n***w
发帖数: 2405 | 1 Hi, all,
I have a simple question but I do not know the solution...
I am trying to figure out how to do multiple sequence alignment using the
online tool from EMBL.
I always get the warning from the 1st step that at least two sequences are
needed...
I do not know how to fix this since I already put two sequences...
Probably I used the wrong format for that.
Thank you all... |
A********0
发帖数: 3310 | 2 你有没有用对 file's format?
It works well to me.
【在 n***w 的大作中提到】
: Hi, all,
: I have a simple question but I do not know the solution...
: I am trying to figure out how to do multiple sequence alignment using the
: online tool from EMBL.
: I always get the warning from the 1st step that at least two sequences are
: needed...
: I do not know how to fix this since I already put two sequences...
: Probably I used the wrong format for that.
: Thank you all...
|
n***w
发帖数: 2405 | 3 That's probably the thing...
Would you mind sending me a sample file? Thank you...
【在 A********0 的大作中提到】
: 你有没有用对 file's format?
: It works well to me.
|
n***w
发帖数: 2405 | 4 That's probably the thing...
Would you mind sending me a sample file? Thank you...
【在 A********0 的大作中提到】
: 你有没有用对 file's format?
: It works well to me.
|
l*****a
发帖数: 1431 | 5 did you put > at the beginning of each sequencing? It should be like this
>name1
sequence1
>name2
sequence2
.... |
A********0
发帖数: 3310 | 6 correct. or try this.
>A.DE.x.IFA86_Z30637
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCTAAGA
AAGCTAAGGGTTGGTTTTATAGACATCACTTTGAATGTAGGCATCCAAAA
GCAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCACTTGGGTCAGG
GGGTCTCCATAGAATGGAGGCAGAAAAGATATAGCACACAAGTAGATCCT
GACCTAGCAGACCAACTGATTCACCTGCATTATTTTGACTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAGGAGAAGTAGTTAGACCTAGGTGTG
AATATCAAGCAGGACATAACAAGGTAGGATCACTGCAATATTTGGCACTG
AAAGCATTAGTAGCACCAAAAAAGGCAAAGCCACCTTTGCCTAGTCTTAA
GAAGTTAACAGAAGATAGATGGAACGAGCCCCAGAAGACCAGGGGCCACA
GAGGGAACCGTTCAATGAATGGACACTAG
>A.DE.x.K15DE_AF200476
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCTAAGA
AAGCTAAGGGTTGGTTTTATAGACATCACTTTGAATGTAGGCATCCAAAA
GCAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCACTTGGGTCAGG
GGGTCTCCATAGAATGGAGGCAGAAAAGATATAGCACACAAGTAGATCCT
GACCTAGCAGACCAACTGATTCACCTGTATTATTTTGATTGTTTTTCAGA
CTCTGCCATAAGAAAAGCCATATTAGGAGAAATAGTTAGACCTAGGTGTG
AATATCAAGCAGGACATAATAAGGTAGGATCGCTACAATATTTGGCACTG
AAAGCATTAGTAGCACCAAAAAAGACAAAGCCACCTTTGCCTAGTCTTAA
GAAGTTAGCAGAAGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCACA
GAGGGAACCGTTCAATGAATGGACACTAG
>A.DE.x.K19DE_AF200477
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATATCTCAAAGA
AAGCAAAAAATTGGTTTTATAGACATCACTTTGAAAGCAGTCATCCAAGA
GTAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAAAGATTGGCACTTGGGTAATG
GGGTCTCCATAGAATGGAGACTAAGAAGATATAGCACACAAATAGATCCT
GACCTGGCAGACCAACTAATTCATCTGCATTATTTTAATTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAGGACAAGTAGTTAGACCTAGGTGTG
ACTATCAAGCAGGACATAACAAGGTAGGATCTCTACAATATTTGGCACTG
AAAGCATTAGTAACACCAGTAAGGACAAGGCCACCTTTGCCTAGTGTTAG
GAAATTAGCAGAGGACAGATGGAACAAGCCCCAGAAGACCAGGGGCCCCA
GAGGGAGCCATACAATGAATGGACATTAG
>A.GE.99.99GEMZ011_DQ207944
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCAAAGA
AAGCTAGAGAGTGGTTTTATAGACATCACTATGAAAGCAGGCATCCAAAA
ATAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGGCTAGTAGTAAA
AACATATTGGGGTCTGCATGCAGGAGAAAAAGACTGGCACTTGGGTCATG
GGGTCTCCATAGAATGGAGACAGAAAAGGTATAGCACACAAATAGACCCT
GACCTGGCGGACCAACTAATTCATCTGCATTATTTTGACTGTTTTTCAGA
ATCTGCCATAAGGAAAGCCATAGTAGGACACATAGTTAGCCCTAAGTGTA
ACTATCCACCAGGACACAGCAAGGTAGGATCTCTACAATACTTGGCACTG
AAAGCATTAGTAACACCAACAAGAGAAAGGCCACCTTTGCCTAGTGTTAG
AAAGCTAACAGAGGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCGCA
GAGGGAACCACACAATGAATGGATGTTAG
>A.IN.01.1579A_DQ083238
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGCACAGTTTAGTAAAACATCATATGTATGTCTCAAAGA
AAGCAAAAAATTGGGTTTATAAACATCACTTTGAAAGCAGGCATCCAAGA
GTAAGTTCAGAAGTACACATCCCACTAGGAGAAGCTAGATTAGTGGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAAAGACTGGCACTTGGGTCATG
GGGTCTCCATAGAATGGAAGCTAAGAGGATATAACACACAAGTAGATCCT
GACCAGGCAGACCAACTAATTCATCTGCATCATTTTGACTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAAAACAAGTAGTTAGCCCTAAGTGTG
AATATCAAACAGGACATAACAAGGTAGGATCTCTACAATATTTAGCACTA
AAAGCATTAGTAACACCAACAAAGGCAAAGCCACCTTTGCCTAGTGTTAG
GATATTAGCAGAGGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCCCA
GAGAGAGCCATACAATGAATGGATGTTAG
【在 l*****a 的大作中提到】
: did you put > at the beginning of each sequencing? It should be like this
: >name1
: sequence1
: >name2
: sequence2
: ....
|
n***w
发帖数: 2405 | 7 Thank you all!!!
It worked!!! |
a*****y
发帖数: 277 | |
T**********t
发帖数: 1604 | |
