c***c
发帖数: 113 | 1 Hello all.
I will start to do DNA methylation assay, I am totally new to this technique
. Could anyone please tell me which method (or kit) is the most reliable and
currently used one?
Thanks. |
d****t
发帖数: 139 | 2 3H filter paper binding assay |
c*********t
发帖数: 340 | 3 sample number? do you want to just look a few loci or have a genomic-scale
view? MethyLight works especially well for me for the former purpose, and
Illumina Infinium chip for the latter (not so satisfactorily, but ok)
FYI:
http://www.nature.com/nrg/journal/v11/n3/abs/nrg2732.html?lang= |
b****r
发帖数: 17995 | 4 这确实要看你到底想分析什么有何目的，否则很难给出有意义的答案 |
c***c
发帖数: 113 | 5 Thank you all very much.
Our purpose want to the methylation status at the promoter region of
interest gene, we may evaluate several CpG islands at different time points.
I have checked the onestep qMethy kit from Zymoresearch, it looks good. Did
anyone here use this kit? |
b****r
发帖数: 17995 | 6 如果你们lab以前没发过什么methylation方面的文章，还是别轻易尝试什么新方法吧
为啥不用经典的pyro sequencing
points.
Did
【在 c***c 的大作中提到】
: Thank you all very much.
: Our purpose want to the methylation status at the promoter region of
: interest gene, we may evaluate several CpG islands at different time points.
: I have checked the onestep qMethy kit from Zymoresearch, it looks good. Did
: anyone here use this kit?
|
c***c
发帖数: 113 | 7 Thanks.
I read something about Pyro sequencing online, it seems to do pyro
sequencing, special instrument and reagents need to be bought. Could you
please tell me what do you have for pyro sequencing assay and how did you
design the primers? Currently we just want to check one gene of interest, we
will not do DNA methylation assay routinely.
【在 b****r 的大作中提到】
: 如果你们lab以前没发过什么methylation方面的文章，还是别轻易尝试什么新方法吧
: 为啥不用经典的pyro sequencing
:
: points.
: Did
|
