n********g
发帖数: 6504 | 1 RAD啊。捂着不怕变馊啊。一个萝卜一个坑，不赶紧填过了村就没店了。只要填了坑，
虽然拿不到34w，组织上肯定也会照顾你的，免费手纸，水果，饮料，洗澡水啥的随便
猥琐。多干几年的MacBook Pro攒了几本要不嫌碍事没还也没人有意见。 |
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b********n
发帖数: 38600 | 2 Stories: Trump’s Tariffs Betray Base
https://terribletariffs.com/stories/
Then there are the countless farmers and exporters who have seen their
markets dry up as trading partners impose retaliatory tariffs.
'47 Brand Hats: The Massachusetts company, which faces higher raw material
costs thanks to Trump's tariffs, cannot find domestic suppliers to produce
hats. It is considering job cuts to compensate.
5th Avenue Energy: This small California renewable energy firm found that
lighting fixtures an... 阅读全帖 |
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发帖数: 1 | 4 快过节了，各个仪器试剂厂商都来送零食，你老这么拿可不像话 |
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发帖数: 1 | 5 藏液氮里了，永久包存
：快过节了，各个仪器试剂厂商都来送零食，你老这么拿可不像话 |
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发帖数: 1 | 7 我知道你说的是啥，不就是Bio-Rad 那种跑磁珠子的那种机器吗，还不能取代western
blot |
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发帖数: 1 | 8 Bio-Rad微滴式数字PCR技术（ddPCR）是第三代PCR技术，无需标准参照物质即可给出
样本的拷贝数浓度而非Ct值，其检测结果为判读提供量化的依据。根据LOB(limit of
blank)和未知样本的定量结果作出判读，可避免人为错误，缩小灰区，提高检出率。
ddPCR独有的微滴处理技术，使其对PCR扩增效率的变化不再敏感，不惧含有PCR抑制物
样本的挑战。随着疫情的变化以及流行病学调研的推进，更多类型的检测乃至环境监测
也可能需要进行，ddPCR的这一优势会发挥作用。此外，如果新型冠状病毒发生变异，
病毒基因序列上引物、探针靶向位置出现SNV，ddPCR亦能克服引物、探针结合能力下降
带来的扩增效率变化，确保稳定检出。不仅如此，还能根据样本微滴荧光信号的变化，
发现引物探针靶标区域可能出现变异的病例，为NGS测序分析病毒变异提供样本，助力
流行病学研究。 |
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发帖数: 1 | 9 刘鹤副总理不是刚签了2000亿美元的单子吗，撒开了买Bio-Rad,这才叫钱花到刀刃上，
而且ddPCR很可能能帮助找出那些没有症状却带毒的新冠“伤寒玛丽” |
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发帖数: 1 | 10 ddPCR 没有问题的，放心吧，只有假阳性，没有假阴性
Digital PCR as a tool to measure HIV persistence
Although antiretroviral therapy is able to suppress HIV replication in
infected patients, the virus persists and rebounds when treatment is stopped
. In order to find a cure that can eradicate the latent reservoir, one must
be able to quantify the persisting virus. Traditionally, HIV persistence
studies have used real-time PCR (qPCR) to measure the viral reservoir
represented by HIV DNA and RNA. Most recently, digital P... 阅读全帖 |
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发帖数: 1 | 11 这个属于奇技淫巧，
不如Bio-Rad可以马上大规模部署展开 |
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a*****o
发帖数: 209 | 12 看图：
http://atmc.jp/plant/rad/?n=1
Radiation monitoring charts published by the Japanese government show
that the radiation levels at Fukushima reactor number 1 have spiked to 100
sieverts per hour. It should be noted that it actual radiation could
actually be higher because 100 sieverts per hour is the maximum that can be
displayed on the chart.
Exposure to this level of radiation will cause immediate death.
According to the NIH radiation levels of 4 sieverts per hour will cause
fatality... 阅读全帖 |
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D**S
发帖数: 24887 | 13 【 以下文字转载自 Queer 俱乐部 】
发信人: DSJS (DSJS), 信区: Queer
标 题: 为什么会有gay repulican这种东西
发信站: BBS 未名空间站 (Thu Mar 11 22:39:38 2010, 美东)
而且还弄出那些恶心人的事情来。
随便一说，就有Larry Craig, Mark Foley，那个什么教会的Ted Haggard估计也是GOP
的。这不，最近又来了个Roy Ashburn。一个gay republican (of course closeted
until now)，而且多年以来“推动”反同法案。实在是罪可诛。
http://articles.latimes.com/2010/mar/09/local/la-me-ashburn9-2010mar09
State Sen. Roy Ashburn says he's homosexual
Days after his DUI arrest, the Republican lawmaker makes the declaration to
a Bakersfield rad |
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m******8
发帖数: 2153 | 14 http://emf.mercola.com/sites/emf/archive/2011/03/17/vatican-rad
Children in the area (Vatican) were six times more likely to develop
leukemia.主教才是真正的未来花朵的杀人黑手
The 300-page report prepared by Italy's most prestigious cancer research
hospital called the connection between Vatican antennas and childhood cancer
"coherent and significant." |
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c**i
发帖数: 6973 | 15 李大同, 点评中国: 谣言止于公信. BBC Chinese, July 27, 2009.
http://news.bbc.co.uk/chinese/simp/hi/newsid_8170000/newsid_8170700/8170799.stm
My comment:
(a) The commentary started with “我们小时候都读过《咕咚来了》的童话.”
(b) Cobalt-60 creates beta decay. Beta particles can be stopped by a sheet
of aluminum that is 2 to 3 mm thick. Perhaps because the author, Mr. 李大同,
was starved of information--Chinese government has said little about the
incident—his commentary states, “6月14日15时，辐照室内接受辐照加工的辣椒粉
由于放射源的长时间照射，温度过高自燃。” Beta rad |
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l****z
发帖数: 29846 | 16 By James Lewis
Hi Tech Lynching is here again! Shades of Justice Clarence Thomas and Anita
Hill. They're going to play the same sadistic power game they lost with
Justice Thomas. Well, let 'em try, but now they are facing a revived
conservative media. This is a trial of strength. When Clarence Thomas was
nominated to the Supreme Court he had few defenders. Today we have talk
radio and the blogs.
As a morality play this looks like Snidely Whiplash against Little Nell. The
Corporate Media are wide... 阅读全帖 |
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k****g
发帖数: 1509 | 17 Is that true? Which radio? I got satellite rad in my car. |
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k***n
发帖数: 997 | 19 it's sealed in the thermostat housing on your engine.
to get there you need firstly drain coolant, disconnect several hoses
including upper rad hose on the flange side, probably smog pump hose, some
specially shaped thingy.
might also have to remove air box, battery first too.
it's necessary to have a factory repair manual and tools like hose clamp
pliers，tweezers, offset wrench.
overheating的帖子很多，一般是thermostat jammed closed, or does not open
progressively as coolant temp increases when more cool... 阅读全帖 |
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h****y
发帖数: 9234 | 20 sin(10 degree)= 0.1736
将近0.2g的加速度的分量了还是很大的.
你是不是把10/180*pi然后放进去结果还是用的度而不是rad... |
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b***6
发帖数: 6011 | 21 head gasket 漏的话有很多症状，但是不一定都表现出来
机油颜色正常吗？milky？
打开rad cap 看看液面正常吗？ |
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B*N
发帖数: 455 | 22 http://newyork.craigslist.org/brk/cto/3884192184.html 链接在这里
不想买日本车，请大家看看这个车砍价到多少钱合适，谢谢。卖家要价9700
This Grand Caravan powered by a 3.3 liter V6 engine !!! DRIVER and PASSENGER
CLIMATE CONTROL!!! AUX INPUT !!! CRUISE CONTROL !!! THREE ROW SEATS!!!
MEDIUM SLATE SEATS !!! HEATED MIRRORS !!!
This car has a 101k miles on it. Its automatic transmission shifts very
smooth. The paint looks great and is very glossy like new. This Grand
Caravan runs excellent and is very responsive. This Grand Caravan is loa... 阅读全帖 |
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m****n
发帖数: 23 | 23 事故story：上个月租车在加州一条State route上行驶，快到一路口（无红绿灯）时一
辆车忽然从垂直的那条路上左拐进来，我前面的A车急刹车停住，而我来不及刹住还是
撞到A的后面，我的前面bumper和他的后面bumper损坏。警察过来后觉得没大事，没有
留下report。
关于保险：没有买租车公司的保险，而是第三方Allianz公司的Collision/Loss Damage
Insurance，用来cover所租的车。
现在问题来了，租车公司给我和保险公司的账单显示：
4k: Physical Damage
200: Loss of Use (5.16 days at $41.57)*
150: Administrative Fee
1.5k: Diminution of Value
40: DV Appraisal Fee
Total Claim Amount接近6k
但保险公司给租车公司说他们不cover其中diminiution of value那部分，就是车的价
值折损费。租车公司委托一家Hybrid Claimg Group公司做的diminiution value... 阅读全帖 |
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F******0
发帖数: 44 | 24 CREME 3.5ML
SOFT CREME 3.5ML
GEL CREME 3.5ML
CONCENTRATE 3ML
REG/RAD/LIFTING SERUM 3ML
每种各几千个，日期都是13年下半年到14年初，有兴趣的EMAIL联系，国内交易！谢谢
D******[email protected] |
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b**********i
发帖数: 1482 | 25 SECURITY AND
SUPPORT
• Symantec Norton Internet Security™ 2010 60-day Trial(15)(19b)
• HP Support Assistant
• HP PC Recovery
• HP Advisor
• HP ProtectSmart Hard Drive Protection
PRODUCTIVITY AND
FINANCE
• Microsoft® Works
• Microsoft® Office 2007 Home & Student 60-day Trial(19)
• Microsoft® Silverlight
• Sun® Java
MULTIMEDIA • eBay®
• HP MediaSmart
• HP Webcam
• Pandora® Internet Rad |
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h*****d
发帖数: 210 | 26 EMSA 新手，希望能碰到生物学的老师帮我看看。
我从种子提nuclear extract, 想做EMSA, 用的是DIG-Gel shift kit. 开啥有了
TATABOX （5 repeats) 25bp in length, 试试看蛋白是不是有功能的，结果没加蛋白
的free probe也有带，所以认为我那加了蛋白后产生的带不是真的binding，因为2 带
的位置是一样的。但是有些情况用其他大一点的探针，50bp, 会得到smear. 还有我跑
电泳的胶很小，8x8cm（bio-rad)， 5% native gel. 0.25x TBE buffer, 4C, 80V.
问题：
1）怎么能证明蛋白是用功能的？现在没有binding， 不知是不是蛋白的原因，SDS-
PAGE 检测蛋白还行。抽的几次都是结果差不多，尽管有些带很强且集中在2个区段，但
总体来讲，蛋白的分布大小都有。
2)能不能找个其他probe 做postitive control。 有人可以推见一下啥探针？
3）我是用的nuclear extract, 探针可不可以用200bp 长的。
4)调整些啥才能有好 |
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b****o
发帖数: 806 | 27 我在NJ，想买BIO-RAD的产品，但是他们不肯卖给我，需要提供Lab的详细资料，我没有
，哪位老大能帮我代买一下？定有酬谢 |
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L***s
发帖数: 9258 | 29 发信人: Mang (忙 盲 茫), 信区: TsinghuaCent
标 题: 任教于美国一流大学的部分清华学子
发信站: 水木社区 (Tue Sep 10 11:30:55 2013), 站内
【致谢】感谢参与编写和提供信息的以下校友：
IhateBS strong
根据美国US News 2013年美国大学排名前100的顺序统计，缺漏错误之处难免，请大家
补充
分为Full Professor（正教授）、Associate Professor（副教授）和Assistant
Professor三类，
其中前两类一般具有终身教职，不含解放前的清华学子，也含有极少数非tenure track
教职
人员,和已离职的人员，只列出，不计入统计总数。
共有在职Tenure track系列教职的共492 人，其中90名正教授。副教授186人
另有部分著名实验室全职研究人员22人
----------------------------------------------------------------------------
1,哈佛大学 6
林希虹
Professor, 生物统计系... 阅读全帖 |
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f****y
发帖数: 737 | 30 请大家想一想，英语是谁发明的？英国人呗！
英国人认不认识汉语？不认识！
那么英国人在学英语单词的时候需不需要记住单词的汉语意思？不需要，英国人的英语
课本里根本就没有汉字，何谈记住单词的汉语意思？
那么既然英国人学英语不需要记住(甚至根本就见不到)单词的汉语意思，那么中国人学
英语为什么要去记住单词的汉语意思呢？这种做法大家不觉得奇怪吗？
然而由于中国人学英语时都在背单词的汉语意思，因此大家反而觉不出“背汉字”有什
么奇怪的了。
其实仔细想一想，这个行为真的很奇怪，奇怪的根源不在于行为本身，而在于中国人普
遍不会直接识别英语单词的意思，因而只好靠汉语符号来机械地帮助记忆英语单词的意
思，这样去学英语不仅多此一举，而且必然会陷入苦海无边的符号记忆灾难中。
其实英语单词和汉字一样，存在着很多的“偏旁部首”，知道了偏旁部首你就可以根据
它们直接来猜测单词的意思，虽不说百分之百猜准，但起码可以猜测个大概，至少在别
人告诉过你单词的意思后你可以恍然大悟地领会它，这样就可以大大增强你对英语单词
“见字识意”的能力，做到真正认识一个单词，而把它的汉语意思仅做为一般参考。
举几个例子来说吧：
比如单词... 阅读全帖 |
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t********1
发帖数: 18 | 31 买房后发现自己condo的楼顶上有两个Cell phone antenna。听说每年condo associate
能收$20,000，相应homeowner的condo fee每月少~$50。
刚开始搬进来的时候我就觉得有点不对，有时感觉耳边有响声、晚上睡不着，或半夜总
醒，有时又感觉很困。我开始以为刚换了个新地方，还没习惯过来。但这种情况一直持
续，没见太大变化，我以为是自己工作压力大的原因，就尽量调节来自工作的压力。又
过了一段时间，有时觉得头疼。这所有的感觉都是以前没有的，至少没有长时间持续有
这些症状。最近发现楼顶上有两个Cell phone antenna。上网一查吓了大跳，长期可能
引发Cancer，后面有些链接。
我给我们condo的manager打电话，他很显然不愿意跟我谈搬走这两个antennas。
Trustee的人都不住这儿，而且这里大多是租客，大部分homeowner都不住这里。请问各
位有什么好建议，怎么能把这antennas弄走？这种情况我该怎么处理？
另外，不知这种把cell phone antenna装在居民楼顶是否违法？有没有理由sue 电信公
司或... 阅读全帖 |
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v****o
发帖数: 978 | 33 固定收益部分:
PGH, ERF, DUK, JNK, USHYX, DPFFX，INTC
资源部分:
SLV,MOS,USAGX,MCP,GDX
中国部分：
CEA,MCHFX
长期赌博部分:
MJNA, RAD
最关键的倒是以下：
1、祝愿家人朋友健康，小朋友出世平安
2、祝愿自己身体健康， 每星期至少跑10英里
3、祝愿自己事业有所突破
4、祝愿本班广大网友心想事成
愿南无阿弥陀佛大慈大悲观世音菩萨保佑！ |
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m*****n
发帖数: 5245 | 35
Used to, not sure about this year |
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p******y
发帖数: 50 | 37 Internet Engineer / Application Developer
Strong in J2EE, Spring, Hibernate, SQL
Benefits: 免费医疗，年末盈利共享，工作时间灵活， 工资 8W 左右
Location: Keller, TX
Details:
Internet Engineer / Application Developer
Category: Domestic
Type: Employee
Description:
The Internet Engineer/Application Developer is responsible for developing,
maintaining and supporting multiple new and existing external and internal
facing web applications with many dynamic modules. The Internet Engineer/
Application Developer... 阅读全帖 |
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t********3
发帖数: 36 | 38 Location: Las Vegas,vada
Must Have Skills: Object-Oriented PHP, My SQL, JavaScript, AJAX, Perl, (
X)HTML, CSS, Web services/SOAP/XML, Strong Communicator, Web based design
patterns
Desired Skills: SVN configuration management, Ant, Bash, Unix, XMLRPC,
exposure to RAD, Agile, Ruby-on-Rails
Good to have: Familiar with web & email marketing, online advertisers
and publisher terminologies (CPC, CPA, CPC, CPM, mailing lists, affiliates..
.etc)
Job Roles / Responsib... 阅读全帖 |
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d*********n
发帖数: 52 | 39 My team has an immediate opening now, I will be reviewing your resume and
conduct a phone interview with you directly if I find you qualified.
Required skills:
Advanced Skills in Object-Oriented Javascrip,
Experience implementing AJAX/REST(like) API's,
Experience with JavaScript code structure, delay loading and execution
optimization,
Strong experience in JSP, DOM, JSON, XML parsing and other data transport
protocols,
Hands-On experience developing Core Java Framework using patterns,
Experience... 阅读全帖 |
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d*********n
发帖数: 52 | 40 My team has an immediate opening now, I will be reviewing your resume and
conduct a phone interview with you directly if I find you qualified.
Required skills:
Advanced Skills in Object-Oriented Javascrip,
Experience implementing AJAX/REST(like) API's,
Experience with JavaScript code structure, delay loading and execution
optimization,
Strong experience in JSP, DOM, JSON, XML parsing and other data transport
protocols,
Hands-On experience developing Core Java Framework using patterns,
Experience... 阅读全帖 |
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d*********n
发帖数: 52 | 41 My team has several immediate openings now. For e-commerce project and front
-end web application development. Please send me your resume for
screening.
We do H1-B and Green card. Prefer local candidates.
Required skills:
Advanced Skills in Object-Oriented Javascrip,
Experience implementing AJAX/REST(like) API's,
Experience with JavaScript code structure, delay loading and execution
optimization,
Strong experience in JSP, DOM, JSON, XML parsing and other data transport
protocols,
Hands-On experi... 阅读全帖 |
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s******e
发帖数: 493 | 42 1.
swing for desktop apps. javafx for ria (kind of too late for the gamesupport
. dying like silverlight). on the other side, some server side gui techs
like jsf (swing at server side), gwt, vaadin , etc have their own markets
2. java was never designed for competing the speed with C++. it and its
extension (j2ee) with all powerful open source frameworks are for really
helpful for RAD (rapid application development), which is its cliche.
3. considering c# is native to windows, it might be a good... 阅读全帖 |
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d*********n
发帖数: 52 | 43 I have three dev position openings now. It needs oracle database, java
object, servlet, ejb, javascript, jsp knowledge. Please send me your resume,
if i see a fit for my team, I will be in touch with you.
We do H1-B and Green card. Prefer local candidates, since we will not be
able to sponsor the relocation/airplane tickets for the in-person interview.
Required skills:
Advanced Skills in Object-Oriented Javascript,
Experience implementing AJAX/REST(like) API's,
Experience with JavaScript code st... 阅读全帖 |
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n*********l
发帖数: 52 | 44 有意者，请火速站内联系。倾尽全力帮助同胞 (特别是刚毕业或转行的朋友)!!! 谢谢
Must be able to pass a background investigation to obtain a security badge
to enter the applicable government facility.
不好意思, 必须公民或绿卡
Technical Skill Requirements:
• Must have thorough understanding of all aspects of the software
development life cycle (SDLC).
• Must be able to elicit business requirements from business users,
develop functional requirements based on the business requirements and
produce detailed requirements documenta... 阅读全帖 |
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N**C
发帖数: 3 | 45 The world's premier provider of investment research, market indices, credit
ratings, financial data, and fixed income research and analysis. With more
than 10,000 employees and offices in nearly two-dozen countries, the
employer is valued by investors and financial decision-makers everywhere for
its analytical independence, market expertise and thought leadership. For
150 years this company has been an integral part of the global economic
infrastructure. Its operations provide essential informat... 阅读全帖 |
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f***e
发帖数: 2 | 46 Software Developer (C++,C#,Delphi - Advanced TV analysis tools)
TechEdge is an innovative and specialised software house, and we develop
advanced analysis and optimization software to major TV broadcasters and
Media Agencies across the World.
We are experiencing major growth in our business and seek to expand our
software development team with several talented individuals.
The position:
We are looking for a talented and passionate Software Developer. You will be
an integral part of a serious and... 阅读全帖 |
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n*********l
发帖数: 52 | 47 有意者，请火速站内联系。倾尽全力帮助同胞 (特别是刚毕业或转行的朋友)!!! 谢谢
Must be able to pass a background investigation to obtain a security badge
to enter the applicable government facility.
不好意思, 必须公民或绿卡
Technical Skill Requirements:
• Must have thorough understanding of all aspects of the software
development life cycle (SDLC).
• Must be able to elicit business requirements from business users,
develop functional requirements based on the business requirements and
produce detailed requirements documenta... 阅读全帖 |
|
t********1
发帖数: 18 | 48 买房后发现自己condo的楼顶上有两个Cell phone antenna。听说每年condo associate
能收$20,000，相应homeowner的condo fee每月少~$50。
刚开始搬进来的时候我就觉得有点不对，有时感觉耳边有响声、晚上睡不着，或半夜总
醒，有时又感觉很困。我开始以为刚换了个新地方，还没习惯过来。但这种情况一直持
续，没见太大变化，我以为是自己工作压力大的原因，就尽量调节来自工作的压力。又
过了一段时间，有时觉得头疼。这所有的感觉都是以前没有的，至少没有长时间持续有
这些症状。最近发现楼顶上有两个Cell phone antenna。上网一查吓了大跳，长期可能
引发Cancer，后面有些链接。
我给我们condo的manager打电话，他很显然不愿意跟我谈搬走这两个antennas。
Trustee的人都不住这儿，而且这里大多是租客，大部分homeowner都不住这里。请问各
位有什么好建议，怎么能把这antennas弄走？这种情况我该怎么处理？
另外，不知这种把cell phone antenna装在居民楼顶是否违法？有没有理由sue 电信公
司或... 阅读全帖 |
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z**k
发帖数: 945 | 49
本文是根据我们几个月买房的经验心得收集而成， 收集了一般买房中需要注意的事项
， focus on Richmond, VA, 希望对大家提供些许帮助。
General Rule: LOCATION, LOCATION, LOCATION, Be mindful about the location of
the house you buy. It is the single most important investment most people
make in their life. Using an investment perspective. Do not buy any one that
you think it is good but may never get sold or rented. Easy to sell, easy
to rent out, easy to liquidate should always be at your mind when you buying
a house. Consider the community you will be... 阅读全帖 |
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