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发帖数: 27 | 1 For your question of slow growth, i suggest you set some controls. first of
all, clean the bottles, get rid of all possible remaining detergent. make
fresh media yourself, pay attention to the salt concentration/pH. while
autoclave, only 15min. when you culture the cell, never let the volume take
too much of the bottle. make sure there is enough O2 supply. since it harbors
a plasmid that has resistance to two different antiboitics, maybe the growth
pressure make it grow very slowly. so set a con |
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