p**p
发帖数: 2493 | 1 在网上看到的，觉得对第一次买房的会有帮助。
Buying a home in Texas is similar to purchasing a home in some of the other
states and very different from yet other states.
Texas is one of nine community property states. Generally speaking, a
community property state is a state in which property accumulated during a
marriage (other than by gift or inheritance or property owned by one spouse
prior to the marriage), is equally owned by each spouse.
Texas homeowners may apply for a Homestead Exemption on their principal
re... 阅读全帖 |
|
e*i
发帖数: 10288 | 2 General answer : NO.
Unless your lot is 1 acre or more.
I did some research for my daughter last year. :P
ARTICLE 2.01 GENERAL PROVISIONS*
Sec. 2.01.001 Dangerous wild animals
Dangerous wild animals, as defined in V.T.C.A., Health and Safety Code,
section 822.101, shall be regulated in accordance with the provisions of V.T
.C.A, Health and Safety Code, chapter 822, subchapter E, section 822.101 et
seq. (Ordinance adopting Code)
ARTICLE 2.02 LIVESTOCK AND FOWL*
Division 1. Generally
S... 阅读全帖 |
|
e*i
发帖数: 10288 | 3 General answer : NO.
Unless your lot is 1 acre or more.
I did some research for my daughter last year. :P
ARTICLE 2.01 GENERAL PROVISIONS*
Sec. 2.01.001 Dangerous wild animals
Dangerous wild animals, as defined in V.T.C.A., Health and Safety Code,
section 822.101, shall be regulated in accordance with the provisions of V.T
.C.A, Health and Safety Code, chapter 822, subchapter E, section 822.101 et
seq. (Ordinance adopting Code)
ARTICLE 2.02 LIVESTOCK AND FOWL*
Division 1. Generally
S... 阅读全帖 |
|
S*******h
发帖数: 7021 | 4 【 以下文字转载自 USANews 讨论区 】
发信人: fanyiding (凡一丁), 信区: USANews
标 题: 德克萨斯上书九庭，支持川总旅行禁令
发信站: BBS 未名空间站 (Wed Feb 15 20:20:43 2017, 美东)
大德州威武
Texas attorney general backs Trump in travel ban litigation
President Donald Trump acted well within his authority in issuing his
executive order on immigration, Texas Attorney General Ken Paxton told a
federal appeals court on Wednesday.
Paxton, a Republican, attached a brief in asking the 9th U.S. Circuit Court
of Appeals in California for permission to argue in... 阅读全帖 |
|
c*******o
发帖数: 5387 | 5 【 以下文字转载自 Parenting 讨论区 】
发信人: LoanSeeker (Need Better Loan), 信区: Parenting
标 题: 爬藤的家长注意喽
发信站: BBS 未名空间站 (Mon Jan 21 16:04:05 2013, 美东)
下面的文章要读一读。
文章摘要如下：鸡头>凤尾原则是被广泛观察到的现象所支持的。同一入学新生，相比
于去与自己水平相当的大学，选择去比自己水平高很多的藤校是很不明智的。所以AA造
成对黑青年“毁人>诲人”。
如斯成立，则亚裔家长推自个的娃也须小心。如家长推了10年娃也没学会自推，那靠补
习班、凑活动经验上了藤校也是去给牛娃当分母做尾巴去了，也许不如去一州校帮助大
。学费省下来还可以投资给娃当个天使基金啥的。
发信人: yariguy (yari guy), 信区: Parenting
标 题: Re: Support Asian American students!!!
发信站: BBS 未名空间站 (Mon Jan 21 10:52:51 2013, 美东)
The Say Irony of Affirmativ... 阅读全帖 |
|
T***c
发帖数: 17256 | 6 其实这边老中特别是有小孩的老中们这么激动的原因，并不是因为都指望自己的小孩去
打NBA--那个是比高考残酷百倍的真。千军万马过独木桥，能有实力竞争大学奖学金的
都是极少数。而是希望自己的小孩学习之余，以后起码可以大声的说“我喜欢打球”不
给周围老中视为异类（“你看我家孩子都谈钢琴”），在球场上分拨pickup时少遇到几
次“一黑二白三花四黄”的场景。
Hoop Dreams
How Jeremy Lin is affecting the lives of thousands of Asian children.
by Keane Shum
There is one, and only one, very specific demographic rooting against Jeremy
Lin, the sudden, inexplicable new star point guard for the New York Knicks.
They are the conspirators behind an age-old scheme, one in which the dream... 阅读全帖 |
|
l*****9
发帖数: 9501 | 7 Knicks held back news of Jeremy Lin's knee injury to sell playoff tickets?
By Royce Young | NBA Blogger
Hard to have playoff Linsanity if Jeremy Lin's injured and not playing,
right? At least that's how the Knicks evidently saw it.
Lin had knee surgery to repair a torn meniscus and he'll be out at least six
weeks, which leads right up to the playoffs. Whether or not he'd be ready
then is up in the air. But the Knicks didn't want you to know that, instead
keeping Lin's injury quiet so they coul... 阅读全帖 |
|
e********3
发帖数: 18578 | 8 http://www.thedreamshake.com/2014/1/3/5249000/houston-rockets-j
Did you know Jeremy Lin leads the league in many offensive metrics while
being near last in others? We try to lift the veil clouding the opinions of
the Rockets' point guard as we go searching for Jeremy Lin.
There are two things that I really dislike, one is writing introductions for
anything and the other is irrational reactions to NBA players. Obviously
this isn't going to be a whole post about why I don't like writing
introducti... 阅读全帖 |
|
z*****a
发帖数: 3809 | 9 蛇人写的 MMQB 文章
http://mmqb.si.com/2013/09/18/richard-sherman-san-francisco-49e
We Compete Against Ourselves
September 18, 2013 by Richard Sherman
Sherman’s fourth-quarter interception led to a field goal that put the
Seahawks up 22–3 on the way to a definitive 29–3 win over the division-
rival Niners. (Robert Beck/Sports Illustrated) -
I’m no golfer.
But I do own a set of clubs. I’m taking lessons, and I’ve hacked away on
five or six occasions with my teammate Brandon Browner. On Tuesday, after we... 阅读全帖 |
|
b*****n
发帖数: 2333 | 10 哈哈 好蠢的裁判 上次裁colts对德佬那场也是 各种乱判
CINCINNATI -- Here's the transcript on referee Jeff Triplette's explanation
on why he reversed the play where the Colts appeared to stop the Bengals on
fourth-and-goal from the 1-yard line late in the first half of their 42-28
loss:
Were there differing views among the officials about whether BenJarvus Green
-Ellis was down initially?
Triplette: There was discussion about whether the runner was touched down at
the goal line or not.
What did you see in the review?... 阅读全帖 |
|
m****t
发帖数: 25 | 11 Gosh, guys! Please refer all the sentences Kubiak said:
Kubiak doesn’t have much to say about Peyton’s future
Posted by Michael David Smith on January 20, 2015, 2:23 PM EST
Gary Kubiak
AP
New Broncos head coach Gary Kubiak doesn’t have much to say about whether
he expects Peyton Manning to be his quarterback.
Kubiak said at his introductory press conference today that he has talked to
Manning, but he doesn’t know whether Manning will retire. Kubiak also didn
’t say whether the Broncos want to pa... 阅读全帖 |
|
g*2
发帖数: 658 | 12 Overview of running injuries of the lower extremity
Author
Lisa R Callahan, MD Section Editor
Karl B Fields, MD Deputy Editor
Jonathan Grayzel, MD, FAAEM
All topics are updated as new evidence becomes available and our peer review
process is complete.
Literature review current through: Feb 2012. | This topic last updated: Oct
20, 2011.
INTRODUCTION — Running is one of the world’s most popular forms of
exercise, with millions of regular participants. In the United States alone,
up to 40 mil... 阅读全帖 |
|
J********i
发帖数: 50662 | 13 http://www.businessweek.com/news/2011-03-17/japan-nuclear-disas
黑幕很多，事情大条了
By Jason Clenfield
March 18 (Bloomberg) -- The unfolding disaster at the Fukushima nuclear
plant follows decades of falsified safety reports, fatal accidents and
underestimated earthquake risk in Japan’s atomic power industry.
The destruction caused by last week’s 9.0 earthquake and tsunami comes less
than four years after a 6.8 quake shut the world’s biggest atomic plant,
also run by Tokyo Electric Power Co. In 2002 and ... 阅读全帖 |
|
z*********n
发帖数: 94654 | 14 哈哈，这个周末电视台，电台老听到大家在讲这个最新的study,
结论是：科学无法验证有机蔬菜比普通蔬菜对人更好
最多可能农药残余更小，但是这个差别也无证据显示有啥重要影响
最后一个法宝，就是全人类的角度了，organic gardening，对环境影响比较小，run
off农药化肥比较少，对周围的大江大河有好处...
所以，农友们，改用化肥就用吧，哈哈哈，特别责任感强的，关注全人类的，少用点吧
:D
http://www.npr.org/blogs/thesalt/2012/09/04/160395259/why-organ
Yes, organics is a $29 billion industry and still growing. Something is
pulling us toward those organic veggies that are grown without synthetic
pesticides or fertilizers.
But if you're thinking that organic produce will help you stay... 阅读全帖 |
|
c***x
发帖数: 1826 | 15 Richard Dawkins’ Law Delusion
AIX-EN-PROVENCE – Richard Dawkins is one of the great minds of our time;
yet in his just-published memoir, Brief Candle in the Dark: My Life in
Science, he notes that great minds often err when they leave their field of
expertise. He cites the great astronomer Fred Hoyle, whose book The Nature
of the Universe was essential reading a half-century ago. When Hoyle turned
to biology, he went astray. The same thing happens to Dawkins when he turns
from science to law.
Da... 阅读全帖 |
|
B*********e
发帖数: 86 | 16 ([email protected])
回帖_BiggeyIssue_to wonderingmm jiuselu Asdfeqo et al_mitbbs_2018-03-08 ~ 03
[email protected]_1633-.docx
([email protected])
回帖_BiggeyIssue_to wonderingmm jiuselu Asdfeqo et al_mitbbs_2018-03-08 ~ 03
[email protected]_2224-.docx
([email protected])
回帖_BiggeyIssue_to wonderingmm jiuselu Asdfeqo et al_mitbbs_2018-03-08 ~ 03
[email protected][email protected]_2330-_.docx
([email protected])
回帖_BiggeyIssue_to wonderingmm jiuselu Asdfeqo et a... 阅读全帖 |
|
G****e
发帖数: 11198 | 17 http://www.nytimes.com/2011/01/09/education/edlife/09ap-t.html?
January 8, 2011
Is Law School a Losing Game?
By DAVID SEGAL
IF there is ever a class in how to remain calm while trapped beneath $250,
000 in loans, Michael Wallerstein ought to teach it.
Here he is, sitting one afternoon at a restaurant on the Upper East Side of
Manhattan, a tall, sandy-haired, 27-year-old radiating a kind of surfer-dude
serenity. His secret, if that’s the right word, is to pretty much ignore
all the calls and lett... 阅读全帖 |
|
l******a
发帖数: 16364 | 18 我以前查过，转基因就不能称为有机。
GMO Food: Genetically-modified organisms. This refers to the act of
scientifically modifying the genetic structure of an organism. The resultant
organism will have specifically defined characteristics. This is frequently
done with seeds for crops. In the U.S., most of our soybeans, corn, cotton,
and canola are genetically modified. Recently, the U.S. allowed GMO alfalfa
to be planted without restriction. The genetically modified seeds can be
patented. After all, the research to... 阅读全帖 |
|
n****m
发帖数: 1283 | 19 I think the key point that whether the charity fund is legal for charity
matching or tax return should be mentioned as well. That is what ACCEF
advertised.
I modified slightly.
Dear Sir/Madam,
I am writing to inquire about whether an event happened in March, 2013 could
be defined as charity fraud according to the law.
On March 8th, March 9th and March 14th, an ID called lilykang (real name:
Lei Kang) began to post on the biggest Chinese community discussion forum in
United States (www.mitbbs.com... 阅读全帖 |
|
d********f
发帖数: 43471 | 20 【 以下文字转载自 USANews 讨论区 】
发信人: datou (datou), 信区: USANews
标 题: 大家都有投票权了！（不管是不是公民，合法的非法移民都可以投票！）
发信站: BBS 未名空间站 (Mon Apr 4 12:54:08 2016, 美东)
最高法院今天投票表决了不管是不是公民，不管你是合法非法，不管你年龄，都可以投
票。如果你想保护自己的利益，请积极参与投票。全文如下：
http://www.nytimes.com/2016/04/05/us/politics/supreme-court-one
WASHINGTON — The Supreme Court on Monday unanimously ruled that states may
count all residents, whether or not they are eligible to vote, in drawing
election districts. The decision was a major statement on the meaning of a
fund... 阅读全帖 |
|
e******u
发帖数: 3206 | 21 【 以下文字转载自 USANews 讨论区 】
发信人: daemonself (mit行为艺术专业博士后导师), 信区: USANews
标 题: 让左x high一晚上，现在开始进入打脸时刻
发信站: BBS 未名空间站 (Thu Jan 26 08:44:54 2017, 美东)
https://www.uscis.gov/news/fact-sheets/public-charge-fact-sheet
Public Charge Fact Sheet
Released April 29, 2011
Introduction
Public charge has been part of U.S. immigration law for more than 100 years
as a ground of inadmissibility and deportation. An individual who is likely
at any time to become a public charge is inadmissible to the United States
an... 阅读全帖 |
|
发帖数: 1 | 22 Dear Daughter:
亲爱的女儿：
As we drove off from Columbia, I wanted to write a letter to you to tell
you all that is on my mind.
当我们开车驶出哥伦比亚大学的时候，我想写一封信给你，告诉你盘旋在我脑中的想法
。
First, I want to tell you how proud we are. Getting into Columbia is a
real testament of what a great well-rounded student you are. Your academic,
artistic, and social skills have truly blossomed in the last few years.
Whether it is getting the highest grade in Calculus, completing your elegant
fashion design, successfully selling you... 阅读全帖 |
|
l*****o
发帖数: 26631 | 23 Long-Term Health Risks and Benefits Associated with Spay / Neuter in Dogs
Laura J. Sanborn, M.S.
May 14, 2007
Precis
At some point, most of us with an interest in dogs will have to consider
whether or not to spay / neuter our pet. Tradition holds that the benefits
of doing so at an early age outweigh the risks. Often, tradition holds sway
in the decision-making process even after countervailing evidence has
accumulated.
Ms Sanborn has reviewed the veterinary medical literature in an exhaustive
a... 阅读全帖 |
|
t******g
发帖数: 3641 | 24 Your Dog Is Watching You
By TARA PARKER-POPE
He knows you've got your eye on him.He knows you’ve got your eye on him.
You may be teaching your dog new tricks, without even trying.
Dogs are constantly learning from the reaction of human owners, picking up
facial cues and anticipating their owner’s behavior, new research suggests.
The findings, published online in the journal Learning and Behavior, show
that dogs essentially are always in training, and help explain how many
owners unknowingly teac... 阅读全帖 |
|
t******g
发帖数: 3641 | 25 FIV有两种检测方式
我们做的事ELISA的，据说，neg就是neg，但是如果是pos可能需要复查另外那种方式的
网上找了一下：
http://www.neighborhoodcats.org/HOW_TO_RELEASING_FIV_FELV_POSIT
这篇文章非常好，大家最好看一下。
一个是六个月以下的不准
一个是pos FIV， FeLV可能不准，可能是打过疫苗有抗体，也有可能抗原还未进入白细
胞，回头，猫自己好了的
另外文章说，feral cat，一个collony很多得病的，根本原因是营养不良，环境不好。
移走病猫不能解决问题。改善的方式是让他们得到更好的照顾。文章说，健康的成年猫
，其实不容易得FeLV FIV的。
另外kc有益于减少得病率，因为传染主要是两个途径，深度咬伤，母猫哺乳。如果男猫
neuter了，不易打架，就减少咬伤概率。母猫spay了，不会生小猫，减少了哺乳造成小
病猫的概率。
Initial test results are not always reliable, but with ferals, life or death
decisions are... 阅读全帖 |
|
t******g
发帖数: 3641 | 26 http://www.neighborhoodcats.org/HOW_TO_RELEASING_FIV_FELV_POSIT
我喜欢最后那段的一句话：“it's about improving the situation, not about
making it perfect. ”
希望和在帮助小动物的路上还在犹豫的宠友们一起共勉。我不会再犹豫是不是给猫找到
了perfect的家，只要能达到threshold的，就比他们在外面流浪强。这就是improving
。但是如果我因为不忍心，不去做，那么他们仍然还是在流浪，有可能明天后天就不在
了……
Releasing FIV/FeLV Positive Cats
Neighborhood Cats opposes euthanizing any feral cat simply because he or she
tests positive for FIV (feline immuno-deficiency virus) or FeLV (feline
leukemia virus). If the cat shows no act... 阅读全帖 |
|
j*****7
发帖数: 10575 | 27 http://www.ccel.org/ccel/boettner/predest.iii.iii.html
Chapter IV
The Sovereignty of God
Every thinking person readily sees that some sovereignty rules his life. He
was not asked whether or not he would have existence; nor when, where, or
what he would be born; whether in the twentieth century or before the flood;
whether white or Negro; whether in America or in China. It has been
recognized by Christians in all ages that God is the Creator and Ruler of
the universe, and that as the Creator and ... 阅读全帖 |
|
G*M
发帖数: 6042 | 28 Bible Course - Christian Doctrine
Lesson 8
God the Holy Ghost
Lord's Day 20 - 22
God the Holy Ghost
In Lord's Day 11-19, on the basis of articles 2 - 7 of the Apostles' Creed,
there is spoken of Jesus Christ and His work. In Lord's Day 20-22, on the
basis of the last five articles of the Creed, there is spoken of the Holy
Ghost and His work, who He is and what He does in order that we may be
partakers in the deliverance which Christ has merited for us.
Lord's Day 20
Q. 53. What dost thou believe... 阅读全帖 |
|
D*****r
发帖数: 6791 | 29 http://www.franknelte.net/Nelte_HTML/LUCIFER.htm
In the English version of the Bible the name "Lucifer" appears only one time
, in Isaiah 14:12. This verse reads:
"How are you fallen from heaven, O LUCIFER, son of the morning..." (Isaiah
14:12)
Now the word "Lucifer" is not an English word, but a LATIN word. And so the
question is:
WHO GAVE THE WORLD THIS LATIN NAME "LUCIFER"? AND WHY DID THEY GIVE US THIS
LATIN NAME?
HOW WE CAME TO HAVE THIS NAME "LUCIFER"
In 382 A.D. Pope Damasus commissioned ... 阅读全帖 |
|
l**********t
发帖数: 5754 | 30 Chapter 1.6 The Blood And The Believer’s Access
The Blood has satisfied God; it must satisfy us also. It has therefore a
second value that is manward in the cleansing of our conscience. When we
come to the Epistle to the Hebrews we find that the Blood does this. We are
to have “hearts sprinkled from an evil conscience” (Hebrews 10:22).
This is most important. Look carefully at what it says. The writer does not
tell us that the Blood of the Lord Jesus cleanses our hearts, and then stop
there in h... 阅读全帖 |
|
l**********t
发帖数: 5754 | 31 Chapter 1.6 The Blood And The Believer’s Access
The Blood has satisfied God; it must satisfy us also. It has therefore a
second value that is manward in the cleansing of our conscience. When we
come to the Epistle to the Hebrews we find that the Blood does this. We are
to have “hearts sprinkled from an evil conscience” (Hebrews 10:22).
This is most important. Look carefully at what it says. The writer does not
tell us that the Blood of the Lord Jesus cleanses our hearts, and then stop
there in h... 阅读全帖 |
|
J*****3
发帖数: 4298 | 32 笑死我了，还来玩黑白颠倒的吗？
我给你全文吧，这么撒谎的话，是耶稣教的吗？撒谎神不喜悦
这篇文章是基督教手册用来歪曲人类进化不成立的，是JAMES给我了文章的一个LINK，但是文章看不全，我根据作者名字找到了全文，也读了，发现，文章根本没有说进化不成立，他们断章取义，黑白颠倒地撒谎。我BS这种恶心的行为。
“5、大概在97年，一个著名的非基督徒考古学家，在非洲发现的所谓人类化石，把一
直以来公认的人类进化顺序，猿人——直立人——能人——智人，给彻底推翻了。目前
人类进化链条重新归零，变为空白。”
原文
http://news.nationalgeographic.com/news/2010/05/100506-science-
翻译：
“Even if the new result doesn't quite settle the debate about whether
Neandertals mixed with modern humans, it does underscore how different they
were from our own lineage. And t... 阅读全帖 |
|
G*******s
发帖数: 4956 | 33 首先给大家看个故事，然后再探讨经文：
林慈信牧师的《基督教教义发展史》中提到这个：
http://blog.sina.com.cn/s/blog_622134fd01013i7l.html
基督论的发展史(1)：基督论的争辩
HISTORY OF THE DOCTRINE OF CHRIST (1):
The Christological Controversies
(Louis Berkhof, A History of Christian Doctrines, pp. 101ff.)
基督论与三位一体之问题的关联
Connection of Christological and Trinitarian Problems
基督论的难题可以从一般神学（译注：神论）方面，与拯救论方面来加以
研究。早期教父对基督论拯救方面的关系，虽未曾加以轻忽，但他们在重要的讨论上却
没有重视。在三位一体争论的气氛中，他们从一般神学（译注：神论）方面来研究基督
，乃天经地义的事，而三位一体争论所导致的决定则是，基督为神的儿子，事与父同质
的，因此祂是神。从此而发生的问题，就是基督... 阅读全帖 |
|
l*****a
发帖数: 38403 | 34 Thomas Jefferson, (1743-1826) 3rd American president, author, scientist,
architect, educator, and diplomat. Deist, avid separationist.
"Millions of innocent men, women, and children, since the introduction of
Christianity, have been burned, tortured, fined, and imprisoned, yet we have
not advanced one inch toward uniformity. What has been the effect of
coercion? To make one half of the world fools and the other half hypocrites.
" [Notes on Virginia]
"I do not find in orthodox Christianity one re... 阅读全帖 |
|
n********n
发帖数: 8336 | 35 Free Will and Determinism
http://www.trinity.edu/cbrown/intro/free_will.html
This is a quick look at issues concerning the nature of free agency. There
are three important questions about free will and determinism, but only one
of them is really a philosophical question. Ayer mentions all three
questions in his opening paragraph (Bailey, p. 558): "It is commonly assumed
that men are capable of acting freely, in the sense that is required to
make them morally responsible, and that human behavior ... 阅读全帖 |
|
J*******g
发帖数: 8775 | 36 记得本版有几名反基是Dawkins的崇拜者。
发信人: cjdlx (五柳), 信区: LeisureTime
标 题: Re: 请飞天评论：from science to law
发信站: BBS 未名空间站 (Sat Jan 9 20:55:40 2016, 美东)
Richard Dawkins’ Law Delusion
AIX-EN-PROVENCE – Richard Dawkins is one of the great minds of our time;
yet in his just-published memoir, Brief Candle in the Dark: My Life in
Science, he notes that great minds often err when they leave their field of
expertise. He cites the great astronomer Fred Hoyle, whose book The Nature
of the Universe was essential reading... 阅读全帖 |
|
a*****g
发帖数: 19398 | 37 #!/usr/bin/env pike
// legal.pike - Count the number of legal go boards.
// Copyright 2005 by Gunnar Farneb?ck
// [email protected]
/* */
//
// You are free to do whatever you want with this code.
//
//
// This program computes the number of legal go board configurations
// for a rectangular board of a given size. It is efficient enough to
// handle boards up to 8x8 within minutes and up to 11x11 in less than
// 24 hours (on a fast computer). For rectangular boa... 阅读全帖 |
|
z***t
发帖数: 10817 | 38 【 以下文字转载自 Stock 讨论区 】
发信人: zhangfei (Leo Kamis), 信区: Stock
标 题: Re: 谁给讲讲NVDA和INTC的关系
发信站: BBS 未名空间站 (Fri Nov 11 12:53:28 2016, 美东)
1. Intel still has better Silicon process. NVidia depends on TSMC on
manufacturing.
2. Intel missed and behind Nvidia AI several years. Right now is doing catch
up.
* buy Nervana @about 400m.
* buy Movidius.
* change Xeon Phi roadmap to include deep learning specific instruction.
* buy Altera @16.7B, trying co-processing concept.
* All the other AI investment throug... 阅读全帖 |
|
发帖数: 1 | 39 Which language has the brightest future in replacement of C between D, Go
and Rust? And Why?
In spite of my status and obvious bias as co-creator of D, I'll do my best
to answer candidly; I follow Go and Rust, and I also definitely know where D
's dirty laundry is. I'd encourage people with similar positions in the Rust
and Go communities to share their honest opinion as well. So here goes.
First off, C++ needs to be somewhere in the question. Whether it's to be
replaced alongside C, or be one o... 阅读全帖 |
|
b********r
发帖数: 9 | 40
how
1.extract the total mRNA
2.use restriction enzyme to cut out the desired gene
3.design a primer and do a PCR
4.Sequence the DNA and compare with those published sequences to see whether
it encodes the kind of enzyme you want.
5.Southern Blot to see whether pure and whether it is there.
6.Express the cDNA in Ecoli and check protein activity.
7.translate the sequenced cDNA to see whether you got the correct protein.
8.Sequence the expressed protein and back translate part of the AA's that has |
|
G*P
发帖数: 214 | 41 不过第一段英语写得真差，之前如果完全不知道此事的话，这段话可以让你云里雾里，
而且混淆当事人
http://www.nature.com/news/2011/110608/full/474140a.html
Whistle-blower claims his accusations cost him his job
University denies it retaliated against researcher who questioned supervisor
's data.
Eugenie Samuel Reich
Zebrafish embryos are at the centre of a dispute over research integrity.
Zebrafish embryos are at the centre of a dispute over research integrity.R.
KESSEL/VISUALS UNLIMITED/CORBIS
After months of friction that culminated in his open... 阅读全帖 |
|
z*t
发帖数: 863 | 42 Beutler得奖，Medzhitov会不会有意见？
http://www.sciencedirect.com/science/article/pii/S1074761309002
Ruslan Medzhitov
This year marks the 20th anniversary of a publication (Janeway, 1989) that
in many ways revolutionized our understanding of the immune system. As part
of the proceedings of the Cold Spring Harbor Symposium on Immune Recognition
, the paper authored by the late Charles A. Janeway, Jr. was not a standard
peer-reviewed publication. In fact, Charlie used to refer to it as “the
best paper I've ... 阅读全帖 |
|
j****1
发帖数: 81 | 43 Thank you very much for the comments. I revised abstract. It's attached
below.
----------------------------------------------------------------------------
----------------------------
Colon cancer is the second highest cause of cancer deaths in the United
States (Markowitz & Bertagnolli, 2009). It is well known that APC (
adenomatous polyposis coli) loss predisposes patients to colon cancer, which
has been primarily attributed to the over activation of Wnt signaling.
Although controversial, can... 阅读全帖 |
|
t*******y
发帖数: 21396 | 44 http://www.theguardian.com/science/2014/apr/01/stem-cell-scient
Stem cell scientist Haruko Obokata found guilty of misconduct
Committee in Japan says it found evidence of falsification and fabrication
– offences that constitute research misconduct
A young researcher who shot to fame in scientific circles when she published
an apparently radical and simple way to create stem cells has been found
guilty of misconduct by a committee charged with investigating her work.
Haruko Obokata, at the Riken ... 阅读全帖 |
|
s******9
发帖数: 283 | 45 http://www.sciencemag.org/content/344/6179/24.full
Chasing the Money
Jennifer Couzin-Frankel
As constraints take hold in biomedicine, scientists are forced to adapt.
Money. It is what fuels research, and these days, it's almost all biomedical
scientists in the United States can talk about.
They've been buffeted by funding swings at the National Institutes of Health
(NIH), their field's primary benefactor. And now they're anxious about the
future, as Congress tries to rein in debt by slowing gove... 阅读全帖 |
|
v**********m
发帖数: 5516 | 46 http://m.f1000research.com/articles/3-291/v1
波士顿地区的千老联合会的报告，文章提到的问题非常典型和深刻，该文已经在圈内得
到了包括大佬们的极大肯定(恐惧)。
OPINION ARTICLE
Shaping the Future of Research: a perspective from junior scientists[v1;
ref status: approved 1, approved with reservations 1,http://f1000r.es/4ug]
Gary S. McDowell1*, Kearney T. W. Gunsalus2*, Drew C. MacKellar3, Sarah A.
Mazzilli4, Vaibhav P. Pai1, Patricia R. Goodwin5, Erica M. Walsh6, Avi
Robinson-Mosher7, Thomas A. Bowman8, James Kraemer9, Marcella L. Erb10, Eldi
Schoenfeld1... 阅读全帖 |
|
