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全部话题 - 话题: probing
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k***g
发帖数: 4904
1
Just copied a few of the many emails I got. This happend all the time and it
's unbelievable that there was a guy who did not even know it.
----------------------------------------------------------------------------
----
Receive $100 in Starbucks® gift cards,* or
get up to 50% off**
Save now
Place a Gibco® product order of $1,500 or more to
receive $100 in Starbucks® gift cards or get up to 50% off.
Select from over 1,000 qualifying products.
© 2014 Life Technologies Corpora... 阅读全帖
e**5
发帖数: 645
2
来自主题: LeisureTime版 - 牛顿的水桶1687-2011 zt
·方励之·
绝对的转动
经典物理的开山之作“自然哲学之数学原理”发表于1687年。书中,牛顿第一个讲到的
物理实验是水桶实验。
牛顿说,用一根长的软吊绳提一桶水,把吊绳拧成麻花状。如果你握住吊绳,不让麻花
状的绳子松开,桶及桶中的水是相对是静止的,水面是平的。突然放开手,麻花开始放
松,吊绳旋转,水桶也随着吊绳旋动。最初,桶中的水并不转动,只有桶在旋转,桶和
桶中的水有相对转动。慢慢地,水被桶带动,也开始转动。最后,水和桶一样转动。这
时,水和桶之间又是相对静止的,不转动的。但水面却呈凹状,中心低,桶边高。牛顿
爵士特别说“ I have experienced”。他亲自做过这实验。
这个实验很容易,任何有水桶和软绳的人都可以试试。我也多次做过这个实验。 1957
冬 - 1958年春, 我在河北省赞皇县南邢郭村下放劳动。天天要用软吊绳的桶从约十米
深的井中打水。水桶的姿态只能用软吊绳控制。没有十天半个月的练习,是学不会水桶
姿态控制的。结果是,任凭你让吊桶十五次七上八下,每次提上来的水,大多不过是半
桶水,而且在旋转。常被同吃同住同劳动的老农(其实不老,同我年龄相仿,但农活经
验老道... 阅读全帖
B****m
发帖数: 63
3
用过一次affymetrix的芯片,很不喜欢。确实很多probe set没有明确标注。对于这些
probe set,你可以到affymetrix的官方网站上搜索信息(需要注册)。在他们的官网
上,每个probe set都会有个链接,连到gemome browser上。我的经验是,如果官网上
实在没有这个probe set的信息,但是在genmome browser上 这个probe set 覆盖了基
因A的一部分,那我就手动把这个probe set的对应基因标注为基因A。 做这个手动工作
很烦人,所以手动查询一下变化倍数大的为止probe set 就行了,变化小的不管。
将probe set的变化转化成基因后,要用gene ontology对这些基因进行功能分类,进而
信号通路分析。
C********g
发帖数: 9656
4
【 以下文字转载自 History 讨论区 】
发信人: Communipig (共产猪), 信区: History
标 题: 方励之:牛顿的水桶1687-2011
发信站: BBS 未名空间站 (Fri Sep 23 15:54:24 2011, 美东)
http://my.cnd.org/modules/wfsection/article.php?articleid=30039
绝对的转动
经典物理的开山之作“自然哲学之数学原理”发表于1687年。书中,牛顿第一个讲到的
物理实验是水桶实验。
牛顿说,用一根长的软吊绳提一桶水,把吊绳拧成麻花状。如果你握住吊绳,不让麻花
状的绳子松开,桶及桶中的水是相对是静止的,水面是平的。突然放开手,麻花开始放
松,吊绳旋转,水桶也随着吊绳旋动。最初,桶中的水并不转动,只有桶在旋转,桶和
桶中的水有相对转动。慢慢地,水被桶带动,也开始转动。最后,水和桶一样转动。这
时,水和桶之间又是相对静止的,不转动的。但水面却呈凹状,中心低,桶边高。牛顿
爵士特别说“ I have experienced”。他亲自做过这实验。
这个实验很容易,任何有水桶和软绳... 阅读全帖
m****m
发帖数: 2211
5
来自主题: Science版 - 方励之:牛顿的水桶1687-2011
【 以下文字转载自 History 讨论区 】
发信人: Communipig (共产猪), 信区: History
标 题: 方励之:牛顿的水桶1687-2011
发信站: BBS 未名空间站 (Fri Sep 23 15:54:24 2011, 美东)
http://my.cnd.org/modules/wfsection/article.php?articleid=30039
绝对的转动
经典物理的开山之作“自然哲学之数学原理”发表于1687年。书中,牛顿第一个讲到的
物理实验是水桶实验。
牛顿说,用一根长的软吊绳提一桶水,把吊绳拧成麻花状。如果你握住吊绳,不让麻花
状的绳子松开,桶及桶中的水是相对是静止的,水面是平的。突然放开手,麻花开始放
松,吊绳旋转,水桶也随着吊绳旋动。最初,桶中的水并不转动,只有桶在旋转,桶和
桶中的水有相对转动。慢慢地,水被桶带动,也开始转动。最后,水和桶一样转动。这
时,水和桶之间又是相对静止的,不转动的。但水面却呈凹状,中心低,桶边高。牛顿
爵士特别说“ I have experienced”。他亲自做过这实验。
这个实验很容易,任何有水桶和软绳... 阅读全帖
f**d
发帖数: 768
6
来自主题: Neuroscience版 - eBook: From computer to brain
这是一本计算神经科学的优秀著作,全文拷贝这里(图和公式缺),有兴趣的同学可以
阅读
如需要,我可以分享PDF文件(--仅供个人学习,无商业用途)
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖
g********n
发帖数: 2314
7
来自主题: _GoldenrainClub版 - 方励之:牛顿的水桶1687-2011 (转载)
【 以下文字转载自 History 讨论区 】
发信人: Communipig (共产猪), 信区: History
标 题: 方励之:牛顿的水桶1687-2011
发信站: BBS 未名空间站 (Fri Sep 23 15:54:24 2011, 美东)
http://my.cnd.org/modules/wfsection/article.php?articleid=30039
绝对的转动
经典物理的开山之作“自然哲学之数学原理”发表于1687年。书中,牛顿第一个讲到的
物理实验是水桶实验。
牛顿说,用一根长的软吊绳提一桶水,把吊绳拧成麻花状。如果你握住吊绳,不让麻花
状的绳子松开,桶及桶中的水是相对是静止的,水面是平的。突然放开手,麻花开始放
松,吊绳旋转,水桶也随着吊绳旋动。最初,桶中的水并不转动,只有桶在旋转,桶和
桶中的水有相对转动。慢慢地,水被桶带动,也开始转动。最后,水和桶一样转动。这
时,水和桶之间又是相对静止的,不转动的。但水面却呈凹状,中心低,桶边高。牛顿
爵士特别说“ I have experienced”。他亲自做过这实验。
这个实验很容易,任何有水桶和软绳... 阅读全帖
h***y
发帖数: 4936
8
这才是俄罗斯新闻社26号的新闻,里面提到的原因是“the formation of plasma on
the probe whilst inside the Earth's magnetosphere may have caused the device
to fail. ”
明报难道在香港注册了一个野鸡机构也叫“俄罗斯新闻社”?
----------------------------------------------------------
http://rt.com/news/prime-time/plasma-phobos-probe-793/
Space oddity: Plasma cloud responsible for Phobos mission failure?
Published: 26 January, 2012, 21:35
The answer to the mysterious failure of the Phobos mission may now be close
at hand. An inter-departmental commissio... 阅读全帖
H*******g
发帖数: 321
9
继续剧透...
话说进了基地后发现空无一人,机器人也不见了。在一个实验室发现了屋子正中间有一
个圆球,角落有一个小方盒子。后来破解了基地的日志,知道这群机器人一直在等天
朝的指示,后来还自己出发去搞探索,发现了“宝藏”。回来之后一阵发明创造,然后
又出去了,结果就再也没回来。那个小盒子就是他们研制的制造重力的装置。于是他们
觉得把这个东西带回地球去应该能救大家,而且还发现其实这个星球也不稳定,十年内
就会被黑洞吞噬。于是大家决定回家。
出了基地,结果碰到了留守的天朝机器人。这几个机器人为了不让他们把有天朝知识产
权的重力盒子带回去,跟他们打斗起来。我方机器人挺身而出,让其他人得以脱身爬出
去找到飞船。结果飞船已经被另外一个天朝机器人破坏了...把这个坏机器人摆平后,
一个男配提出飞船貌似还能飞,就是需要先升到空中。于是他提出自己坐天朝留下的一
个单人飞船飞到空中,开启重地装置,把飞船吸起来,然后就可以飞走了。同时他们可
以把盒子的程序弄出来存在probe里面,可以带回地球。于是又回到基地往probe里面存
东西。等弄好之后要上飞船了,之前留下来勇斗天朝机器人的我方机器人浑身是伤的胜
利... 阅读全帖
h******y
发帖数: 351
10
我的建议是,在和老板争论是否有deletion之前,利用其他的办法证实你的PCR所发现
的结果。PCR是很容易出错的,如果你能利用Southern证实homozygous的deletion确实
存在,相信你的老板是会接受你的结论的。
Constance L. Cepko实验室最近retract了G&D上的一篇文章,很好的说明了PCR的易错
性。有兴趣的可以看看。
http://genesdev.cshlp.org.mutex.gmu.edu/content/25/12/1344.long
“We are writing to clarify the interpretation of the results from our above
-mentioned paper. In this study, we used two methods to examine the
structure of RNA from the mouse Math5 (Atoh7) locus. Our initial
characterization was an analysis of the Math... 阅读全帖
g**a
发帖数: 2129
11
1.what is the platform for your expression data: RNA-seq or expression assay
. For RNA-seq, it is count data. So what ever you guys talked about are
meaningless. Let's assume it is expression assay.
2.Based on assumption in 1, the expression value you were talking about, are
they value/probe or value/gene? If it is value/gene, how did you combine
the reading from different probes within one gene? It is not that easy to
combine the expression from multiple probes in one gene, just like combine
di... 阅读全帖
s******r
发帖数: 5309
12
来自主题: Military版 - 疮破新代号“Individual 1”
1号在竞选期间具体指挥了和普京高层的接触。
1号在2016年亲自参与勾结俄国情报部门偷窃民主党电邮,干涉美国大选。
死定了。
In two major developments this week, President Trump has been labeled in the
parlance of criminal investigations as a major subject of interest,
complete with an opaque legal code name: “Individual 1.”
New evidence from two separate fronts of special counsel Robert S. Mueller
III’s investigation casts fresh doubts on Trump’s version of key events
involving Russia, signaling potential political and legal peril for the
president. Inves... 阅读全帖

发帖数: 1
13
【 以下文字转载自 Military 讨论区 】
发信人: sgrastar (人马座A星), 信区: Military
标 题: 疮破新代号“Individual 1”
发信站: BBS 未名空间站 (Thu Nov 29 21:28:47 2018, 美东)
1号在竞选期间具体指挥了和普京高层的接触。
1号在2016年亲自参与勾结俄国情报部门偷窃民主党电邮,干涉美国大选。
死定了。
In two major developments this week, President Trump has been labeled in the
parlance of criminal investigations as a major subject of interest,
complete with an opaque legal code name: “Individual 1.”
New evidence from two separate fronts of special counsel Robert S. Mueller
III’s investigation casts f... 阅读全帖
k****w
发帖数: 3753
14
来自主题: E-Sports版 - 泪奔,我找到打z的宝典了
原来要大前期micro压制
From Inflowmini, now #2 in 北美服
原贴:http://us.battle.net/sc2/en/forum/topic/1009691265
这哥们儿很喜欢速双开。
原文:
By "my" I mean ones I do, I am not claiming I came up with them.
Cleaned this thread a bit, added some more recent replays, and took off some
builds that are unstable, and added some more openings I do.
HD quality stream : http://www.teamliquid.net/video/userstream.php?user=Minigun
Am also coaching for 15$/h email @ M********[email protected]
I am currently trying to get my stream feature... 阅读全帖
t*****o
发帖数: 4919
15
玩万用表被电死了, 9V电池
so you get the answer now.
(1999) A US Navy safety publication describes injuries incurred while doing
don't's. One page described the fate of a sailor playing with a multimeter
in an unauthorized manner. He was curious about the resistance level of the
human body. He had a Simpson 260 multimeter, a small unit powered by a 9-
volt battery. That may not seem powerful enough to be dangerous… but it can
be deadly in the wrong hands.
The sailor took a probe in each hand to measure his... 阅读全帖
b********E
发帖数: 121
16
这两个都不行,因为至少wifi的载波都可以是5G多了,你这些设备根本测不到wifi的辐
射。 要想测家里的大多数设备的辐射,你需要谱分析仪+Magnetic Field Probe+
Electric Field Probe
比较便宜的方案:
TSA5G35 Mini Spectrum Analyzer 能测1Mhz to 5.35GHz http://www.triarchytech.com
和一套啊Near Field Probe(Magnetic Field Probe+Electric Field Probe):
Beehive EMC Probes: http://www.tequipment.net/Beehive101A.html
a***e
发帖数: 1010
17
(1)try p32 probe, that's better than dig-probe. try use StarFire kit to
increase the sensitivity of your probe (~10x).
(2)40-50 nt probe is more than enough. People can usually blot small RNAs
with 20-30 nt probes (after StarFire labeling)
(3) you can try Tagman-qPCR or splinted-ligation assay, or LNA probe to do
northern blot
(4) deep sequencing w/ or w/o cloning
h******y
发帖数: 351
18
来自主题: Biology版 - RNA原位杂交问题请教
RNA-RNA has a higher Tm than RNA-DNA, so you can use more stringent washing
in your in situ and get better background. In a nutshell, RNA probe is
better than DNA probe. Of course, your RNA probes should be free of any
RNase contamination.
Current commercially available DIG labeling kits is as sensitive as
radioactive labeling kits, although the advantage of using radioactive-
labeled probes is that you can do quantification analysis by densitometry.
The disadvantage of radioactive-labeled pro... 阅读全帖
Z**R
发帖数: 1233
19
来自主题: Military版 - 【转】Akatsuki金星入轨失败
刹车失灵?
http://www.bbc.co.uk/news/world-asia-pacific-11945244
Japan's Akatsuki probe fails to enter Venus orbit
Japan's first space probe bound for Venus has failed to enter the planet's
orbit, the country's space agency says.
The space craft, Akatsuki, is believed to have passed Venus after it failed
to slow down sufficiently.
Akatsuki, launched about 200 days ago, fired its main engine just before
0000 GMT on Monday to allow the planet's gravity to capture the probe.
A previous interplanetary sp... 阅读全帖
w*******e
发帖数: 15912
20
文章提交者:忙碌一天 加贴在 陆军论坛 铁血论坛 http://bbs.tiexue.net/bbs32-0-1.html
印度抢先中国一步,成功向火星发射探测器,随后北京呼吁“共同努力”以确保外太空
和平。11月5日下午,火箭搭载着“曼加里安”号飞行器升空,使得印度成为排在美国
、俄罗斯和欧洲之后第四个向火星发射探测器的国家。预计明年9月进入红色星球的轨
道。“外太空是全人类的共同财富,各国拥有和平探索和利用外太空的权利。”中国外
交部发言人洪磊在新闻发布会上被问及如何看待印度探测火星时说。同时,他表示国际
社会应共同努力,维护外太空的永久和平与可持续发展。
外文标题:China calls for 'joint efforts' after India's Mars mission
[ 转自铁血社区 http://bbs.tiexue.net/ ]
外文地址:http://timesofindia.indiatimes.com/world/china/China-calls-for-joint-efforts-after-Indias-Mars-mission/articlesho... 阅读全帖
l****z
发帖数: 29846
21
来自主题: USANews版 - Cuomo童鞋有麻烦啦
Manhattan US Attorney Preet Bharara has expanded his anti-corruption crusade
to the far western edge of the state — investigating Gov. Andrew Cuomo’s
controversial Buffalo Billion revitalization project, The Post has learned.
The probe of Cuomo’s pet project is the first to touch his office beyond
Bharara’s investigation of the governor’s shutdown of the Moreland
Commission to Investigate Public Corruption last year.
The new investigation is focused on the multimillion-dollar contracts
awarded t... 阅读全帖
S*******i
发帖数: 2018
22
来自主题: USANews版 - Democrats, Russians and the FBI
It turns out that Russia has sown distrust in the U.S. political system—
aided and abetted by the Democratic Party, and perhaps the FBI. This is an
about-face from the dominant media narrative of the last year, and it
requires a full investigation.
The Washington Post revealed Tuesday that the Hillary Clinton campaign and
Democratic National Committee jointly paid for that infamous “dossier”
full of Russian disinformation against Donald Trump. They filtered the
payments through a U.S. law firm (... 阅读全帖
i***e
发帖数: 9429
23
来自主题: Living版 - 住家冷气机自行保养(2014)
再次提醒大家需要定期换FURNACE/Fan Coil Filter。
比起去年的冷气机保养贴,今年做了小小改动,将会用到高效率的清洗工具,这个清洗
工具虽然有点小贵,我觉得是蛮值得投资的。
需要用到的一个工具是:
下面清洗工具A和B 选一个即可。
A:)SpeedClean Condenser Needle Condenser Coil Cleaner(140刀左右)
http://www.speedclean.com/products/condenserneedle.aspx
还需要一个yellow 15-degree spray nozzle
B:)Nu-Calgon Coil Gun with Coil Gun Probe(Coil Gun + Gun Probe, 140刀左右)
http://www.nucalgon.com/products/coil-cleaners/sprayers/coil-gu
C:)DIY Condenser Needle/ Coil Gun Probe (需要有点动手能力)
我已找到了合适的材料,在试制中,DIY 成本在35刀左右,汗... 阅读全帖
g**********2
发帖数: 2408
24
来自主题: Stock版 - BIDU,SINA,EDU要delist了
这个分析很有意思。呵呵
我昨天就分析了,这九家公司不可能包括新东方
Web Stocks Slip on Delisting Concerns: China Overnight
By Belinda Cao & Victoria Stilwell - Dec 4, 2012 5:11 PM GMT-0500
Facebook Share
LinkedIn
Google +1
1 Comment
Print
QUEUE
Q
Most Chinese stocks fell in New York, led by Internet companies, after U.S.
regulators accused units of the Big Four auditors of refusing to cooperate
on a fraud probe of China-based companies.
The Bloomberg China-US Equity Index of the most-traded Chinese shares in the
U.S. dipped 0.1 ... 阅读全帖
w***g
发帖数: 5958
25
正好我也是LSH专家。这个论文的方法我觉得:
1. 没法在GPU上实现,只能在CPU上实现。如果在GPU上做,他用LSH加速的
那部分计算iteration速度至少下降两倍。如果要evaluate出来他这个95%,
只能是算浮点数乘法的计算次数,但是随之来的是非乘法计算的增加,
cache hit rate降低,单次乘法速度变慢。这个牛吹得太大了。
2. 目前这个规模的网络用LSH是很难加速的,并且对准确度损失会很大。
并且我在2011年左右就已经实现了用社交网络算法吊打LSH并且对k-NN搜索
社区完成了拨乱反正。Benchmark在此:
https://github.com/erikbern/ann-benchmarks
kgraph是我的项目。最高那条线是个毛子,走的是我的路线。但我确实干不过他。
这paper很可能就是用了我implement的multi-probe LSH。
发明multi-probe的是我师姐, 但她的代码没公开。所以现在能找到的最
标准的multi-probe实现是我的版本。multi-probe针对的是内存不足的情况。
绝大部分引用multi-probe... 阅读全帖
G******t
发帖数: 90
26
【 以下文字转载自 Military 讨论区 】
发信人: wsnonline (卫所南次郎-子曰:“再得瑟我削你啊!”), 信区: Military
标 题: 中国批印度火星计划,三哥网民激动回复
发信站: BBS 未名空间站 (Fri Nov 8 22:27:15 2013, 美东)
文章提交者:忙碌一天 加贴在 陆军论坛 铁血论坛 http://bbs.tiexue.net/bbs32-0-1.html
印度抢先中国一步,成功向火星发射探测器,随后北京呼吁“共同努力”以确保外太空
和平。11月5日下午,火箭搭载着“曼加里安”号飞行器升空,使得印度成为排在美国
、俄罗斯和欧洲之后第四个向火星发射探测器的国家。预计明年9月进入红色星球的轨
道。“外太空是全人类的共同财富,各国拥有和平探索和利用外太空的权利。”中国外
交部发言人洪磊在新闻发布会上被问及如何看待印度探测火星时说。同时,他表示国际
社会应共同努力,维护外太空的永久和平与可持续发展。
外文标题:China calls for 'joint efforts' after India's Mars mission
[ 转自铁... 阅读全帖
H*******g
发帖数: 321
27
看trailor跟这个泄漏的剧本很像啊
地球环境恶化,国家不复存在(貌似中美打了一仗?),人口锐减,剩下的人聚集在农
庄以种玉米为生。因为别的作物都长不了了,而且貌似玉米也开始出现问题了。
男主是个不得志的电子机械工程师,带着两个儿子和他岳父过活。有个儿子和他老爸志
向很投合,对学校教的那套自欺欺人的东西不感冒。男主被人叫去修东西顺便发现了个
天上掉下来的probe,后来被probe带到一个小岛发现一个秘密基地,遇到女主和她爸。
这群人躲在这里秘密研发,打算去一个几十年前发现的虫洞搞穿越,因为他们觉得这个
虫洞这么突然出现肯定是有外星人或造物主在暗示帮助他们逃离地球。带回来的那个
probe实际上是美国几十年前放出去的,返回的信息告诉他们有一个冰冻的星球在虫洞
那头,可能有水和氧气,适合人类生存。
一番挣扎后,男主决定去冒险拯救自己的家庭。临走答应儿子一定会回来。于是男主女
主另外两个科学家还有两个机器人就出发啦。顺利达到一个直径只有4米的虫洞...把飞
船塞进虫洞后,发生了一种神奇的现象,然后就出来了。计划的路线是沿着一条很危险
的路线从两个黑洞中间穿过到达冰冻星球,结果有个科学家同... 阅读全帖
w*******e
发帖数: 15912
28
【 以下文字转载自 Military 讨论区 】
发信人: wsnonline (卫所南次郎-子曰:“再得瑟我削你啊!”), 信区: Military
标 题: 中国批印度火星计划,三哥网民激动回复
发信站: BBS 未名空间站 (Fri Nov 8 22:27:15 2013, 美东)
文章提交者:忙碌一天 加贴在 陆军论坛 铁血论坛 http://bbs.tiexue.net/bbs32-0-1.html
印度抢先中国一步,成功向火星发射探测器,随后北京呼吁“共同努力”以确保外太空
和平。11月5日下午,火箭搭载着“曼加里安”号飞行器升空,使得印度成为排在美国
、俄罗斯和欧洲之后第四个向火星发射探测器的国家。预计明年9月进入红色星球的轨
道。“外太空是全人类的共同财富,各国拥有和平探索和利用外太空的权利。”中国外
交部发言人洪磊在新闻发布会上被问及如何看待印度探测火星时说。同时,他表示国际
社会应共同努力,维护外太空的永久和平与可持续发展。
外文标题:China calls for 'joint efforts' after India's Mars mission
[ 转自铁... 阅读全帖
c*******n
发帖数: 671
29
谢谢,以下是我的X11/xorg.0.log, 我看不出来什么问题?是的我不应该删lightdm,
不是那个的问题。startx会出现我上面帖子写的Loading extension GLX, fatal
server error: no screen found. 请问我该怎么修复xwindow? 谢谢!
[ 4.113]
X.Org X Server 1.13.0
Release Date: 2012-09-05
[ 4.113] X Protocol Version 11, Revision 0
[ 4.113] Build Operating System: Linux 3.2.0-37-generic x86_64 Ubuntu
[ 4.113] Current Operating System: Linux x-ThinkPad-X201 3.5.0-49-generic
#74-Ubuntu SMP Fri May 2 23:28:58 UTC 2014 x86_64
[ 4.113] Kernel command line: BOOT_IM... 阅读全帖

发帖数: 1
30
来自主题: Hardware版 - 海康威视的摄像头太烂了
谁叫自己上了嵌入式的贼船,工资低呢。。。C看多了,也不能刷题转码搞互联网
为什么摄像头领域不能有像开源openwrt一样的项目呢?
把海康的dmesg贴出来,大家可以参考,他们用安霸S3L,https://www.ambarella.com/
products/security-ip-cameras/security-ip-camera-products#S3L
[ 0.000000] Booting Linux on physical CPU 0x0
[ 0.000000] Linux version 3.10.73+ ([email protected]) (gcc version 5
.2.1 20151005 (Linaro GCC 5.2-2015.11-2) ) #1 PREEMPT Mon Jan 23 10:38:22
CST 2017
[ 0.000000] CPU: ARMv7 Processor [414fc091] revision 1 (ARMv7), cr=
10c53c7d
[ 0.000000] CPU: PIPT / ... 阅读全帖
n***h
发帖数: 11
31
来自主题: Biology版 - Re: [Question 1] About microarray
No. It's like the other way around...
Affymetrix chips use synthesized 25-base long oligonucleotides as probes,
including perfect match and mis-match probes. A set (16-20 ) of probes are
designated to detect one transcript. So all the probes on the chip stand
for known genes, which is nice for reproducibility and further analysis of
your data, while could be a limitation too: you can only detect the expression
level of the probes they provide.
I haven't worked with the so-called "arrays", but as
z**m
发帖数: 50
32
本人生物菜鸟,请教版上牛人,万分感谢!!!
想检测老鼠体外培养细胞中病毒感染前期,病毒RNA是否和目标蛋白结合成RNP complex。
目标蛋白IP抗体available,想检测的viral RNA 为40-50 nt的leader sequence (
LeRNA)。
做了些homework,准备用目标蛋白pulldown,PK处理,纯化RNA组分,northern检测。
问题:
1)由于个人原因,不想用同位素标记探真做northern,不知可否用dig-probe做
northern,主要担心RNA量太少,不知道dig的灵敏度是否足够。
2)由于目标RNA只有40-50 nt,请问northern 的DNA或RNA probe是否只能是这40-
50nt的序列?如果probe只有40-50 nt,是否太短?尤其是用dig-UTP标记?可否适当
延长probe至200 nt-300nt(后续sequence是病毒的另外一个高表达基因,如果该基因
mRNA也和目标蛋白结合最好,如果不和目标蛋白结合,是否延长的probe检测不到40-50nt的leRNA?)
3)除了northern,
s******s
发帖数: 13035
33
来自主题: Biology版 - 求分离x chromosome的idea
打算分离x chromosom,研究上面的protein components。以前没做过这样的活, 求点
idea.
有什么specific的办法分离么?还是必须用beads连到DNA probes上,然后用DNA probe
拉?
一个probe拉的下来么? 还是必须粉碎了以后用N个不同区域的probe?
probe杂交的时候变性会不会把上面的protein都搞掉了?
多谢了
s****9
发帖数: 932
34
I am also doing in situ with DIG probe. None of the probes from my designs
show any signal while other probes from other lab works very well as
positive control. Do you have any suggestion for probe design?
The mRNA is 1.5kb. I desgined three probes, full length, C-terminal half (
700bp), and N terminal half (700bp).
s*********8
发帖数: 901
35
NEW YORK – An insider trading case last year that federal authorities said
was the biggest ever is providing a recipe for another case that may be even
bigger.
The current case is largely an extension of work that led to the arrest of
Galleon Group founder Raj Rajaratnam in October 2009. The Galleon
investigation marked the first time that federal authorities used wiretaps
in an insider trading probe.
Similarly, wiretaps led to the first arrest in the latest case. Don Ching
Trang Chu, a consulti... 阅读全帖
b***y
发帖数: 14281
36
来自主题: Military版 - 方励之的学术成就全记录
既然有人争论方的学术水平,就上网搜了一下。应该说这个水平对于一个junior
faculty来说可以算是很牛B了,但是对于老方这种senior的已经搞了一辈子的人来说,
就只能算so so。不要说是院士,离aps fellow的级别也还差了不少。引用率最高的一
篇文章还是95年到了arizona之后跟老外合作的,被引118次,这也是方唯一一篇被引用
上百次的文章。可见方励之89之前确实没有做出过什么重要的工作,他的名声和地位绝
对是靠政治得来的,当然其中部分的也是80年在中国搞科普所得,必须承认科普工作也是
有重要的社会意义的。
(BTW,有人说你这个搜索未必完整。不错,确实不能保证100%毫无遗漏。但是我
用的search engine is THE search engine everybody in this community uses today.
So, if any article is not found by this search engine, sadly, it simply doesn't matter,
because no one would ever n... 阅读全帖
C*I
发帖数: 4736
37
多行不义必自毙。 看好了,都要进去了。
http://www.scmp.com/sites/default/files/styles/486x302/public/2013/08/30/bo_zhou.jpg?itok=H55U7hfM
Former China Politburo member faces corruption probe: report
Reuters) - China's senior leadership has agreed to open a corruption
investigation into Zhou Yongkang, one of China's most powerful politicians
in the past decade, stepping up its anti-graft campaign, the South China
Morning Post reported on Friday.
The move against Zhou - a retired member of the Politburo's all-powerf... 阅读全帖
G*******n
发帖数: 6889
38
http://news.xinhuanet.com/english/2016-01/15/c_135010577.htm
Source: Xinhua 2016-01-15 01:00:46 [More]
BEIJING, Jan. 14 (Xinhua) -- China has officially begun a new round of lunar
exploration and will send the Chang'e-4 probe to the far side of the moon
in 2018, China's State Administration of Science, Technology and Industry
for National Defense (SASTIND) announced Thursday.
The far side of the moon is never visible to Earth because of gravitational
forces and has never been explored by h... 阅读全帖
G*******n
发帖数: 6889
39
来自主题: Military版 - 中国2020年登陆火星
http://gbtimes.com/china/china-racing-make-2020-launch-window-m
Andrew Jones
2016/02/22
Mars Exploration Rover Spirit captures a stunning view as the sun sank below
the rim of the Gusev crater on Mars on May 19, 2005.Mars Exploration Rover
Spirit captures a stunning view as the sun sank below the rim of the Gusev
crater on Mars on May 19, 2005. (Photo: NASA/JPL/Texas A&M/Cornell)
Tags:
China's space programMarsNational Space Science CenterChina Aerospace
Science and Technology CorporationEur... 阅读全帖
s******r
发帖数: 5309
40
GOP fundraiser Broidy under investigation for alleged effort to sell
government influence, people familiar with probe say
By Carol D. Leonnig
, Reporter
Devlin Barrett
, Reporter
Ellen Nakashima
, Reporter
Josh Dawsey
, White House reporter
August 17 at 8:43 PM
The Justice Department is investigating whether longtime Republican
fundraiser Elliott Broidy sought to sell his influence with the Trump
administration by offering to deliver U.S. government actions for foreign
officials in exchange for ... 阅读全帖
s******r
发帖数: 5309
41
疮破从1980年开始通过卖房帮俄国黑手党洗钱,因为疮破公司内部保守机密所
以FBI调查一无所获。这次疮破集团的大管家和白手套叛变,这长达几十年的犯罪事实
再也包不住了。
疮破唯一可能不蹲监狱的办法就剩出逃俄罗斯了。LOL
President Trump should be more worried about prosecutors in New York than
about the ongoing Russia probe led by special counsel Robert S. Mueller III,
retired Harvard Law School professor Alan Dershowitz said Sunday.
Dershowitz, an informal Trump adviser, said in an appearance on ABC News’s
“This Week” that the expanding probe by prosecutors in the Southern
District of New York could sp... 阅读全帖
l****z
发帖数: 29846
42
Chu takes responsibility for a loan deal that put more taxpayer money at
risk in Solyndra
By Carol D. Leonnig and and Joe Stephens, Published: September 29
Energy Secretary Steven Chu acknowledged Thursday making the final decision
to allow a struggling solar company to continue receiving taxpayer money
after it had technically defaulted on a $535 million federal loan guaranteed
by his agency.
Chu spokesman Damien La­Vera said in a statement that the secretary
approved the restructuring agr... 阅读全帖
l****z
发帖数: 29846
43
by Jammie
His father reportedly has asked for a reduced sentence but nobody could
understand what he said.
Sneed has learned a plea deal is now on the table between former Rep.
Jesse Jackson Jr. and federal authorities probing allegations of campaign
fund misuse.
Sneed is told the plea deal includes Jackson serving time in federal
prison.
“Significant jail time is now definitely a part of the deal,” said a
top Sneed source close to the probe.
“But I think [Jackson’s wife] Sandi, ... 阅读全帖
k****k
发帖数: 3322
44
资本主义与官僚共产主义的巧妙结合,多么的讽刺。
现在的中国,说白了就是那党中央元老们几百家控制的中国。在这儿生活,是帮资本家
打工,做得好当个小资本家;回国发展,那是帮二代们打工,有什么意义?
HONG KONG (Reuters) - A U.S. banking regulator's probe into JPMorgan's
hiring practices in China will have rival banks scrambling to review their
own records, lawyers say, in a market where ties to political and business
leaders can be key to winning big deals.
Banks around the world commonly hire people with government connections, but
this is especially prevalent in China due to the role the ruling... 阅读全帖
T**********e
发帖数: 29576
45
连多年前俄国人买老头房子都查,
Mueller Expands Probe to Trump
Business Transactions
The U.S. special counsel investigating possible ties between the聽Donald
Trumpcampaign and Russia in last year's election is examining a broad range
of transactions involving Trump's businesses as well as those of his
associates, according to a person familiar with the probe.
The president told the New York Times on Wednesday that any digging into
matters beyond Russia would be out of bounds. Trump's businesses have
involved Rus... 阅读全帖
j*********r
发帖数: 24733
46
House Republicans launch new probes into Obama-era Uranium One deal, FBI
handling of Clinton case
House Republicans on Tuesday launched new probes into several Obama-era
controversies, covering both the Justice Department’s 2016 handling of the
Clinton email case and the administration’s 2010 approval for the sale of a
mining company that gave the Russians partial control over American uranium
reserves.
House Intelligence Committee Chairman Devin Nunes, R-Calif., announced his
committee and the ... 阅读全帖
A**l
发帖数: 2650
47
来自主题: Automobile版 - 南湾的autozone给免费查error code么?
我靠,随手上autozone搜了下 code reader,这个入门级和专业级别的差价也忒大了啊
Code Reader
Showing 1 - 10 of 50 ItemsNEXT Page of 5 | Show All
Sort by Price: High Low
Actron/OBD I code scanner
(0 reviews)
Part Number: CP9001
Category: Scanner
STORE PICK UP
AVAILABLE
SHIP TO HOME
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Price: $29.99
Innova/OBD II CarScan Code Reader for all 1996 and newer European, Asian,
domestic, 2010 and newer diesel and hybrid compliance
(0 reviews)
Part Number: INN30203
Category: Scanner
STORE PICK UP
SPEC... 阅读全帖

发帖数: 1
48
Job linking: https://www.ornl.gov/careers
Job Posting Title: Postdoctoral Research Associate in Synthesis and
Heterogeneity of 2D Materials / NB50648234
Posted Date 12/14/2017; End Posting Date 02/14/2018
Purpose
We are looking for a Postdoctoral Research Associate to focus on the
synthesis and heterogeneity of two-dimensional (2D) materials. As a postdoc,
you will support in-house science projects at the Center for Nanophase
Materials Science (CNMS) that focus on how heterogeneity in 2D materia... 阅读全帖
x*******6
发帖数: 994
49
来自主题: JobMarket版 - job opening: Optics Thin Film Coatings
请直接联系recruiter
Job Description
Job responsibilities include:
*** Deposition of thin film coatings using vacuum coating equipment
*** Processing (e.g. cutting, heating, labeling) of coated substrate
samples
*** Making measurements of various physical properties (e.g. spectral or
color properties, ellipsometric properties via variable angle spectroscopic
ellipsometer - VASE, electrical properties such as sheet resistance via four
-point resistance probe and carrier density and carrier mobility ... 阅读全帖

发帖数: 1
50
Job linking: https://www.ornl.gov/careers
Job Posting Title: Postdoctoral Research Associate in Synthesis and
Heterogeneity of 2D Materials / NB50648234
Posted Date 12/14/2017; End Posting Date 02/14/2018
Purpose
We are looking for a Postdoctoral Research Associate to focus on the
synthesis and heterogeneity of two-dimensional (2D) materials. As a postdoc,
you will support in-house science projects at the Center for Nanophase
Materials Science (CNMS) that focus on how heterogeneity in 2D materia... 阅读全帖
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