j*****5
发帖数: 24 | 1 i am trying to making a SHRNA lentivirus vector ( pRNAT from Gene script),
however, i was unable to get positive clone, And the yield of the plasmid
is also very low ( around 200ng/ul) I used HB101 E.coli competent cells,
Anybody can help me out? Thanks in advance. |
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g*********3
发帖数: 177 | 2 谢谢toll的回答。不好意思，我有一个typo，已经改正过来了。Ct difference in
sample is 6.
嗯，Input确实可以用1%来做，不过在QPCR之前，我测了浓度，也做了QPCR dilution
test，所以这个应该不是问题。
你提的建议很好，我其实也做了negative primer qpcr,做出来的Ct是NA，说明CHIP结
果的特异性还是不错的。
我说的control就是你提到的KO cell line.
"最后用chip/input%的方式来表达，不要任何的normalization"
这其实也是我最感兴趣的信息，我查了几篇paper，发现别人用HA tag做的CHIP，yield
也很低，大概只能pull down 2%左右的DNA （方法也是通过比较Input和Sample这个某
个特定region的QPCR ct）。
我知道IP nuclear protein效率大概是10-20%或者更低点，但没想到DNA的效率这么低
。还是我实验出了问题，
请问有什么步骤是需要优化的吗？
谢谢toll和各位。 |
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v*******y
发帖数: 5530 | 3 my best guess is that he is talking about calculating a spot rate given a
yield curve
otherwise, I don't know what he is talking about either |
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f*******r
发帖数: 383 | 4 Can anyone here kind enough to help me find the following paper?
Please kindly send the paper to c*********[email protected].
Thanks a million.
Fitting yields curves and forward curves with maximum smoothness.
K.J. Adams and D.R. Van Deventer.
The Journal of Fixed Income, 4(1):52-62, June 1994. |
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n******a
发帖数: 288 | 5 昨天上午七点四十，在我系的一条街道上我的车被学校的警察拦下，他问我有没有看到
刚才有一个行人在人行道上我没有yield。我说yeah(当时很蒙），他当时很凶。后来我
说我是等我pass那个行人时才看到的，他就说我撒谎，说我刚开始承认看到了。。。他
给我开了罚单，叫我下周四上庭。他说我可能会罚180美金。还会有记录。真倒霉。来
美国一年左右，自己以前从来没开过车，拿到驾照才2个月。 |
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I**C
发帖数: 100 | 6 我买了二手的high yield 的解剖和神经解剖，是不是很薄的啊，
看了不少前辈的介绍，说找个比kaplan的解剖好，那还要不要看kaplan 呢，谢谢！ |
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L***N
发帖数: 429 | 7 新手上路，找东西找的好晕啊，哪位可以给个下载
high yield 的书 和 Goljan audio transcript/slides 的link吗？
或者发我信箱里？
非常感谢！！！ |
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I*****n
发帖数: 47 | 8 请问谁有High-Yield Gross Anatomy 电子版或者下载的链接啊！可否传一份给我，多
谢！！！
email: x***[email protected] |
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n********m
发帖数: 37 | 9 Dear XDZM,
We are beginning a "High-Yield Neuroanatomy review Group" this week. Will
meet 2 hours / day, Mon to Fri, use go-to-meeting and dropbox to meet /
present and share.
We have 4-6 person in the group who all finished 1-2 round of FA, and part
of the UW bank.
I knew a lot of you had good previous experience with HY-NA group study,
would you please give us some good suggestions on how to make this group HY-
NA study most efficient?
Our goal is to make the group study
1. The best underst... 阅读全帖 |
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b*****t
发帖数: 64 | 10 需step 2 ck high yield,就是前面有人贴的CK MTB video， mtb2配套的。 谁有全套
，可以考虑交换类似价值资料。 |
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l******o
发帖数: 42 | 11 寻找 High-yield neuroanatomy 学习小组。或者有志同道合者也可一起组建个小组。
有兴趣者请留下skype id。 |
|
l******o
发帖数: 42 | 12 我们将用 High Yield - Neuroanatomy 4th edition 为参考书。如果需要电子版请给
我信到站内信箱。 |
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F*******I
发帖数: 200 | 13 谢谢组织这个学习小组。有些小疑惑：除了High Yield - Neuroanatomy 4th edition
， 是否考虑其他学习资料呢？ |
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M********0
发帖数: 62 | 14 我们小组现在已经有3人，现在希望再招两位童鞋一起过high yield neuroanatomy这本
书。
预计是两周过完，每周skype见面5次， 每次两小时左右，强度比较大。我们组员的大
概进度是过了fa 和题库，希望招进度类似的童鞋。
有兴趣的话请回站内信。多谢！ |
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c**********e
发帖数: 2007 | 15 Does anybody know how to calculate Real Yield to Maturity for a Treasury
Inflation Protected Bond?
It is a surprise that it is hard to find the formula. |
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p********0
发帖数: 186 | 16 Hi,
I have one question about getting the yield date based on bond price.
Assume today is 11/05/2011
Continuous compounding convention, discount factor is e^{-tr(t)}
We have the bond quotes on Nov 5, 2011
Coupon Maturity Ask price
6.625 2/15/2012 100
9.125 2/15/2012 100.6875
7.875 8/15/2012 100.75
8.25 8/15/2012 101.03125
I am using the term structure formula
PV(present value of asking price) 100 = x_{0}(cash flow} + d_{1} * x_{1} (
100+6.625*0 |
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p********0
发帖数: 186 | 17 Sorry, typo, it should be yield rate, not date |
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n*****r
发帖数: 159 | 18 The quoted price is clean price, but you have to use dirty price (clean
price + accrued interest) in your equation because you have to pay more than
100 to get 6.625, 2/15/2012 bond.
Simply think that you can get a 6.625/2 coupon after holding a bond for 3
months, you will get 6.625*2 >12% annualized yield rate, which is of course
ridiculous. |
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p********0
发帖数: 186 | 19 Thanks for the reply,
I am a novice, If I am understanding correctly, you paid the bond asking
price,
then you will get coupon + principal back at the maturity date.
11/05/2011 is between two coupon date, so I proportionally get the coupon
100/182*6.625.
What's the correct way to get the yield rate/interest rate using the data
above? |
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c******n
发帖数: 1 | 20
at
the
So from Nov 5 to Feb 15 is 100 days, assuming bond yield rate y, we will
have
($100 + 6.625*(180-100)/360) = exp(y*100/360)*($100+6.625*0.5)
this gives y = 6.4704%
whereas the second one gives:
($100.6875 + 9.125*(180-100)/360) = exp(y*100/360)*($100+9.125*0.5)
which gives y = 6.4167%
Should we expect this to be the same, or that's close enough? |
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r**u
发帖数: 69 | 21 flight to quality.
yield？
|
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i********y
发帖数: 346 | 22 觉得这个定义和股票显示的怎么不一致啊？比如citi group，dividend 0.16, yield
16.98, one share price 3.77. 但是0.16/3.77*100=4.244 !=16.98啊？怎么回事呢？ |
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A***l
发帖数: 302 | 23 dividend is quarterly; yield is yearly. |
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k*n
发帖数: 108 | 24 effdur by mv weighted such that portfolio yield doesn't change portfolio mv. |
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h**********k
发帖数: 168 | 25 you have to define the yield first. |
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m*******n
发帖数: 154 | 26 行外人，偶然要用这个数据。要monthly data on 5-year US T-Bond yields (plain
vanilla, coupon bonds) after December 1994。求问哪里可以找到，如果不麻烦是否
可帮忙找到发到我邮箱，不胜感激！！！i*******[email protected] |
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s*********1
发帖数: 34 | 27 比如说，30年的yield变小，1年的变大，可以long 30年的，short 1年的赚钱么？ |
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P*********t
发帖数: 4451 | 28 我用中文讲吧
yield 就是“需要用的“弹跳力
窈窕的墙是100厘米
你的身高就是价钱
全班每个同学都跳的过去
矮点的同学得多用点弹跳力。
高点的同学只要一点点用力就过了。 |
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x**y
发帖数: 10012 | 29 他的问题是分不清楚yield 和interest rate的关系 |
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t***s
发帖数: 4666 | 30 you're not buying yield, you're buying cash flow. |
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t********a
发帖数: 810 | 31 Higher yield means you receive more discount when buying the underlying
cashflow. The more the discount, the lower the price. |
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o********n
发帖数: 100 | 32 yield好比性价比.
你某时刻买入bond, 它给你的总的现金流是衡定值(因为coupon和face value一定).因
此付越少的钱性价比越高.
说得清楚吗? |
|
s***r
发帖数: 1121 | 33 Yield is your required rate of return. You require more, so the price is
lower. |
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y********e
发帖数: 8315 | 34 yield越高
pv越低
所以价格就越低
理论上来讲market return基本是一致的 |
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w******g
发帖数: 271 | 35 Hi, Da Ge Da Jie,
Did someone read "Understanding the yield curve" by Salomon Brothers before?
I need someone to teach me on this, don't worry, I have finance background.
I am PAYING to who can walk me through these 5 lectures within a short time. |
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i*******1
发帖数: 14 | 36 现在什么方法比较主流？macro economic variables + some sort of regression
model? 更关心的是10 year treasury yield的变化趋势，即trending,but less about
its absolute level。不知道有什么比较好的办法没有？ |
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p******i
发帖数: 1358 | 37 那阵子treasury的yield就是负的，您老没读书看报？
price |
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k*******d
发帖数: 1340 | 38 计算Bond的Yield to Maturity，感觉应该没有analytical的方法把，除了Newton法来
求解以外，还有什么快速的方法没？遇到过一个问题是如果一个晚上要算完10,000个
bond的YTM,怎么算比较快。 |
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m**h
发帖数: 4 | 39 Does any one know how to estimate the discount rate or yield curve in the
loan portfolio valuation given prepay, default, and recovery rate are known?
Thanks. |
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l******i
发帖数: 104 | 40 对这个Yield Book不太了解，好像主要搞fixed income的model的，在学校career
center乱投的，没想到给了电面，说是3个人，一小时左右。请问有没有谁和他们面过
，都问什么类型的问题啊？这是我目前第一个quant的正式面试，没有经验，希望大家
能多指教。多谢了！ |
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o********4
发帖数: 45 | 41 請問一下, 以下的bond yield equation中, 當N(年份)是單數是, 相應的i只
有一個, 但當N是雙數是, 相應的i就有2個了, 一個正,一個負, 那負的那個是什麼意思
呢?
因為本人在用Newton method 找i, 但發覺有當Ｎ＝ＥＶＥＮ時出現２個ＲＯＯＴＳ。 |
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D********n
发帖数: 978 | 42 嗯，construct出来的yield curve被调用500次以上，如果赔钱，modeler可能被判刑。 |
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a*****c
发帖数: 3525 | 43 如果你做过，跟什么东西比较？怎么测？有文献吗？能否给指点一下？ 谢谢！
合成了一些东西，带荧光，想测一下quantum yield. |
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l******o
发帖数: 162 | 44 I am using SAS 9.1. Why does the log say that "the yield function is unknow or
cannot be accessed".
Thanks. |
|
w*******y
发帖数: 60932 | 45 8 Pack Brother LC61 (LC-61) High Yield Compatible Inkjet/Ink Cartridges
Combo (Two each: LC61BK, LC61C, LC61M, LC61Y) for $7.39 Free Shipping!
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|
w*******y
发帖数: 60932 | 46 8 Pack Brother LC61 (LC-61) High Yield Compatible Inkjet/Ink Cartridges
Combo (Two each: LC61BK, LC61C, LC61M, LC61Y)
Use Promo Code: YRETX3
Supported Models
BROTHER DCP 165C
BROTHER MFC 250C
BROTHER MFC 255CW
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BROTHER MFC 295CN
BROTHER DCP 375CW
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w*******y
发帖数: 60932 | 50 Features
*Dishwasher-safe nonstick inner cooking pot, makes 6-cups of cooked rice
*Stay-cool lid handle and side handles, "cook" and "warm" indicator lights
*Tempered glass lid with steam vent; automatic keep-warm mode
*Includes measuring cup, rice paddle, and plastic steamer basket
*Measures approximately 8-2/3 by 8-2/3 by 7-2/5 inches
Link:
http://www.amazon.com/Black-Decker-Uncooked-Yields-Cooked/dp/B0
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