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发帖数: 70 | 1 the essential step is to keep everything as clean as possible.
and do not use anything you are suspicious. Ambion is a good
company that has very good experience dealing with RNA. my
personal experience is that: ALWAYS aliquot reagents. i used
to use DEPC'd ddH2O, but now i would rather use commercial
nuclease free water. try to be a quick and clean hand, quick
and dead, you know that? i.e., you quick and RNase dead, no
no vice versa. when you deal with different biological samples,
you have dif |
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