h***9
发帖数: 662 | 1 最后dehydration，在100% ethanol里面（也就是放到xylene之前那一步）,最长可以泡
多久？谢谢。 |
s******y
发帖数: 28562 | 2 If you do it in the room air (instead of low pressure chamber) then you can
leave it overnight in a consealed chamber.
【在 h***9 的大作中提到】
: 最后dehydration，在100% ethanol里面（也就是放到xylene之前那一步）,最长可以泡
: 多久？谢谢。
|
s******y
发帖数: 28562 | 3 If you are talking about the maximum time?
For whole embryo, sometimes we keep them in 100% ethanol for a long long
time like several month. But for slides I don't know, because we never keep
them for more than 24hours so I cannot answer that.
【在 h***9 的大作中提到】
: 最后dehydration，在100% ethanol里面（也就是放到xylene之前那一步）,最长可以泡
: 多久？谢谢。
|
h***9
发帖数: 662 | 4 thank you. they are tissue slides. I left them in 100% ethanol for two hours
. they seemed to be fine.
keep
【在 s******y 的大作中提到】
: If you are talking about the maximum time?
: For whole embryo, sometimes we keep them in 100% ethanol for a long long
: time like several month. But for slides I don't know, because we never keep
: them for more than 24hours so I cannot answer that.
|
s******y
发帖数: 28562 | 5 two hours is nothing.
hours
【在 h***9 的大作中提到】
: thank you. they are tissue slides. I left them in 100% ethanol for two hours
: . they seemed to be fine.
:
: keep
|
D******9
发帖数: 2665 | |
