g*****n
发帖数: 241 | 1 我替我们系的一个朋友问个问题，他想在zebrafish的上皮细胞特异性的表达GFP，但他
不研究zebrafish，所以不知道该到哪里找相关的zebrafish的promoter的资料。请研究
zebrafish的同僚帮忙指点一下。
谢谢 |
|
x***m
发帖数: 184 | 2 最近也对zebrafish感兴趣，可惜周围都没有实验室可以交流。
请问现在大家用zebrafish做模式生物的研究热点有那些? 较之于其它vertebrate模式
生物如frog, chicken和mice有那些特别的优势呢, 除了比较透明?
美国有哪些做zebrafish的好实验室？ |
|
x***m
发帖数: 184 | 3 为什么在frog和chicken里面做genetic screen但在zebrafish里可以呢? 记得很多年前
就读到过用EMS突变老鼠进行genetics screen啊.
记得zebrafish也有genome duplication, 所以在downregulate gene expression上很
依赖morpholino. |
|
s******y
发帖数: 137 | 4 其实从transgenetic 和genetic screen来说zebrafish未必及的上medaka（是一种东亚
特有的鱼类），zebrafish的基因组测序的质量也一般吧。历史上主要由于其鱼卵子互
相不粘连比较适合观察不同发育时期而广泛使用。 |
|
b*********l
发帖数: 490 | 5 I am thinking another issue. During the process of zebrafish like fixation-
dehydration-rehydration, all of the MeOH+PBT solution should be with DEPC
treated water or what? Is it possible that the mRNAs in zebrafish have been
deteriated before the hybridizaion? I am not sure how bad the
invivo mRNA will be if I don't use RNase free reagents. The thing is I
couldn't see any difference between negative control and the real samples.
Thank you for any suggestions. |
|
X******n
发帖数: 914 | 6 想换个系统做做，大家觉得zebrafish找个系统怎么样？比如说和线虫，酵母比较，有
什么优缺点？ |
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p*****m
发帖数: 7030 | 7 He is a student of Shreiber, and postdoc of Mark Fishman. SO it is not
surprising he combined chemical screen with zebrafish:) |
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y***i
发帖数: 11639 | 8 Yeah, but not nearly as easy as with fly worm and zebrafish.
in |
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y***i
发帖数: 11639 | 9 No KI? Transgenic zebrafish are common right?
.. |
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O******e
发帖数: 4845 | 10 忍不住了哈。你到底对哪个specific question感兴趣，非得zebrafish啊？干嘛不
迎着困难直接小鼠得了？
Sorry, I'm a mouse person, hoho. |
|
a********k
发帖数: 2273 | 11 俺琢磨着fish也就剩显微镜这一个胡同了，不过larval zebrafish很难做疾病模型，而
adult其实也不是很好看。希望有些发育方面的疾病可以用这个做chemical筛选吧。 |
|
a********k
发帖数: 2273 | 12 medaka不是挺难inject么，有厚厚的卵膜。基因组是zebrafish最大的障碍，基本没有
可能override的。 |
|
M*****n
发帖数: 16729 | 13 not possible.
we have many fish facilities on campus and that department has at least
three faculty studying zebrafish, as I know.
i think only relatively smaller universities will have that kind of concern.
for bigger unversities, investing for a fish facility is not a big deal.
btw, for that mm, if she wants to kick off her proposed project, she does not need a big facility, besides, my feeling is she is going to migrate to mouse genetics. |
|
m****9
发帖数: 128 | 14 We submitted a paper to Cell and got response, the reviewer asked for in
vivo study data(in zebrafish,xenopus or drosophila)
We identified a tumor suppressor via activating TGF-b signaling, the
reviewer wanted to know how it affects tgf-b signaling in vivo.
Can anyone give some information about this or recommend some nice guys that
we can cooperate with? It is better in Boston area.
Thanks for help. |
|
w***y
发帖数: 493 | 15 我把3xHA的tag接到蛋白的N端, 然后做了in vitro transcription得到了mRNA. 接着把
mRNA注射到了鱼的卵里,另外准备了一些uninjected fish作为negative control, 一段
时间后做western观察蛋白表达情况. 我发现在那些negative control的样品里也有带,
而且量还不少, 大概在75KD 左右. 我试过两个不同的 anti-HA的抗体, 结果都一样.
请问有人在zebrafish里面表达过HA-tag的蛋白么, 有没有看到这种情况呢? |
|
s********8
发帖数: 619 | 16 你的意思是IRES更reliable吗? 刚才研究了下,好像说IRES的表达不能保证两个蛋白的
比例是1:1,而且zebrafish里面这个IRES的活性比较低,不知道是不是这样?
请大家多提意见吧,现在还没有决定到底用那个. |
|
a****o
发帖数: 1786 | 17 【 以下文字转载自 Biology 讨论区 】
发信人: Morphin (莫非-白衣“追鱼”郎), 信区: Biology
标 题: zebrafish/imaging job posting
发信站: BBS 未名空间站 (Thu Nov 5 17:34:24 2009, 美东)
ASSISTANT RESEARCH PROFESSOR
INDIANA UNIVERSITY-PURDUE UNIVERSITY INDIANAPOLIS
The Indiana University School of Medicine
Department of Medicine, Division of Nephrology
The IU School of Medicine invites applications for Assistant Research
Professor or above, appointment type and rank to be determined by
qualifications and interest.
The Division of Nephr |
|
p*******l
发帖数: 10 | 18 千人彭金荣担任动物科学学院和生命科学学院两个院长, 从2008年回国后发表的SCI论
文（说明：IF标注的是2013的），包括综述。
1: Zhu Z, Chen J, Xiong JW, Peng J. Haploinsufficiency of Def activates p53-
dependent TGFβ signalling and causes scar formation after partial
hepatectomy. PLoS One. 2014 May 6;9(5):e96576. （IF=3.534）
2: Ou Z, Yin L, Chang C, Peng J, Chen J. Protein interaction between p53 and
Δ113p53 is required for the anti-apoptotic function of Δ113p53. J Genet
Genomics. 2014 Feb 20;41(2):53-62.
(IF=2.924)
3: Tao T, Shi H, Huang D, Peng J. Def... 阅读全帖 |
|
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M*****n
发帖数: 16729 | 20 Our friend and colleague Chi-Bin Chien died December 2, 2011, his battle
with cancer over. As many of you know, Chi-Bin defied his disease with
courage and optimism for over 12 years. During this period Chi-Bin
emerged as a leader in our field: He helped organize and lead our
international meetings, served as Director of the Zebrafish Neural
Development course at Woods Hole, cheerfully handed out new tools and
reagents his lab developed to help accelerate work with zebrafish,
brought us insights... 阅读全帖 |
|
G*P
发帖数: 214 | 21 不过第一段英语写得真差，之前如果完全不知道此事的话，这段话可以让你云里雾里，
而且混淆当事人
http://www.nature.com/news/2011/110608/full/474140a.html
Whistle-blower claims his accusations cost him his job
University denies it retaliated against researcher who questioned supervisor
's data.
Eugenie Samuel Reich
Zebrafish embryos are at the centre of a dispute over research integrity.
Zebrafish embryos are at the centre of a dispute over research integrity.R.
KESSEL/VISUALS UNLIMITED/CORBIS
After months of friction that culminated in his open... 阅读全帖 |
|
l**********1
发帖数: 5204 | 22 You sure?
Heine, V. M. et al. (2011)
Small-molecule Smoothened agonist prevents glucocorticoid-induced neonatal
cerebellar injury.
Science Transl. Med. 3: 105ra104
Firestone, A. J. and Chen, J. K. (2010)
Controlling destiny through chemistry: Small-molecule regulators of cell
fate.
ACS Chem. Biol. 15: 15-34.
http://chen.stanford.edu/index.php?option=com_content&view=arti
plus
Zebrafish Protocols
Bleaching Zebrafish Embryos
Genomic DNA Isolation for PCR Analysis
Manual and Enzymatic Dechorionat... 阅读全帖 |
|
c********n
发帖数: 67 | 23 The Evaluation of Rapid Cooling As an Anesthetic Method for the Zebrafish.
PMID: 24093489
Effect of the Dietary Inclusion of Soybean Components on the Innate Immune
System in Zebrafish
PMID: 24392798
Low-Cost Silicone Imaging Casts for Zebrafish Embryos and Larvae
PMID: 24237049
Impact of Maternal Nicotine Exposure on Expression of Myelin-Related Genes
in Zebrafish Larvae
PMID: 24206014
Thanks very much for your help |
|
t*d
发帖数: 1290 | 24 我们是一直在寻找更好的 model。比如如果有 validated 孤儿老鼠的 model，比我们
现在用的 single gene mutant model 好，为什么不用呢？基本上所有药厂都会找到性
价比最高/次高、最好的、最稳定的 models 来用。问题是好 model 实在太少了。一个
新的 model 的 validation 过程是很漫长，很漫长，因为需要多个 clinic trials 的
验证。这也导致旧的 validated 的 model 不容易被轻易放弃。
基于zebrafish 的screen 对人类的 safety 有指导意义吗？因为 in vitro 筛出来的
药都是药做耗子、狗狗等毒性实验的。如果耗子、狗狗上发现不了毒性，zebrafish 上
发现的毒性有多大意义呢？
另外，当我们讨论 false positive 的时候，也需要考虑 false negative。很多人相
信有些药在 R&D 阶段过早地被抛弃了。zebrafish 等和人差这么老远，基于他的毒性
而否定一个药，很多领导是不干的。
validity
性。
对于 |
|
m****s
发帖数: 18160 | 25 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position Available in Cancer/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:21:47 2013, 美东)
Post-doc Position Available in Cancer/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF-β signal transduction in cancer.
Focus of the res... 阅读全帖 |
|
m****s
发帖数: 18160 | 26 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position Available in Cancer/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:21:47 2013, 美东)
Post-doc Position Available in Cancer/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF-β signal transduction in cancer.
Focus of the res... 阅读全帖 |
|
|
m**********3
发帖数: 706 | 28 He received his postdoc training from Harvard. And he had some decent
publications. His level should be equal to some AP in the US.
Liu TX, Becker MW, Jelinek J, Wu HS, Deng M, Hsu K, Bloomfield C, Stone R,
Issa J-P, Erba HP, Clarke MF, Look AT. (2006) Deletion del(5q) and
epigenetic suppression of alpha-catenin (CTNNA1) in myeloid malignancy.
Nature Medicine.
Liu TX, Howlett NG, Deng M, Langenau DM, Hsu K, Rhodes J, Kanki JP, D'Andrea
AD, Look AT. (2003) Disruption of FANCD2 function causes con... 阅读全帖 |
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a*****x
发帖数: 901 | 29 Zebrafish is actually must less hot than a few years ago. There are a few
really promising drugs in clinical trials very recently from zebrafish
screenings in FATE pharmaceuticals though |
|
c********n
发帖数: 67 | 30 Isolation and phenogenetics of a novel circadian rhythm mutant in zebrafish
Identification of a mutation in the Clock1 gene affecting zebrafish circadia
Thanks very much for your kindly help
h***[email protected] |
|
l**********1
发帖数: 5204 | 31 CY Ms? did zebrafish PKD zebrafish model?
her one PNAS paper link:
//www.ncbi.nlm.nih.gov/pubmed/19966229 |
|
c********n
发帖数: 67 | 32 Feeding entrainment of food-anticipatory activity and per1 expression in the
brain and liver of zebrafish under different lighting and feeding
conditions.
Feeding entrainment of daily rhythms of locomotor activity and clock gene
expression in zebrafish brain
Thanks very much for you kindly help
h***[email protected] |
|
s******9
发帖数: 283 | 33 人说：
"If this takes away from any of the R01s that would normally be funded by
the NIH, it would be bad. Right now the community is already so strapped we'
re at a breaking point."
called several of its technological proposals "science fiction"
...
http://www.sciencemag.org/content/339/6123/1022.full
Brain Project Draws Presidential Interest, but Mixed Reactions
Emily Underwood*
Shortly after President Barack Obama made a seemingly innocuous pitch for
more brain research in his State of the Union... 阅读全帖 |
|
A****A
发帖数: 16 | 34 物种不同难度也不同。TALEN自己做不贵。
可以参考最近的Zebrafish的文章
Nature Methods | Brief Communication
TALEN-mediated precise genome modification by homologous recombination in
zebrafish
Nature Methods
(2013)
doi:10.1038/nmeth.2374 |
|
A****A
发帖数: 16 | 35 物种不同难度也不同。TALEN自己做不贵。
可以参考最近的Zebrafish的文章
Nature Methods | Brief Communication
TALEN-mediated precise genome modification by homologous recombination in
zebrafish
Nature Methods
(2013)
doi:10.1038/nmeth.2374 |
|
s******9
发帖数: 283 | 36 http://www.sciencemag.org/content/early/2013/03/06/science.1236
The Brain Activity Map
A. Paul Alivisatos1,*,
Miyoung Chun2,
George M. Church3,
Karl Deisseroth4,
John P. Donoghue5,
Ralph J. Greenspan6,
Paul L. McEuen7,
Michael L. Roukes8,
Terrence J. Sejnowski9,*,
Paul S. Weiss10,
Rafael Yuste11,*
Abstract
Researchers propose building technologies to enable comprehensive mapping
and control of neural circuit activity.
Neuroscientists have made impressive advances in understanding the
microscale ... 阅读全帖 |
|
a********k
发帖数: 2273 | 37 cell封面文章。犹他HHMI Cairns和中科院刘江的背靠背文章，斑马鱼精子甲基化可以
reprogram卵子基因组。
Reprogramming the Maternal Zebrafish Genome after Fertilization to Match
the Paternal Methylation Pattern p759
Magdalena E. Potok, David A. Nix, Timothy J. Parnell, Bradley R. Cairns
Sperm, but Not Oocyte, DNA Methylome Is Inherited by Zebrafish Early Embryos
Lan Jiang, Jing Zhang, Jing-Jing Wang, Lu Wang, Li Zhang, Guoqiang Li,
Xiaodan
Yang, Xin Ma, Xin Sun, Jun Cai, Jun Zhang, Xingxu Huang, Miao Yu, Xuegeng
Wang, Feng Liu, Chung-I W... 阅读全帖 |
|
S******i
发帖数: 71 | 38 应该把自己的出生告诉大家，不要空对空，要留下学校的阳西地址，还有最好把系主任
的联系方式也放这里
Guo Huang Ph.D.
Assistant Professor
CVRI Investigator
Website
Research Interests:
Comparative study of heart development and regeneration, ischemic heart
diseases, stem cell, cardiomyocyte proliferation, regenerative biology
Program Areas
Vascular Biology and Atherothrombosis
Developmental Biology and Congenital Anomalies
Channels and Arrhythmias
Muscle Biology and Heart Failure
Prediction and Prevention of Cardiovascular Disease
Research Summary
... 阅读全帖 |
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c********n
发帖数: 225 | 39 【记录历史】谁重复验证了NgAgo？
项目 结题
date： 2017年8月2日
updated: 2017年8月4日
Note2017080401: added a few notable reviews/summaries
---------------------------------------
- NBT retraction note：
http://www.nature.com/nbt/journal/v34/n7/full/nbt.3547.html#correction2
原文引用
“Retracted online 02 August 2017
We are retracting our study because of the continued inability of the
research community to replicate the key results in Figure 4, using the
protocols provided in our paper. In this figure we report that the
Natro... 阅读全帖 |
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m******5
发帖数: 13 | 40 zebrafish是合作组做的，我主要就是负责建预测模型，之后预测的结果在zebrafish上
验证。
并不是感觉学术界可怕，只是个人倾向工业界。
不过很谢谢你的意见了 |
|
f**d
发帖数: 768 | 41 【 以下文字转载自 Biology 讨论区 】
发信人: synbio79 (jingzhuo), 信区: Biology
标 题: Brain Activity Map: 一群人联合起来了
发信站: BBS 未名空间站 (Thu Mar 7 22:38:02 2013, 美东)
http://www.sciencemag.org/content/early/2013/03/06/science.1236
The Brain Activity Map
A. Paul Alivisatos1,*,
Miyoung Chun2,
George M. Church3,
Karl Deisseroth4,
John P. Donoghue5,
Ralph J. Greenspan6,
Paul L. McEuen7,
Michael L. Roukes8,
Terrence J. Sejnowski9,*,
Paul S. Weiss10,
Rafael Yuste11,*
Abstract
Researchers propose building technologies to enab... 阅读全帖 |
|
l*******e
发帖数: 358 | 42 其实转基因究竟有害还是无害，至少目前没有结论
至于人吃的东西是否健康，要完全按照西方科学方法论去研究的话，所有的东西都有有
害成分，这个如何定论
再者比如吃药，吃药可以治病，但是能说吃药没有毒性么？
最简单，目前全世界通用的退热药，扑热息痛，婴儿亦可食用，但在毒理测试的时候四
天10mg处理就可以把adult zebrafish 毒死
那么究竟什么是有毒，什么是无毒
说来说去只是没有一个完善的标准，而一味的攻击转基因的安全性是否偏颇？
40- |
|
F*******n
发帖数: 813 | 43 希望以后，你的帖子会扼杀在我的摇篮。。。
不过 ，我要走了。。。
你爱怎么发就这么发 了
他们说你是zb..
我不知道啥缩写
ZB 可以是一种鱼的缩写。。。中文缩写。。ZEBRAFISH..
BUT THE REAL NAME FOR THAT FISH IS
Danio Rerio
this is called terminology |
|
发帖数: 1 | 44 @All
Make America GREAT again . 你们滚回中国，if you keep 人在曹营心在汉！love my
America!!!!!!
各个业界的朋友，尤其是在海外的，如果我对行情分析有误，一定要更正我。我的二次
创业再也不能利用信息不对等而从中谋利：那不是创业，那个叫二道贩子。
我们的创业要为社会创造价值，让世界更得加美好。
我个人认为：生意人混的就是一个信誉。我们应该象珍惜自己的生命一样珍惜自己的名
字，自己公司的信誉，自己公司的售后服务。
祝大家鸡年多生蛋：千万不能杀鸡取卵！
昨天你们都干什么了？取得了什么进展？
Danio 是鱼的意思，取自我的博士课题：Danio Rerio, zebrafish..
我儿子从daycare学会的第一句话：big fish eat little fish..。
这是我的第二个公司，第一个公司是以我的两个孩子的名字命名，己经解体了。。所以
，我要把这个坚持下去。。Donal Trump 不是破产5个吗？失败没有什么了不起。
Just do it!!! |
|
l**********i
发帖数: 11748 | 45 我擦 钓上来个机器人
[在 CornucopiaX (ThanksGiving) 的大作中提到：]
：欢迎您加入“鱼会微信群”！此群是受国际狮子会的启示，想成立一个相似的组织。
因为我博士期间是研究zebrafish的，所以以此命名。https://zh.m.wikipedia.org/zh
/国际狮子会 前方的路程是艰难的，朋友们，加
：油！想入“鱼会”的同学请告诉我！我可以给你barcode to scan .目前“鱼会微信群
：........... |
|
m****s
发帖数: 18160 | 46 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position in Vascular Biology/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:22:51 2013, 美东)
Post-doc Position in Vascular Biology/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF- signal transduction in cardiovascular biolog... 阅读全帖 |
|
N*********6
发帖数: 2302 | 47 卵巢癌会影响大脑皮层？？天下奇闻啊。。你对一个去逝的人能尊重一些么？
我博士就是研究zebrafish,,, 说我养魚的脑袋，you got it...我还养青蛙，老鼠。。
我是学生物的笨蛋，没有你聪明，。。我后来至少2年和她没有任何往来，你的包裹污
蔑我之事，我一辈子都忘不了。。
我喷什么了。。
我是死笨，上帝是可怜我，今天有机会让大家观看一下我有多笨。。 |
|
发帖数: 1 | 48 欢迎您加入“鱼会微信群”！此群是受国际狮子会的启示，想成立一个相似的组织。因
为我博士期间是研究zebrafish的，所以以此命名。https://zh.m.wikipedia.org/zh/%
E5%9B%BD%E9%99%85%E7%8B%AE%E5%AD%90%E4%BC%9A 前方的路程是艰难的，朋友们，加
油！想入“鱼会”的同学请告诉我！我可以给你barcode to scan .目前“鱼会微信群
”已有60人。 |
|
M*****n
发帖数: 16729 | 49 OCT，
上次看一nature method paper, 用这个看drosophila embryos,和zebrafish embryo
俺老板说要买这个，结果俺journal club这个paper， 比较一下跟confocal 和two-
photon.
老板就觉得OCT没啥特别好了。 |
|
B*M
发帖数: 1418 | 50 SCIENTIFIC MISCONDUCT
Sabotaged Scientist Sues Yale and Her Lab Chief
Martin Enserink
When Magdalena Koziol suspected that someone was sabotaging her research at
Yale University, she did what comes naturally to a scientist: She set up a
controlled experiment to test her hypothesis.
Koziol's studies of how the genome switches on after an egg is fertilized
had begun failing mysteriously in July 2011, a month after she started her
postdoc in the developmental biology lab of Antonio Giraldez. In Aug... 阅读全帖 |
|
