p*****n
发帖数: 981 | 1 ☆─────────────────────────────────────☆
bewithu (x-fire in the sea) 于 (Sun Dec 31 10:38:41 2006) 提到:
For regular immunofluorescence, Trition may be good enough (such as 0.1% in
PBS, 10-15 min, RT). Saponin, which is generally purified from plants, is a
much weaker detergent. It is believed that the permeabilzation effect given
by saponin is reversible, thus you need to use saponin-containing solutions
for all steps during IF (Ab incubation and washing).
The advantage is that saponin will gi |
|
