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全部话题 - 话题: melting
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e**********2
发帖数: 310
1
来自主题: Fashion版 - 说说昨天刚败的lush
更新一下lush使用的感受。从发这个贴开始我就一直在用,头发状况良好,松软不毛躁
,最近很热,但在户外走一下午头皮也不会觉得痒,而且皂体放在卫生间,那叫一个沁
人心脾啊,大赞~
说两点不好的:
一,不推荐sale让我买的solid conditioner,虽然效果还不错,但是难推开,并且有
股中草药铺的味道,很难闻。
二,这个皂体比它家圆形的那种软,所以容易melt,我每天洗头,现在应该刚好过去半
个月,也就是15天,皂体只剩下一半了,melt很多,从皂托底部漏掉了,很是心疼,不
知道是我方法不对,还是这个皂本身如此?请达人指教。
A*******d
发帖数: 1799
2
又是一年四月来,春已深,园已绿,咱就捡几种好看的show一下,混个包子吃,哈哈。
首先展示一下豌豆苗,因为俺家LD最喜欢吃。今年俺试着肿了四种,做了一下比较,下
面介绍一下实验结果,让各位菜农朋友们作为参考.
下图是四种豌豆的种子图:
(1)Melting Sugar (2)ALASKA PEA (3) SNAP PEA (4) Gray Sugar
下面这是ALASKA 豌豆苗,个头大家看着感觉如何?叶子大的和俺家宝宝的小手一样大
,嫩芽尖很美味的,俺这对豌豆苗一般的人,也很喜欢。记得班上有人说种不出商店里
卖的那么大的豌豆苗,事实说明这豌豆苗比商店里卖的苗大而嫩。
其余的豌豆苗,味道也还行,就是个头稍微有点小,悲剧的是今年三月温度创历史最高
,俺这5区的农民眼睁睁看着 Melting Sugar 没来得及吃它的苗,嘿,开花结果了,不
过不算太悲剧,家里宝宝很爱吃生的豌豆荚,算是意外的收获。下图是他的开花图。
结论就是大家要是收获豌豆苗来吃的话,建议种ALASKA Pea比较好。如果是收获逗的话
,俺就不知道了。
顺便show一下俺的箭在弦上的蒜苔,再等两个星期就应该可以收获蒜苔... 阅读全帖
g***e
发帖数: 14217
3
枇杷的品种介绍:http://www.crfg.org/pubs/ff/loquat.html
Orange-fleshed Varieties
Big Jim
Originated in San Diego, Calif. by Jim Neitzel. Large, roundish to
oblong fruit, 1-1/4 to 1-1/2 inches in diameter. Skin pale orange-yellow,
medium-thick, easy to peel. Flesh orange-yellow, very sweet but with some
acidity, of excellent flavor. Ripens midseason, March to April. Tree
vigorous, upright, highly productive.
Early Red
Originated by C. P. Taft in 1909. Medium-large, pear-shaped fruit, borne
in com... 阅读全帖
y****2
发帖数: 11909
4
来自主题: gardening版 - 菠萝包
记得上小学的时候俺妈去广东探亲,回来时带了几个菠萝,第一次闻到这么香的菠萝,
当时眼睛就睁大了,口水也流出来了。这么多年过去了,好象再也没有闻到过那熟悉的
香味了,最近在一本书上看到菠萝包三个字,还有那详细的做法,手不禁开始痒痒了。
好吧!不管成功与失败,总得先去尝试一下对吧!好在运气不错,没有特别失败,也就
算小小的成功了一把。宝贝闺女特爱吃,今天最后一个,边吃还边提醒俺:"妈妈,最
后一个菠萝包我吃了哈?"
做法复杂,强力不推荐,前后花去三小时。很悲催的是,这个方子里用的是罐头装的菠
萝,而且还只需要一勺,真不过瘾😄
发酵小面团原料:
1/4 ounce 干发酵粉
3/4 teaspoon 糖
1/2 cup 温水
3/4 cup bread flour
面团原料:
4 cups bread flour
3/4 cup 再加上2 tablespoons 糖
3 eggs
1/2 cup 温水 (切记:不要一次性加入,慢慢加入,后有说明)
1/4 cup 牛奶
2 tablespoons 干奶粉(dry milk)
1/4 cup melted butter
topp... 阅读全帖
a*********7
发帖数: 30080
5
来自主题: LeisureTime版 - 以后不能再练铁头功了
Melting ice could push penguins to extinction
http://www.newscientist.com/article/dn16487-melting-ice-could-p
你以后少吃点冰吧,那是企鹅宝宝的床哩
j******n
发帖数: 21641
6
来自主题: LeisureTime版 - Uri Geller的‘魔术’
今天江苏卫视跨年晚会请了颇具争议的UG
我们在电视机前试了他的调羹弯曲实验
居然成功了
做法是拿家里一个spoon
在他让你用手摩擦并且口中念念有词melt melt/溶化溶化时
我们的spoon果然弯了
他声称他有外星人给的魔力
江苏现场很多人的调羹都弯了,我远隔重洋也弯了
j******n
发帖数: 21641
7
来自主题: LeisureTime版 - 麦当劳广告为什么那么诱人
Ketchup, mustard and condiments are applied with surgical precision via a
syringe to the edge of the burger.
The cheese is gently melted using a blowtorch and heated palette knife to
achieve perfect "melted-ness".
Ingredients such as pickles and onions are moved to the front of the burger,
to avoid being concealed in the middle.
The bun is smaller than appears in the ad as it contracts due to "steam in
the box".
Small inconsistencies such as stray sesame seeds, blemished and crumbs are
photo-sho... 阅读全帖
a********a
发帖数: 3176
8
来自主题: LeisureTime版 - Shel Silverstein的儿童诗
(刚发给K12俱乐部给孩子爸妈看。这里有孩的人家也爱读他的诗吗?他给花花公子画
过漫画, 我还没见过。)
我第一次听说他的诗,还是大孩子才1,2岁的时候,一天电视节目'NOGGING'读了
首他的"Ickle Me, Pickle Me, Tickle Me Too", 一个男声慢慢地读, 语调很幽默,
那些字在一起听起来格外上口,好玩, 讲的内容又有点怪异荒诞,我很喜欢:
Ickle Me, Pickle Me, Tickle Me too
Went for a ride in a flying shoe.
"Hooray!"
"What fun!"
"It's time we flew!"
Said Ickle Me, Pickle Me, Tickle Me too.
Ickle was captain, and Pickle was crew
And Tickle served coffee and mulligan stew
As higher
And higher
And higher they flew,
Ickle Me, Pickle Me, Tickle Me to... 阅读全帖
h**********s
发帖数: 171
9
来自主题: LeisureTime版 - 歌词赏析
She Said
专辑:
Scratch Acid
歌手:
Scratch Acid
来源:
Scratch Acid
And she said:
She said...
So you can play with me if you want to."
AAAAGGGHHh!!
Oh, not so much lethargic
As so much uninterested,
So I kept entering into her
Interesting intercourse of events
Uuugghh I noticed her face was melting
But if you put yourself in my shoes!
What would I,
Say?
Yeah uh, we got a missing person, and we gotta STD, and we gotta know, so..
"I'm not using my body now,
"I'm not using my body now, so you can play with... 阅读全帖
wh
发帖数: 141625
10
来自主题: LeisureTime版 - Love After Love (转载)
【 以下文字转载自 Poetry 讨论区 】
发信人: Lihebo (河伯见海若,傲然夸秋水), 信区: Poetry
标 题: Love After Love
发信站: BBS 未名空间站 (Thu Apr 30 09:44:03 2015, 美东)
Love After Love
Walking down this road,
afraid my loneliness will multiply,
I talk to my own shadow.
Waiting, at the end of the road,
is a love that is reborn
from the ashes of a volcano.
It has been too long between two eruptions.
Besides my shadow, I had no other companions.
The moon saw this love come twice
when she unbuttoned her black bodice,
once in the sky... 阅读全帖
L****o
发帖数: 1642
11
来自主题: LeisureTime版 - 【诗】A Wild Goose Chase
A Wild Goose Chase
On the southern bank of Lake Erie,
I was an apparent stranger among locals.
I was not a treasure hunter, rather a wild goose chaser,
even though these two might have the same fate.
I was not disappointed though.
A fleet of wild geese flying afar
melted into the sunset, just the scene
I have dreamt of over and over again.
The air vibrated with my heart beats, which in turn
rhymed with the flutters of the geese's wings.
Above the water, a kite flew high.
A gust bent ... 阅读全帖
wh
发帖数: 141625
12
来自主题: LeisureTime版 - 【诗】A Wild Goose Chase
嗯。如果去掉it,那就是指melt into the moment。如果后面的从句是个完整句,好像
加个when (that might be the moment when i myself melt into it)读起来更顺。
大概when也可以省略,等auo来显摆他的英语知识,哈哈。
wh
发帖数: 141625
13
来自主题: LeisureTime版 - 拉风的纽约街头滑雪
http://www.youtube.com/watch?v=qRv7G7WpOoU
这雪滑得好爽……背景音乐是Frank Sinatra的“New York, New York”,爵士味十足
。歌词也潇洒:
New York, New York
I want to wake up in that city
That never sleeps
And find I'm a number one
Top of the list
Head of the heap
King of the hill!
附个完整歌词:
New York, New York
Frank Sinatra
Start spreading the news
I am leaving today
I want to be a part of it
New York, New York
These vagabond shoes
They are longing to stray
Right through the very heart of it
New York, New York
I want to wake up i... 阅读全帖
H********g
发帖数: 43926
14
来自主题: Joke版 - 无聊小发明!
11原来是这么回事啊
http://www.coolest-gadgets.com/20111017/ice-speed-chess-set/
We’re talking about the 11.99 Ice Speed Chess Set here, where the entire
idea is to win the game before the pieces melt. You can imagine what a
potentially messy game this could end up, so hopefully someone can come up
with a decent waterproof chess board that has drainage at the sides, where
the board itself is not entirely level so that the melted pieces can have
their water run off to a pail somewhere. Each purchase comes... 阅读全帖
R******d
发帖数: 5739
15
来自主题: Joke版 - 这就是变暖教的科研水平
我不是什么大牛, 这个也不是我专业。 但我相信要真的去较真, 你必须要有一定的
科学基础和专业知识。 这里大部分人可能没有专业知识, 质疑是对的, 但较真的话
水平可能不太够
quote
-------
II. The Vostok Ice-Core
To demonstrate the methods used in dating ice-cores I will use the Vostok
ice-core as an example because I found plenty of literature on it and
because it is an Antarctic ice-core which was what the original post was
about.
How It Was Collected
The Vostok Ice-Core was collected in East Antarctica by the Russian
Antarctic expedition. The Vostok Ice-Core is 2,083 meters lo... 阅读全帖
R******d
发帖数: 5739
16
来自主题: Joke版 - 这就是变暖教的科研水平
我不是什么大牛, 这个也不是我专业。 但我相信要真的去较真, 你必须要有一定的
科学基础和专业知识。 这里大部分人可能没有专业知识, 质疑是对的, 但较真的话
水平可能不太够
quote
-------
II. The Vostok Ice-Core
To demonstrate the methods used in dating ice-cores I will use the Vostok
ice-core as an example because I found plenty of literature on it and
because it is an Antarctic ice-core which was what the original post was
about.
How It Was Collected
The Vostok Ice-Core was collected in East Antarctica by the Russian
Antarctic expedition. The Vostok Ice-Core is 2,083 meters lo... 阅读全帖
H********g
发帖数: 43926
17
来自主题: Joke版 - 氮气派上用场了
这个说法错误,氩其实比氮气容易液化。因此可以用容易得到的液氮来液化或者固化氩
气,然后扔到被害人睡觉的屋子里去。

Melting point 63.15 K (−210.00 °C, −346.00 °F)
Boiling point 77.355 K (−195.795 °C, −320.431 °F)

Melting point 83.81 K (−189.34 °C, −308.81 °F)
Boiling point 87.302 K (−185.848 °C, −302.526 °F)
U*******g
发帖数: 261
18
【 以下文字转载自 PennySaver 讨论区 】
发信人: weever (单片机8098), 信区: PennySaver
标 题: 亚麻也够贼的,卖的便宜不干了,cancel order了
发信站: BBS 未名空间站 (Tue May 12 13:18:23 2015, 美东)
4块多钱还要贴运费12bottle的运动饮料,每个月subscribe,就4月寄了一次,5月告诉
我due to the weather怕melt不给寄了,5月这里才50度,饮料密封怕啥melt啊,又是
从本地仓库寄的,整个州气候还在春天呢。
丫小气觉得亏本了,找个理由不给了,也罢也罢,我也不是买不起
c*******o
发帖数: 8869
19
【 以下文字转载自 USANews 讨论区 】
发信人: gemini2012 (双子AB), 信区: USANews
标 题: 以史为鉴,diversity导致chaos
发信站: BBS 未名空间站 (Thu Aug 25 10:01:40 2016, 美东)
Diversity: History's Pathway to Chaos
Victor Davis Hanson
Posted: Aug 25, 2016 12:01 AM
Emphasizing diversity has been the pitfall, not the strength, of nations
throughout history.
The Roman Empire worked as long as Iberians, Greeks, Jews, Gauls and myriad
other African, Asian and European communities spoke Latin, cherished habeas
corpus and saw being Roman as pre... 阅读全帖
h*********e
发帖数: 6936
20
来自主题: LoveNLust版 - 幻世录(九)——特别篇
The one in the Terminater Movie...
Even the melting pot cannot melt...
Oh Yeah!!
L****o
发帖数: 1642
21
来自主题: Poetry版 - Love After Love
Love After Love
Walking down this road,
afraid my loneliness will multiply,
I talk to my own shadow.
Waiting, at the end of the road,
is a love that is reborn
from the ashes of a volcano.
It has been too long between two eruptions.
Besides my shadow, I had no other companions.
The moon saw this love come twice
when she unbuttoned her black bodice,
once in the sky, once in the water,
once in silence, once in music,
once real, once surreal. So many times,
I invited her to drink solitude at ... 阅读全帖
L****o
发帖数: 1642
22
来自主题: Poetry版 - 【诗】A Wild Goose Chase (转载)
【 以下文字转载自 LeisureTime 讨论区 】
发信人: Lihebo (河伯见海若,傲然夸秋水), 信区: LeisureTime
标 题: 【诗】A Wild Goose Chase
发信站: BBS 未名空间站 (Sat May 9 16:48:04 2015, 美东)
A Wild Goose Chase
On the southern bank of Lake Erie,
I was an apparent stranger among locals.
I was not a treasure hunter, rather a wild goose chaser,
even though these two might have the same fate.
I was not disappointed though.
A fleet of wild geese flying afar
melted into the sunset, just the scene
I have dreamt of over and over again.
T... 阅读全帖
J*******g
发帖数: 8775
23
来自主题: TrustInJesus版 - 跟基徒们一起查圣经.
Leviticus 18中说到迦南人各种乱伦,与邻居发生性行为(对婚姻的不忠),搞同性性
行为,与动物发生性行为,把孩子作为祭品,祭献给迦南人崇拜的偶像神Molek(不是
上帝)。
Deuteronomy 18这里还列出了迦南人其他的一切罪恶的活动,巫术,烧子女,等。
9 When you enter the land the LORD your God is giving you, do not learn to
imitate the detestable ways of the nations there. 10 Let no one be found
among you who sacrifice their son or daughter in the fire, who practices
divination or sorcery, interprets omens, engages in witchcraft, 11 or casts
spells, or who is a medium or spiritist or who consults the dead.
上帝... 阅读全帖
a**u
发帖数: 8107
24
☆─────────────────────────────────────☆
runsun (runsun) 于 (Fri Dec 18 14:14:14 2009, 北京) 提到:
美国加州2009.8.29
D:科学家说数十亿年前金星曾像地球有海洋但干枯了,部分原因是金星太接近太阳,它
有水存在可能表示那里曾有物质生命,今日科学家相信,金星有微生物存在,金星是否
曾像地球能长养生命?
M:是,它过去是,它曾是美丽的星球,现在已不再是,那里完全没有生命了,没有任何
生物,很遗憾。
D:那里过去曾有生命,他们看起来像我们吗?
M:是,是,他们看起来像我们。
D:火星,地球和金星为何都有类似人类的众生居住,我们有类似的祖先吗?谢谢M。
M:我们只有一个祖先那就是上帝,有些变成堕落天使,他们看起来类似我们,是因为我
们有类似的功德,包括我们银河中的火星,金星等星球,只问我们银河中的不要问太远
的星球,否则我就不用出定了。虽然你的问题很简单,但我要查证,需要一些时间,为
了地球的生存我们也都很忙,所以我得牺牲你们的要求,一直拖到今天,我好不容易才
排进今天的行程里。
D:谢谢M
... 阅读全帖
d*****r
发帖数: 5934
25
来自主题: AC版 - 请问:…谢谢!
多谢诸位好心人:
请问,有什么比较好的加工工艺来加工聚乙烯纤维的?
目前有melt spinning和melt extrusion,还有什么方法么?
其主要目的是增强复合材料的强度。
或者提供一些相关的网站也好,
再次谢过了!
s******u
发帖数: 10
26
清华92级化工系校友王鹏本周一不幸在上班的路上遭遇致命车祸身亡。Dr. Wang 2010 年9月开始在University of Rhode Island任职助理教授,2004年毕业于Columbia University. 期间曾经在Intel, 3M 和New Jersey Institute of Technology 任职。可以通过以下链接看到他的背景:
http://www.linkedin.com/profile/view?id=30215448&locale=en_US&t
http://www.egr.uri.edu/che/Faculty/pengwang.shtml
他在去往URI 的路上遭遇车祸。他的家住在21 Temple St., Hartford, Connecticut。他妻子叫Ran Luo, 也是清华校友,经管学院的。他有个7个月的女儿 Carolyn. 车祸的责任还在调查中。 车祸的照片,现场的录像和新闻可参考以下链接:
http://www.wpri.com/dpp/news/local_news/south_county/south-king
... 阅读全帖
c**l
发帖数: 63
27
来自主题: Fujian版 - Cappuccino
小资文字的重要道具之一,呵呵, 不过确实好喝
Cappuccino
by Gordon
One can never have too much coffee, as far as I’m concerned. This recipe
calls for grated coffee beans, which I’ve always thought absurd…use a very
fine ground espresso bean (turkish coffee ground size, if your grinder can
handle it). Also, don’t use a broiler unless you have a very good gas one,
such as those which are found in Amana ranges. A bad broiler won’t melt/burn
the sugar fast enough, and the custard will melt. If you don’t have a good
broile
k*******9
发帖数: 3361
28
来自主题: Shaanxi版 - 今天做了flourless brownies
但是被瓜分完了,木有照片,样子就和一般的brownie一样。上星期吃了我同事做的,觉得这个难得没那么甜,符合我们的口味,而且是用豆子做的,相对健康一些,于是要来了方子。方法很简单,连我都能成功做出来,大家也可以。方子如
下:
15 ounce can no-salt-added black beans, drained and rinsed
3 large eggs
1/3 cup melted butter(more for the baking dish)
1/4 cup cocoa powder
1/3 teaspoon salt
2 teaspoons gluten-free vanilla extract
1/2 cup plus 2 tablespoons cane sugar
1/2 cup gluten-free semisweet chocolate chips
1/3 cup finely chopped walnuts
Preheat oven to 350 degrees
Butter an 8-inch baking pan
Place the beans, ... 阅读全帖
x***m
发帖数: 184
29
My Magsafe only charges my computer intermittently and became very hot when
it is charging after 2 years of gentle and rare use as I use my iMac mostly.
It seems a common problem and may melt and cause burning based on the
review from other users. Just google "magsafe" and you will find a lot of
people complaining about the melt of the wire and burning with actual
pictures.
http://arstechnica.com/apple/news/2006/03/3229.ars
http://www.macuser.com/hardware/magsafe_melt_down.php
http://www.appled
j**********t
发帖数: 314
30
你说potential melt就potential melt了啊? 我看你是过了保修期想找个借口让
Apple给你免费换了。 真是猥
琐。
h****6
发帖数: 229
31
If it is high AT DNA, DNA may melt during gel running. If DNA melts, I
doubt it will re-form dsDNA under the same condition. ssDNA migrate slower.
s***e
发帖数: 911
32
如果是环, 100 bp的DNA在这么高的曲率下, 可能会有局部melting (one or a few bp)
来环解弯曲弹性能.在跑胶的温度下, 这样的local melting sites可能会有数个. 不同
的个数会导致DNA不同的构型, gel shift速度会不一样.
P******m
发帖数: 21
33
来自主题: Biology版 - real-time PCR问题
标准品是DNA,melting curve很正常,样品是RNA用random primer 做RT 来的cDNA,
melting curve有个小峰在主峰前面。样品RNA相对单一,没有non-target RNA 干扰。
貌似不是PCR的问题,是不是RT的问题,random primer做RT 造成的背景?qRT—PCR我
是新手,请人指点一下。
C*******e
发帖数: 4348
34
来自主题: Biology版 - real-time PCR问题
对,建议lz跑胶
melting curve是辅助
当你对melting curve有疑问的时候,那个样品还是要跑个胶才好
j*****9
发帖数: 716
35
加了melting curve步骤了没有?看每个孔的melting curve,产物是否单一,或者把产
物跑胶检样,看条带是否单一,引物有没有二聚体等等
m*********D
发帖数: 1727
36
(ZT)
Salt concentration
The phosphate groups are moderately strong acids, and thus they are always
ionized at physiological pHs. The two oxygens are equivalent, so they share
the single negative charge. These negative charges tend to blow the double
helix structure apart, which is perhaps why I haven't included them in the
forces that hold the helix together. Why is the helix stable?
Inside the cell, the monovalent salt concentration is about 0.15 M. The
major cation in the cell is K, while i... 阅读全帖
B******o
发帖数: 496
37
来自主题: Biology版 - SYBR real-time PCR误差很大?
Sunny, 你的说法不正确。Sybr和Taqman在sensitivity上是一样的, Sybr green与
double strand DNA结合,而taqman依靠PCR酶酶切活性来使原本quench的fluorescent
dye发光。 ABI常宣称说Taqman更specific,因为需要probe和target sequence结合才
会被PCR酶切到。实际上,这个hybridization的过程也有non-specific binding的,它
并没有提高PCR本身的sensitivity,而只是免去了看melting curve的必要. Sybr
Green的方法结合melting curve在sensitivity上和taqman一样。
我临时查了一下就发现了很多文章表达了同样的看法,http://mic.sgmjournals.org.proxy1.athensams.net/content/149/1/269.short
很多人的bias仅仅是公司特意宣传下被欺骗了而已。
前面ArtyArty说的很中肯,楼主连自己primer的线性扩增好不好都没测过,显然在很多... 阅读全帖
s******y
发帖数: 28562
38
来自主题: Biology版 - SYBR real-time PCR误差很大?
这个我不太同意。
首先,这两者的理论sensitivity的确差不多,而且在某种程度上来说,Sybr green因
为信号强度大,其实在检测上更容易被仪器看到。不过,你要是真的两者都做一次你就
知道了,对于含量特别低的样品,Sybr green的信号强度根本没有什么用,因为不同重
复孔跑出来的差别太大了(类似楼主看到的这个问题),导致那些跑出来的常常都是废
数据,所以真实的有效敏感度没有那么高。
另外,你可能对Taqman 的特异性的理解有些偏差。那个fluorescent probe, 要产生荧
光必须有两个条件:1,必须在template 上有PCR reaction 在进行; 2。probe 必须
能够结合在template 上面。必须两个条件同时满足,缺一不可,如果仅仅是probe非特
异结合是没有荧光的。
但是对于Sybr-green, 只要满足第一个条件就可以有信号了。
这样一来,Taqman qPCR 和Sybr-green 的特异性向比较就类似于Nested PCR 和普通
PCR的差别,前者在理论上肯定比后者更特异。
另外,Sybr-green 对于高Ct 的样品,光... 阅读全帖
m*********D
发帖数: 1727
39
我是snowyday说的老式酶切连接的老疙瘩了。你这个3KB应该是小菜一碟。上面有人提
到的Q5不错。那个GC-melt对付genomic DNA很好,唯一提醒的是primer(match的部分
)长一点,我一般25-28nt. 在加GC-melt的情况下,annealing的温度适当比用普通Taq
低点,3-5度。
genomc DNA可以用Qiagen的blood and tissue kit提取.一般12-well plate,一个well
就够用20-50次PCR了。
我一般把要PCR的fragment在网上扫描一下酶切位点,重点在don't cut和single cut
list。3kb应该很容易找到合适的位点。然后看自己的vector找合适的酶,加到pcr
primer的5’end。一般情况下,我会在酶点外再加4 nt,很多酶是不能切直接裸露在5’
外的位点的。所以,你的primer该是:4nt-酶点-25ntmatching seq. 3kb,很多情况下
可以用两个不同的酶来克隆。Sal I是老鼠和人genome里少见的酶位点,我几乎每次都
能用上。其他的看运气,识别8 ... 阅读全帖
x********e
发帖数: 35261
40
high resolution melting curve analysis?
把这段序列先p出来,然后比较ttaccaccactc与ttaccactc的melting curve区别。我没
做过,不知道需不需要特殊的仪器。
t******e
发帖数: 196
41
来自主题: Chemistry版 - 求文两篇
先谢谢了!
====================
【作者】Richard Owczarzy,* Yong You, Bernardo G. Moreira, Jeffrey A. Manthey
, Lingyan Huang, Mark A. Behlke, and Joseph A. Walder
【文题】Effects of Sodium Ions on DNA Duplex Oligomers: Improved
Predictions of Melting Temperatures
【期刊名,年份,卷(期),起止页码】Biochemistry, 2004, 43 (12), pp 3537–
3554
【全文链接】http://pubs.acs.org/doi/abs/10.1021/bi034621r
【求助者联系方式】h****[email protected]
=========================
【作者】Richard Owczarzy
【文题】Melting temperatures of nucleic acids: Discrepancies in an
j*********5
发帖数: 6221
42
来自主题: Chemistry版 - 问个DSC的问题
做一个小分子DSC,发现在melting和crystalizing两个比较大,sharp的峰,但是melt
之前有个小bump,结晶峰后也有个类似小bump,砖家们谁知道这是什么transition?
w********h
发帖数: 12367
43
来自主题: Chemistry版 - 问个DSC的问题
你这两个bump是往哪个方向bump的?是否和主峰相反?
相反的话,melt之前的结晶峰叫cold crystallization peak,
就是说,glass transition之后链活动能力增强,
导致部分链段重组形成有序的结晶结构,
然后在更高温度下熔化。
如果同一方向,是因为有杂质,或者结晶有多重结晶结构,
在高分子里比较常见的就是有不同链段长的同一类分子。
题外话:
在国内遇见两次询问“为什么你的XXX有俩峰而我的只有一个”这种问题,
问得态度也不友好。。。我只能心里觉得这学生的素质是不行啊。。。
还有人都来美国了,都不会问问题,
什么you JUST this, you JUST that...,听起来很牛叉的样子。

melt
b*****d
发帖数: 591
44
【 以下文字转载自 Macromolecules 讨论区 】
发信人: bigwood (kkk), 信区: Macromolecules
标 题: Job Position - Extrusion Process & Product Development Engineer
发信站: BBS 未名空间站 (Thu Jul 21 13:17:28 2011, 美东)
Company: Tepha, Inc. (www.tepha.com)
Location: Lexington, MA
Title: Extrusion Process & Product Development Engineer
Summary:
This position requires hands-on individual with fiber extrusion and
processing experience within the medical device industry. The individual
must be a self starter, able to work wi... 阅读全帖
f*****0
发帖数: 489
45
1206 or 0805 parts are fairly easy to solder: you just tin the pads a little
bit, use a tweezer to push the part onto the pads and use a fine tip to
melt the tin on the pads.
sot23 parts can be done this way too.
for multi-pin chips, I coat the pcb with solder paste (mostly solder but
also some flux), put on the part, and use a hotair gun to melt the paste.
the trick is to use the right amount of paste: if you used too much, you can
always wipe them off with a fine multi-strand copper wire. it d
p**h
发帖数: 18
46
来自主题: Law版 - which answer is correct?
B is right, saying it is not continuous covered by ice, which imply the ice
once melted 3 m years ago.
A is not good, only melted for a short period is consistent with "generally"
thought to have been covered by ice.

.
for
b**s
发帖数: 589
47
Finally, certain rheological measurements can often reveal the presence or
absence of block copolymer structures. For example, the melt viscosity of
block copolymers is usually considerably higher than that of a blend of
homopolymers of similar molecular weight. This is especially true for A-B-A
and -(A-B)n- structures due to the persistence of some degree of physical
network structure even in the melt. This effect is magnified in block
copolymers consisting of segments that are long and tha
r****r
发帖数: 14
48
Where is the endotherm? The equilibrium melting temperature for cis-1,4-PI is
around 35C. The real Tm will be 20C sth. If you store your sample at room
temperature, I don't think you can find a melting peak. Have you ever quenched
it to very low temperature like below 0C? Check the paper by PJ Phillips:
Macromolecules 1987, 20, 2138.
c*****e
发帖数: 238
49
来自主题: Macromolecules版 - 一个高分子科学的基本问题
Flory's prediction is only that the size of a polymer is the same, or
has the same scaling in theta-solvent as in melts.
And he did not say the same thing about other quantities,
like the scattering functions or correlation functions.
Thus the configuration of the polymer chain in the melt is not necessarily
the same as a simple random walk, there might be certain corrections to the
leading scaling, which are different from the solution media.
Personally I buy this, I think it is quite natural t
b*o
发帖数: 47
50
来自主题: Macromolecules版 - 一个高分子科学的基本问题
I am very interested in this and looking forward to your publication. In
Edwards' derivation, I think the attraction part has the physical origin of
the monomer space packing entropy de Gennes mentioned. The polymer chain in
melt (supposedly polymer good solvent, i.e., kai=0) and in theta solvent both
shrink from that in good monomer solvent, but with different physical driving
force: entropy in the melt case while enthalpy in the theta solvent case, I
think.

off.
=
correlation
of
interaction
i
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