M****e
发帖数: 70 | 1 this is a BIG problem. so what is the gene? and from what
kind of organism would u like to clone it? i have only a
couple of suggestions. first of all, check the RACE primers.
have you tried several different primers? 2nd, check the
known EST database and see whether there are severa image
clones available with which you can actually assemble the
clone. this gene might have very long 5'UTR. you may not
need the whole sequence for expression, though the UTR may
have regulatory sequences. 3rd, you |
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