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发帖数: 906 | 1 Is this true?
In T7 RNA polymerase negative E. coli strain, T7 promoter-driven gene could
be expressed at a low level by other (prophage) RNA polymerases.
Anyone observed/heard the expression of T7 promoter-driven gene on high-copy
plasmid in T7 RNA polymerase negative E. coli strains?
Thanks. | a***e
发帖数: 1010 | 2 there is always T7 background expression. If that is a concern, try use
BL21(DE3)pLys |
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