R****n 发帖数: 708 | 1 I'd like to analyze a DNA fragment from genomic DNA. No PCR, The length is
around 100-300bp. How can I selectively extract the DNA fragment from
genomic DNA.Thanks |
n***w 发帖数: 2405 | 2 sonication?
I don't know... |
R****n 发帖数: 708 | 3 I am thinking about randomly break the genomic DNA by multi DNase. Then use
a oligonucleotide probe to capture the target strand. But i am not sure if
there are other smarter way. |
s******s 发帖数: 13035 | 4 没更好的方法了。打碎了用biotin-RNA probe pull下来吧
use
【在 R****n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I am thinking about randomly break the genomic DNA by multi DNase. Then use : a oligonucleotide probe to capture the target strand. But i am not sure if : there are other smarter way.
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G***y 发帖数: 1082 | 5 2nd this.
【在 s******s 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 没更好的方法了。打碎了用biotin-RNA probe pull下来吧 : : use
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R****n 发帖数: 708 | 6 can you show me a protocol for this?
【在 s******s 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 没更好的方法了。打碎了用biotin-RNA probe pull下来吧 : : use
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