l*****k
发帖数: 587 | 1 let me tell you the best way ( I think ):
preabsorption
which means you first incubate your 2nd antibody with your control for several
hours, then use the pre-incubated antibody on your real sample.
it usually gives great results.
hope this helps. | l*****k
发帖数: 587 | 2 I personally think it really depends on your sample, I think shaking
is not going to help in any way... however, I never risked not shaking.
for your particular case, I would say it may be fine if you don't shake--
if it causes your cell to get lost from the slide.
again, you probably need to test it yourself, like in your next
experiment, include shaking and not shaking, see what difference
does it make.
good luck |
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