s***l
发帖数: 42 | 1 This is what i would do if i were you:
1. Check if the 2nd ab cross-react with the species of your tissue sample
2. Use cold acetone to permealize and fix the tissue in step2
3. decrease the 1st ab concentration,and try O/N incubation in 4 degree
4, mix 2 2nd ab and spin at max speed for 2min. Sometimes, Texas red and FITC
form small particle precipitates.
I think the wash is enough in your protocol.
Good luck
谢 |
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