g***e
发帖数: 4 | 1 最近出现怪现象,做了个siRNA,对tube formation促进,对transwell的migration却抑制
,那到底算促进angiogenesis还是抑制呀,一般不都两实验同样趋势么? | m*********7
发帖数: 5207 | 2 Have you tested the effect of siRNA on cell proliferation? How about an
alternative way to measure cell migration, such as scratch wound assay? | g***e
发帖数: 4 | 3 我也怀疑呢,没测proliferation,划痕就做了一次,似乎趋势和transwell一样.
我看有人说matrigel tube formation和细胞的侵袭,运动,还有什么什么有关,但我也在
很久以前貌似看到有篇很老的文献说迁移得弱的tube formation反而强,还说tube
formation不等同于在体的血管发育中的任何过程,只是一个间接反映细胞功能的指标.
但是这回出了这结果,再一查文献,都说迁移弱的tube formation也弱,简直是,难道当初
是做梦?
现在简直哪个结果都怀疑,恨不得都重新做一回. | g***e
发帖数: 4 | 4 我也怀疑呢,没测proliferation,划痕就做了一次,似乎趋势和transwell一样.
我看有人说matrigel tube formation和细胞的侵袭,运动,还有什么什么有关,但我也在
很久以前貌似看到有篇很老的文献说迁移得弱的tube formation反而强,还说tube
formation不等同于在体的血管发育中的任何过程,只是一个间接反映细胞功能的指标.
但是这回出了这结果,再一查文献,都说迁移弱的tube formation也弱,简直是,难道当初
是做梦?
现在简直哪个结果都怀疑,恨不得都重新做一回. | s******y
发帖数: 28562 | 5 In principle, matrigel tube formation has something to do with cell invasion
, including the secretion of MMP and the formation of invapodia.
However the transwell migration reflect the overall chemotaxix property that
has very little relevence to invapodia.
These two assays measure very different aspect of cell motility. Therefore,
tube formation has nothing to do with transwell asssay, in principle.
Thise people who claimed they have a relationship are simply saying whatever
that is convenient
【在 g***e 的大作中提到】
: 我也怀疑呢,没测proliferation,划痕就做了一次,似乎趋势和transwell一样.
: 我看有人说matrigel tube formation和细胞的侵袭,运动,还有什么什么有关,但我也在
: 很久以前貌似看到有篇很老的文献说迁移得弱的tube formation反而强,还说tube
: formation不等同于在体的血管发育中的任何过程,只是一个间接反映细胞功能的指标.
: 但是这回出了这结果,再一查文献,都说迁移弱的tube formation也弱,简直是,难道当初
: 是做梦?
: 现在简直哪个结果都怀疑,恨不得都重新做一回.
| g***e
发帖数: 4 | 6 谢谢回复,又看了看文献,我现在有些怀疑tube formation的增强是由于stress fiber被
抑制了(然而尚无证据,纯yy),但是文献净是说stress fiber促进tube formation(说来
说去就围绕vegf一个例子).我相信促进stress fiber的可能促进angiogenesis,然而仅
对于matrigel这个模型,我认为抑制stress fiber的促进tube formation,这是模型和实
际的差别(同样尚无证据,纯yy) | s******y
发帖数: 28562 | 7 Those so-called "stress fiber" concept does not have a good definition and
many
so-called stress fiber in many paper has no direct relevance to their study.
So don't take it too serious.
In my general understanding, NONE of those cellular structure (such as
lamelapodia or filopodia or stress fiber) can be clearly defined to have any
what-so-ever relationship with tube formation. Many of those papers just
related them for the convenience of their own theory which is not necessary
the truth
【在 g***e 的大作中提到】
: 谢谢回复,又看了看文献,我现在有些怀疑tube formation的增强是由于stress fiber被
: 抑制了(然而尚无证据,纯yy),但是文献净是说stress fiber促进tube formation(说来
: 说去就围绕vegf一个例子).我相信促进stress fiber的可能促进angiogenesis,然而仅
: 对于matrigel这个模型,我认为抑制stress fiber的促进tube formation,这是模型和实
: 际的差别(同样尚无证据,纯yy)
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