h***9 发帖数: 662 | 1 最后dehydration,在100% ethanol里面(也就是放到xylene之前那一步),最长可以泡
多久?谢谢。 |
s******y 发帖数: 28562 | 2 If you do it in the room air (instead of low pressure chamber) then you can
leave it overnight in a consealed chamber.
【在 h***9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最后dehydration,在100% ethanol里面(也就是放到xylene之前那一步),最长可以泡 : 多久?谢谢。
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s******y 发帖数: 28562 | 3 If you are talking about the maximum time?
For whole embryo, sometimes we keep them in 100% ethanol for a long long
time like several month. But for slides I don't know, because we never keep
them for more than 24hours so I cannot answer that.
【在 h***9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最后dehydration,在100% ethanol里面(也就是放到xylene之前那一步),最长可以泡 : 多久?谢谢。
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h***9 发帖数: 662 | 4 thank you. they are tissue slides. I left them in 100% ethanol for two hours
. they seemed to be fine.
keep
【在 s******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : If you are talking about the maximum time? : For whole embryo, sometimes we keep them in 100% ethanol for a long long : time like several month. But for slides I don't know, because we never keep : them for more than 24hours so I cannot answer that.
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s******y 发帖数: 28562 | 5 two hours is nothing.
hours
【在 h***9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : thank you. they are tissue slides. I left them in 100% ethanol for two hours : . they seemed to be fine. : : keep
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D******9 发帖数: 2665 | |