Y*******i
发帖数: 2299 | 1 好像国内的期刊，作者自己都没有免费版，只有带水印的。好多学校都没有这小期刊
Journal of biomaterials and tissue engineering.
4 12 December 2014
Title: Biomimetic Double-Layered Scaffolds Composed of Jellyfish Collagen
and Chitosan for Cartilage Tissue Engineering
[email protected]
/* */
谢谢！ |
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w********h
发帖数: 12367 | 2 tissue engineering需要体内植入scaffold材料阿。。。 |
|
s******y
发帖数: 28562 | 3 scaffold材料一般都会在表面裹上厚厚的matrix 吧。如果光是用个硬塑料的话大部分
细胞根本就不乐意往上长，而且就算长了也会被诱导成错误的细胞吧。 |
|
s******y
发帖数: 28562 | 4 我怎么记得大部分成功的scaffold 其实就是一个掏空细胞了的extracellular matrix?
你是说直接扔个塑料模子进去也行？ |
|
w******e
发帖数: 1187 | 5 我的email: [email protected]
/* */ 非常感谢！！
paper 1:
title: Multi-Specific Antibodies for Cancer Immunotherapy
BioDrugs, August 2014, Volume 28, Issue 4, pp 331-343
Ron D. Jachimowicz, Sven Borchmann, Achim Rothe
paper 2:
title: New Horizons in Therapeutic Antibody Discovery: Opportunities and
Challenges versus Small-Molecule Therapeutics
J Biomol Screen April 2015 20: 437-453
paper 3:
title: Non-immunoglobulin scaffolds: a focus on their targets
Katja Škrlec, Borut Štrukelj, Ale#... 阅读全帖 |
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O**********a
发帖数: 317 | 6 我现在有Cas9的老鼠，想敲掉一个基因，用HDR模版引入点突变。准备直接注射卵；因
为有Cas9老鼠，所以只需要注射gRNA＋HDR template。
设计好了gRNA，是必须构建载体用T7加酶体外转录获得吗？
为了简便，我考虑直接合成gRNA＋scaffold RNA。加起来有104 mer，是不是不可行？
这个长度的RNA能直接合成吗？是不是会很贵？
非常感谢！ |
|
c***y
发帖数: 615 | 7 假设genome scaffold 长度 5 million bases， 一段 长度为20 的特定序列理论上可
以出现多少次?
Thanks a lot! |
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S******i
发帖数: 71 | 8 应该把自己的出生告诉大家，不要空对空，要留下学校的阳西地址，还有最好把系主任
的联系方式也放这里
Guo Huang Ph.D.
Assistant Professor
CVRI Investigator
Website
Research Interests:
Comparative study of heart development and regeneration, ischemic heart
diseases, stem cell, cardiomyocyte proliferation, regenerative biology
Program Areas
Vascular Biology and Atherothrombosis
Developmental Biology and Congenital Anomalies
Channels and Arrhythmias
Muscle Biology and Heart Failure
Prediction and Prevention of Cardiovascular Disease
Research Summary
... 阅读全帖 |
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v********a
发帖数: 646 | 9 面对这个1979年的单身女郎，从中专毕业，到参加成人自考拿到同等学历，然后一路逆
袭，最后傲然在苏州大学就职。
只有两个字：佩服
http://hlxy.suda.edu.cn/jiaoxuekeyan/yanjiushengdaoshiminglu/20
王丽，女，教授，硕士研究生导师
研究方向
慢性病运动康复：重症病者包括心脑血管疾病、骨关节系统疾病和终末期肾病等患者的
运动康复。
体能与疾病：体能水平与疾病发生和预后之间的关系；
社区康复：糖尿病、骨质疏松等的社区康复护理干预。
联系方式
电话：0512-65221499 (办公室); 138-1486-1702 (手机)
邮a class="__cf_email__" href="/cdn-cgi/l/email-protection" data-cfemail="3dd99e875154104a5c535a100c7d4e48595c13585948135e53">[email protected]/* */; [email protected]/* */
地址：苏州市十梓街1号苏州大学护理学院本部20... 阅读全帖 |
|
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r*******e
发帖数: 123 | 11 请发到我的邮箱: [email protected]/* */
万分感谢
Actin Cytoskeleton-Mediated Constriction of Membrane Organelles via
Endoplasmic Reticulum Scaffolding
Carol Lynn Curchoe and Uri Manor*
Waitt Advanced Biophotonics Center, The Salk Institute for Biological
Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, United
States
ACS Biomater. Sci. Eng., Article ASAP
DOI: 10.1021/acsbiomaterials.6b00802
Publication Date (Web): February 9, 2017 |
|
s******c
发帖数: 331 | 12 现在难得看到这么激烈的有关生物学的讨论了！
支持这个答主一下。不过结构生物学的意义多数还是很基础科学的，另外一方面，所谓
biomedical research大多数也是这样的，研究一些蛋白在疾病中的作用是现在NIH很喜
欢的，但是实际上多数的蛋白质并不能作为药物靶点。还有，结构是功能的基础是没错
的，但是知道结构对理解功能有多大的作用，就是case by case的事情。各种protease
enzyme的结构对于功能的研究显然是critical的，不然根本不知道为什么类似的蛋白
可以有不一样的底物选择性和不同的催化活性，这方面指导药物开发的例子很多，比如
HIV的各种逆转录酶的基于结构的药物设计，一些超大分子，比如叶绿体，呼吸链复合
物，yigong的splicesome，结构对于功能的理解是决定性的，比如结构出来之后大家才
知道呼吸链里电子是怎么传递的，各种小分子是怎么结合的。至于在细胞内做scaffold
的很多蛋白，结构对于功能的意义在我看起来比较的弱，同样还有各种跟免疫相关的比
如BCR TCR，精细的结构信息可能对功能指导意义没有很强。这方面看看结构生物学本
身的侧重点其... 阅读全帖 |
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V******1
发帖数: 8 | 13 作为一个没做过膜蛋白的伪structure biologist，赞一下专业回答。
再补充一点生物大分子的。结构可能对于大分子比如抗体的指导作用没有receptor/
ligand或者enzyme/substrate那么看起来那么直接，但也有很大帮助。比如知道target
的结构，就可以有针对性地display更感兴趣的epitope，尤其是那些non-linear的
epitope单单根据序列是没法预测的。反过来说也可以突变掉unwanted epitope来避免
screen到不想要的抗体。拿到antibody/antigen complex的结构，可以根据结构做有针
对性的affinity maturation来进一步优化抗体。design bi-specific甚至tri-
specific antibody，需要考虑几个target epitope在空间上的位置。做vaccine的，可
以根据结构来design engineer的antigen，使之更有效地产生neutralizing antibody
。还有找到一个好的抗体，patent的时候也得知道epitope的不是，虽... 阅读全帖 |
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w********h
发帖数: 12367 | 14 en...my interests are indeed rather broad...
but those stuffs you list are related closely ah...
stem cells can be neuronal stem cell,
in order to get nerve regeneration,
we need to use tissue engineering methods and 3D scaffolds with nano-
features....
China is good and growing very fast...a lot of money for research...
about
stuff |
|
c*******u
发帖数: 175 | 15 flavor-selectable super strong hydrogel as scaffold for stem cell
differentiation with no toxicity |
|
d****i
发帖数: 360 | 16 Viability and differentiation of neural precursors on hyaluronic acid
hydrogel scaffold
Pan, L., Ren, Y., Cui, F., Xu, Q. 2009 Journal of Neuroscience Research 87
(14), pp. 3207-3220
e-mail: x**********[email protected] |
|
d****i
发帖数: 360 | 17 Biomacromolecules
Polysaccharide Multilayer Nanoencapsulation of Insulin-Producing β-Cells
Grown as Pseudoislets for Potential Cellular Delivery of Insulin; Zheng-
liang Zhi*†, Bo Liu‡, Peter M Jones‡ and John C Pickup;
ASAP.
And
Biomacromolecules
Enhanced Cell Ingrowth and Proliferation through Three-Dimensional
Nanocomposite Scaffolds with Controlled Pore Structures; Kee-Won Lee,
Shanfeng Wang, Michael J. Yaszemski and Lichun Lu; ASAP
Please e-mail to: x**********[email protected] |
|
x*****g
发帖数: 82 | 18 国内有机硕士毕业，博士期间的工作经历如下
Synthesis and characterization of Polymer & monomer, Modification functional
molecules to polymer scaffolds
The application of synthetic biocompatible polymers, like drug and gene
delivery
Operating Instrumentals, NMR, HPLC, GPC, MS (MALDI-TOF),TEM, Confocals IR,
DLS etc.
publication现在只有一篇jacs和国内时候发的几篇有机方向文章，还有4-5篇已经投出
或者即将投出。
明年五月份毕业，不知道能不能在毕业前找到位子，其实也想找工业的，但是自己口语
不好，没信心。
将来何去何从还没有打算。
罗嗦了一大堆，请大家给点建议，现在心里很忐忑，谢谢 |
|
t****t
发帖数: 45 | 19 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
m*******7
发帖数: 28 | 20 【 以下文字转载自 Biology 讨论区 】
发信人: maodun107 (maodun), 信区: Biology
标 题: paper help
发信站: BBS 未名空间站 (Wed Oct 19 20:38:42 2011, 美东)
http://www.liebertonline.com/doi/abs/10.1089/ten.2006.12.1553
Polyelectrolyte nano-scaffolds for the design of layered cellular architectu
res. Tissue Engineering 2006, 12:1553-1563.
doi:10.1089/ten.2006.12.1553.
email: c*****[email protected]
thanks a lot. |
|
h*****b
发帖数: 492 | 21 Title: Design and examination of an antioxidant-containing polyphosphazene
scaffold for tissue engineering
Journal: Polymer Chemistry
Issue: 3
Volume: 3
P778-786
please send the paper to h*****[email protected]
Thanks |
|
L****r
发帖数: 333 | 22 嘎tech化学 got Younan Xia, in 2011 he publsihed 11 Angewandte Chemie
International Edition.
(1) Silver nanocrystals with concave surfaces and their optical and surface-
enhanced Raman scattering properties
Xia, X.; Zeng, J.; McDearmon, B.; Zheng, Y.; Li, Q. and Xia, Y. Angewandte
Chemie International Edition 2011, 50, 12542-12546 (VIP article, highlighted
on the inside cover).
(2) Shape-controlled synthesis of copper nanocrystals in an aqueous solution
with glucose as a reducing agent and hexadecyl... 阅读全帖 |
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b****u
发帖数: 2771 | 23 You must be good at both execution of experiment and people skills.
Don't panic about the job.
HPLC is not rocket science, you just need a bit training and hands-on
experience. I have seen many ppl learned on the job and performed well.
Best way to learn is to search for established method/protocol in your
company. know your analyte and understand every detail in the method. This
will give you a solid foundation and scaffold to build your own mehtod. It
doesn't hurt to consult senior ppl (better... 阅读全帖 |
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w******e
发帖数: 953 | 24 Tissue Engineering - Part A
Volume 20, Issue 1-2, 1 January 2014, Pages 92-102
Odontogenic induction of dental stem cells by extracellular matrix-inspired
three-dimensional scaffold |
|
w******e
发帖数: 953 | 25 nternational Journal of Molecular Sciences
Volume 16, Issue 3, 2 March 2015, Pages 4666-4681
A hyaluronan-based scaffold for the in vitro construction of dental pulp-
like tissue |
|
w******e
发帖数: 953 | 26 标 题:
发信站: BBS 未名空间站 (Mon Apr 20 11:41:37 2015, 美东)
International Journal of Molecular Sciences
Volume 16, Issue 3, 2 March 2015, Pages 4666-4681
A hyaluronan-based scaffold for the in vitro construction of dental pulp-
like tissue |
|
j***j
发帖数: 560 | 27 1. Chemistry of 1,2,5-thiadiazoles. IV.Benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,
5]thiadiazole
Komin, Andrew P. and Carmack, Marvin
Journal of Heterocyclic Chemistry,12(5), 829-33; 1975
2. Functionalization of the benzo[c][1,2,5]thiadiazole scaffold via Mg-, Zn
- and Mn-intermediates
Zimdars, Silvia et al
Synthesis, (8), 1302-1308; 2011
Thanks in advance,
[email protected]
/* */ |
|
w******e
发帖数: 953 | 28 Journal of Endodontics
Volume 34, Issue 4, April 2008, Pages 421–426
Cover image
Basic research—biology
In Vivo Generation of Dental Pulp-like Tissue by Using Dental Pulp Stem
Cells, a Collagen Scaffold, and Dentin Matrix Protein 1 after Subcutaneous
Transplantation in Mice
Rebecca S. Prescott, DDS, MS⁎, Rajaa Alsanea, DDS⁎, Mohamed I.
Fayad, DDS, MS, PhD⁎, Bradford R. Johnson, DDS, MHPE⁎, , ,
Christopher S. Wenckus, DDS⁎, Jianjun Hao, PhD†, Asha S. John,
MS&#... 阅读全帖 |
|
w******e
发帖数: 953 | 29 Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of
MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in
Treatment or Prevention of Osteomyelitis
To cite this article:
Mohiti-Asli Mahsa, PhD, Molina Casey, BS, Diteepeng Thamonwan, BS,
Pourdeyhimi Behnam, PhD, and Loboa Elizabeth G., PhD. Tissue Engineering
Part A. November 2016, 22(21-22): 1258-1263.
doi:10.1089/ten.tea.2016.0063. |
|
w******e
发帖数: 953 | 30 Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of
MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in
Treatment or Prevention of Osteomyelitis
To cite this article:
Mohiti-Asli Mahsa, PhD, Molina Casey, BS, Diteepeng Thamonwan, BS,
Pourdeyhimi Behnam, PhD, and Loboa Elizabeth G., PhD. Tissue Engineering
Part A. November 2016, 22(21-22): 1258-1263.
doi:10.1089/ten.tea.2016.0063. |
|
w******e
发帖数: 953 | 31 Journal of Materials Chemistry B: Materials for biology and medicine
Issue 20, 2015
Paper Nano-silver in situ hybridized collagen scaffolds for regeneration of
infected full-thickness burn skin
J. Mater. Chem. B, 2015,3, 4231-4241
DOI: 10.1039/C5TB00205B |
|
w******e
发帖数: 953 | 32 Journal of Materials Chemistry B
Volume 4, Issue 3, 2016, Pages 505-512
In vitro degradation and angiogenesis of the porous calcium silicate-gelatin
composite scaffold |
|
w******e
发帖数: 953 | 33 Journal of Materials Chemistry B
Volume 1, Issue 26, 14 July 2013, Pages 3304-3313
Controlled release of gentamicin from gelatin/genipin reinforced beta-
tricalcium phosphate scaffold for the treatment of osteomyelitis |
|
w******e
发帖数: 953 | 34 Tissue Eng Part C Methods. 2017 Mar;23(3):169-179. doi: 10.1089/ten.TEC.2016
.0511. Epub 2017 Feb 22.
Preparation and Characterization of a Chitosan/Gelatin/Extracellular Matrix
Scaffold and Its Application in Tissue Engineering.
Wang X1,2, Yu T1,2, Chen G1,2, Zou J3, Li J2,4, Yan J1. |
|
m******y
发帖数: 526 | 35 补课说不上，我写写脑子里还记得的，你再补充阿。。。：）
Piaget 和Vygotsky最大的区别是P认为人的学习是根据个人构建知识的不同而不同，外界
无法起任何作用，最多就是给孩子提供书，但是要他们自己去看（self-directed/initia
ted)；而且在不同的年龄阶段孩子的思维能力是不同的(stage dependent)；V认为人的学
习不能脱离社会环境，只是脱离了社会环境就没有任何意义(scaffolding)，在老师的帮
助下孩子可以达到更高的水平(instruction dependent,ZPD)。（汉语太滥了，翻译的一
窍不通。。。）
也就是：
Cognitive development is a function of:
Piaget: individual construction
Vygotsky:social interaction
此外，另外的区别是：
Role of language:
Piaget: cognition is critical (egocentric speech disappears)
Vygotsky: |
|
t****t
发帖数: 45 | 36 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
l****x
发帖数: 18 | 37 A PhD student position is available in the area of biomaterials and tissue
engineering under the supervision of Dr. Yongxing Liu at the Missouri
University of Science and Technology. Research emphasis is tissue-
engineering approaches for repair and regeneration of musculoskeletal
tissues; current work focuses on the innovation of biomaterials for drug
delivery and cell/tissue scaffolding, and human induced pluripotent stem
cell-based therapy.
Applications with a MASTER degree in bioceramics, bi... 阅读全帖 |
|
b**s
发帖数: 589 | 38 1。这句话好像有点问题
2。如果孔小的话，有可能porosity会减小，也不是好事
3。还要考虑机械强度的问题
其实scaffold应该归到cellular materials的范畴，有很多东西可以借鉴cellular
materials的已经发展很好的理论
relationship between surface area and blood vessel density应该很早就有人作了吧
。他的新颖之处是不是在于老鼠in vivo， 我见到的研究angiogenesis 大多都是在鸡蛋
上做
的
都有SEM
density
area
with |
|
x*o
发帖数: 1037 | 39 来自主题: Macromolecules版 - 问题 Scaffold是什么意思啊?
老看见 |
|
|
w********h
发帖数: 12367 | 41 hehe...又一个science fan...
要知道很多fancy，interesting的的东东都是有其应用前景的啊。
比如self-assembly,photoluminescence,scaffold....
不要舍本逐末，买椟还珠忘记了本来的目的而求复杂美丽的无意义的结论就好了...
，难
PhD
有非
科学
我们最大的荫福不是美，而是吃饭和穿衣）；其次告诉我们人定可胜天（自从VB12合成
，每 |
|
t*****y
发帖数: 30 | 42 Great! Very glad finally there comes a tissue engineering expert!
Any big name or company in west coast? Stanford, Berkeley, Companies?
Right now, the commercial focus is at artificial skins. How about the area of
tissue implants? I remember some time ago, there was a small artificial liver
grown out of a microfabricated scaffold. Knew about this work?
I checked that company in China. Don't know if they own their
intellectual properties or just a sales company. So, if you want to start
one, what |
|
t*****y
发帖数: 30 | 43 Car's speed or fabrication speed? :-)
It is quit slow because it is based on 3D scanning method instead of 2D
planery fab techniques. But I believe with the advances of technology,
multiple scan head would be possible so the throughput can be improved.
Anyway, it is still too early to talk about production.
3D scaffolds would be very useful in tissue engineering.
Still in China now? :-) |
|
b**s
发帖数: 589 | 44 Brave New World Of Biorubber
Volume 4, Number 4 - June 21, 2002
A new material called biorubber could have unlimited possibilities for
engineered tissue, including for making artificial lungs, heart valves and
other elastic tissues, say its developers at the Massachusetts Institute of
Technology.
Safe biodegradable polymers are already employed in drug delivery, tissue
engineering and other applications. Whether seeped in medicine or serving as a
cell-growing scaffold, such polymers are |
|
b**s
发帖数: 589 | 45 来自主题: Macromolecules版 - 泄密 具体的情况不太清楚，我觉得细胞有保护的措施的，例如包裹在
hydrogel 里面
活体指living 生物，如肾脏损伤，就在腹部切开一个口子，直接
在患处生成scaffold和cell 的三维混合体
感应器感应人体的活动的原理就如坦克在行进中瞄准目标，所以
不用怕手术中其他器官跳动的影响
但具他们说不同layer生成不同tissue结构还有困难 |
|
m***n
发帖数: 266 | 46
artificial vascular graft
的研究由来已久。看这段中文我猜大致意思是biodegradable
polymer作scaffold, 即3毫米ID的channel, 用“多微孔的膜”创造一个ECM，
这个"多微孔的膜“的膜估计也是porous polymer, hydrogel之类的，然后把EC注入，
不懂”肝脏或者肾脏的细胞注入“的具体理由，大概就是多提供点cell吧。
我想他最后还要把硅晶片抽出来。
光看中文科技消息，看不出来这个除了用个硅晶片外有什么闪光点。 |
|
b**s
发帖数: 589 | 47 当然，不然怎么“绝对可以作出一篇science的文章”。嘿嘿。我们用的是latex体系
，可以把growth
factor包在latex颗粒里，然后把降解酶也包在latex颗粒里,初期scaffold降解很慢，当
降解酶被缓慢释放出来后，降解速度就会显著增加。这样你可以把growth factor, 降解
酶，和ink放在不同的syringe里，就可以实现局部和时间上的调控.
you
should
通过控制3种材料混合的比例，可以实现无限的组合。比如3种材料含有不同的growth
成
以
一 |
|
m***n
发帖数: 266 | 48 最近看了一系列的相关文章，想和大家聊聊。
大体上大家伙都差不多，找种细胞，
找个scaffold,体外养会儿，植入体内看看，做一堆表征。
fancy点的无非就是加点growth factor(controlled release就更酷)，
或者折腾细胞(机械锻炼以增强强度和细胞alignment等等)或者某种
3D culture设备(不是指boss的仪器，boss不要误会：)
总体感觉是现在的TE真是容易发文章，而且感觉总体内在科技含量都不高，
大牛也一样，not very scientifically sound，Harvard MedScool的
一帮MD的文章尤其惨不忍睹，那个stain做的差呀。
可是还是觉得TE是未来一个非常重要的发展方向。搞BIO最终不就是
为了治病么，所以个人觉得将来在这上面NIH和NSF会投更多钱，
不象drug delivery有大药厂的研发撑着。纯分子生物学,基因调控和
蛋白质表达最后都可以在TE上找到落脚点。
再就是Engineer在其中起的作用。常有人argue说现在什么BioEngineering,
不就是分子生物学和医学吗？其实是低估了en |
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b**s
发帖数: 589 | 49 我都懒得反驳你，看了几篇文章就来对TE妄加评论。哈哈，不要生气。我就问一个简
单的问题：用PLGA为材料，要你做一个scaffold，必须满足时间(growth factor
controlled release)，空间(内部和外部)，组成,机械强度可以控制。还不能加热，不能
用有机溶剂。你现在能做的到吗？我现在可以说我们基本已经完成了这些指标。但我们从
不发文章,我认为发文章是很无聊的事情。你以为Harvard MedScool的文章惨不忍睹。人
家没有把好东西拿出来。首先你从文章来看一个行业的发展，就完全错误。发文
章都是要有经费(当然，混博士的除外)，而每个经费都是要有一个项目，而每个项目都是
有一个大背景的。我来给你分析一下。Tissue engineering的终极目的就是替换器官。那
好，人体都有什么器官？首先是皮肤。人工皮肤也是目前为止唯一成功的Tissue
engineering产品.再就是心脏，当时有个project叫“LIFE”，聚集了一大帮人，最后失
败了。MIT的人工肝脏，最后也失败了。
传递，压力3D控制是可以符合基本机械和化工原理的(虽然复杂得多， |
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b**s
发帖数: 589 | 50 1. Aqueous formula, can incorporate soluble protein
2. Can deposit in open air.
3. Solidify instantly under room temperature, can support upper layer
4. No organic solvent used, no heating, no UV light
5. 100 micron feature size
6. Can make heterogeneous scaffold structure (forget about MIT's 3D printer)
7. Temporal, spatial, compositioanl and mechanical controllable in 3D pattern.
I have developed a ink satisfies all the requirments, that's the reason my
company hire me, top secret, haha.
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