j********r
发帖数: 156 | 1 Is there any disadvantage of using siRNA transfection for some functional
assay, such as cell viability or cell death? Can anyone recommend a vendor
for
synthesize dsRNA (if the target sequence is already known for efficient
knockdown, based on the use of shRNA)? Many thanks,
| V*f
发帖数: 171 | 2 siRNA transfection only gives transient knockdown (3-7 days). If the
phenotype takes longer than 3-7 days to show, then siRNA hardly works.
Also some cell types are not easy to transfect.
Dharmacon and Ambion are good companies for siRNAs. | j********r
发帖数: 156 | 3 Thanks. So if I get very quick phenotypes in cultured cells using siRNA
transfection, will the data be convincing? Do I still need stable shRNA (
regular of conditionally if it is lethal) to confirm the results?
It seems there are also disadvantages of shRNA, such as in some cases
sequence-specific toxicity (probably due to overloading the RNAi machineary)
. But I am not sure about the down side of using siRNA. |
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