s******y
发帖数: 28562 | 1 I am preparing a low yield plasmid and I need a lot of it.
I want to use the chrolamphenicol but I forgot the protocol because I have
not done it for a long, long time.
I roughly remember I should add the chrolamphenicol to the overnight culture
and then continue for some time, but I forgot what concentration of
chrolamphenicol I should use and how long I should incubate the bacteria
with the chrolamphenicol.
Any info would be appreciated. Thanks in advance! | p****p
发帖数: 3360 | 2 Check out the Maxi Prep protocol from Sambrook's Molecular Cloning. If I
remember correctly, it should be 170 ug/ml final O/N.
culture
【在 s******y 的大作中提到】
: I am preparing a low yield plasmid and I need a lot of it.
: I want to use the chrolamphenicol but I forgot the protocol because I have
: not done it for a long, long time.
: I roughly remember I should add the chrolamphenicol to the overnight culture
: and then continue for some time, but I forgot what concentration of
: chrolamphenicol I should use and how long I should incubate the bacteria
: with the chrolamphenicol.
: Any info would be appreciated. Thanks in advance!
| s******y
发帖数: 28562 | 3 Thank you!
I got it
,
【在 p****p 的大作中提到】
: Check out the Maxi Prep protocol from Sambrook's Molecular Cloning. If I
: remember correctly, it should be 170 ug/ml final O/N.
:
: culture
|
|
