S*****s
发帖数: 287 | 1 I need to dissect out microstructures of mouse brain, such as cerebellum,
hippocampus, thalamus, etc. We have never done this in our lab before, so
our tools suck. But I do not want to waste money to order a random selection
on Fisher or VWR catalog. There are so many neuroscientists on this board.
Could you please let me know what are your favorite microdissection tools? I
need to order all the surgical tools to remove the head, take out brain,
and do actual dissection to get the substructures. Your input is greatly
appreciated. Thanks! |
w*******2
发帖数: 435 | 2 http://www.finescience.com
91460-11 x1
11150-10 x2
11255-20 x2
91500-09 x1
That all you need. |
S*****s
发帖数: 287 | 3 Thanks for your suggestion! This list is of great help. What scissors do you
use to cut the head off? And, do you use the thin wax board for each brain?
If so, do you happen to know the ordering information? Thanks a lot!
【在 w*******2 的大作中提到】
: http://www.finescience.com
: 91460-11 x1
: 11150-10 x2
: 11255-20 x2
: 91500-09 x1
: That all you need.
|
w*******2
发帖数: 435 | 4 You don't need to cut the head off.\
you can use 91460-11 x1 to cut open skull from the top carefully without
damage the brain.Then take the whole brain off.
I have no idea why you need thin wax board.
We put 2% agarose in a small tissue culture dish.You can put the brain with
PBS in the dish for farther micro-dissection with tool 11255-20 x2
91500-09 x1.
good luck. |
S*****s
发帖数: 287 | 5 What a good idea! 2% agarose in a petridish should be much cheaper than
those wax boards. I guess I just need to order some cheap agarose powder.
Thanks a lot for your help. I really appreciate!
with
【在 w*******2 的大作中提到】
: You don't need to cut the head off.\
: you can use 91460-11 x1 to cut open skull from the top carefully without
: damage the brain.Then take the whole brain off.
: I have no idea why you need thin wax board.
: We put 2% agarose in a small tissue culture dish.You can put the brain with
: PBS in the dish for farther micro-dissection with tool 11255-20 x2
: 91500-09 x1.
: good luck.
|
r*****m
发帖数: 231 | 6 Do you have someone to teach you how to do this? If you don't even know
which tools to use, I don't see the actual dissection as a trivial task for
you... |
S*****s
发帖数: 287 | 7 Thanks for the concern. My friend showed me how to do it, and I actually
practiced several animals. The only problem was that our tools suck. We did
not maintain our tools OK so they are rusted or bended. I would like to
replace them all. I am glad that wangchao2 gave me so detailed information.
That was very helpful. Thanks guys!
for
【在 r*****m 的大作中提到】
: Do you have someone to teach you how to do this? If you don't even know
: which tools to use, I don't see the actual dissection as a trivial task for
: you...
|
D*a
发帖数: 6830 | 8 解刨过一些brain，这个wax boards是干嘛用的？我都是直接放在culture dish上的，
有什么区别么？thanks
with
【在 w*******2 的大作中提到】
: You don't need to cut the head off.\
: you can use 91460-11 x1 to cut open skull from the top carefully without
: damage the brain.Then take the whole brain off.
: I have no idea why you need thin wax board.
: We put 2% agarose in a small tissue culture dish.You can put the brain with
: PBS in the dish for farther micro-dissection with tool 11255-20 x2
: 91500-09 x1.
: good luck.
|
y**u
发帖数: 7459 | 9 我倒是用这个，粉红色的，叫dental wax， 有硬的和软的。主要是要用scalpel切什么
的时候垫在底下。也不是必须。
【在 D*a 的大作中提到】
: 解刨过一些brain，这个wax boards是干嘛用的？我都是直接放在culture dish上的，
: 有什么区别么？thanks
:
: with
|
z****u
发帖数: 1007 | 10 microdissection是我们的daily work. 在dissection microsope下面解剖E10.5 mouse
embryo的first branchial arch..整个长度不超过2mm吧。然后把distal part ,
proximal part 分开，体外培养。
楼主需要的agar dish.2% agar加入碳粉让它变黑，然后autoclave之后铺petri dish.
black agar dish 背景解剖容易得多。
finescience的那些工具不错，我们一直都用它。
一定需要人带着做一遍。 |
D*a
发帖数: 6830 | 11 主要是想知道是干嘛用的？对解刨有什么作用呢？
要是有用的话，我就去弄点agarose，也不麻烦。
【在 y**u 的大作中提到】
: 我倒是用这个，粉红色的，叫dental wax， 有硬的和软的。主要是要用scalpel切什么
: 的时候垫在底下。也不是必须。
|
S*****s
发帖数: 287 | 12 谢谢大家了。我一个朋友是这方面的专家，她已经带着我做过一次 Dissection，很让
我开眼界。看完了我自己试了几只老鼠，和牛人学过感觉就是不一样。现我们实验室的
解剖工具已经被折磨的不像样子了，尖头的镊子剪刀头都断了，剪刀也都是锈迹斑斑，
所以想订一些新的更换一下。
昨天我已经感叹过王朝兄的 2% agarose 了。我以前看朋友做这个实验都是垫粉红色的
蜡板，我觉得好处就是不粘组织，而且下刀的时候组织下面垫一个软的东西比较好切一
点。这东西也不是必须，不过用 2% agarose 的话比蜡板肯定便宜很多。今天看到
zhaohu 朋友的建议又让我拍了下桌子。就说版上牛人多！这些经验不具体做这个实验
肯定不知道。非常感谢！
朋友建议我订一个 dissection microscope，英文叫 stereomicroscope。我看了一下
Fisher 的网站，一个 Leica 的 S4 两千不到，S8 四千不到。不知道这个是不是必须
要订的？上周的解剖我用肉眼做的，看起来的确有点吃力，不过也不是完全搞不定。请
问一下各位朋友你们是直接用肉眼还是用哪种解剖显微镜？谢谢。 |
m*********n
发帖数: 215 | 13 stereomicroscope，那个有自带的黑色背景台。#10 surgical blade和＃5 镊子足够了。E12.5到p14都做过，在dish中加1Xpbs dissect。cerebellum, thalamus还有hippocampis都是这么明显的结构。。。 |
