E****A 发帖数: 184 | 1 Ambion的strip-ez RNA kit不卖了,真郁闷,真不知道那么好用的kit为啥不卖了。给
ambion发信问原因,说一个工作日会给回信的,结果三四天了还不理我。
我是用dig-utp,如果用strip-ez kit的话,在68度incubate 10min就行,而且deprobe
效果非常非常好。现在这个kit不卖了,也没找到相关替代品。大家都是怎么deprobe呢?
80度煮两个小时?那膜能用几次啊? |
E****A 发帖数: 184 | 2 顶一个
deprobe
呢?
【在 E****A 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Ambion的strip-ez RNA kit不卖了,真郁闷,真不知道那么好用的kit为啥不卖了。给 : ambion发信问原因,说一个工作日会给回信的,结果三四天了还不理我。 : 我是用dig-utp,如果用strip-ez kit的话,在68度incubate 10min就行,而且deprobe : 效果非常非常好。现在这个kit不卖了,也没找到相关替代品。大家都是怎么deprobe呢? : 80度煮两个小时?那膜能用几次啊?
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w*****n 发帖数: 107 | 3 we usually strip the membrane by boiling in 0.1X SSC/0.1% SDS twice in a
microwave oven (each time 8 min). It works great. For the membranes that was
stored for 4 years, the signal was still very good. |
E****A 发帖数: 184 | 4 what kind of probe did you use? p32 or dig-utp? we use dig-utp
was
【在 w*****n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : we usually strip the membrane by boiling in 0.1X SSC/0.1% SDS twice in a : microwave oven (each time 8 min). It works great. For the membranes that was : stored for 4 years, the signal was still very good.
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