g***1 发帖数: 24 | 1 Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences! |
y******8 发帖数: 1764 | 2 4-5% resolving gel and transfer buffer without methanol. |
v***a 发帖数: 1242 | 3 without methanol是什么原理啊?
【在 y******8 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 4-5% resolving gel and transfer buffer without methanol.
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i********e 发帖数: 50 | 4 I use 6% separation gel and transfer without methanol for one hour at 100 V
. My protein is 550 KDa. |
s******r 发帖数: 2876 | 5 invirogen 有 Tis-acetate gel,据广告适合大蛋白。
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences!
【在 g***1 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Does anyone have experiences in western blot handling high molecular weight : (250~360 kDa) proteins? I met difficulties in transfer, thank in advance for : sharing your experiences!
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i*********t 发帖数: 78 | 6 add SDS in trnasfer buffer. |
p********t 发帖数: 411 | 7 Are you labeling Ryanoding Receptor?
V
【在 i********e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I use 6% separation gel and transfer without methanol for one hour at 100 V : . My protein is 550 KDa.
|