope"?
a********m
发帖数: 18
4
来自主题: Chemistry版 - 有没有做Stable Isotope的
不好意思,没讲清楚, 是指做Stable Isotope Labeling。
t***h
发帖数: 5601
5
来自主题: Military版 - 关于朝鲜2013年核试验的报道
http://www.ctbto.org/the-treaty/developments-after-1996/2013-dp
Media questions and answers on radionuclide detection
Vienna, 23 April 2013
1. How come the detection was made so late (when you said on 12 March that
it was improbable)?
A: The main detection was made on 8 and 9 April, a smaller one from 12 to 14
April. Detection of radioactive noble gas more than 7 weeks after an event
is indeed unusual, we did not expect this and it did not happen in 2009. We
do not know what has happened at the ... 阅读全帖
z****g
发帖数: 2497
6
来自主题: Java版 - Help! ClassCastException
I got a very weird exception
code:
...
//isotopes is a Vector
cdk.Isotope iso = (cdk.Isotope)isotopes.elementAt(0);
....
when it runs this line, it throws a ClassCastException
But when I printout isotopes.elementAt(0).getClass().getName()
it print out "cdk.Isotope".
Can't figure out why.
Please help!
s*********8
发帖数: 901
7
来自主题: Military版 - Iran top nuke scientists murdered
TEHRAN, Iran – Assailants on motorcycles attached magnetized bombs to the
cars of two nuclear scientists as they were driving to work in Tehran on
Monday, killing one and wounding the other, Iranian officials said. The
president accused Israel and the West of being behind the attacks.
Iran's nuclear chief, Ali Akbar Salehi, said the man killed was involved in
a major project with the country's nuclear agency, though he did not give
specifics. Some Iranian media reported that the wounded scientis... 阅读全帖
w*********g
发帖数: 30882
8
平井宪夫揭示日本核电业惊天内幕——素人造核电,豆腐渣工程
作者:蜀中行 更新时间:2011-3-16
设施配管1级技士平井宪夫(1997年1月因癌症逝世)生前的最后吶喊。
/来自中华网社区 club.china.com/
/来自中华网社区 club.china.com/
关于平井宪夫:
1997年1月逝世。设施配管1级技士,核电事故调查国民会议顾问,核电劳工救济中
心代表,北陆电力志贺核电厂停止诉讼原告特别辅佐人,东北电力女川核电停止诉讼原
告特别辅佐人,福岛二号核电厂三号机运转停止诉讼原告证人。「核电劳工救济中心代
表」在他仙逝后,因后继无人关闭
"我不是反核运动家。这20年来,我一直在核电厂工作。社会上有听不完的拥核反
核理论,但我只想在这里告诉大家:「所谓的核电厂是这样一回事。」大部份的人都不
知道核电内部的实际情形。希望大家有耐心把这篇文章读完。所谓核电,跟各位所想的
或许有点出入。在那里,每天都有遭受放射线污染的工人,以及严重的岐视产生。
我的专长,是负责大型化学工场的内部配管施工与维修。快30岁时,日本掀起了一
阵核电建设的... 阅读全帖
h*******g
发帖数: 2201
9
唐教授是很不地道的.Nobody should be doing this.She should be kicked out of
her research field because this violation. She does not have the basic
humanity to be a scientist, even more shameful to do such thing to her own
race, like a 外F.
deuterium 对细胞的新陈代谢会产生很大的副作用(非放射同位素毒性),如果你是做NMR
,你会用E Coli表达蛋白, E Coli需要很长的时间才能适应deuterated water because
of the isotopic mass of deuterated water is significantly different than
proton water.
check here:
http://en.wikipedia.org/wiki/Heavy_water
Heavy water is not radioac... 阅读全帖
p***n
发帖数: 17190
10
https://www.upmedia.mg/news_info.php?SerialNo=69079
俄測試飛彈推進器爆炸釀5死 輻射值飆升憂車諾比核災再現
讚 80
分享 用LINE傳送
麥浩禮 2019年08月10日 17:35:00
SHAREgoogletwitterlinkedin
媒體小農
俄羅斯西北部海軍基地發生爆炸,並洩出輻射物質。(湯森路透)
俄羅斯西北部阿爾漢格爾斯克(Nyonoksa)海軍基地8日發生爆炸及輻射洩露事故。
研究人員對飛彈推進器進行測試時,疑似操作不當發生爆炸,造成5名研究人員死亡、3
人受傷,同時出現短暫輻射洩露事故,但俄羅斯國防部仍拒絕透露細節。
BBC News (World)

@BBCWorld
Russia explosion: Five confirmed dead in rocket blast https://bbc.in/
2yNt1a0
Russian cruise missile test - file pic
Five confirmed dead in Russia rocket explosion
T... 阅读全帖
G*******g
发帖数: 556
11
来自主题: PhotoGear版 - 对这个核泄露怎么看呢?
Yes, the radiation will last long, but I am talking about its impact on
human health. I am not talking about dose here, but the dose RATE, which is
different from dose itself. It represent to a very large extend the actual
damage a radiation will do to human body. The damage that radiation can do
to a human body depends on the accumulative does, but more significantly on
dose rate, that is the dose that you receive per second. The activity can be
low, but the pollution duration can be long, they... 阅读全帖
H********g
发帖数: 43926
12
1)If the rock becomes molten, as happens in Earth's mantle, such
nonradioactive end products typically escape or are redistributed.[19] Thus
the age of the oldest terrestrial rock gives a minimum for the age of Earth,
assuming that no rock has been intact for longer than the Earth itself.
2)Most geological samples from Earth are unable to give a direct date of
the formation of Earth from the solar nebula because Earth has undergone
differentiation into the core, mantle, and crust, and this has t... 阅读全帖
h********g
发帖数: 143
13
Thank you very much!
Are you talking about the triplet of CDCl3 in C-NMR?
Actually, I don't think it is due to C-CL coupling.
I searched the triplet of CDCl3 in C-NMR and found this:
http://u-of-o-nmr-facility.blogspot.com/2007/09/isotope-shifts-
The CDCl3 signal is a 1:1:1 triplet due to the J coupling to the deuteron
which is a spin I=1 nucleus having three energy levels. The chemical shift
difference between the CHCl3 and CDCl3 isotopomers is called an isotope
shift.
Question 1: Since chlorin... 阅读全帖

发帖数: 1
14
自抽嘴巴----评孙某的青铜器同位素一文 (2016-03-21 16:45:04)转载▼
先普及一下基本术语:
铅同位素: 自然界中铅以204Pb、206Pb、207Pb、208Pb四种同位素的存在,相对丰度
分别为1.48%、23.6%、22.6%、52.3%。除204Pb(普通铅)为非放射成因外,其他几种
铅同位素是由其他元素衰变而来,具有放射性。206Pb和207Pb分别是铀的238、235衰变
,208Pb是钍232衰变产生。孙的文章就是拿207Pb/204Pb, 206Pb/204Pb两个比值进行几
个地点的对比。
(随带说一下,其文中Figure 1标题竟然还能打错成260Pb :
Figure 1: 260Pb/204Pb versus 207Pb/204Pb diagram for ancient bronzes2,7,9,58
,59 and ore deposits60 from China.)
铅同位素测量:研究铅同位素丰度变化时,以204Pb作为比较基础,测定其他各同位素
与204Pb的比值。孙的文章主要研究铀衰变的两种铅同位素206Pb、207Pb与普通... 阅读全帖
W***n
发帖数: 11530
15
Fukushima Radiation Found in Pacific Off California’s Coast
By Jacob Adelman Nov 10, 2014 8:31 PM CT
A fisherman casts his line along the Pacific ocean at sunset in San
Francisco.
Oceanographers have detected isotopes linked to Japan’s wrecked Fukushima
nuclear plant off California’s coast, though at levels far below those that
could pose a measurable health risk.
Volunteer ocean monitors collected the samples that tested positive for
trace amounts of the isotope cesium-134 about 100 miles (160 ... 阅读全帖
w******t
发帖数: 16937
16
Radon is a chemical element with symbol Rn and atomic number 86. It is a
radioactive, colorless, odorless, tastelessnoble gas, occurring naturally as
the decay product of uranium or thorium. Its most stable isotope, 222Rn,
has a half-life of 3.8 days. Radon is one of the densest substances that
remains a gas under normal conditions. It is also the only gas under normal
conditions that only has radioactive isotopes, and is considered a health
hazard due to its radioactivity. Intense radioactivity... 阅读全帖
G**m
发帖数: 138
17
给你两个我的paper 被heavily cited的例子. 我的citation非常少(英文citation<50
),所以这些正是我用来突出自己citation的地方。
“Another study used an ablation technique to make conclusions concerning
rates of diffusion and therefore disorder at grain boundaries. In this
technique layered samples with regions of isotopically heavy water are
ablated. The ablated material is analyzed using a mass spectrometer and the
diffusive profile around the isotopically heavy regions is correlated with
the annealing time. Such measurements provide a... 阅读全帖
R*R
发帖数: 2661
18
Another potential worry: The makeup of the radioactive material being leaked
by the plant has changed. Buesseler said the initial leak had a high
concentration of cesium isotopes, but the water flowing from the plant into
the ocean now is likely to be proportionally much higher in strontium-90,
another radioactive substance that is absorbed differently by the human body
and has different risks. The tanks (on the plant site) have 100 times more
strontium than cesium, Buesseler said. He believes t... 阅读全帖
G*******g
发帖数: 556
19
来自主题: PhotoGear版 - 对这个核泄露怎么看呢?
yes, the mSv/hr is high right now, but it is mainly caused by the short-life
isotope, because compared to these shor-life isotopes, the long-life ones
only contribute very little. After they decay away, the does rate
contribution from the long-life isotopes will be ordres lower than what is
being measured right now. That is my point.

like
of
R******d
发帖数: 5739
20
来自主题: Joke版 - 这就是变暖教的科研水平
我不是什么大牛, 这个也不是我专业。 但我相信要真的去较真, 你必须要有一定的
科学基础和专业知识。 这里大部分人可能没有专业知识, 质疑是对的, 但较真的话
水平可能不太够
quote
-------
II. The Vostok Ice-Core
To demonstrate the methods used in dating ice-cores I will use the Vostok
ice-core as an example because I found plenty of literature on it and
because it is an Antarctic ice-core which was what the original post was
about.
How It Was Collected
The Vostok Ice-Core was collected in East Antarctica by the Russian
Antarctic expedition. The Vostok Ice-Core is 2,083 meters lo... 阅读全帖
R******d
发帖数: 5739
21
来自主题: Joke版 - 这就是变暖教的科研水平
我不是什么大牛, 这个也不是我专业。 但我相信要真的去较真, 你必须要有一定的
科学基础和专业知识。 这里大部分人可能没有专业知识, 质疑是对的, 但较真的话
水平可能不太够
quote
-------
II. The Vostok Ice-Core
To demonstrate the methods used in dating ice-cores I will use the Vostok
ice-core as an example because I found plenty of literature on it and
because it is an Antarctic ice-core which was what the original post was
about.
How It Was Collected
The Vostok Ice-Core was collected in East Antarctica by the Russian
Antarctic expedition. The Vostok Ice-Core is 2,083 meters lo... 阅读全帖
z********i
发帖数: 610
22
来自主题: Biology版 - 有做glucose transporter的吗?
细胞是hela,glucose转运用glucose meter测的medium中glucose的含量,没有用
isotope。但是glucose代谢用的是isotope 3H标记的glucose,测isotope释放。
E****F
发帖数: 10
23
来自主题: Chemistry版 - 做Mass Spectrometry 的大牛请进
你的ion-trap MS/MS用了47eV,得到的product ion 信息不是很充足。如果要做Q-TOF的
话,可以尝试不同的collision enrgy,建议加大CE到60-100eV试试。另外扩大mass
range来看看是否有dimer或trimer. 如果猜测是脂肪酸的话,可以去www.lipidmaps.
org查询一下database,那里有isotope distribution for natural isotopes. 如果不
用NMR,就麻烦些上stable isotope, like C13, H2,or O18,希望对你有帮助。
p******s
发帖数: 137
24
三篇在此。。。
1. Title: A COMPARATIVE-STUDY ON OPTICAL EFFECTS OF E-CENTER IN QUARTZ
GRAINS FROM LOESS AND FAULT
Author(s): JIN SZ, DENG Z, HUANG PH
Source: APPLIED RADIATION AND ISOTOPES Volume: 44 Issue: 1-2 Pages:
175-178 Published: JAN-FEB 1993
Times Cited: 2

2. Title: THE IMPROVED SCHEMATIC BAND MODEL FOR ESR AND TL DATING
Author(s): DENG Z, HUANG PH, JIN SH
Source: APPLIED RADIATION AND ISOTOPES Volume: 44 Issue: 1-2 Pages:
287-291 Published: JAN-FEB 1993
Times Cited: 0

3.
w*********r
发帖数: 42116
25
Molecular laser isotope separation(MLIS)还是Atomic vapor laser isotope
separation (AVLIS)?
难度的顺序是氢、硼、氮、碳、氯、硫、钠、锂、溴、钙、钡、锇、铀,
90年代大陆就有激光分离硼的文献报告. 不知道有没有进展.
美国可以分离铀, 其它国家的报道没看到过.
y******u
发帖数: 246
26
LZSB
iodine片不是防止直接辐射,而是是防止人体吸收核反应产生的特殊isotopes,这些isotopes被吸
收后,会长期在人体内部辐射(>30年)。
h********n
发帖数: 5
27
来自主题: Military版 - 铊的同位素分析也许有助证据
在看东方卫视2003年的有关朱令案件的视频时,陈振阳教授提到两个峰,判断有两次中
毒。学过大学普通化学,以及生物等学科都知道,同一液态样品,同一物质的叠加一般
只能表现在浓度上,不表现在时间上,除非是一些放射性衰变的物质。如大家都听过但
并不见得熟悉原理的碳十四测化石年代法。当然陈是专家,他们的方法原理可能不同,
有人也说是取头发样,没有更多信息,不做评论。
上网查了一下铊的同位素isotope ,http://en.wikipedia.org/wiki/Thallium
有多种同位素,其中稳定的有铊203,和铊205,放射性的有铊201(半哀期72小时)铊
202(半衰期12天)铊204(半衰期3.8年。放射性的铊须特别制备,须高能物理方法,
非化学方式能得到。不知在九十年代中国有没有铊这方面的研究。
稳定的铊203 和205在地球上的平均自然丰度natural abandance一般是29.5:71.5.
http://www.webelements.com/thallium/isotopes.html
但是因为地理,来源矿源不同,丰度有些偏差。 http://eprints.so... 阅读全帖
t***h
发帖数: 5601
28
来自主题: Military版 - 关于朝鲜2013年核试验的报道
http://www.ctbto.org/press-centre/press-releases/2013/ctbto-det
CTBTO detects radioactivity consistent with 12 February announced North
Korean nuclear test
Vienna, 23 April 2013
The CTBTO's radionuclide network has made a significant detection of
radioactive noble gases that could be attributed to the nuclear test
announced by the Democratic People's Republic of Korea (DPRK) on 12 February
2013.
The detection was made at the radionuclide station in Takasaki, Japan,
located at around 1,000 kilome... 阅读全帖
p*r
发帖数: 5516
29
来自主题: Military2版 - Huge blast at Japan nuclear power plant zz
http://www.bbc.co.uk/news/world-12723092 (附图)
The word "meltdown" goes to the heart of the big nuclear question - is
nuclear power safe?
The term is associated in the public mind with the two most notorious
accidents in recent memory - Three Mile Island, in the US, in 1979, and
Chernobyl, in Ukraine, seven years later.
You can think of the core of a Boiling Water Reactor (BWR), such as the ones
at Fukushima Daiichi, as a massive version of the electrical element you
may have in your kettle.
It s... 阅读全帖
e*******6
发帖数: 72
30
有机合成研究员-有机金属(Organometallic Chemist)
职位性质:全职(Full time)
工作地点:纽约长岛(Long Island, NY)
学历要求:硕士(M.S.)
专业要求:有机化学 (Organic Chemistry)
岗位指责:
1、负责合成路线的制定,实验设计与反应条件优化;
2、对工艺流程和产品制造提供技术支持;
3、独立操作和维护实验器材和仪器;
4、分析和阐述项目中出现的问题,提出合理的改进建议;
5、带领多名实验助理开展研究工作。
任职资格:
1、有机化学硕士及以上学历,有机合成经验,熟悉对空气敏感的有机金属化合物的制
备与处理;
2、熟悉有机合成理论,能独立设计合成路线,有较强的英文文献查找和阅读能力;
3、熟练掌握有机合成、工艺优化、产物分离与结构鉴定技能,有色谱分析技术的优先;
4、积极上进,具备良好的团队合作精神和沟通能力;
5、具备优秀的英语听说读写能力。
有机合成研究员-同位素标记(Synthetic Chemist-Isotope Labeling)
职位性质:全职(Full time)
工作地点:纽约长岛(Long Isla... 阅读全帖
a**********y
发帖数: 20
31
来自主题: JobMarket版 - 招聘化学专业人才,办理H1签证
高级有机合成研究员(Senior Scientist – Synthetic Chemist)
职位性质:全职(Full time)
工作地点:纽约长岛(Long Island, NY)
学历要求:硕士(M.S.)
专业要求:有机化学 (Organic Chemistry)
岗位指责:
1、从事新化合物的设计和合成,或临床化合物和上市药物的合成和工艺优化;
2、根据课题安排,合理设计实验方案并仔细实施;
3、独立承担高难度课题任务,保证按时完成课题任务;
4、科学分析实验中出现的问题,提出合理的改进建议;
5、能够独立进行有或无文献报道详细实验操作的目标化合物的合成;
任职资格:
1、美国本土高校毕业,有机合成领域硕士及以上学历;
2、熟练运用NMR,FT-IR,UV-Vis,LC-MS等分析手段和方法;
3、一年以上有机合成化学研发工作经验;
4、工作态度踏实,能适应紧张的工作,有较强的执行力;
5、责任心强,具备良好的团队合作精神,可带领多名实验助理开展研究工作;
6、优秀的英语听说读写能力。
药物化学研究员(Medicinal Chemist)
职位性质:全职(Full time... 阅读全帖

发帖数: 1
32
橡树岭国家实验室散裂中子源诚招有物理和计算背景的博后。
课题为运用高性能计算增强非弹性散射中子谱仪的分辨率。年薪约$65k。
https://recruiting.ornl.gov/sap/bc/webdynpro/sap/zornl_hrrcf_c_post_doc?sap-
language=EN&sap-client=010#
Job Posting Title
Postdoctoral Research Associate in Super-Resolution Inelastic Neutron
Scattering
Purpose
The Neutron Sciences Directorate (NScD) at Oak Ridge National Laboratory (
ORNL) operates the High Flux Isotope Reactor (HFIR), the United States'
highest flux reactor based neutron source, and the Spallation Neutron Source
(... 阅读全帖
h****r
发帖数: 2056
33
Japan nuclear alert at Fukushima - Q&A
15 March 11 12:19 GMT
There have been two explosions at the Fukushima Daiichi nuclear power plant
in Japan, following Friday's earthquake and tsunami, and a third reactor is
reportedly at risk of fuel-rod meltdown.
How great a danger do these problems pose for people in Japan and further
afield?
Has there been a leakage of radioactive material?
Yes. Local government officials in Fukushima say 190 people have been
exposed to some radiation. An American warsh... 阅读全帖
i*****s
发帖数: 15215
34
来自主题: CanadaNews版 - 温哥华断脚案新闻汇总
Linking severed feet to missing persons a challenge, experts say
Last Updated: Thursday, June 19, 2008 | 6:32 PM ET
by Paul Jay CBC News
The disturbing discovery on Monday June 16 of the remains of a left foot in
a running shoe in the water near Westham Island in Ladner, B.C., may not
seem like much to go on for forensic investigators.
But the remains, one of five feet discovered in the last year and a half in
the same region, could potentially contain deoxyribonucleic acid (DNA), bone
minerals ... 阅读全帖
j*****n
发帖数: 3617
35
Albuquerque Isotope catcher Miguel Olivo bit off part of teammate Alex
Guerrero's ear during an altercation between the two players at Tuesday's
Isotopes game, Guerrero's agent Scott Boras said.
学泰森了?
e****i
发帖数: 2152
36

The ratio between the stable isotopes of carbon (12C/13C) seems to indicate
that significant changes in the carbon cycle took place starting about 500,
000 to 1 million years before the end of the Permian Period and crossing the
boundary into the Induan Age (the first age of the Triassic Period). These
changes appear to coincide closely with two Permian extinction events,
suggesting some cause-and-effect relationship with changes in the carbon
cycle.
Several studies have suggested that changes ... 阅读全帖
y****e
发帖数: 71
37
Make sure the dose you are handling and the type od radiation invilved.
In biology, usual isotope are not very harmful, because of the above 2
reasons.
such as H3, soft gamma ray, easy to absorb even in the air, usually do not
need to worry unless you eat or drink it.
P32 is beta, stronger than H3, sometimes some protection if dose is high.
Most time do not, if dose is low. Be careful of ingestion or other type ir
internal radiation.
S35 is also soft, easy to prevent.
I125 or other I isotope mi
h******n
发帖数: 493
38
来自主题: Biology版 - Re: 请教Mass Spectrometry的软件
There is no such software. You can calculate the molecular
weight by yourself, then add 1Da (H+) to it. That is what you
usually expect to see from MS analysis. Sometimes, you will
see sodium adduct, instead of proton adduct. In that case,
you have to add 23Da to the molecular weight.
There are some programs to calculate the isotopic pattern
for a molecule. That can help you a little. Just search
"isotopic pattern" in google and you should be able to
find quite a few.
c******r
发帖数: 3778
39
非常对。
这种传统方式的screening其实很不经济。
从老板的角度来说,有几个原因应该选择high throughput screening methods:
1. time。多数时候这些project都有competition。时间是决定这篇paper发cell还是
pnas,甚至进垃圾堆的决定性因素。而手动screening是无论如何无法与high
throughput的方法比的。
2. economy。选择比较成熟的high throughput的方法的话,使用的人很多,说明经济
上是比较合算的,大家都不是傻瓜,都很在乎钱。比如说microarray,其实做一次才几
百块。而isotope,一瓶也要上百。如果需要超过5瓶的isotopes,那就不合算了。
3. big picture。你做northern,不会想把所有基因都做一遍吧?如果发现了一个有意
思的是不是就停止了?而high throughput的方法会screen所有的基因,保证你不会
miss掉一个更有意思的。
4. sensitivity。northern很不敏感。靠band的深浅能分辨的差别需要很大才行。而
... 阅读全帖
z*t
发帖数: 863
40
来自主题: Biology版 - 同一期cell上两篇文章
说不上啥经验,大家一起看着玩玩:)
还有Micheal Synder哪篇Chia-PET,这个mutil-gene transcriptional cluster虽然他
们之前的paper已经这个意思,但这篇文章后肯定会有一批functional的study的。
Maria A. Blasco的muscle stem cell也很好玩,immortal strand这个hypothesis虽然
很扯,但无数人用现有的DNA label技术一直看到这个现象确实很难理解。这个可以和
最新的一篇nature isotope 成像的文章一起看,他们的isotope DNA label更可靠一点
l**********1
发帖数: 5204
41
Continue:
SULAQ
//www.ncbi.nlm.nih.gov/pubmed/22106033
Proteomics. 2012 Jan;12(1):37-
Sulfur-36S stable isotope labeling of amino acids for quantification (SULAQ).
>Abstract
>We introduce a universal metabolic labeling strategy using elemental heavy
36Sulfur (36S) called 36Sulfur stable isotope labeling of amino acids for
quantification (SULAQ). In the proof of principle experiment, Pseudomonas
putida KT2440 was grown in defined minimal medium with sodium benzoate or
sodium succinate as the sole... 阅读全帖
B****n
发帖数: 35
42
We have a dumb graduate student in our lab. Yesterday he received fresh 32p
isotope, 0.22 GBq, ATP, [γ-32P]- 6000Ci/mmol 150mCi/ml Lead, 5 mCi. He
opened the lead container and took the small bottle out just in front of me
without any shield and told me how strong this amount of isotope he gonna
use. I was working and didn't realize what he was doing. Later on, I
realized what happened and became so nervous and worried a lot, I coughed a
lot and even wanna to vomit this morning. I think I had ex... 阅读全帖
l**********1
发帖数: 5204
43
Firstly stable isotope 15N label of that protein then inert cell,
after 72 hrs
add synthetic macromolecules to mimic the crowded cytoplasm
next extract nuclei protein
the residue is cytoplasm protein
next do mass spectrometry individually
References:
1,
//www.isotope.com/uploads/file/prot_brochure_lysineupdatelores.pdf
2,
PLoS One. 4:e7560. |(2009)
Isolation of cell nuclei using inert macromolecules to mimic the crowded
cytoplasm.
http://www.ncbi.nlm.nih.gov/pubmed/19851505
y***e
发帖数: 6082
44
来自主题: Chemistry版 - 最全的化学数据库(zz)
【 以下文字转载自 NanoST 讨论区 】
发信人: maodouzi (毛豆子), 信区: NanoST
标 题: 最全的化学数据库(zz)
发信站: BBS 未名空间站 (Mon Mar 26 11:27:29 2007), 转信
1. 化合物毒性相关数据库
Toxnet http://toxnet.nlm.nih.gov/
2毒性物质与健康和环境数据库 http://esc.syrres.com/efdb/TSCATS.htm
3. 急性毒性数据库 http://www.cerc.usgs.gov/data/acute/acute.html
4. SpectraOnline,Galact http://spectra.galactic.com/SpectraOnline/Default_ie
.htm
5. 药物使用指南,USP DI http://www.nlm.nih.gov/medlineplus/druginformation.ht
ml
6。美国常用药物索引库RxList http://www.rxlist.com/
7. 有机化合物光谱资料库系统 http://w... 阅读全帖
a***a
发帖数: 5
45
来自主题: GeoSpace版 - 请教一下δ13C是怎么定义的

语是怎么定义的?用什么做参照标准?
The δ13C value is defined as the C-13 isotope concentration in a sample
relative to that in the standards. The standard is a particular limestone
from the Pee Dee Belemnite fossil (PDB). The value can be expressed by this
equation:
δ13C = [(13C/12C)sample-(13C/12C)PDB]/[(13C/12C)PDB]
You can find more detailed definition, derivation and explanations in any
stable isotope textbook.
b*****r
发帖数: 203
46
来自主题: Pharmaceutical版 - 有 LC-MS 的工作么?
Since you are doing LC-MS/MS, why there is still a lot of matrix effect?
For LC-MS, you can quantitate it by their ion intensity. Of course, you need
control the spray condition similar or use an internal standard to
normalize the different electrospray condition. If you know what molecules
you are looking at, you can use an isotope labeled same molecule (heavy
isotopes such as C13 instead of D are better, since it won't have
significant effect on the retention time). In this case, you will have

发帖数: 1
47
橡树岭国家实验室散裂中子源诚招有物理和计算背景的博后。
课题为运用高性能计算增强非弹性散射中子谱仪的分辨率。年薪约$65k。
https://recruiting.ornl.gov/sap/bc/webdynpro/sap/zornl_hrrcf_c_post_doc?sap-
language=EN&sap-client=010#
Job Posting Title
Postdoctoral Research Associate in Super-Resolution Inelastic Neutron
Scattering
Purpose
The Neutron Sciences Directorate (NScD) at Oak Ridge National Laboratory (
ORNL) operates the High Flux Isotope Reactor (HFIR), the United States'
highest flux reactor based neutron source, and the Spallation Neutron Source
(... 阅读全帖
M*V
发帖数: 3205
48
来自主题: ChinaNews版 - PPT 来了
India plays key role in building super machine that will offer unlimited
energy
LONDON: India is developing the heaviest and the largest parts of the
Tokamak, the machine behind the biggest scientific collaboration on the
planet, to produce unlimited supplies of cheap, clean, safe and commercial
energy from atomic fusion.
The international nuclear fusion project known as ITER, meaning "the way" in
Latin, is based on the 'tokamak' concept of magnetic confinement, in which
the plasma is contained ... 阅读全帖
l*******G
发帖数: 1191
49
考,如果用钍同位素检验,应该很容易知道是否造假呀。因为60年代原子弹实验,之前
和之后的文物有大不相同的钍同位素比例。
这里有一个例子:http://www.bibleinterp.com/articles/alternative_interpretation.shtml
Uranium/Thorium dating: An additional method that could be potentially
used for age examination of the patina is the isotopic analysis of its
uranium and thorium content (Thompson 1973). The uranium-thorium to helium-
lead method has been previously used to date a small range of minerals,
particularly magnetite (hence relevant for the case in question). The m
Z**S
发帖数: 1211
50
For me, there is no solid evidence against anyone.
The basic fact is that 朱令离奇“铊”中, and it is not
clear which 铊 salt was used, and who did it.
Based on statistics from previous cases all over the world,
it is more likely a homocide (70%). However,
considering Zhu's sister died of unnatural causes at
similar age, suicide is a formal possibility (30%).
People of close contact to Zhu are suspects. These
include her roommates, boyfriend, social contacts
(i.e. players in the orchestra), even parents.... 阅读全帖
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