b******s
发帖数: 1089 | 1 很多诱导型启动子都有leaky的现象，我自己在实验室也发现了。但是leaky的情况不同
的株系都不完全相同。好像heat-shock或者ethonal,tet等leaky比较厉害，植物里很多
人用雌二醇似乎效果不错。但是我感觉有些启动子后面带雌二醇似乎效果很好，有些也
有明显的leaky现象。
想知道这些leaky是诱导组件的原因，还是前面的启动子太强，可以绕过后面的诱导组
件微弱诱导基因表达？
有没有改进的方法，推荐点文献最好，谢谢！ |
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s*****y
发帖数: 32 | 2 同问。我试了各种方法都没有解决leaky的问题。我的实验当中只要有一丁点leaky就有
表型了。 |
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g******r
发帖数: 512 | 3 加国的天寒地冻有关。1980年代政府为了要节省能源，便推出一些新的建筑规则，要盖房子就得盖不通风的房子，以免暖气外流。有专家指出，要在卑诗省盖不通风的房子行不通，因为此地雨水多，结果联邦也理不了这么多专家的意见，国家改了建筑规则，下面的城镇也跟着改。最后结果便是原来不通风的墙却会大量吸入湿气，而湿气是不会散的，因为是密不通风的墙。本省便发生了leaky condo的悲剧。
这是悲剧，因为一个人的房子往往是他一生最重要的财产，那些要退休或已退休的人士，如果住在一个漏水屋里，往往便要花上几万元到十几万元去修理，另外除了要受修理的不便，还要受到房子
就算修好后的跌价。对于这些完全无辜的人，他们的解药是什么？往往除了自认倒楣，便是一些没有太有效的官司。
告建筑商，告承建商，告市政府，告所有人…，告到后来，替他们告人的律师便会说，建筑商愿意赔你20%的损失，扣掉律师费之后，你们还可以取得12%的赔偿。如果不接受，房主每户要另外再给一万元律师费。就算打赢官司，建筑商也有可能破产或无钱赔。至于政府，律师会说政府是没得告，他们不会赔钱，所以搞到最后，这类官司中房主收回损失的10%到15%已算很好，25 |
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s******y
发帖数: 220 | 4 我们有个老鼠模型，应该是DOX诱导蛋白表达后老鼠慢慢生病，但是现在发现没诱导老
鼠就病了，应该是leak的问题吧。
在这样的情况下，有什么办法控制basal leakiness 吗？
而且母老鼠明显比公老鼠leak 更严重，这make sense 吗？
谢谢！ |
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a******8
发帖数: 56 | 5 现在你的data还不能证明有leakiness. 需要western blot或者northern确认。
多数inducible mouse是转基因的，通常需要从十几个strain中挑出最好的。
选择的标准有，表达量、表达的pattern等等。而且很重要的一点就是要有2个以上
strain有共同的表型，从而排除integration site 造成表型的误导。
你的实验才刚开始，作老鼠很费时间，花点时间鉴定好的strain是很值得的。否则今后
都是白做。祝好运。 |
|
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m***c
发帖数: 637 | 7 what is leaky junctions?
thanks! |
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f****s
发帖数: 126 | 8 经细胞间的『渗漏接合』(leaky junction) 排出到体外( |
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m***c
发帖数: 637 | 9 鰓的構造與富含粒線體細胞之觀察
吳郭魚的鰓，由鰓弓、鰓耙、鰓絲和鰓薄板所構成，每一條鰓弓上有著鰓耙和
成對的鰓絲，鰓絲上排列有成對的鰓薄板 (圖十)。鰓上的細胞由扁平細胞、黏液細胞
、未分化細胞及MR細胞 (圖二十一) 組成。其中MR細胞與滲透壓調節相關，也是鰓上主
要負責離子調節的細胞(陳金貝，1999)。
利用粒線體染色法，發現長期馴養在海水的吳郭魚，比長期馴養在淡水的吳郭
魚確實有較多的MR細胞，且粒線體的濃度也較高，用以排除過多的鹽份。廣鹽性魚類在
適應海水的過程中，MR細胞的作用機制是以位於管狀系統中的Na+,K+-ATPase為原動力
，維持胞外高鈉、胞內高鉀的狀態，胞外的鈉帶著氯經由頂端細胞膜上的載體進入細胞
內，而後胞內的負電位將氯離子再經頂端細胞膜直接排到外界環境中，鈉離子則經由底
側邊細胞膜上的Na+,K+-ATPase排到細胞外，經細胞間的『滲漏接合』(leaky junction
) 排出到體外 (圖二十二)。至於生活在淡水中的廣鹽性魚類，魚體則以Na+,K+-ATPase
為動力，主動吸收外界環境中的鈉離子。因此維持魚類體液中離 |
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f**d
发帖数: 768 | 10 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
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v**********6
发帖数: 86 | 11 已经gg,记得的一些题目分享给大家，同时也看看大家有什么解法，自己现在还不是很
确定:) 因为是一个architect的职位，所以不是纯软件的问题，有的可能需要一些
power的背景知识。
1. Suppose we have 2 pipelined hardware multipliers(or some other hardware).
One is working at 1GHz with 2 operations executed in parallel at the same
time. The other one is at 500GHz with 4 operations. Suppose we have
transistors of 3 types(low leaky(30%),middle leaky(50%) and high leaky(70%))
and here leaky means leakage power of the transistor. Which multiplier
should we use considering the 3 types ... 阅读全帖 |
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c****p
发帖数: 6474 | 12 1. Suppose we have 2 pipelined hardware multipliers(or some other hardware).
One is working at 1GHz with 2 operations executed in parallel at the same
time. The other one is at 500MHz with 4 operations. Suppose we have
transistors of 3 types(low leaky(30%),middle leaky(50%) and high leaky(70%))
and here leaky means leakage power of the transistor. Which multiplier
should we use considering the 3 types of transistor?
P_dynamic = alpha * f（^2？） * V * C
P_static = V * C * 1/f
第一个f是两倍，C是一倍。第二个f低，C大于... 阅读全帖 |
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j********o
发帖数: 2 | 13 Yes. Not only BL21codon plus, but almost all BL21s except BL21(DE3)pLysS/E are
leaky. pLysS/E encode enzyme that degrade T7 polymerase to avoid leaky.
In your case with many rare Arg codons, you have to use codon plus. So, it is
importment to avoid leaky? otherwise, just express with leaky if it does no
harm. |
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s*******n
发帖数: 452 | 14 What Goes Wrong With Cars, and When
By Larry Carley c2009
Owning and driving a car involves two things most motorists would rather
avoid: spending money on maintenance and spending money on repairs.
If you do not maintain your car (meaning regular oil changes, filter
replacements and keeping an eye on critical fluid levels), you will be faced
with major repairs much sooner than you should. Most people realize a car
is an investment, and to get the most out of your investment you have to
take car... 阅读全帖 |
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W***n
发帖数: 11530 | 15 FBI Files Say Hedge Fund Analyst Made Millions on Tips From Dell Insider
By Neil Weinberg, David Glovin and Patricia Hurtado Nov 25, 2014 4:00 AM CT
Manhattan U.S. Attorney Preet Bharara said Jan. 7, “Part of our job is to
make sure... Read More
The FBI files spell it out: An analyst at Citadel LLC, the hedge fund with $
23 billion in capital invested globally, told agents he made millions of
dollars trading on information from a company insider.
It was December 2011, and the Justice Department ... 阅读全帖 |
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m**a
发帖数: 296 | 16 如果阁下在电脑上打入leaky condo，你就会发现全世界都好像没有这个问题，只
有在卑诗省是leaky condo漏水屋的发源地。但最后要对漏水屋负责任的，往往是建筑
商，而非市政府设定建筑规则的人士。
查漏水屋和加国的天寒地冻有关。1980年代政府为了要节省能源，便推出一些新的
建筑规则，要盖房子就得盖不通风的房子，以免暖气外流。有专家指出，要在卑诗省盖
不通风的房子行不通，因为此地雨水多，结果联邦也理不了这么多专家的意见，国家改
了建筑规则，下面的城镇也跟着改。最后结果便是原来不通风的墙却会大量吸入湿气，
而湿气是不会散的，因为是密不通风的墙。本省便发生了leaky condo的悲剧。
这是悲剧，因为一个人的房子往往是他一生最重要的财产，那些要退休或已退休的
人士，如果住在一个漏水屋里，往往便要花上几万元到十几万元去修理，另外除了要受
修理的不便，还要受到房子
就算修好后的跌价。对于这些完全无辜的人，他们的解药是什么？往往除了自认倒楣，
便是一些没有太有效的官司。
告建筑商，告承建商，告市政府，告所有人…，告到后来，替他们告人的律师便会
说，建筑商愿意赔你20%的损失，扣掉律 |
|
s******d
发帖数: 105 | 17 如果阁下在电脑上打入leaky condo，你就会发现全世界都好像没有这个问题，只有在卑诗省是leaky condo漏水屋的发源地。但最后要对漏水屋负责任的，往往是建筑商，而非市政府设定建筑规则的人士。
查漏水屋和加国的天寒地冻有关。1980年代政府为了要节省能源，便推出一些新的建筑规则，要盖房子就得盖不通风的房子，以免暖气外流。有专家指出，要在卑诗省盖不通风的房子行不通，因为此地雨水多，结果联邦也理不了这么多专家的意见，国家改了建筑规则，下面的城镇也跟着改。最后结果便是原来不通风的墙却会大量吸入湿气，而湿气是不会散的，因为是密不通风的墙。本省便发生了leaky condo的悲剧。
这是悲剧，因为一个人的房子往往是他一生最重要的财产，那些要退休或已退休的人士，如果住在一个漏水屋里，往往便要花上几万元到十几万元去修理，另外除了要受修理的不便，还要受到房子就算修好后的跌价。对于这些完全无辜的人，他们的解药是什么？往往除了自认倒楣，便是一些没有太有效的官司。
告建筑商，告承建商，告市政府，告所有人…，告到后来，替他们告人的律师便会说，建筑商愿意赔你20%的损失，扣掉律师费之后，你们还 |
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m*******e
发帖数: 1598 | 18 BY TINA AMIRTHA8 MINUTE READ
This story contains interviews with Christian Kästner, assistant
professor at Carnegie Mellon University; Amy Hirotaka, state policy and
advocacy manager at Code.org; and Janet Siegmund, researcher at the
University of Passau.
In order to expose more students to computer science, 17 states have passed
legislation to create basic math and science requirements in the curriculum,
rather than count them as electives. But lawmakers in Texas have made
computer program... 阅读全帖 |
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x*****z
发帖数: 15 | 19 Leaky bucket 就是个带时间记录的counter，具体可以看guava ratelimiter
：leaky bucket用queue实现可以么？？ |
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x*****z
发帖数: 15 | 20 Leaky bucket 就是个带时间记录的counter，具体可以看guava ratelimiter
：leaky bucket用queue实现可以么？？ |
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j*******8
发帖数: 260 | 21 I am looking at a house built in 1900.
The first floor seems ok. The floor of second floor are seriously not level
.
The basement is granite and leaky. The wall that is leaky is saggy or
curved.
Are those very serious problems? The price is pretty good and builder redid
plumbing and wire and added new furnaces. Other than problems above, the
house seems in a great shape.
I am really struggling. I am not sure if the basement will become a big
headache later. What might cause the level probl... 阅读全帖 |
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J***n
发帖数: 210 | 22 You are right that a casual tests like CBC blood tests or common ECG may or
may not really reveal potential heart problems.
I just happen to read the following post and would like to share.
It is a long read but the gist is that this guy paid $1400 to have a really
thorough tests because he too find casual blood test is just not enough.
Lucky for him his heart is find although they find tiny scar tissue at his
lung and some minor issues with this spine. The same tests done for his
business partn... 阅读全帖 |
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l*****k
发帖数: 587 | 23 too long to read :)
I think it is possible pet20 have leaky expression, it may kill the cells.
in this case, I think novagen have a cell line that won't have leaky expression.
Instead of cloning disgested fragment, how about do blunt cloning using
novagen's perfect cloning kit? then cut it, at least you won't worry about the
short seq at the restriction site, after cloning,
with multiple clone sites on both side, you
have more choice to put it to pet20, though you have to worry about the problem |
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D*a
发帖数: 6830 | 24 不知道你说的partial KO是指什么，大多数时候用cre就是为了partial KO，这个
partial是specific的patial，比如只在特定器官，或者某种特定细胞中造成KO而不涉
及其他细胞，这样就避免了用total KO或者用全身+/-的老鼠，分析实验结果时不能确
定是整体影响，还是特定细胞的特定功能。
但有的partial KO是我们不希望的，比如利用脂肪细胞中的特定promotor建立了一个脂
肪细胞中表达的cre，然后每个器官分别摘出来验证时，发现在肝中也有KO，这就是cre
是leaky的，这个当初做老鼠时很难避免，因为你也不知道你的promotor是不是多用了
一块或者少用了一块，或者旁边一个调控元件根本就是未知的，再加上后面接的又是别
的蛋白，很难预测promotor的行为，比如到底在哪里表达，什么时候表达等等。这时候
paper发表前和发表后就很容易被人critique，不过也不是不能发到好杂志上，这个原
因就不分析了哈。。。但是结果其实有可能是leaky的那部分cre造成的，而跟你本来想
KO的那个器官没有关系。。。
我现在看的一篇nature文章，我 |
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s******r
发帖数: 2876 | 25 哪一篇？说来学习学习。
不知道你说的partial KO是指什么，大多数时候用cre就是为了partial KO，这个
partial是specific的patial，比如只在特定器官，或者某种特定细胞中造成KO而不涉
及其他细胞，这样就避免了用total KO或者用全身+/-的老鼠，分析实验结果时不能确
定是整体影响，还是特定细胞的特定功能。
但有的partial KO是我们不希望的，比如利用脂肪细胞中的特定promotor建立了一个脂
肪细胞中表达的cre，然后每个器官分别摘出来验证时，发现在肝中也有KO，这就是cre
是leaky的，这个当初做老鼠时很难避免，因为你也不知道你的promotor是不是多用了
一块或者少用了一块，或者旁边一个调控元件根本就是未知的，再加上后面接的又是别
的蛋白，很难预测promotor的行为，比如到底在哪里表达，什么时候表达等等。这时候
paper发表前和发表后就很容易被人critique，不过也不是不能发到好杂志上，这个原
因就不分析了哈。。。但是结果其实有可能是leaky的那部分cre造成的，而跟你本来想
KO的那个器官没有关系。。。
我现在看 |
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l**********1
发帖数: 5204 | 26 Did you try test the level of leakiness with no isotope label actin?
i mean whether your target even abundantly but still holded entirely intra- cellular tyrosine-
containing protein, such as actin or tubulin:
then your negative control with 35S labeled that also became Immunoprecipitation positively
Details please go to
Protocol full text link:
//webdoc.nyumc.org/nyumc/files/sun-lab/attachments/CPCB.ch07.Protein%
20Labeling.pdf
cited :
>For many applications, an important control for surface-l... 阅读全帖 |
|
l**********1
发帖数: 5204 | 27 Did you try test the level of leakiness with no isotope label actin?
i mean whether your target even abundantly but still holded entirely intra- cellular tyrosine-
containing protein, such as actin or tubulin:
then your negative control with 35S labeled that also became Immunoprecipitation positively
Details please go to
Protocol full text link:
//webdoc.nyumc.org/nyumc/files/sun-lab/attachments/CPCB.ch07.Protein%
20Labeling.pdf
cited :
>For many applications, an important control for surface-l... 阅读全帖 |
|
c********r
发帖数: 1125 | 28 不是这个领域的，但是读过一些这个领域的文章，另外主要的3个实验室boss的seminar
听过2个，瞎说几句，真正内行不要笑话。。。
实际从前两年开始已经有开始质疑lgr5+cell 是isc细胞的了，比如这篇文章。
Nature. 2011 Sep 18;478(7368):255-9.
A reserve stem cell population in small intestine renders Lgr5-positive
cells dispensable.
这篇ncb是hans clevers实验室也就是最早发现lgr5并且建立整个story体系的实验室，
以前他们还做出了非常fancy的lgr5＋细胞的单细胞体外培养出有功能的器官（小肠，
胃。。。）
但是越来越多的证据显然显示lgr5不是唯一的决定性因子，这篇cell的主要卖点觉得就
是这个，所以lrig1＋与lgr5＋的细胞到底是不是一群实际上是非常关键的问题。。。
如果cell的data是确实的话那么lgr5的重要性显然要大打折扣，但也不至于完全颠覆，
毕竟clevers实验室的体外单细胞培养器官再造和动物移植模型已... 阅读全帖 |
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t*d
发帖数: 1290 | 29 IMHO, to find novel drug delivering methods might be better ways to conquer
cancer comparing to studying the mechanisms of cancer initiation and
progression.
Nature 491, S58–S60 (22 November 2012)
Nanotechnology: Carrying drugs
When Joseph DeSimone makes nanomedicines, he compares himself to a baker. He
mixes drugs with different chemical 'batters', puts them in tiny moulds,
cures them and then turns them out. He can mould almost any shape: discs,
cubes, long sticks, roughened doughn... 阅读全帖 |
|
l**********1
发帖数: 5204 | 30 RE LZ
pls refer
PMID 21063464
Liu Y et al.
Tamoxifen-independent recombination in the RIP-CreER mouse. (2010).
PLoS One. 25: e13533.
http://www.ncbi.nlm.nih.gov/pubmed/21063464
>There are at least two possible explanations for
>these discrepancies.
>One possibility is that after many generations
of backcrossing to the C57BL/6 background, the expression of the
randomly integrated RIP-CreER transgene is enhanced, thus
increasing the probability of its nuclear entry, even in tamoxifenuntreated
cel... 阅读全帖 |
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z****u
发帖数: 1007 | 31 leaky 是组织细胞腐败降解了。 跟冻融没关系。单次的组织冻融不影响，比如-20度保
存很久的老鼠解冻后再固定也没啥问题。 leaky一旦发生就没法解救了，sample只有
扔掉。所以我的sample都是杀死后立马扔PFA. 一般来说GFP或者TdTomato 都不会被PFA
灭掉。 实在灭掉了那就上Ab . 很多公司都有很好的GFP antibody.很特异，很强。
组织一旦固定了就不用担心leakage 了。 |
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s*****n
发帖数: 40 | 32
I know only a little about this.
You can ask lyzh or maybe jessejane.
Leaky bucket is kind of traffic shaping algorithm.
Regular traffic can have arbitary pattern that is unknown,
as we have mentioned before. However, traffic shaper
confines the traffic and make the output traffic conform to
certain pattern. (Usually confines the average rate and burst rate)
Leaky bucket is the most popular implementation of
shaper and is proposed in the IETF InterServ and DiffServ.
You can find intro in comput |
|
t*d
发帖数: 1290 | 33 【 以下文字转载自 Biology 讨论区 】
发信人: ttd (oldcat), 信区: Biology
标 题: better way to conquer cancer
发信站: BBS 未名空间站 (Wed Jan 16 14:57:14 2013, 美东)
IMHO, to find novel drug delivering methods might be better ways to conquer
cancer comparing to studying the mechanisms of cancer initiation and
progression.
Nature 491, S58–S60 (22 November 2012)
Nanotechnology: Carrying drugs
When Joseph DeSimone makes nanomedicines, he compares himself to a baker. He
mixes drugs with different chemical 'batters', ... 阅读全帖 |
|
a********e
发帖数: 3771 | 34 02' 9-3 SE. 75k when I bought it. Now it is 88+K.
My car was factory-certified in 2005 before the second owner bought it. When
I got the car, there were a few defects.
1. a missed splash shield on the driver side.
2. the actuator (motor) of trunk lock did not work. I took it off the car
and manually lock the trunk by key.
3. headlamp blades on the driver side did not work well.
4. a loose parking brake.
5. a broken antenna post.
6. a broken fog lamp cover. The bulbs of both fog lamps died.
Excep... 阅读全帖 |
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e********3
发帖数: 18578 | 35 Diodorus gives an account of Xanthippus' death. After the battle of Tunis,
Xanthippus stopped in the city of Lilybaeum which was besieged by the Romans
. He inspired courage and led an attack defeating the Romans. Jealous of
Xanthippus' success, the city betrayed him by giving him a leaky ship and he
supposedly sank in the Adriatic Sea on his voyage home.
Diodorus Siculus (Greek: Διόδωρος Σικελιώτη&#
962;), was a Greek historian who lived in the 1st century BC. According to
Diodor... 阅读全帖 |
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s******n
发帖数: 518 | 36 既然你关心农药，那就推荐一个孟山都的roundup的危害的文章：
https://www.ecowatch.com/15-health-problems-linked-to-monsantos-roundup-
1882002128.html
Food
Guest Contributor
Jan. 23, 2015 11:44AM EST
15 Health Problems Linked to Monsanto's Roundup
Monsanto invented the herbicide glyphosate and brought it to market under
the trade name Roundup in 1974, after DDT was banned. But it wasn’t until
the late 1990s that the use of Roundup surged, thanks to Monsanto's
ingenious marketing strategy. The strategy? Genetically engineer ... 阅读全帖 |
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m**********n
发帖数: 27535 | 37 Didn't have anything but a leaky tent when I was in 'Nam, shared it with ten
others when I was lucky enough to be at the base camp...no emails,
computers, etc.. just Agent Orange and the bad guys...
Saturday, June 25, 2011 7:28:16 PM |
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m**********2
发帖数: 6568 | 38 用胶带来修泄露的核废料罐，来节约经费。
我开始好奇为什么日车便宜了。连人命关天的核电站都能这么省钱，还有什么是不能省
的？
‘Duct tape, wire nets’ were used to mend Fukushima water tanks - worker
http://rt.com/news/fukushima-radiation-water-leak-186/
As TEPCO began preparations for the cleaning of the drainage system with
tons of leaked radioactive water at the Fukushima power plant,a former
employee reveals the reason for so many leaks was cost cutting measures such
as using duct tape,Asahi reported.
Yoshitatsu Uechi, auto mechanic and tour-bus driver, wo... 阅读全帖 |
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m****8
发帖数: 327 | 39 如果不是很小就有的问题，那一定是什么触发的，滥用抗生素？
可以检查下是不是有leaky gut和candida overgrowth。可以试下吃zinc和raw garlic
。同时考虑灌肠或者自己做enema。看看效果如何。 |
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a******4
发帖数: 209 | 40 You know something.
Sadly I am taking almost all what you mentioned except enema, only slight
improvement.
To treat possible leaky gut or candida overgrowth, I am also taking a lot
supplements like L-glu, grapeseeds, garlic, some improvement, but still a
problem when I work in the office.
Right now the only thing work for me is antibiotics, I took one yesterday
and my odor decreased a lot today. However it only work as long as I am
taking it, not for long term since bacteria will gain resistanc... 阅读全帖 |
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z****9
发帖数: 2484 | 41 有专门的内裤，过滤臭气的吧
You know something.
Sadly I am taking almost all what you mentioned except enema, only slight
improvement.
To treat possible leaky gut or candida overgrowth, I am also taking a lot
supplements like L-glu, grapeseeds, garlic, some improvement, but still a
problem when I work in the office.
Right now the only thing work for me is antibiotics, I took one yesterday
and my odor decreased a lot today. However it only work as long as I am
taking it, not for long term since bacteria wi... 阅读全帖 |
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b********n
发帖数: 38600 | 42 China will do: BLOCK THE US VESSELS.
Along with the PLA Navy, China will float every leaky, rusty junk it can get
it's hands on. And intermingle them with their state-of-the-art vessels.
So all you General Jack D. Rippers out there (yes, you) put it back in your
pants and save your precious bodily fluids for the missus.
No ship in the US Navy is going to risk damage to its bow 6000 miles from
home port in unfriendly waters by ramming a dilapidated, 300 foot long hot
water heater. |
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b********n
发帖数: 38600 | 43 “If I were dictator, I would send them all back to Africa on a leaky boat.
Problem solved.”
Posted 17 hours ago |
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c*********d
发帖数: 9770 | 44 送交者: Pascal 2016年03月27日18:36:30 于 [天下论坛]
“200,000years ago we were all african blacks”的图片搜索结果
Chinese Scientist Proves The First Inhabitants Of China Were Black！
Updated on October 9, 2014
Black Chinese
Kongo (Negro) - Chilamache-Chinese Skulls 1855
Out Of Africa 10/21/2012
For many years Black historians and Afrocentrists have said that the first
inhabitants of China were black Africans.
"The Negroid races peopled at some time all the South of India, Indo-China
and China. The South of Indo-Chin... 阅读全帖 |
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x****6
发帖数: 4339 | 45 湖南：屌屌，屌脐根
广东白话：「屌」（𨶙）、「鳩」（𨳊、尻、九）「撚」
英语：
Adolph
Albino Cave Dweller
baby-arm
baby-maker
baloney pony
beaver basher
beef whistle
bell on a pole
bishop
Bob Dole
boomstick
braciole
bratwurst
burrito
candle
choad
chopper
chub
chubby
cock
cranny axe
cum gun
custard launcher
dagger
deep-V diver
dick
dickie
ding dong mcdork
dink
dipstick
disco stick
dog head
dong
donger
dork
dragon
drum stick
dude piston
Easy Rider
eggroll
Excalibur
fang
ferret
fire hose
flesh flute
flesh tower
froto
fuc... 阅读全帖 |
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t**x
发帖数: 20965 | 46 Chet Seligman Point Reyes Station, Ca 1 hour ago
Why do so many Chinese study physics and math relative to Americans?
The answer may be similar to why Germans, Swiss and Danes studied physics
and math, relative to Americans, prior to WWII.
That being, a long history of intellectual cultures in those other countries
and the lack of one here.
It is hard to be a cowboy and a physicist simultaneously.
Reply 1Recommend
John Mardinly Chandler, AZ 1 hour ago
Hi-tech companies like Intel could not f... 阅读全帖 |
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g********2
发帖数: 6571 | 47 brnfream 25 minutes ago
I wonder how willing the European Countries and the UK will be to share
intelligence information with leaky hillary/billary if they were POTUS. I
sure wouldn't be.
quickrunitspelosi 26 minutes ago
Tomorrow the democrats will try to raise money off the attack in France.
They will tell Distillery supports that in order for us to avoid this same
kind of attack here, we will need to donate to her slush fund, oops I mean "
Foundation" and her campaign. And she will promise to ... 阅读全帖 |
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b*****d
发帖数: 61690 | 48 http://thehill.com/homenews/administration/318621-trump-white-house-besieged-by-leaks
Donald Trump’s White House has sprung a remarkable number of leaks since
his inauguration.
The question is where they’re coming from.
Barely a day goes by without some unusual tale emerging from 1600
Pennsylvania Ave. Recent examples in a single New York Times story included
the assertion that White House aides have held meetings in the dark because
they can’t figure out how the lights work and that the preside... 阅读全帖 |
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T**********e
发帖数: 29576 | 49 Sources: White House Leaks at Center of Discussions for Katie Walsh
Replacement
Multiple senior White House sources tell Breitbart News that discussions to
replace now former Deputy Chief of Staff Katie Walsh—an ally of Chief of
Staff Reince Priebus who was removed from her position on Thursday—center
on who has been leaking information out of the White House.
Walsh was suspected of being a leaker, White House sources tell Breitbart
News. They specifically say that a story first reported on Char... 阅读全帖 |
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