m*p 发帖数: 226 | 1 想把基因表达在核里。谁有Nulcear Localization Signaling Sequence?, please
post it here. 谢过了! |
m******5 发帖数: 1383 | 2 google
Large T antigen NLS
one of the most commonly used NLS sequence |
m*p 发帖数: 226 | 3 Thanks!
Anybody knows a tag that can drive the protein to the nucleus?
Has anybody use H2b(his-58) ?
先谢了! |
F*****e 发帖数: 182 | 4 我们用H2B-GFP做的knock-in老鼠,在核内表达的挺好 |
m*p 发帖数: 226 | 5 那是当然。 Histone 2B has to be expressed in nucleus and binds to DNA.
Thanks though!
【在 F*****e 的大作中提到】 : 我们用H2B-GFP做的knock-in老鼠,在核内表达的挺好
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