l**********1
发帖数: 5204 | 1 not only Biology but Chemistry:
please go to
//www.ncbi.nlm.nih.gov/pubmed/20929839
or
//www.ncbi.nlm.nih.gov/pubmed/21310963 |
|
|
l**********1
发帖数: 5204 | 3 让你老板买个半自动或全自动工作站做Y-2-H 不就得拉
或借用同校里的其它单元的那设备
没听说过欧美高通量Y-2-H 筛选用一双手做的
半自动工作站 96 well
please below link:
//www.tecan.com/platform/content/element/5135/Tecan_Journal_09_01.pdf
for one
France developed a semi- automated yeast two-hybrid system with the Te-
MOTM 96 Multichannel Pipetting Option from Tecan, halving the time taken to
obtain reproducible results from six to three days.
if try Y-1-H
全自动工作站 384 well
please refer last Oct. Nature Methods two papers:
//www.ncbi.nlm.nih.gov/pubmed/22037705
/... 阅读全帖 |
|
l**********1
发帖数: 5204 | 4 Please send pdf file to mail address:
web link:
//onlinelibrary.wiley.com/doi/10.1111/j.1365-2265.1992.tb01481.x/pdf
PubMed:
//www.ncbi.nlm.nih.gov/pubmed/1286520
Abstract link:
//onlinelibrary.wiley.com/doi/10.1111/j.1365-2265.1992.tb01481.x/abstract
Thanks so much in advance. |
|
|
l**********1
发帖数: 5204 | 6 Bingo
plus 病毒proteins ICP0 to remodel viral chromatin Circadian CLOCK
PNAS 2010
web link:
//www.ncbi.nlm.nih.gov/pubmed/20876123
and J Virol. (2011) web link:
//www.ncbi.nlm.nih.gov/pubmed/21734043 |
|
l**********1
发帖数: 5204 | 7 plus
2005 Science review:
//www.ncbi.nlm.nih.gov/pubmed/16109872
and above review 1st author her lab:
//www.life.illinois.edu/clockworks/pages/Martha%20U.%20Gillette.html
and one Science 2011 paper shown fly sleep manipulation.
//www.ncbi.nlm.nih.gov/pubmed/21700877
lol |
|
l**********1
发帖数: 5204 | 8 细菌 贫营养的状态 若其lac operon进入 1/3 subharmonic oscillatory 状态的话 就是细菌的睡觉吧
具体参看下边的Goodwin BC 1965 paper
Introducing mutual coupling between the oscillators, with klz = 1.2, km = 0.
4, a phenomenon known as
subharmonic resonance is observed as shown in Fig. 9. Both variables now
have the same frequency, which is considerably smaller than either of the
free-running frequencies. The lower curve is Y~, and in it one can still see
the
original frequency on which has been superimposed the slower frequency of... 阅读全帖 |
|
l**********1
发帖数: 5204 | 9 Bingo
Zhuang XW 20 nm scale resolution by STORM never can across below
10 nm by color 4D Electron Microscopy for biological sample observation.
Caltech Science 2012 paper
Title:
Subparticle Ultrafast Spectrum Imaging in 4D Electron Microscopy
by
A Yurtsever et al.
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/22223801
detail figure please refer lower floor.
会phase out。
----
[回复]
[ 7 ]
发信人: sdogdog (｡◕‿@｡), 信区: Biology
标 题: Re: 超分辨光学显微镜生物应用有什么前景
发信站: BBS 未名空间站 (Fri Feb 1... 阅读全帖 |
|
l**********1
发帖数: 5204 | 10 please go to
//www.ncbi.nlm.nih.gov/pubmed/20228280
or
//www.ncbi.nlm.nih.gov/pubmed/18977028 |
|
l**********1
发帖数: 5204 | 11 Why only think that positive aspects from noise in biology 这个可以连接或
者引出好几个问题specificity, phenotypic
..
please go to
//www.ncbi.nlm.nih.gov/pubmed/21737735
//www.eng.cam.ac.uk/news/stories/2011/cellular_noise/
//www.ncbi.nlm.nih.gov/pubmed/20829788
..
？ |
|
l**********1
发帖数: 5204 | 12 Why only think that positive aspects from noise in biology 这个可以连接或
者引出好几个问题specificity, phenotypic
..
please go to
//www.ncbi.nlm.nih.gov/pubmed/21737735
//www.eng.cam.ac.uk/news/stories/2011/cellular_noise/
//www.ncbi.nlm.nih.gov/pubmed/20829788
..
？ |
|
l**********1
发帖数: 5204 | 13 please go to
//www.gwascentral.org/studies?
q=&t=ZERO&m=All&page=1&format=&page_size=50&o=Number+of+markers+(descending)
and
//www.ncbi.nlm.nih.gov/pubmed/19740415
or
//www.ncbi.nlm.nih.gov/pubmed/21158748 |
|
l**********1
发帖数: 5204 | 14 //www.ncbi.nlm.nih.gov/pubmed/17506643
//www.ncbi.nlm.nih.gov/pubmed/22307614
BAOZI two please. |
|
l**********1
发帖数: 5204 | 15 plus ftsQ
please go to
//www.ncbi.nlm.nih.gov/pubmed/21642457
Ps: how to get it?
Searching Pathway:
Bottom to Top:
//www.ncbi.nlm.nih.gov/pubmed/17098054
//www.springerlink.com/content/xl31814q3765h4g8/
//www.google.com
with key words:
then upper floor hint then upper floor of upper floor hint etc.. |
|
l**********1
发帖数: 5204 | 16 So how about TubZ
//www.ncbi.nlm.nih.gov/pmc/articles/PMC3132758/
C Moriscot - 2011
8 Jul 2011 – The phylum Crenarchaeota lacks the FtsZ cell division hallmark of bacteria ... cytokinesis and
budding of some enveloped viruses, little is known about the ...... Lowe J. Filament structure of bacterial
tubulin homologue TubZ.
//zh.wikipedia.org/wiki/泉古菌門
//www.ncbi.nlm.nih.gov/pubmed/20974911
//www.ncbi.nlm.nih.gov/pubmed/20818414
more below figure
or go to
http://www.mitbbs.com/article_t/Biology/31... 阅读全帖 |
|
l**********1
发帖数: 5204 | 17 cdvC or cdvA gene:
cdvC
Nitrosopumilus maritimusmmi
//www.ncbi.nlm.nih.gov/pubmed/21923770
cdvA:
Caldiarchaeum subterraneum
//www.ncbi.nlm.nih.gov/pubmed/21169198
details please refer
Swedish Uppsala Univ PhD thesis 2012:
full text link:
//uu.diva-portal.org/smash/record.jsf?pid=diva2:464718
its pp25
Yet another indication of essential
gene function comes from production of gene knockout mutated cell lines.
Attempts to delete the cdvC gene have not been successful (Hobel et al.,
2008). While neg... 阅读全帖 |
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l**********1
发帖数: 5204 | 18 //www.ncbi.nlm.nih.gov/pubmed/21050804
浮黴菌門
Isosphaera屬
//hamap.expasy.org/proteomes/ISOPI.html
Species: Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B)
Species code: ISOPI
Taxonomy: Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Isosphaera
(TaxID: 575540) [NEWT/ NCBI]
Description: Isosphaera pallida strain IS1B was isolated from a hot spring in Kah-nee-tah, Oregon, USA.
It is the only budding bacterium known to glide and the only heterotrophic bacteriu... 阅读全帖 |
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l**********1
发帖数: 5204 | 19 Exactly your opinion is right.
plus even without CryoEM below group still can do simulation with modeling for their most possbile
mechanism discovery
please refer the figure G its scale bar 200 nm it is below florescent microscopy resolution limits,
so they cannot resolve the fusion of the individual FIP3-endosomes.
cited from
//www.ncbi.nlm.nih.gov/pubmed/21486954
and
//www.ncbi.nlm.nih.gov/pubmed/16818889
will |
|
l**********1
发帖数: 5204 | 20 Single cell FRET
//www.ncbi.nlm.nih.gov/pubmed/22114669
//www.ncbi.nlm.nih.gov/pubmed/20535203 |
|
l**********1
发帖数: 5204 | 21 Proposal just:
Oxford nanopore based NGKOT Next Generation Knout Out Technique:
用某种 dsDNA topoisomerase single molecular level cut and relink dsDNA with
magnetic tweezers
NB: NGKOT 能BACbase 300KP 内任意地knoct out 比起20-35 kp Vector的片段内PCR knoct out还是效率高啊
最佳的是可以Knock in again within BACbase 300KP 内任意的 based Oxford nanopore platform.
References:
//www.ncbi.nlm.nih.gov/pubmed/21809210
//www.ncbi.nlm.nih.gov/pubmed/19377505
//en.wikipedia.org/wiki/Topoisomerase
//www.mitbbs.com/article_t1/Biology/31... 阅读全帖 |
|
l**********1
发帖数: 5204 | 22 不是Bio Science
而是Bioengineering吧
i.e:
制造 轻便蜘蛛丝生物材料 用于警/军用防弹衣的
please refer:
//www.ncbi.nlm.nih.gov/pubmed/22215590
or
//www.ncbi.nlm.nih.gov/pubmed/21458065
Antimicrobial functionalized genetically engineered spider silk
Biomaterials. 2011 Jun;32(18):4255-
---
or this one Bovine Stem Cell hamburg:
http://www.mitbbs.com/article_t/Biology/31637141.html
14F |
|
l**********1
发帖数: 5204 | 23 Please send to mail address
Goodwin BC.
Oscillatory behavior in enzymatic control processes. (1965)
Adv Enzyme Regul. ;3:425-
pubMed link:
//www.ncbi.nlm.nih.gov/pubmed/5861813
full text link:
//www.sciencedirect.com/science/article/pii/0065257165900671
Thank you so much in advance. |
|
|
l**********1
发帖数: 5204 | 25 if 尿激酶: by small-angle X-ray scattering paper:
//www.ncbi.nlm.nih.gov/pubmed/21669207
if 丝氨酸蛋白酶 you also hlod antibody of it,
then try by Surface Plasmon Resonance Analysis (SPR)
//www.ncbi.nlm.nih.gov/pubmed/19047064 |
|
l**********1
发帖数: 5204 | 26 if cysteine cathepsins
please go to:
//www.ncbi.nlm.nih.gov/pubmed/20860624
and
//www.ncbi.nlm.nih.gov/pubmed/9299326
ps: if you are satisfied with this answer,
BAOZI one please transfer to my mitbbs financial account,
Wei-bi 10. |
|
l**********1
发帖数: 5204 | 27 U sure?
even Yeast xxx better than non xxxx
Coelho MA, Gonçalves P, Sampaio JP.
Evidence for maintenance of sex determinants but not of sexual stages in red yeasts, a group of early
diverged basidiomycetes.
BMC Evol Biol. 2011 Aug 31;11:249
Abstract
BACKGROUND:
The red yeasts are an early diverged group of basidiomycetes comprising sexual and asexual species.
link:
//www.ncbi.nlm.nih.gov/pubmed/21880139
and
Pound GE, Doncaster CP, Cox SJ.
A lotka-volterra model of coexistence between a sexu... 阅读全帖 |
|
|
l**********1
发帖数: 5204 | 29 X-ray likes SXRF or 3D NMR for protein new 3d/4D structure/mechanism papers
sometimes can be allowed to use it.
i.e.:
//www.ncbi.nlm.nih.gov/pubmed/17712179
cited:
that we have examined by the X-ray fluorescence method in other ongoing studies (data not shown).
or
//www.ncbi.nlm.nih.gov/pubmed/17310249
cited:
>Measurements of 15N-backbone relaxation at 10 1C and analysis of data showed that, with the exception
of Lys738, all temperature-sensitive residues belong to flexible parts of the molecu... 阅读全帖 |
|
l**********1
发帖数: 5204 | 30 庄只是徐福炼丹 给秦始皇炼那个长生不老丹吧?
Xiaoliang Sunney Xie
Raman Scattering is King Route to 单分子Raman scattering 光 (possible)
1)
Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering
//apl.aip.org/resource/1/applab/v96/i21/p213701_s1
Abstract:
Raman spectra from single DNA molecules in their natural aqueous environment are presented. A DNA molecule that is anchored between two optically trapped dielectric beads is suspended in a solution with nanosized silver colloid particl... 阅读全帖 |
|
l**********1
发帖数: 5204 | 31 STORM Stochastic
is similar to below soft used in Stochastic Population Genetics:
LAMARC - Likelihood Analysis with Metropolis Algorithm using Random
Coalescence
//evolution.genetics.washington.edu/lamarc/index.html
俄罗斯外高加索人的百岁长寿因子的发掘的话 不会用这类super resolution microscopy的
relative papers:
1)
/www.ncbi.nlm.nih.gov/pubmed/16618935
2)
//www.ncbi.nlm.nih.gov/pubmed/19737113
3)
//info-centre.jenage.de/assets/pdfs/library/williams_EVOLUTION_1957.pdf |
|
|
l**********1
发帖数: 5204 | 33 有时候要看内容的
比如
JBC 280, 24371–24379.
by
Maruyama, M et al. (2005).
title:
Differential roles for sox15 and sox2 in transcriptional control in mouse
embryonic stem cells.
web link:
http://www.ncbi.nlm.nih.gov/pubmed/15863505
是以下炸药奖
Cell. 126: 663-76. 的敲门砖的话 (sox2 noted at Cell Abstract part)
JBC first author M氏and Cell 1st author T氏
今后找发考题的话 区别不大
当然前提是有Yamanaka 不错的 recommendation letter 的前提下
Takahashi K, Yamanaka S. (2006)
Cell. 126: 663-76.
Induction of pluripotent stem cells from mouse embryonic... 阅读全帖 |
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l**********1
发帖数: 5204 | 34 Please refer:
scanned Bessel beams in conjunction
with structured illumination and/or two-photon excitation to create thinner
light sheets (<0.5 μm) better suited to three-dimensional (3D) subcellular
imaging
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/21378978
Planchon TA, Gao L, et al.
Rapid three-dimensional isotropic imaging of living cells using Bessel beam
plane illumination.
Nat Methods. 2011 May;8(5):417-
>Abstract
>A key challenge when imaging living cells is how to noninvasively extract... 阅读全帖 |
|
l**********1
发帖数: 5204 | 35 Bingo
plus
Hochberg M.E. & Hawkins B.A.
Refuges as a predictor of parasitoid diversity. (1992)
Science 255:973-
//www.ncbi.nlm.nih.gov/pubmed/17793160
Hawkins B.A., Thomas M. & Hochberg M.E.
Refuge theory and classical biological control. (1993)
Science 262:1429-
//www.ncbi.nlm.nih.gov/pubmed/17736826
both were from
//mike.hochberg.free.fr/ |
|
l**********1
发帖数: 5204 | 36 给你介绍几个mentors 吧
ps: LZ 老板有一大堆CNS last/corresponding authorship records >=3 的话 以下请略去 不用看啦
瑞士 日内瓦大学的Prof. Jerzy Paszkowski
//www.unige.ch/sciences/biologie/plantsciences/grpaszkowski/Paszkowski_lab/
Members.html
his team Nature 2011 paper:
//www.ncbi.nlm.nih.gov/pubmed/21399627
和
法国克莱蒙费朗第一大学 Universite De Clermont - FerrandⅠ
Olivier Mathieu CR - CNRS
his Nature (2009) last/corresponding author paper:
//www.ncbi.nlm.nih.gov/pubmed/19734882
//www.gred-clermont.fr/index.php?option=com_content&tas... 阅读全帖 |
|
l**********1
发帖数: 5204 | 37 Re:
sunnyday
please send mail or Fax to
AP Sumiyama K. the corresponding author for
paper:
//www.ncbi.nlm.nih.gov/pubmed/20219670
ask for his help about your above questions.
his lab:
//sayer.lab.nig.ac.jp/~sumiyama/index-e.html
or
//www.nig.ac.jp/section/saitou/saitou-e.html
or
//sayer.lab.nig.ac.jp/index-e.html
You can also ask him for sending this one:
pT2AL200R150G
by Fedex
of course you told him your US side Fedex receive account number and your side pays
all transportation charge.
... 阅读全帖 |
|
l**********1
发帖数: 5204 | 38 The structure and expression of the [alpha]2 tubulin gene in Drosophila melanogaster. ... of the [alpha]2
tubulin gene in Drosophila melanogaster Author: Jieying Bo Publisher: 1989. ... Dissertation: Thesis (Ph. D.)-
-Brandeis University, 1989.
link:
//www.worldcat.org/title/structure-and-expression-of-the-alpha2-tubulin-gene
-in-drosophila-melanogaster/oclc/20880213
her 1986 PNAS paper link:
//www.ncbi.nlm.nih.gov/pubmed/3095837
(NB: 3rd author)
another her paper:
//www.ncbi.nlm.nih.gov/pubmed/... 阅读全帖 |
|
b*****n
发帖数: 1841 | 39 1.The promoter region of the Drosophila alpha 2-tubulin gene directs
testicular and neural specific expression.
Bo J, Wensink PC.
Development. 1989 Jul;106(3):581-7.
PMID:2513179[PubMed - indexed for MEDLINE] Free Article
Related citations
2.Tissue-specific and constitutive alpha-tubulin genes of Drosophila
melanogaster code for structurally distinct proteins.
Theurkauf WE, Baum H, Bo J, Wensink PC.
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8477-81.
PMID:3095837[PubMed - indexed for MEDLINE] Fre... 阅读全帖 |
|
l**********1
发帖数: 5204 | 40 alternatively you can try Oligotex-dt30 mRNA purification kit for total RNA
sample:
Manual book:
//catalog.takara-bio.co.jp/PDFFiles/NTrap%20mRNA_j.pdf
Vender link:
//www.mn-net.com/Products/DNAandRNApurification/RNA/NucleoTrapmRNA/tabid/1328/language/en-
US/Default.aspx
Reference:
please go to
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/18678863
T Yabu - 2008 JBC
31 Oct 2008 – In zebrafish embryonic cells, the endogenous SMase enzyme was
.... using the Oligotex-dt30 mRNA purification kit (Tak... 阅读全帖 |
|
l**********1
发帖数: 5204 | 41 SILAC (Stable isotopic atoms for quantitative
mass spectrometry analysis)
Plus spatial proteomics eGFP/alternative biomarker labelling
details
please go to
original paper link:
//www.ncbi.nlm.nih.gov/pubmed/21937730
or
2011 one review:
//www.ncbi.nlm.nih.gov/pubmed/21476986
To perform a system-wide analysis of
protein turnover
In human proteome:
cultured human cells. Protein abundance and
the rates of protein synthesis, degradation and turnover have
been measured in parallel for whole cells and... 阅读全帖 |
|
l**********1
发帖数: 5204 | 42 Continue again:
YFP/GFP/Luciferase etc bioluminescence marker methods
for measurement of clearance rate constants within living cells
please refer
Science 2011 paper (YFP):
//www.ncbi.nlm.nih.gov/pubmed/21233346
and 2012 paper (GFP) :
//www.ncbi.nlm.nih.gov/pubmed/22499809
Ps:
Below both figures were from 2012 Science paper its Supplementary Materials document,
web link:
//www.sciencemag.org/content/early/2012/04/11/science.1221920/suppl/DC1
Good Luck for your CNS revised version submission. |
|
l**********1
发帖数: 5204 | 43 latin 学名的话
for example:
草履虫: Paramecium caudatum
//en.wikipedia.org/wiki/Paramecium_caudatum
你想咋念就咋念 英美人Bio PI中 会看懂拉丁语古罗马文全文的不会超过3%
又不是 夺宝奇兵 里的 Junior Indiana Jones
//en.wikipedia.org/wiki/Indiana_Jones
Ps: 正好撞到以下的两篇论文 谈到那种草履虫
//www.ncbi.nlm.nih.gov/pubmed/21627782
//www.ncbi.nlm.nih.gov/pubmed/22179582 |
|
l**********1
发帖数: 5204 | 44 用David
//david.abcc.ncifcrf.gov/conversion.jsp
把Drosophila, 几个candidate genes Illumina probeID 转换成 Affymetrix ID
then try piRNA-producing transcripts
with Affymetrix gene expression data
FTP//ftp.aarnet.edu.au/pub/bioconductor/packages/2.8/Drosophila_melanogaster.html
Reference link:
//www.ncbi.nlm.nih.gov/pubmed/20022248
key description:
>
Selective Production of 3’-UTR-Directed piRNAs
from Gonadal mRNAs
We next assayed the relationship of piRNA-producing transcripts
with Affymetrix gene exp... 阅读全帖 |
|
l**********1
发帖数: 5204 | 45 please try direct micro injection donor mtDNA to your target organism.
please refer
Takeda K et al
Influence of Intergeneric/interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine
Oocytes after Injection of Mitochondria Derived from Somatic Cells. (2012)
J Reprod Dev. 2012 Mar 9. [Epub ahead of print]
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/22447326
Ps:
two us mentors of this aspect in Auburn University
College of Veterinary Medicine
Auburn, Alabama
a) MICHAEL H. IRWIN... 阅读全帖 |
|
l**********1
发帖数: 5204 | 46 Bingo
it was from
更新时间： 2001-June-12
在美国当教授的北大毕业生(部分)
//www.gotopku.cn/data/detail.php?id=1974
----
Recent 2007 database please try:
[八卦江湖] 带大家认识一下顶尖的美国华裔/华侨女科学家们（有PP有真相）
作者：第五哇 发表日期：2010-8-19
//www.tianya.cn/publicforum/content/funinfo/1/2195431.shtml
---
only HHMI PI please go to
//bbs.netbig.com/thread-2113463-1-1.html
发表于 2011-11-18
NB: updated 2012-May-13
著名医学研究HHMI华裔/华侨PI科学家汇总
华裔/华侨 医学研究 HHMI, 科学家, 著名
Howard Hughes Medical Institute （HHMI） 是全球规模最大的非盈利性私立医学研
究所-基金会，拥资120忆，为美国第二大的慈善机构，仅次... 阅读全帖 |
|
l**********1
发帖数: 5204 | 47 Continue to lower floor:
U Tube:
//www.youtube.com/watch?v=5jJ_BAGV__E
and one Nature 2011 paper link:
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/21938060 |
|
l**********1
发帖数: 5204 | 48 UCSF Vice Executive Vice Chancellor
Jeffrey A. Bluestone
//www.diabetes.ucsf.edu/members/jeffrey-bluestone
//www.ncbi.nlm.nih.gov/pubmed/20432533
//www.ncbi.nlm.nih.gov/pubmed?term=%22Bluestone%20JA%22%5BAuthor%5D |
|
|
l**********1
发帖数: 5204 | 50 Jian-kang Zhu (强哥)
//www.ncbi.nlm.nih.gov/pubmed?term=jian-kang%20zhu
without any co-authorship paper with Chua (冯敬荛)
Philip N. Benfey (丁力)
//www.ncbi.nlm.nih.gov/pubmed?term=Philip%20N.%20Benfey
had co-authorship papers records with Chua |
|
