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Biology版 - [合集] How to increase washing stringency in IP?
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相关话题的讨论汇总
话题: ip话题: stringency话题: washing话题: increase话题: fixation
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chick (Por Los Momentos Dif Ciles) 于 (Wed Jan 3 13:21:18 2007) 提到:
I'm working on the project involved something similar to Ch-IP. Recently, I
got very weird result, in some group or controls, the Non-fixation IP has
even stronger singal than the formaldehyde fixation. This could partialy be
explained by the over-crosslinking lowered the possible detecable targets.
But, obviously, I need more stringent washing condition, at least to
diminish the non-
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Re: northern blotting problemChIP的问题
Re: 有用anti-GFP Ab做肿瘤组织染色的吗?question about the EM
大侠们做chip是用sonicator还是用MNase digest?问一下methanol/acetic acid的fixation
NGS_Illumina类能不能用可逆的 fixation 做 RPPA?
相关话题的讨论汇总
话题: ip话题: stringency话题: washing话题: increase话题: fixation